Eif5b (NM_198303) Mouse Tagged ORF Clone

CAT#: MR218074

Eif5b (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 5B (Eif5b)

Product Images

Circular map for MR218074

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eif5b
• Synonyms: A030003E17Rik; BC018347; IF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218074 representing NM_198303
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAAGAAACAGAAAAACAAGAGCGAAGACAGTACCAAGGATGATACTGATTTGGGCGCCTTGGCTG
  CAGAAATAGAAGGAGCTGGTGCTGCCAAGGAGCAGGAGCCTCAGAAGTCAAAGGGAAAGAAGAAAAAGGA
  GAAAAAGAAACAAGACTTTGATGAGAACGACATTCTGAGAGAGCTGGAGGAGTTGTCTCTGGAAGCCCAA
  GGCATCAGAGCTGACAGAGACGCCGCTGCAGTCAAGCCTACAGAAAACAATGAAGAAGAATCTGCCTCAA
  AACAAGATAAAAAGAAGAAGGGACAAAAAGGCAAGAAAACAAGTTTTGATGAGAATGATAGTGAAGAATT
  GGAAGATAAAGATTCAAAGTCAAAAAAGACTGCCAGACCTAACTCAGAGGCACCCCTTTCTGGGAGTGAG
  GATGCTGATGATTCTAATAAACTTTCTAAAAAAGGTAAAAAAGCCCAGAAATCCACGAAAAAACGGGACG
  GGTCAGAAGAGGATGAAGACAATAGCAAAAGAAGTAAAGAACGTTCGAGAGTAAACTCCTCTGGTGAAAG
  TGGTGGTGAGTCCGATGAATTTTTGCAGTCCAGAAAAGGACAGAAAAAAAATCAGAAGAACAAGTCCGTT
  CCTACCGTAGACAGTGGGAATGAGGACGATGACTCTTCCTTCAAAATTAAGACGGTGGCCCAGAAGAAGG
  CAGAAAAGAAAGAGCGCGAGAAGAAAAAGAGAGATGAAGAGAAAGCGAAGTTGCGGAAGATGAAGGAGAA
  AGAAGAGCTAGAGAAGGGCAAGAAGGAGCAGAGTAAACAGAGGGAGCCTCAGAAGAGACCTGAGGAGGAG
  GTGTTGACACTCAGAGGGACTCCTGACACAGGAGCTGCCTCGGAAGAGAAAGGAGACACTGCTGCAGCTC
  TGGAAGACGACAATGAAGGAGACAAAAAGAAAAAAGATAAGAAGAAAAAGAAAACAGAGAAAGATGAGAA
  AGAAAAAGAGAAGAAGAAAGGCCCTAGTAAATCTACTGTTAAAGCTATCCAGGAAGCTTTGGCTAAGCTC
  AAAGAGGAGGAGGAGAGACAGAAGAGAGAGGAGGAAGAAAGGATTAAGCGGCTTGAGGAGCTTGAAGCCA
  AACGAAAAGAAGAGGAACGATTAGAACAAGAAAAAAGAGAAAGGAAAAAGCAAAAAGAAAAAGAAAGAAA
  AGAACGGTTAAAAAAAGAAGGAAAGCTGTTAACTAAGTCCCAGAGAGAAGCCAGAGCCAGAGCCGAAGTG
  ACCCTTAGACACCTCCAAGCTCAGGGTGTTGAAGTGCCATCAAAAGACTCGTTGCCAAAGAAAAGGCCAG
  TTTATGAAGATAAGAAGAAGAAGAAAACACCACAGCAGCTAGAGAGTAAAGAAGTATCTGAAACTTTGGA
  AATAAGTGCTCCTGTGGAAGCTGTGGACCAAGGAGGACCAGAAAAGGAAGAGACGCCACCTTCTGTTGAG
  CCAGAGGAAGAGGAAGACACCGAAGATGCTGGCTTAGATGACTGGGAGGCTATGGCCAGTGATGAGGAGA
  GAGAAAAAGAAGGAAATATGATTCACATAGAAGTAGAAGAAAACCCTGAGGAAGAAGAAGAGGAAGAAGA
  AGAGGAGGAGGAAGAAGAGAGTGAAGATGAAGAAGAGGAAGGGGACAGTGAAGGCAGCGATGGGGACGAG
  GAGGACTGCAAGCTGTCGGATGAGAAGGACTCAGGGAAGGCTGGAGACACGAAGCCCAGCAAAGACGCTA
  GCTCAGACTCCGAGTACGACTCCGACGACGATCGGACTAAAGAAGAGCGGGCGTACGACAAAGCGAAACG
  GAGGATTGAGAAACGACGACTTGAACATGGTAAAAATGTAAATACAGAAAAGCTGAGAGCCCCTATCATC
  TGTGTGCTTGGACATGTAGACACAGGAAAGACAAAAATTCTAGATAAGCTCCGTCACACACATGTGCAAG
  ATGGCGAAGCAGGTGGTATTACACAGCAGATTGGTGCCACAAATGTTCCTCTTGAAGCTATTAATGAACA
  AACTAAGATGATTAAAAATTTTGACAGAGAGAATGTCCGAATCCCAGGGATGCTGATTATTGATACTCCT
  GGACACGAGTCTTTCAGTAACCTGAGAAACAGAGGAAGCTCTCTTTGTGACATTGCCATTTTAGTTGTTG
  ACATTATGCATGGTTTAGAGCCCCAGACAATTGAATCAATCAACATTCTCAAATCTAAAAAGTGCCCCTT
  CATTGTTGCACTTAATAAGATTGACAGGTTATATGATTGGAAGAAGAGTCCTGATTCTGATGTGGCTGTT
  ACTTTAAAAAAGCAGAAGAAGAATACAAAGGATGAATTTGAGGAGCGTGCAAAGGCCATTATTGTAGAAT
  TTGCACAGCAGGGTTTGAATGCTGCTTTGTTTTATGAGAATAAAGATCCCCGCACTTTCGTGTCCTTGGT
  ACCTACCTCTGCACATACTGGTGATGGCATGGGAAGTCTCATCTACCTTCTTGTGGAGTTAACTCAAACC
  ATGTTGAGCAAGAGGCTTGCACACTGTGAAGAGCTGAGAGCACAAGTCATGGAGGTTAAAGCTCTCCCAG
  GAATGGGCACCACAATAGATGTCATATTGATCAATGGACGTTTGAAGGAAGGGGATACAATCATTGTCCC
  TGGAGTAGAAGGGCCCATTGTCACTCAGATTCGAGGCCTCCTTTTACCTCCTCCTATGAAGGAATTACGT
  GTGAAGAACCAATATGAAAAGCATAAAGAAGTGGAAGCGGCCCAGGGAGTAAAGATTCTTGGGAAAGACC
  TTGAAAAAACCCTGGCTGGCTTACCTCTCCTAGTGGCCTACAAGGATGATGAAATCCCAGTTCTCAAAGA
  TGAATTGATCCATGAGTTAAAGCAGACACTAAATGCTATCAAACTAGAAGAAAAAGGAGTTTATGTCCAG
  GCATCTACACTGGGCTCTTTGGAAGCTCTTCTTGAATTTCTGAAAACATCAGAGGTGCCATATGCAGGAA
  TTAACATTGGCCCAGTCCATAAAAAGGACGTTATGAAGGCTTCAGTGATGTTGGAACATGATCCCCAGTA
  TGCAGTAATTTTGGCCTTTGATGTAAGAATTGAACGGGACGCACAAGAGATGGCTGATAGTTTGGGAGTT
  AGAATCTTTAGTGCAGAAATTATTTATCACTTATTTGATGCCTTTACAAAATACAGACAGGACTACAAAA
  AACAAAAACAAGAAGAATTTAAGCACATAGCAGTATTTCCCTGCAAGATGAAAATCCTCCCTCAATACAT
  TTTCAATTCTCGAGATCCAATAGTAATAGGGGTCACTGTGGAGGCTGGTCAAGTGAAACAAGGAACACCT
  ATGTGCGTTCCAAGCAAAAATTTTGTTGACATTGGGATAGTAACAAGCATTGAAATAAACCACAAGCAGG
  TGGATGTTGCAAAAAAAGGACAAGAGGTCTGTGTCAAAATAGAACCTATCCCTGGGGAATCTCCCAAAAT
  GTTTGGGAGACATTTTGAAGCTACAGATATACTTGTCAGCAAGATCAGCCGGCAGTCCATCGATGCACTC
  AAAGACTGGTTCAGAGACGAAATGCAGAAGAGTGACTGGCAGCTTATTGTGGAGCTGAAGAAAGTATTTG
  AAATCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218074 representing NM_198303
  Red=Cloning site Green=Tags(s)
  
