Agbl3 (NM_178630) Mouse Tagged ORF Clone

CAT#: MR218893

Agbl3 (Myc-DDK-tagged) - Mouse ATP/GTP binding protein-like 3 (Agbl3)

Product Images

Circular map for MR218893

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Agbl3
• Synonyms: 2900053G10Rik; 4930431N21Rik; 6530406M24Rik; CCP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218893 representing NM_178630
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGAAGATTCAGAGGAGGAAGACTACTCAGATAGAAGCATCAGTGATGACGATGACTTGGATGAGG
  ATTCCTTCATGAAATTTGTAAGTGATGATATTCATCCGTGCACACTACTGGCAGCTGATTCTATTGGTGA
  CCCATTCTTCCCTCGGACTACACAAATTTTATTGGAGTATCAGCTAGGAAGATGGGTACCACGCCTTCGC
  GGACCACGAGATTTATATGGTGTCTCTTCTTCTGGTCCACTGAGCCCAACACGGTGGCCATACCACTGTG
  AGGTCATTGATGAAAAAGTCCAGCATATTGAATGGACTCCCTTTGTCCCTGAGCCAGTGTATGTCCCGAC
  AGGCCTGGAAATAGAGCCTGTTTACCCAAACTCCAAGGAAGATACTGTGGTTTATTTAGCTGAAGATGCT
  TACAAAGAGCCCTGTTTTGTGTATTCCCGAGTTGGGGGTAACCGAACATCCTTGAAACAACCTGTGGATA
  ACTGTGACAACACGCTGGTGTTTGAAGCAAGGTTTGAGAGTGGTAATCTACAGAAGGTAGTCAAAGTTGC
  AGATCATGAATACGAGTTGACTGTGCGCCCAGACCTCTTCACAAATAAGCACACTCAGTGGTACTATTTC
  CAGGTCACTAATACCCAAGCAGAAATAGTCTACAGATTCACTATTGTCAACTTTACCAAGCCTGCTAGTC
  TTTACAATCGGGGTATGAAGCCATTGTTCTATTCTGAAAAAGAGGCCAAAACCCATAACATTGGCTGGCA
  GAGAATAGGAGATCAAATCAAGTATTATAAAAATAACCTTGGGCAAGATGGGCGCCATTTTTTTTCTCTT
  ACGTGGACATTTCAGTTTCCACACAGCCAGGATACGTGCTACTTTGCTCACTGCTATCCATACACTTACT
  CCAACCTTCAAGAATACCTGTCTGGCATCAACAGTGACCCAGTAAGATCAAAGTTTTGTAAAATACGTGT
  CTTGTGTCACACACTTGCCAGGAATATGGTGTATGTCTTGACAATAACTACTCCCTTGAAGACTAGTGAC
  TCAAAGCGCAAGGCTGTGATTTTGACTGCAAGGGTCCATCCAGGAGAGACCAACAGCTCTTGGATCATGA
  AAGGCTTCCTGGATTATATTTTAGGAGACTCAAGTGATGCACGCTTGCTTCGGGACACTTTCATCTTCAA
  GGTGGTACCCATGCTGAATCCAGATGGCGTGATTGTGGGAAATTATCGATGTTCCTTAGCTGGACGGGAT
  TTAAACCGTAATTACACATCGCTCCTGAAGGAGTCTTTCCCTTCTGTTTGGTATACAAGGAACATGATCA
  ATAGGTTAATGGAGAAACGAGAGGTTATTTTATATTGTGATCTTCATGGCCATAGTAGGAAACAGAATAT
  TTTCATGTATGGTTGTGATGGTAGCAGCAGATCTAAAACAAAAGGATTATACTTACAGCAACGGATCTTT
  CCACTCATGCTGAGCAAAAATTGCCCAAATATATTTTCCTTCTCAGCTTGCAAGTTTAACGTTCAGAAAA
  GCAAAGAGGGGACAGGAAGGGTTGTAATGTGGAAGATGGGCATCAGGAACAGCTTCACGCTGGAGGCCAC
  CTTCTGCGGATCTACTCTGGGCAATAAACGAGGCACTCATTTCGGCACAAAAGATTTAGAGTCGATGGGA
  TATCATTTTTGTGATTCTCTGTTGGACTACTGTGATCCTGACAGGAGCAAATATTATCAGTGCTTGAAAG
  AATTAGAAGAAATGGAGAAACATTTGAGCTCCGAAAGAGTCTCTGATAACACAGATACTTCTCTAGTAGA
  AATTTCTTTGGATGTAGAGTCTAGTAGTCGAGGCTCTGACAGCTCAGAGTCCAATGACACTCAGACTTAC
  CTTCTCAAGGTGACCTCTCAGGCAAGAAACAAGAAAAAGTATCTGAAAACAAAGAGGGAAAGAAATGCCA
  TCCTAGCAAACTGCCAGAATAACATGCAAGAGGTTTATGGCAAAGAACATTTACTCCAAAGACACGATGA
  ATCAAATTCTGATGGGAATGATCCAAGGATAGATGCACCAGATGTTTATGTGGCTCACTGTTTCAGAAGA
  CCGTTGCCTAACCAAGGCGTGGTGAAAATTCCTGGACAAAGGTTTTATCCCGGAAAAACATGGTCCAGCT
  CCCAGCGTATGATTAAGAGTCTTAACAAAGACCATAGGACATGTATTTTAGAAACTTGTAAGAATCCAAT
  CCAGGAAGTTCAGTCCAGAGGAATCAATATACATGAAAGTTGTTTCAAAATGGCGAAATGTCCTATGAAC
  AAAAGACCTTCACATTGGATTGAGAAGACAAGAATACCAACAGAGTCACATCACCAGTTGAAAAGCAAAG
  CAAAAAGATGCTCGTCTTTCCAAAGCAAGAGGACTGGGACGAATTGGACAGATGATGAGAAACGAATTTA
  CAGGGATAAAAGAATAGCTCAAACTCAGGAAATATTAAAGTATTTACTCCCTATCGTGGAGTCCTCTCAA
  AACAGGAAATCTACTCAAATGAACAATCTAATCAACCCGATAGCCAACTTGCAACAGCATCAACTTATTC
  CAACTGCTTGCATAAATAGAAGAAGATACAGCATACCTTGGACACCAACCAGAAATCTCCCTTTTAAAGC
  CCAAAGGAATCTTATGACAGACACCTCCGAATGGCTCCAGTCTGTGCCCCTTGGGTCATTTGAAAGTTTG
  TTACCTCTCTGCAATCTTCAGAAGAAGACAAAACATTTTGAACTCTGGGGCAAAAAGGCTAAGGATGTGC
  AGCTTGCCACCAGCCAATGGGAGGCTGTGCCCTTGAGTTCTAACATGGATGCAAGTATCATCAGGGGTAA
  CAGTGTTCTGCAGCCTAAAGAATTCACCATGAGGAGCTCTAAGCAGAGAATTCCTTACCTAACCAAAACC
  AGCAAGAAACCCAGTGAGAGTGATGGCCTGCTCACCTTCCAGCTGAAGATTCACAGGAACAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218893 representing NM_178630
  Red=Cloning site Green=Tags(s)
  
