Epb41l4b (NM_019427) Mouse Tagged ORF Clone

CAT#: MR217939

Epb4.1l4b (Myc-DDK-tagged) - Mouse erythrocyte protein band 4.1-like 4b (Epb4.1l4b)

Product Images

Circular map for MR217939

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Epb41l4b
• Synonyms: 6430543G08Rik; AA589614; AU021054; BB007528; D4Ertd346e; Ehm2; Epb4.1l4b; Lulu2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217939 representing NM_019427
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGGTTCCTGCGCCGGACCTTTGGCCGCCGCTCCATGCAGCGCTACGCGCGGGGCGCGGCGGGGC
  GCGGGGCCGCCGGGCTGGGGGACGAGCGCGACGGGGGGCCGCGAGGGGGCCCGGCTGCCGCCGCCAGCTC
  CTCGGTGCTGCCCGCCGCGCCCGGGGGCAGCGTATTCCCGGCCGGCGGCGGGCCCCTGCTCACCGGCGGC
  GCGGCTGTGCACATCTCCGCCTCGGGAGCTGCCAAGGCCACCCTTTACTGCCGTGTCTTCCTGCTCGACG
  GAACCGAAGTGAGCGTGGACCTGCCGAAACATGCCAAAGGCCAGGATTTGTTCGACCAGATTGTGTACCA
  CTTGGACCTGGTGGAAACAGATTATTTTGGTCTGCAGTTCCTCGACTCTGCCCAGGTCACGCACTGGCTG
  GATCACGCGAAACCCATAAAGAAACAGATGAAAGTGGGACCTGCCTATGCCTTGCACTTTCGAGTCAAAT
  ACTATTCTTCAGAACCAAACAACCTTCGAGAGGAATTTACAAGGTACCTGTTTGTTTTACAACTCAGGCA
  TGACATTCTTTCTGGAAAATTGAAGTGCCCCTATGAAACGGCCGTGGAACTAGCTGCCCTCTGTCTGCAA
  GCGGAGCTAGGAGAGTGTGAGCTCCCGGAACACACGCCCGAGCTTGTGTCTGAGTTTCGGTTCATCCCAA
  ATCAGACAGAAGCCATGGAGTTCGATATCTTCCAGAGATGGAAAGAGTACAGGGGAAAGAGCCCTGCCCA
  GGCGGAACTCTCCTATCTGAACAAAGCGAAGTGGCTGGAAATGTATGGAGTGGACATGCATGTTGTCAGG
  GGAAGAGATGGCTGTGAATATTCTCTTGGACTGACCCCGACAGGCATATTAATCTTTGAAGGAGCTAACA
  AAATAGGCTTATTCTTTTGGCCTAAGATCACCAAAATGGACTTTAAAAAGAGTAAACTGACCCTCGTAGT
  AGTGGAAGATGATGACCAGGGGCGTGAGCAAGAGCACACCTTCGTGTTCAGGCTGGACAGTGCGCGTACC
  TGCAAGCACTTGTGGAAGTGTGCAGTGGAGCACCATGCCTTCTTCCGTCTGCGCACACCCAGCAACAGCA
  AGTCCGCCAGATCTGACTTCATCCGGCTGGGTTCCCGCTTCCGGTTCAGTGGACGGACGGAATATCAAGC
  TACACACGGCTCCAGATTGCGAAGAACCAGCACCTTTGAGAGGAAGCCTAGCAAACGTTACCCGTCCCGG
  CGACATTCCACATTCAAAGCGAGCAACCCAGTAATTGCTGCTCAGCTCTGCTCTAAAGCAAATCCTGAAG
  TCCACAATTACCAGCCTCAGTACCATCCTGACGTCCACCCCAGCCAGCCCCGGTGGCGCCCTCACTCTCC
  AAATGTCAGTAACCATTCCATCTGCAAACAAAACAAGCCATGTTTTCAGGATGACAGGCCACACTGGAAA
  ACCTCAGCCAGTGGAGATGGCAGCCACTTTGACTATGTCCAGGACCAGAACCAGAGGAACTTAGGAGGGG
  CGTACAGTGTCACGTACCGGGACAAACTCATGACCGCACTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217939 representing NM_019427
  Red=Cloning site Green=Tags(s)
  
  MLRFLRRTFGRRSMQRYARGAAGRGAAGLGDERDGGPRGGPAAAASSSVLPAAPGGSVFPAGGGPLLTGG
  AAVHISASGAAKATLYCRVFLLDGTEVSVDLPKHAKGQDLFDQIVYHLDLVETDYFGLQFLDSAQVTHWL
  DHAKPIKKQMKVGPAYALHFRVKYYSSEPNNLREEFTRYLFVLQLRHDILSGKLKCPYETAVELAALCLQ
  AELGECELPEHTPELVSEFRFIPNQTEAMEFDIFQRWKEYRGKSPAQAELSYLNKAKWLEMYGVDMHVVR
  GRDGCEYSLGLTPTGILIFEGANKIGLFFWPKITKMDFKKSKLTLVVVEDDDQGREQEHTFVFRLDSART
  CKHLWKCAVEHHAFFRLRTPSNSKSARSDFIRLGSRFRFSGRTEYQATHGSRLRRTSTFERKPSKRYPSR
  RHSTFKASNPVIAAQLCSKANPEVHNYQPQYHPDVHPSQPRWRPHSPNVSNHSICKQNKPCFQDDRPHWK
  TSASGDGSHFDYVQDQNQRNLGGAYSVTYRDKLMTAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019427
• ORF Size: 1581 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019427.2, NP_062300.2
• RefSeq Size: 2966 bp
• RefSeq ORF: 1584 bp
• Locus ID: 54357
• UniProt ID: Q9JMC8
• Cytogenetics: 4 31.66 cM
• MW: 59.6 kDa
• Gene Summary: Up-regulates the activity of the Rho guanine nucleotide exchange factor ARHGEF18 (PubMed:22006950). Involved in the regulation of the circumferential actomyosin belt in epithelial cells (PubMed:22006950). Promotes cellular adhesion, migration and motility in vitro and may play a role in wound healing (By similarity). May have a role in mediating cytoskeletal changes associated with steroid-induced cell differentiation (By similarity).[UniProtKB/Swiss-Prot Function]