  MGKKQKNKSEDSTKDDTDLGALAAEIEGAGAAKEQEPQKSKGKKKKEKKKQDFDENDILRELEELSLEAQ
  GIRADRDAAAVKPTENNEEESASKQDKKKKGQKGKKTSFDENDSEELEDKDSKSKKTARPNSEAPLSGSE
  DADDSNKLSKKGKKAQKSTKKRDGSEEDEDNSKRSKERSRVNSSGESGGESDEFLQSRKGQKKNQKNKSV
  PTVDSGNEDDDSSFKIKTVAQKKAEKKEREKKKRDEEKAKLRKMKEKEELEKGKKEQSKQREPQKRPEEE
  VLTLRGTPDTGAASEEKGDTAAALEDDNEGDKKKKDKKKKKTEKDEKEKEKKKGPSKSTVKAIQEALAKL
  KEEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEV
  TLRHLQAQGVEVPSKDSLPKKRPVYEDKKKKKTPQQLESKEVSETLEISAPVEAVDQGGPEKEETPPSVE
  PEEEEDTEDAGLDDWEAMASDEEREKEGNMIHIEVEENPEEEEEEEEEEEEEESEDEEEEGDSEGSDGDE
  EDCKLSDEKDSGKAGDTKPSKDASSDSEYDSDDDRTKEERAYDKAKRRIEKRRLEHGKNVNTEKLRAPII
  CVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTP
  GHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINILKSKKCPFIVALNKIDRLYDWKKSPDSDVAV
  TLKKQKKNTKDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQT
  MLSKRLAHCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELR
  VKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKDDEIPVLKDELIHELKQTLNAIKLEEKGVYVQ
  ASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVILAFDVRIERDAQEMADSLGV
  RIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPCKMKILPQYIFNSRDPIVIGVTVEAGQVKQGTP
  MCVPSKNFVDIGIVTSIEINHKQVDVAKKGQEVCVKIEPIPGESPKMFGRHFEATDILVSKISRQSIDAL
  KDWFRDEMQKSDWQLIVELKKVFEII
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198303
• ORF Size: 3648 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198303.2, NP_938045.2
• RefSeq Size: 7797 bp
• RefSeq ORF: 3651 bp
• Locus ID: 226982
• UniProt ID: Q05D44
• Cytogenetics: 1 B
• MW: 137.6 kDa
• Gene Summary: Plays a role in translation initiation. Translational GTPase that catalyzes the joining of the 40S and 60S subunits to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. GTP binding and hydrolysis induces conformational changes in the enzyme that renders it active for productive interactions with the ribosome. The release of the enzyme after formation of the initiation complex is a prerequisite to form elongation-competent ribosomes.[UniProtKB/Swiss-Prot Function]