Arhgap45 (NM_001142701) Mouse Tagged ORF Clone

CAT#: MR218590

Hmha1 (Myc-DDK-tagged) - Mouse histocompatibility (minor) HA-1 (Hmha1), transcript variant 1

Product Images

Circular map for MR218590

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arhgap45
• Synonyms: 6330406L22Rik; AW539505; Ha-1; Hmha1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218590 representing NM_001142701
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTCTCGGAAGAAGCGAGAACTTATGAAAACTCCCTCTATTTCCAAAAAGAACCGCGCGGGGAGCC
  CCAACCCGCAGTCCTCCTCAGGGGAGCTGCCCAGGAAGGATTGGACAGAAGCTCCCGGCCTGGAGCCACC
  AGCCACATCCCTGTCCACGGTAGCCAAGGGCACAGGGACACTCAAAAGACCCACCAGCCTGAGCCGCCAT
  GCCAGTGCAGCAGGCTTCCCGCTCTCGGGCACTGCCACCTGGACCCTGGGCCGTGGGTACCGAAGCCCCC
  TTTCTGCGGCCAGTCCGGCAGAGTTACCCACTGAGGGAGCTTTCCCTGATGGCGTAGAAGACATCTCAAC
  CCTGCTGGCGGATGTGGCCCGCTTTGCTGAGGGCTTGGAGAAGCTCAAGGAGTGTGTGCTGCAGGACGAC
  CTTCTCGAGGCCCGCCGGCCACTGGCCCATGAGTGCCTGGGTGAAGCTCTGCGAGTCATGCGCCAGGTCA
  TCTCCAGATACCCACTCCTCAACACCGTGGAGACACTCACAGCCGCTGGTACACTCATTGCCAAGGTCAA
  AGCCTTTCATTATGAATGCAACAATGAATCAGATAAGAGGGAATTTGAGAAGGCTCTGGAAACCATTGCG
  GTGTCCTTCAGCTGCACTGTGTCCGAGTTCCTTTTGGGTGAAGTGGACAGCAGTACTCTCCTGGCGGTGC
  CTCCGGGGGACCCCAGCCAGTCCATGGAGAACCTTTATGGGGCTGGCACGGAGGGGCCCCCACATAACGT
  GGAGGAATGTGAAGAAGGTTGCCTGCCCCCCGAAGAGGTGGACATGCTTCTGCAGCGATGCGAAGGGGGT
  GTGGACGCTGCACTGCAGTATGCAAAAGACATGGCCAGGTACATGAAGGACCTCATCAGCTACCTGGAGA
  AGAGGACCACCCTGGAGATGGAATTCGCCAAAGGTCTCCAGAAGGTTGTCCACAACTGCAGACAGAGCGT
  CACACACGAGCCCCACATGCCTCTCTTGTCCATCTACTCACTGGCCCTGGAACAAGATTTGGAGTTTGGC
  CACGGCATGGTGCAGGCAGCGGGCACACTGCAGACCCAGACCTTCATGCAGCCCCTGACGCTGCGGCGGT
  TGGAGCATGAGAGGCGCAGGAAGGAGATCAAAGAATCTTGGCATCGAGCACAGAGGAAGCTGCAAGAGGC
  AGAGGCCAACCTGCGCAAGGCCAAACAGGGCTACAAACAACGCTGTGAAGACCACGACAAGGCCCGGCTC
  CAGGTGGCCAAGGCTGAGGAGGAACAACAGGGCACAGGCCCAGGCGCAGGGACTGCGGCCTCTAAGGCCC
  TGGACAAGAGGCGCAGACTGGAGGAAGAGGCCAAAAACAAGGCCGAGGAGGCCATGGCCACTTACCGCAC
  ATGCGTGGCAGACGCAAAGACACAGAAGCAGGAGCTGGAGGACACAAAGGTGACTGCGCTGCGGCAGATT
  CAGGAGGTCATCAGGCAGAGTGATCAGACCATTAAGTCGGCCACCATCTCCTACTACCAGCTGATGCACA
