Nhsl1 (NM_173390) Mouse Tagged ORF Clone

CAT#: MR218416

Nhsl1 (Myc-DDK-tagged) - Mouse NHS-like 1 (Nhsl1), transcript variant 1

Product Images

Circular map for MR218416

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nhsl1
• Synonyms: 5730409E15Rik; A630035H13Rik; A730096F01; BC013565; D10Bwg0940e; mKIAA1357
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218416 representing NM_173390
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAAAGGACGGCTCCTCAGGTTCCTTCGGAATCAAGGCAAGCCCAGGCTCCCTCTCACGTGCTGTGA
  GTTGGATAAATTTCTCCTCCCTGTCCAGGCAGACAAAGAGGCTGTTCCGCAGTGATGGAGAGCTGTCCGT
  TTGTGGGCACCAGGTGGAAGCCGATGATGAGAACTGGATATACAGGACCCAGCCCAGAAAAGCGGTTTCC
  AACTTGGACGAGGAGAGTCGATGGACTGTCCACTACACGGCACCATGGCATCAGCAAGAGAATGTGTTCC
  TTCCCGCCACAAGACCCCCCTGTGTTGAGGACCTGCACCGCCAAGCCAAGCTCAACCTCAAATCAGTGCT
  GAGGGAATGTGACAAGCTGAGGCAAGATGGCTGCCGAAGTTCTCAGTACTATTCTCAGGGACCCACCTTT
  GCAGCCGGCTCCAGCCCCTGCGACGATTATCAAGATGAAGACACAGAAGCAGACCGAAAGTGCTCTCTCT
  CTTCATCTGAAGAAGAAAGATTTATCGGAATCAGGAGACCTAAAACGCCAACCTCAGGTGACTTCTCCGA
  CCTTCATACTCAGACAAACTGGACCAAGTCCCTGCCGCTGCCAACACCAGAAGAGAAGACGCGGCAGCAA
  GCCCAGACGGTCCAGGCTGACGTGGTTCCTATAAACATAACTGCATCAGCCACTGGCCAAGATGATGATG
  GTAGTGCCCATTCGCTGTACGTCCCAGACCACTACTCTACGTTAGGAAGGCTTGATAGTTATCGGTCCAC
  CGGGCAATGCTTGGAAACCAGGGACACCAGCTGTCAGACCGAAGACGTGAAGGTCATCCCACCATCAATG
  AGAAGGATCAGGGCTCACAAGGGGGTAGGCGTTGCTGCCCAGATGAGCCACCTCTCAGGTTCCTCAGGCA
  ACATGTCTGTGCTGAGTGACTCAGCCGGTGTTGTGTTCCCATCTCGCCTCAGTAACGATACTGGTTTCCA
  CAGTCTTCCACGTACCGGCCCGAGAGCAAGCACCTATTCACTGGAGGGAAGGATGGGTGCCCTGGGCTCT
  ACTGAAGATACGGATGACACTTCTCCCTACCAGGGGGGTAGCTTACAGGGGCATGAAAACTTTGCGCATT
  TAGGAGGTGCGTCGAGTACTGGGATGCTTTCGAGGCCCAAATCCCAGCAGTTGAGGTTTTTGGAGAGTCC
  AGCATGTGTGGTTTCACCTCATGCAGCCTACTCTACCAGTGTCATCCCAAATGCCACGCTACTGTCCTCT
  TCAGAGGTTATTGTCATTCACACTGCTCAGAGTGCAGGACAGCTGGACAGTAGAACACCCGGTTCATCTT
  CATATTCAAAAATAAAACCCAGAGACCGTCCCACACCCAGATGTTCTGTGAAAGATGACCATCAGTCCCC
  CCGTCATCACTGGAACGAGGGTCATCTCATTCATTCACGGGCTTTAGCCTCCTCTGTCCCTGGTGCCACC
  ACGCTGTTATCCCTCCATGATTCAGAGGTCTCTCTAAATGCCCCAGCAAACAGGGAAAATGGATCCCAAG
  CCATACTCTATCATTGTAGAAACAACCCAAGCTTTCCTGACCACCCTTCAGATGTGGATGGCAAGAGCGA
  GTGTAGTTATTCGGGGGACAGGGGATGTGGCAGTTCTGAGCCCTGGGAATATAAAACCTCCAGCAATGGG
  CGGGCATCCCCACTGAAGCCACACCTGGCCACTCCTGGTTGCTCTACTCCCACAAGTAACGTGAGCAGCT
  GCAGTTTGGACCAAACATCTCTCAAGGGTGATACCAGGTCCCTGTGTTCAGAGGACCATGATGGTTACTA
  TACTACCACTCACGAGGCTGGGAACCTGTACACTCTCAGTGATGGCTTGGGGAACCCCAGGCACAGCATG
  GTCAATGTTTTTGATGGAAGGGCTCAGAGAAGCCAAGGGGATCAGGCCGCTCACCAGGATAAAATCCTTT
  CGAGAAACATCTCTCTGAAGAAAGCAAAGAAACCACCCCTGCCACCATCCCGGACCGATTCTCTGCGAAG
  GATTCCCAAAAAGAACAACCAGACAAACGGTCAGGTGCTCAATGAGAGCCTGATCGCCTCGCTCCAGCAT
  TCACTGCAGCTAAGCCTCCCTGGCAAGGGCGGCAGCTCCCCTTCCCAGAGCCCCTGCAGTGACTTTGAGG
  AACCCTGGCTCCCCAGGTCCAGAAGCCAGAGCATTGTTAGCGAGGGTAGTAGCTTGACCTCCACCACCAC
