Gprasp2 (NM_001163015) Mouse Tagged ORF Clone

CAT#: MR217980

Gprasp2 (Myc-DDK-tagged) - Mouse G protein-coupled receptor associated sorting protein 2 (Gprasp2), transcript variant 1

Product Images

Circular map for MR217980

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gprasp2
• Synonyms: 5330440H13Rik; GASP-2; GASP2; Prpl5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217980 representing NM_001163015
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGGGGCAGAAGTTGAGACCGGTTCTCAGGCCAAGCCTGATAAGAAGCCTCAGGAAGAGGTCGCAG
  GTGGGGCTGAGAGAGAAAGTGAAGCCCCTCTGGTGGTCAGACCCAAAGTCAGGCCTCAGGCTCCAGCAAC
  AAGTGGTGCTAGGCCCAAAACTGAAACTAAATCTTCCTCTCGGGCAAGGCCTAAAACTGAAACCCAGTCA
  GTGTCTGGAACAAGACACCGAATGAGTGGAGCAAGGCCGAGATCTGAGGCTCAATTAATGTCTGGGGCCA
  GGCCTAAAACCGATGCCAGGGCAGTAGGTGGTGCTCGCCCTAAGACTGAGGCCAAGCCAATCCCAGGAGC
  AAGGCCTAAGGGTGATGCCCAGGCTTGGGCCCACAGTGAGTTTGGAGCCGAGGCAATGCCACGTGCTGAG
  AGAGCACACTTATCTAATTCTGTCACATGGCCACCAGTCAATGTTGGGTCTGCAACTGTTACTAAATCTA
  AGAGTCTGTCTATGAATACAGAACTGGCCAGTATGGGTAGTGAAATCTTTTCTGGCACCCAGGGTCAGCC
  TGGAATCGAGCCCTGGTTTGGACCGAGAGAAGAGGCTAATATGGGTTCTTGGTGCTATCCCAGGCCCAGA
  GCCAGAGAGGAGACCTCTAATGAGTCTGCAGATGAAAACTCTACAATGTCTTCTTTCTGGACTAGAGAGG
  AGACTAGTATCAGATCATGGCCCAGGGAAGAGGTGAACACTAGGTCCAGGCACAGGGCTAAACATCAGAC
  TAATGCCAGGTCGAAGCCCAGATCCAAACAAGATCCCTATATTGATTCTTTGTCTGGGTCCGAGGATGAG
  GCCAGTAACCCATTCTGCTTTTGGGCTGGAGAAAATACTAATGACATGTTTAGGGCCAGAGGCAGGGATG
  AGGCGAATGCCAGACCCAAGATCAGGACAAAGAGAGAGGACTATTTTGAAGATGAAGATGAGATCTATAA
  AGAGTCCTGGCTTTTGCCTGGGGAAGAGGGTAATAGATTTAGGCGCCGAGACAAGGAAGAGCCTAATAAG
  ACCTTGAAAAATGAGAACGAAAAAGATGTTAAAAATGATGAAACAGTCGAACAAGAGTCCAGGCTTGAGG
  AGGAAGTCATTATTGGGTCCTGGTTCTGGGCAGAACAGGAGACTAATGTGGAGGCTGGAGCTTCAGCAAT
  CTGTGACGCTGAACCAGGGGCTGAAGAGGGGGCCATTGGTGGATCCCTGTTCTGGACCGAAGAAAAGCCT
  AGTTTGGGGGCTGTGGCCAGAGATGAGGTCAGGCCTGAGTCTGAAGAAGAGGCACTATTTGGGTCCTGGT
  TTTGGGATAGGGATGAGGCCTGCTTTGACCCAAATCCTACTCCTGTGTACACAGCTAAGAGCAGGTACAG
  AGATCCAGAAGAGGATCTTAATTTAGCATCCAGGCCCAAAACCTGGGACGAGGTTACCATCGAATTCAAA
  CCTCCTTGCCATGGGCTTGGGTTCCCTTCCCCAAGACCCTTTATCATTCCTGAAGGGGCTTCTGGAAATA
  GTGAGGAAAAAGCCAAGAATGCAGAGCTTGGCGCAGAGGGGGAAGAGCAGGACTCTGTGGCTCAGCGTGA
  TCTTCCTGAACCCGAGTTTCCATTTCAGTATGATCCCTCTTACCGCTCAGTCCAGGAAATTCGCGAGCAT
  CTTAAGGCCAGGGAAAGTGCACAGCCAGAGAATTGGTCTTGCACCTGCATCCAGTGTGAGCTTAGAATTA
  GTTCTGCAGAATTTGAGGAGCTTCTTTTACTGATGGACAGGATTCGTGATCCTTTTATCCATGAGATAGC
  TAAGATTGCAATGGGCATGAGAACTGCTTCTCAGTTTACCCGGGATTTCATTCGCGATTCGGGTGTTGTC
  TCACTGATTGAAGCCTTGATGAATTATCCTTCCTCCCGAGTGAGGACTAATTTTTTGGAAAACATGGTTC
  ACATGGCTCCCCCTTATCCAAATCTAAACATGATTGAGACATTTATTTGTCAAGTGTGTGAGGAGACACT
  TTCACATAGCGTGAATTCCCCTGAACAGCTAACTGGAATGAGGATGCTTAGACATCTCACTATAACTACT
  GACTATCACGTACTGATTGCCAATTACGTGTCTGGGTTTCTCGCCTTATTAACCACAGGCGATGCAAGAA
  CCAAGTTTCATGTTCTGAAAATGCTGTTGAACTTGTCTGACAATCCGATGGTGGCAAAAAAACTATTCAG
  TGCCAAAGCTCTATCAATATTTGTGGGTCTCTTTAACATAGAGGAGACTAATGATAACATTCAGATTGTT
  ATTAAAATGTTTCAGAACATCAGTAATATTGTAAAAAGTGGAGCGATGTCTTTACTTGATGATGATTTCA
  GTCTTGAGCCGCTTGTTTCTGCATTTCATGAATTTGAAGAGCTAGCTAAACAGCTGCAGATCCAAATAGA
  CAATCAAAATGATCCCGAGGAGGGACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217980 representing NM_001163015
  Red=Cloning site Green=Tags(s)
  