  TGCAGACAGCACCGCTGCCCGTGAACTTCCAGATGCTGTGCGAGAGCAGCAAACTATATGACCCGGGCCA
  ACAGTACGCATCTCACGTGCGTCAGCTGCAGAGAGGCGAGGAGCCGGACGTGCGCTACGACTTTGAGCCT
  TATGTCTCTAACAACTCCTGGTCCCCAATCATGCGTACACGGAAGGGCAGCTTCAACCCTGGTGATGCTT
  CAGGACCCGAAGCTGCTGGTAGTCCCCCCGAGGAAGGTGGCACCTCTGAGGCAGCACCCAACAAGGACCA
  CAGGGGGGGACGAGGTCACCAGGTTCATAAGTCCTGGCCCATCTCCATCTCAGACACCGAAGTTGGTCTG
  GACACCAGCTCAGGGGACTTGAAGAAGTTCGATCGAACATCTTCCAGTGGGACGATGTCATCCAGTGAGG
  AGCTGGGGGATCAGGAAGCTGGCTTGGTAGCTTCCGCCTTTGATTCAGCTGACCTCAATGGCATGGACCC
  CGAGTTACCTGTGGCCATGCCCAGTGGACCCTTCCGCCATGTGGGATTGTCCAAGGCAGCCCGCACACAC
  CGACTTCGCAAACTGCGCACGCCAGCCAAGTGCAGAGAGTGTAACAGCTATGTGTACTTCCAAGGAGCTG
  AATGTGAAGAGTGCTGTCTGGCTTGTCACAAAAAGTGTTTGGAGACCCTGGCCATCCAGTGTGGCCACAA
  GAAGCTTCAGGGCCGCCTGCAGCTCTTTGGACAAGACTTCAGCCAGGCAGCCCTCAGCACCCCTGATGGG
  GTGCCCTTCATTGTCAAAAAATGTGTCTGTGAGATTGAGCGGCGGGCACTGCACACCAAGGGGATCTACC
  GGGTCAATGGCGTGAAAACGCGTGTGGAGAAGCTGTGCCAGGCTTTTGAGAATGGCAAAGAGTTGGTGGA
  GTTGTCACAGGCCTCACCCCACGACATCAGCAACGTCCTAAAGCTATACCTGCGGCAGCTACCGGAGCCC
  CTTATCTCTTTTCGCTTCTACCATGAGCTGGTGGGACTAGCTAAGGACAGCCTAAAGGCCGAAGCCGAAG
  CTAAGGCAGCGAGCCGGGGTCGGCAGGGTGGGTCCGAGAGTGAGGCTGCAACCTTGGCTATGGTGGGCCG
  CCTGCGTGAGCTCATGCAGGATCTGCCAGCCGAAAACCGGGCCACACTCTTATACCTGCTGAAGCACTTG
  CGAAGGATCGTGGAAATGGAGCAGGATAACAAGATGACCCCTGGGAACCTGGGCATCGTTTTCGGGCCCA
  CACTGTTGCGGCCTCGACCCACTGACGCCACCGTGTCCCTCTCTTCTCTGGTGGATTATCCCCACCAGGC
  CCGAGTTATTGAGACTCTGATTGTCCACTACGGCCTGGTCTTTGAGGAGGAGCCAGAGGAGGCGCCTGGC
  AGCCAGGAGGGGGCATCCACCCAGTGTGGCCAGCTGGAGTCTGCTGAGGGCATTGTCTTCCCCCTGCAGG
  AGGAGGCAGAGGACGGAAGCCGAGAATCTCACGCGGCATCTAATGACTCGGACTCTGAGCTGGAAGACGC
  TTCTGACCCCCTTTCATCCTCGGACGCCAGTGCTCTACACCGCCTCAGTTTCCTGGAGCAGACCGAGGCA
  GGCCTGGAGGAAGGTCCCCAGAGCCATAGTGGCAGTGAGGAACAGCTGGAGGGTGAGGATGGAGCCCCGG
  GTCCGTGGCTGTGTCACTTCAACACCAACCAGTCCAACAACACATCACGGGCCCCTCTGCCCACCATGCG
  GCTCCGAGGAGGGCAGATCACAGGTGGCACCAGTCAGGAGCGGCAGCCACAGTTCGTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218590 representing NM_001142701
  Red=Cloning site Green=Tags(s)
  