  TCCCAACGTGTATTCTCTGTGTGGGGTCACTCCGTCCCAGAGCGACACAAGTAGCGTCAAGTCGGAATAC
  ACGGACCCTTGGGGTTACTACATTGACTACACGAGCTTGCAGGAAGATCCAGGGAACCCCACCGGGGGCT
  GCTCAGCCAACACTGAGGCAGCAACTGGAAACGGGCCAGTACGCCACATCCAGGAGGGGTCGAGAGTCCC
  AGTGCCCCAAGTGCCGGGCTGCTCAGTTAGACCAAAGATCGCCTCACCAGAGAAGTCACAGAGAGTCACC
  TCTCCATCCAGTGGGTATTCTAGCCAGTCGAATACACCCACAGCACTCACCCCTGTGCCTGTGTTTTTAA
  AATCAATGTCACCAGCAAATGGAAAGGGGAAGGCCAAGCCCAAGGTACCAGAAAGAAAATCGTCTCTGAT
  CTCTTCTATGTCCATCTCATCATCGTCCACTTCTCTCTCTTCTAATACTTCTACCGAAGGAAGTGGGACC
  ATGAAGAAACTGGATACAACCCTGGCCTCGGCCCTTGCCCCTCCCCCTCCTCCTCTTCCCCCTCTGCCTT
  CTCCGTGTCTGGCAGACAAGTCCCCTTTTCTTCCACCACCTCCTCCTCTAGCAGATTGCTCCGAGGGCTC
  TCCTCTGCCGCCCTCTCCCATGTTCCCCCCTCCACCACCAGAAGCCCTTGTTCCCTTCTGCTCCCCCACC
  GACGGGTGCCTTTCTCCTTCTCCCACAGCAGTTAGCCCCTCTCTTCCAAGATCCTTGCCTCCTGTGCCAG
  CACCTCCACCTTTCTTGCCCTCATCAGAACCCCCGCCCGCTCCGCCTCTGGACCCCAAACTCATGAAAGA
  AAACAGGCCCTTTTTCAAAAACTCTAGCCAGTCCGAATCCTCTAGGGAGGCCCTCAGGCGGCCTGCCAAC
  AAGGAGGAGGGCTGCCGACCCCCCATGCCCCTGATCACCACAGAAGCTTTGCAGATGGTGCAGTTGAGGC
  CAGTGAGGAAGAACTCAGGTGCCGGGGCAGTCCTGTTTTCTGAACCATCAGCTCAGGAACAACGAACTCC
  GACTGCTCCACAGTATCACTTAAAGCCATCTGCTTTCCTCAAATCCCGAAATAGCATAAATGAAATGGAG
  AGTGAAAGCCAGGCTGCCTCTGTGACGAGCTCGCTTCCGATGCCTGCCAAGAGCCAGAGTCAGGGTGACC
  ATGACAGTGCAGTAGAGCGTGGCGGCCTTCCGAGCTGCAGCGATGGAGCTCCAGGCCCGGGTCCCAGCCT
  CAGGACCACTCTGCTCCCAGACTCTTCACCCAGCAGGAAGCCACCCCCCATCTCTAAGAAGCCCAAACTG
  TTCCTGGTGGTACCACCTCCGCAGAGAGATTTCACAGCGGAGCCCACAGAGAACGGGAGCGAAGCTTTCC
  CAGGCGTGCCCAGCCCTACCAGGGCAGAGGGGGAAGCTGTGCGGAGCCAAGAGGAGAAATCCAGCCCAGC
  ATCCCGAGCTGGCTCTCATGCTACGGCCCCCACCCCCGGCAGTCCGGCTCTAGAACCGGGAACTGCAGGG
  TCCTTATCCTCTAGCATTGTGGAAGCCAATGTCCCCATGGTCCAACCTAATACATCACCGGGCCCCACAC
  AGGAAGAGTCAGGCGAGAACAGTGTGGATGGTGAGAGAAATGCGAAGAGCTGCCTGTCTCAACAGGGCAG
  AGAAGCTGGCTTGCTGGAACCCAACACAGCTGCTTCCTCTTCAGACCCCGTGGATGTATCTAAGGAAGAG
  GGAAGCGATGAGGTGCTGACTCCGACTAAACCCAGGACAACAGAAGACCTGTTTGCAGCCATTCACAGAT
  CTAAAAGGAAAGTCCTTGGCCGGAAAGACTCAGAAGACGATCACACTCGAAATCACTCTCCCTCCCCGCC
  TGTGACACCCACGAGTGCTGCCCCTAACCTGGCCTCTCCAAAGCAAGTAGGGTCTATCCAGAGAAGCATA
  AAAAAGAGCACTACCAGCAGTGACAACTTCAAAGCTCTGCTGCTAAAGAAGGGGAGTCGCTCGGACACCA
  GCGCTCGCATGTCAGCGGCTGAGATGCTCAAGTCTACGGACCCTCGCTTCCAGAGATCTAGGTCGGAGCC
  TTCGGCAGACAGCCCGGACAGCCCATCCAGCTGCTCCCCCAACAAGAACAGGAGAGCTCAGGAAGAGTGG
  GCCAAGAACGAAGGCCTGATGCCTCGGAGTCTGTCCTTTTCGGGCCCTCGGTACAGCCGCAGCAGGACGC
  CACCCTCTGCGGCCAGCAGCAGGTACAGCATGCGGAACCGCATCCAGAGCAGCCCCATGACCGTCATCTC
  CGAGGGAGAGGGAGAACCTGCTGAGCCAGCGGACAACAAAGCACGCAGGGCCCTGGATGCTACCAGGGTG
  TGCTCTCTGGACAGACTGACGGGTCAGGAGATGGACCAGGCCAGCCTCCTCTGTAGCGAGGAGCCTGCCT
  CTGTGGATGGGATAGGAAGGGCTGAGGGCAACGGTCCCTCGGAGCAGTGTGGGGGTACGGAACAGAAGAG
  T
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218416 representing NM_173390
  Red=Cloning site Green=Tags(s)
  