  MTGAEVETGSQAKPDKKPQEEVAGGAERESEAPLVVRPKVRPQAPATSGARPKTETKSSSRARPKTETQS
  VSGTRHRMSGARPRSEAQLMSGARPKTDARAVGGARPKTEAKPIPGARPKGDAQAWAHSEFGAEAMPRAE
  RAHLSNSVTWPPVNVGSATVTKSKSLSMNTELASMGSEIFSGTQGQPGIEPWFGPREEANMGSWCYPRPR
  AREETSNESADENSTMSSFWTREETSIRSWPREEVNTRSRHRAKHQTNARSKPRSKQDPYIDSLSGSEDE
  ASNPFCFWAGENTNDMFRARGRDEANARPKIRTKREDYFEDEDEIYKESWLLPGEEGNRFRRRDKEEPNK
  TLKNENEKDVKNDETVEQESRLEEEVIIGSWFWAEQETNVEAGASAICDAEPGAEEGAIGGSLFWTEEKP
  SLGAVARDEVRPESEEEALFGSWFWDRDEACFDPNPTPVYTAKSRYRDPEEDLNLASRPKTWDEVTIEFK
  PPCHGLGFPSPRPFIIPEGASGNSEEKAKNAELGAEGEEQDSVAQRDLPEPEFPFQYDPSYRSVQEIREH
  LKARESAQPENWSCTCIQCELRISSAEFEELLLLMDRIRDPFIHEIAKIAMGMRTASQFTRDFIRDSGVV
  SLIEALMNYPSSRVRTNFLENMVHMAPPYPNLNMIETFICQVCEETLSHSVNSPEQLTGMRMLRHLTITT
  DYHVLIANYVSGFLALLTTGDARTKFHVLKMLLNLSDNPMVAKKLFSAKALSIFVGLFNIEETNDNIQIV
  IKMFQNISNIVKSGAMSLLDDDFSLEPLVSAFHEFEELAKQLQIQIDNQNDPEEGQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163015
• ORF Size: 2478 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163015.1, NP_001156487.1
• RefSeq Size: 3814 bp
• RefSeq ORF: 2481 bp
• Locus ID: 245607
• UniProt ID: Q8BUY8
• Cytogenetics: X 57.3 cM
• MW: 92.8 kDa
• Gene Summary: May play a role in regulation of a variety of G-protein coupled receptors.[UniProtKB/Swiss-Prot Function]