  MFSRKKRELMKTPSISKKNRAGSPNPQSSSGELPRKDWTEAPGLEPPATSLSTVAKGTGTLKRPTSLSRH
  ASAAGFPLSGTATWTLGRGYRSPLSAASPAELPTEGAFPDGVEDISTLLADVARFAEGLEKLKECVLQDD
  LLEARRPLAHECLGEALRVMRQVISRYPLLNTVETLTAAGTLIAKVKAFHYECNNESDKREFEKALETIA
  VSFSCTVSEFLLGEVDSSTLLAVPPGDPSQSMENLYGAGTEGPPHNVEECEEGCLPPEEVDMLLQRCEGG
  VDAALQYAKDMARYMKDLISYLEKRTTLEMEFAKGLQKVVHNCRQSVTHEPHMPLLSIYSLALEQDLEFG
  HGMVQAAGTLQTQTFMQPLTLRRLEHERRRKEIKESWHRAQRKLQEAEANLRKAKQGYKQRCEDHDKARL
  QVAKAEEEQQGTGPGAGTAASKALDKRRRLEEEAKNKAEEAMATYRTCVADAKTQKQELEDTKVTALRQI
  QEVIRQSDQTIKSATISYYQLMHMQTAPLPVNFQMLCESSKLYDPGQQYASHVRQLQRGEEPDVRYDFEP
  YVSNNSWSPIMRTRKGSFNPGDASGPEAAGSPPEEGGTSEAAPNKDHRGGRGHQVHKSWPISISDTEVGL
  DTSSGDLKKFDRTSSSGTMSSSEELGDQEAGLVASAFDSADLNGMDPELPVAMPSGPFRHVGLSKAARTH
  RLRKLRTPAKCRECNSYVYFQGAECEECCLACHKKCLETLAIQCGHKKLQGRLQLFGQDFSQAALSTPDG
  VPFIVKKCVCEIERRALHTKGIYRVNGVKTRVEKLCQAFENGKELVELSQASPHDISNVLKLYLRQLPEP
  LISFRFYHELVGLAKDSLKAEAEAKAASRGRQGGSESEAATLAMVGRLRELMQDLPAENRATLLYLLKHL
  RRIVEMEQDNKMTPGNLGIVFGPTLLRPRPTDATVSLSSLVDYPHQARVIETLIVHYGLVFEEEPEEAPG
  SQEGASTQCGQLESAEGIVFPLQEEAEDGSRESHAASNDSDSELEDASDPLSSSDASALHRLSFLEQTEA
  GLEEGPQSHSGSEEQLEGEDGAPGPWLCHFNTNQSNNTSRAPLPTMRLRGGQITGGTSQERQPQFV
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142701
• ORF Size: 3348 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142701.1, NP_001136173.1
• RefSeq Size: 3928 bp
• RefSeq ORF: 3351 bp
• Locus ID: 70719
• UniProt ID: Q3TBD2
• Cytogenetics: 10 C1
• MW: 123.3 kDa
• Gene Summary: Contains a GTPase activator for the Rho-type GTPases (RhoGAP) domain that would be able to negatively regulate the actin cytoskeleton as well as cell spreading. However, also contains N-terminally a BAR-domin which is able to play an autoinhibitory effect on this RhoGAP activity.[UniProtKB/Swiss-Prot Function]