  MKKDGSSGSFGIKASPGSLSRAVSWINFSSLSRQTKRLFRSDGELSVCGHQVEADDENWIYRTQPRKAVS
  NLDEESRWTVHYTAPWHQQENVFLPATRPPCVEDLHRQAKLNLKSVLRECDKLRQDGCRSSQYYSQGPTF
  AAGSSPCDDYQDEDTEADRKCSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQ
  AQTVQADVVPINITASATGQDDDGSAHSLYVPDHYSTLGRLDSYRSTGQCLETRDTSCQTEDVKVIPPSM
  RRIRAHKGVGVAAQMSHLSGSSGNMSVLSDSAGVVFPSRLSNDTGFHSLPRTGPRASTYSLEGRMGALGS
  TEDTDDTSPYQGGSLQGHENFAHLGGASSTGMLSRPKSQQLRFLESPACVVSPHAAYSTSVIPNATLLSS
  SEVIVIHTAQSAGQLDSRTPGSSSYSKIKPRDRPTPRCSVKDDHQSPRHHWNEGHLIHSRALASSVPGAT
  TLLSLHDSEVSLNAPANRENGSQAILYHCRNNPSFPDHPSDVDGKSECSYSGDRGCGSSEPWEYKTSSNG
  RASPLKPHLATPGCSTPTSNVSSCSLDQTSLKGDTRSLCSEDHDGYYTTTHEAGNLYTLSDGLGNPRHSM
  VNVFDGRAQRSQGDQAAHQDKILSRNISLKKAKKPPLPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQH
  SLQLSLPGKGGSSPSQSPCSDFEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGVTPSQSDTSSVKSEY
  TDPWGYYIDYTSLQEDPGNPTGGCSANTEAATGNGPVRHIQEGSRVPVPQVPGCSVRPKIASPEKSQRVT
  SPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSMSISSSSTSLSSNTSTEGSGT
  MKKLDTTLASALAPPPPPLPPLPSPCLADKSPFLPPPPPLADCSEGSPLPPSPMFPPPPPEALVPFCSPT
  DGCLSPSPTAVSPSLPRSLPPVPAPPPFLPSSEPPPAPPLDPKLMKENRPFFKNSSQSESSREALRRPAN
  KEEGCRPPMPLITTEALQMVQLRPVRKNSGAGAVLFSEPSAQEQRTPTAPQYHLKPSAFLKSRNSINEME
  SESQAASVTSSLPMPAKSQSQGDHDSAVERGGLPSCSDGAPGPGPSLRTTLLPDSSPSRKPPPISKKPKL
  FLVVPPPQRDFTAEPTENGSEAFPGVPSPTRAEGEAVRSQEEKSSPASRAGSHATAPTPGSPALEPGTAG
  SLSSSIVEANVPMVQPNTSPGPTQEESGENSVDGERNAKSCLSQQGREAGLLEPNTAASSSDPVDVSKEE
  GSDEVLTPTKPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTSAAPNLASPKQVGSIQRSI
  KKSTTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSADSPDSPSSCSPNKNRRAQEEW
  AKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKARRALDATRV
  CSLDRLTGQEMDQASLLCSEEPASVDGIGRAEGNGPSEQCGGTEQKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173390
• ORF Size: 4761 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173390.3, NP_775566.3
• RefSeq Size: 7335 bp
• RefSeq ORF: 4764 bp
• Locus ID: 215819
• UniProt ID: Q8CAF4
• Cytogenetics: 10 7.65 cM
• MW: 169.9 kDa