Nemf (NM_025441) Mouse Tagged ORF Clone

CAT#: MR218737

Nemf (Myc-DDK-tagged) - Mouse nuclear export mediator factor (Nemf)

Product Images

Circular map for MR218737

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: Nemf
• Synonyms: 1500011I12Rik; 4933405E14Rik; Sdccag1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218737 representing NM_025441
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAGCCGGTTCAGCACGGTTGACCTCCGCGCAGTGCTCGCGGAACTGAATGCGAGCTTGCTAGGAA
  TGAGAGTAAACAACGTTTATGATGTGGATAACAAGACATATCTTATTCGGCTTCAAAAACCTGATTTTAA
  AGCTACACTGTTACTTGAGTCTGGTATACGTATTCATACAACAGAATTTGAGTGGCCTAAGAACATGATG
  CCGTCGAGTTTTGCCATGAAGTGTCGAAAACATTTGAAGAGTCGGAGATTAGTCAGTGCAAAACAGCTTG
  GTGTGGACAGAATTGTGGATTTCCAGTTTGGAAGTGACGAAGCTGCTTATCACTTAATCATTGAGCTCTA
  TGACAGGGGGAACATTGTTCTTACAGATTATGAGTACCTAATTTTAAATATCCTAAGGTTTCGAACTGAT
  GAAGCAGATGATGTTAAGTTTGCTGTTCGTGAACGCTATCCCATAGATCATGCCAGAGCTGCTGAACCTT
  TGCTGACTTTGGAAAGGTTGACTGAAGTAATAGCTGCTGCTCCTAAAGGTGAAGTGCTGAAGAGAGTTCT
  TAACCCATTACTTCCCTATGGACCAGCTCTTATTGAGCACTGTCTTATAGAAAGTGGATTCTCTGGCAAT
  GCTAAAGTGGATGAAAAACTTGAGAGCAAAGATATTGAAAAGATACTGGTTTGTGTACAGAGGGCAGAAG
  ACTATTTGAGGAAAACATCCAACTTCAATGGAAAGGGCTATATAATTCAGAAAAGAGAGGCAAAACCAAG
  CCTGGATGCAGATAAACCAGCTGAAGACATACTCACGTATGAGGAGTTTCATCCTTTCTTGTTTTCTCAG
  CATTTACAATGTCCATATATAGAATTTGAATCATTTGACAAGGCCGTGGATGAATTTTATTCCAAGATAG
  AAGGGCAGAAAATTGACTTAAAAGCTTTACAACAGGAAAAACAAGCACTGAAGAAATTAGATAATGTCCG
  AAAGGATCATGAAAACAGATTAGAAGCTCTTCAGCAGGCTCAGGAAATTGATAAACTGAAAGGGGAGCTC
  ATAGAGATGAACCTGCAGATAGTGGACAGAGCCATTCAGGTAGTCCGAAGTGCGTTGGCCAACCAGATAG
  ACTGGACAGAAATCGGAGTGATTGTGAAAGAAGCTCAAGCTCAAGGAGACCCGGTTGCATGTGCAATCAA
  AGAGCTAAAACTGCAAACCAACCACGTTACGATGCTGCTACGAAATCCATACTTGTTATCAGAGGAGGAA
  GATGGTGATGGTGATGCCAGCATTGAGAACAGTGATGCTGAAGCACCAAAAGGGAAAAAAAAAAAGCAAA
  AGAACAAGCAGCTGCAGAAGCCGCAGAAGAACAAGCCGCTGCTTGTAGATGTGGATCTCAGCCTGTCAGC
  CTATGCCAATGCCAAAAAATATTATGATCATAAGAGGTATGCTGCTAAAAAAACCCAGAGGACTGTTGAA
  GCTGCTGAGAAGGCATTCAAATCAGCAGAAAAGAAAACAAAGCAAACCTTAAAAGAAGTACAAACAGTTA
  CTTCTATCCAAAAAGCAAGAAAAGTGTATTGGTTTGAGAAATTTCTGTGGTTCATTAGTTCAGAGAACTA
  TTTAATCATAGGTGGTCGGGATCAGCAGCAGAATGAGATTATTGTGAAAAGATACTTGACACCAGGAGAC
  ATTTATGTGCATGCTGATCTTCATGGAGCTACCAGCTGTGTAATTAAGAATCCGACAGGCGAACCCATCC
  CTCCACGGACTTTGACTGAAGCAGGCACAATGGCACTTTGCTACAGTGCTGCTTGGGATGCACGTGTTAT
  CACTAGTGCTTGGTGGGTGTACCATCATCAGGTGTCTAAAACAGCACCAACAGGAGAATATTTAACAACA
  GGAAGCTTCATGATAAGAGGAAAAAAGAATTTCCTTCCTCCTTCATACCTAATGATGGGGTTTAGCTTCC
  TTTTTAAGGTAGATGAGTCTTGTGTCTGGAGACATCGAGGTGAACGAAAAGTCAGAGTGCAGGATGAAGA
  CATGGAAACACTGACAAGCTGCACAAGTGAACTCATGGCAGAAGAAATGGAACAACTAGAAGGGGGTGAT
  AGCAGTGAAGAAGAGACTGAGGAATTACATGGAATGCCTGGAGACGTAGAACTCATGACTCAGGTTGATC
  AAGAGGACATTGCTGTGCACAGTGGGAGAGATGAACTAAGCTCTGAAGATGGAGAAGCCAAAGCAGTTAC
  AAAAGATCAGGAGCCCATTGGTGAAATGAAGGAAGAAGAGGAAGACACCTTTGAATATCCTGATACTACC
  ATTGACTTGTCCCATCTTCAGTCCCAACGGCCTCTACAGAAACTGGCTCCAAGAGAAGAGTCTTTGAACT
  CAAATGACAGTAAATCACAGGGCCGAAGACATTTGTCAGCCAAGGAGAGAAGAGAAATGAAAAAGAAAAA
  GCTCCCATGTGAGTCAGGGGATTTAGAAGTGATAGAAGAAAAGGACAAAGAGAGAGAAAGTGCTGTGCAC
  ACTGAAGCTTACCAAAACACAAGCAAAAATGTGGCAGCTGGACAGCCAATGAAAAGAGGACAGAAGAGTA
  AAATGAAAAAAATGAAGGAAAAATACAAAGACCAGGATGATGAAGATCGTGAACTTATTATGAAGTTGCT
  GGCATCTGCAGGTTCGAACAAAGAAGAAAAGGGGAAGAAAGGAAAGAAAGGAAAACCAAAAGATGAACCT
  GTGAAAAAACCCCCACAGAAACCAAGAGGTGGACAGCGGGTTTTAGATGTTGTTAAAGAACCTCCGTCCC
  TGCAGGTTTTAGCTCATGACTTACAAGACTTAGCTGTCGATGATCCACATGATGACAAGGAGGAACACGA
  TCTGGATCAGCAGGGGAATGAGGAAAATCTATTTGACTCTTTGACAGGGCAGCCACATCCTGAGGATGTA
  CTGATGTTTGCTATTCCAATATGTGCCCCTTACACCATCATGACAAACTATAAATACAAAGTGAAACTTA
  CACCTGGAGTTCAGAAAAAGGGGAAAGCTGCGAAGACAGCCTTGAACAGTTTCATGCACTCCAAAGAAGC
  CACAGCAAGAGAAAAAGACTTATTCCGAAGTGTGAAGGACACAGATTTATCAAGAAACATTCCTGGAAAA
  GTAAAAGTGTCCGCACCTAATCTTCTGCATGTGAAGAGGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218737 representing NM_025441
  Red=Cloning site Green=Tags(s)
  