  MSEDSEEEDYSDRSISDDDDLDEDSFMKFVSDDIHPCTLLAADSIGDPFFPRTTQILLEYQLGRWVPRLR
  GPRDLYGVSSSGPLSPTRWPYHCEVIDEKVQHIEWTPFVPEPVYVPTGLEIEPVYPNSKEDTVVYLAEDA
  YKEPCFVYSRVGGNRTSLKQPVDNCDNTLVFEARFESGNLQKVVKVADHEYELTVRPDLFTNKHTQWYYF
  QVTNTQAEIVYRFTIVNFTKPASLYNRGMKPLFYSEKEAKTHNIGWQRIGDQIKYYKNNLGQDGRHFFSL
  TWTFQFPHSQDTCYFAHCYPYTYSNLQEYLSGINSDPVRSKFCKIRVLCHTLARNMVYVLTITTPLKTSD
  SKRKAVILTARVHPGETNSSWIMKGFLDYILGDSSDARLLRDTFIFKVVPMLNPDGVIVGNYRCSLAGRD
  LNRNYTSLLKESFPSVWYTRNMINRLMEKREVILYCDLHGHSRKQNIFMYGCDGSSRSKTKGLYLQQRIF
  PLMLSKNCPNIFSFSACKFNVQKSKEGTGRVVMWKMGIRNSFTLEATFCGSTLGNKRGTHFGTKDLESMG
  YHFCDSLLDYCDPDRSKYYQCLKELEEMEKHLSSERVSDNTDTSLVEISLDVESSSRGSDSSESNDTQTY
  LLKVTSQARNKKKYLKTKRERNAILANCQNNMQEVYGKEHLLQRHDESNSDGNDPRIDAPDVYVAHCFRR
  PLPNQGVVKIPGQRFYPGKTWSSSQRMIKSLNKDHRTCILETCKNPIQEVQSRGINIHESCFKMAKCPMN
  KRPSHWIEKTRIPTESHHQLKSKAKRCSSFQSKRTGTNWTDDEKRIYRDKRIAQTQEILKYLLPIVESSQ
  NRKSTQMNNLINPIANLQQHQLIPTACINRRRYSIPWTPTRNLPFKAQRNLMTDTSEWLQSVPLGSFESL
  LPLCNLQKKTKHFELWGKKAKDVQLATSQWEAVPLSSNMDASIIRGNSVLQPKEFTMRSSKQRIPYLTKT
  SKKPSESDGLLTFQLKIHRNS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178630
• ORF Size: 3003 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178630.4, NP_848745.2
• RefSeq Size: 5246 bp
• RefSeq ORF: 3006 bp
• Locus ID: 76223
• UniProt ID: Q8CDP0
• Cytogenetics: 6 B1
• MW: 115.8 kDa
• Gene Summary: Metallocarboxypeptidase that mediates both deglutamylation and deaspartylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates or polyaspartates from the carboxy-terminus of target proteins such as MYLK. Does not show detyrosinase or deglycylase activities from the carboxy-terminus of tubulin.[UniProtKB/Swiss-Prot Function]