  MKSRFSTVDLRAVLAELNASLLGMRVNNVYDVDNKTYLIRLQKPDFKATLLLESGIRIHTTEFEWPKNMM
  PSSFAMKCRKHLKSRRLVSAKQLGVDRIVDFQFGSDEAAYHLIIELYDRGNIVLTDYEYLILNILRFRTD
  EADDVKFAVRERYPIDHARAAEPLLTLERLTEVIAAAPKGEVLKRVLNPLLPYGPALIEHCLIESGFSGN
  AKVDEKLESKDIEKILVCVQRAEDYLRKTSNFNGKGYIIQKREAKPSLDADKPAEDILTYEEFHPFLFSQ
  HLQCPYIEFESFDKAVDEFYSKIEGQKIDLKALQQEKQALKKLDNVRKDHENRLEALQQAQEIDKLKGEL
  IEMNLQIVDRAIQVVRSALANQIDWTEIGVIVKEAQAQGDPVACAIKELKLQTNHVTMLLRNPYLLSEEE
  DGDGDASIENSDAEAPKGKKKKQKNKQLQKPQKNKPLLVDVDLSLSAYANAKKYYDHKRYAAKKTQRTVE
  AAEKAFKSAEKKTKQTLKEVQTVTSIQKARKVYWFEKFLWFISSENYLIIGGRDQQQNEIIVKRYLTPGD
  IYVHADLHGATSCVIKNPTGEPIPPRTLTEAGTMALCYSAAWDARVITSAWWVYHHQVSKTAPTGEYLTT
  GSFMIRGKKNFLPPSYLMMGFSFLFKVDESCVWRHRGERKVRVQDEDMETLTSCTSELMAEEMEQLEGGD
  SSEEETEELHGMPGDVELMTQVDQEDIAVHSGRDELSSEDGEAKAVTKDQEPIGEMKEEEEDTFEYPDTT
  IDLSHLQSQRPLQKLAPREESLNSNDSKSQGRRHLSAKERREMKKKKLPCESGDLEVIEEKDKERESAVH
  TEAYQNTSKNVAAGQPMKRGQKSKMKKMKEKYKDQDDEDRELIMKLLASAGSNKEEKGKKGKKGKPKDEP
  VKKPPQKPRGGQRVLDVVKEPPSLQVLAHDLQDLAVDDPHDDKEEHDLDQQGNEENLFDSLTGQPHPEDV
  LMFAIPICAPYTIMTNYKYKVKLTPGVQKKGKAAKTALNSFMHSKEATAREKDLFRSVKDTDLSRNIPGK
  VKVSAPNLLHVKRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025441
• ORF Size: 3192 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025441.3, NP_079717.2
• RefSeq Size: 3737 bp
• RefSeq ORF: 3195 bp
• Locus ID: 66244
• UniProt ID: Q8CCP0
• Cytogenetics: 12 C2
• MW: 121.2 kDa
• Gene Summary: Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. NEMF is responsible for selective recognition of stalled 60S subunits by recognizing an exposed, nascent chain-conjugated tRNA moiety. Nemf is important for the stable association of Ltn1 to the complex. May indirectly play a role in nuclear export.[UniProtKB/Swiss-Prot Function]