Senp7 (NM_001003971) Mouse Tagged ORF Clone

CAT#: MR217815

Senp7 (Myc-DDK-tagged) - Mouse SUMO1/sentrin specific peptidase 7 (Senp7), transcript variant 2

Product Images

Circular map for MR217815

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Senp7
• Synonyms: 2410152H17Rik; 2810413I22Rik; 2900036C23Rik; 6030449K19Rik; AI790676; mKIAA1707
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217815 representing NM_001003971
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGGGCCAGGCCTGGGCGTCGGCGAGCTTCATCAGAGATTGTTACAGAGGGAAAACGGAAAAAGT
  CATCACCAGCTGATTTACAGAAGTGGACAAGAAGCCTAAGAAATAAAGTCATCTGTCTAGACCACAAAAA
  ACCAAAAGCTGCCCGTGGGTGTCCTCCTAAGGGATTACCAAAAAGGCATCTCAGAGTTATGTTGACGAAT
  GTTCTATGGACGGACTTAGGACGAGAATTCAGAAAGACCCTGCCTAGAAAGGATGCTAATTTATGTGCTC
  CCAGCAAGGTGCAATCAGACTCATTGCCTTCGACATCTGTTGACAGCATAGAGACATGTCAAAGATTAGA
  TCCTCTTCACCAAAGCCTTAATTTATCCGAAAGGACACCCAGAGTTATACTGACGGATATCCGGCAAACA
  GAATTAGGAAGAAAATATTTAAAGATCCCACCTGTAACTGAGGCCAGTTTGAGTGATACAGCCAACCTGA
  AATCAGAGCAACTTTCTTCATCATCTGATGGCAGCTTAGAGTCTTGTCAGAGTGTAAATCATCACAAGAG
  CTTTTTATCTGAAAGTGGTCCCAAACCAAGTAGGACAGGTGACGTTCCTGCAAAGGAGGCTGCATGTGGG
  GGACAGAAGCAGGGTGATGATGGAGGAGTCACTCCTGAGATGGCTGCTCCTCATCCTAAAGACTTTAATA
  CTGGAAATAAAGGTTGTGATTATCTTGAAGAGGGGACCAGCAACAAGAATACTTCATATTCTTATTCGGA
  AATGGACCATACTCCAGTTTCCAGGAAGAGAAAGAAAAGGGGTAGAAGTAATTTTCATGATTCTCATAAT
  TCTAAGACTTCTTTGGATAAGCCAACAGAACATACAAAAGAAGAAGAAAATGACTCTTCAGTCTCCCGTA
  AGCTTGAAGAGTCCGGTGAAGACTCTCACCAGGACCCAGCACCACCCGAAGGACTCGCGCCAGAATCTTT
  AGAGAGCGAGGCCACTAACCTGCGCTCATTCGCTGCTGGTCAGGAGCCAGATGCTAGCGCTGCCTCTGGA
  AGGGCCTCTTCACCCAACAAAAGCTTGGAGAGCTCTGCTTCTAGTGAAGTGTCGGAGAATTCTTCCGTGG
  CTGTGAAGGGTGAGGCGTTGACTCTAAAAGAAGCATCCCCTCCGGGTGGAAGCAGTGAAGAGAGCCAGTT
  GTTGATCTCAGCTGAGCCAATTGTAGTTTCCAGTGATGAAGAAGGACCGGTTGAACATAAGAATTCAGTA
  ATTCTAAAATTACAGCCTCCTCATGAGATTATGAGTGAAAATCAGGGCACTTCTGACCCACAGTTGTCAG
  AGCTGACACTCGGTGCATGTGAATCTGTACAGGTAACATCTGAATTATTTCCATACAATCCTGATGTAGA
  AAACATTTCCTGTATTAAATCTAATAGTGAAATGGATCTGAAATTGGATTTCATATTTACATGTGTGTAT
  ATTGGTAAAATAAAAGGAACTCCAAAAGGTTGTGTCACGTTCACAAAGAAGTATATTAAAATCCCGTTTC
  AAGTGTCCACAAATGAGATTTCCCTGACAGTGGACACCGCACGTTTAAAGAGGTTCGGGTTATGGGAAAG
  CAAGGATGAGGACCACAGCAAGAGGAGCCACGCTATCCTTTTCCTCTGGCTTTCCTCAGACTACCTTCAA
  GACATTCAGACCCAGCTAGAAAACCCCATGCTAAGCCAGCAATCAAAAGCCAATGAATTTATTTTCCTTG
  AGTTAAACAGTTCCATTTCACAAAGAGAAGAATTGAAATTGAAAGATATTATGATGGAAATCAGTACAAA
  AAATGGAAATTTACACCTGTCTTGTCCATTGCCCTGGGTCCAGGCACTTCCTTTGTTTCAGGATCTCTCT
  CCACAAGAAATCTCTTTTCTTCACTATTACTATGCTTCAGCTTCTGCTCTCCCCACTGCTGCTGGGGCAG
  ACATGAAGAAGAAGTCAGTGTCTCAGCCCTCAAATTCAGACACCATCAAGCCTACTTACACCTTCCTACA
  TAAACAAAGTAGTGGTTGCTACTCGCTCTCTATTACTTCAAGTCCAGAGGAGGAATGGCAGGAAGTCAGG
  AACACGGGACCCGTTCAGAAGTTGATTGTTTATCCTCCACCACCTACTAAGGGAGGCTTGGGAGTAACTA
  ATGAAGATCTAGAGTGTTTAGAAGAAGGAGAATTTCTTAACGATGTAATCATTGACTTTTACCTTAAGTA
  TCTTCTATTGGAGAAGGCATCAGATGAACTTGTTGAACGAAGTCACATTTTTAGTAGCTTTTTCTATAAA
  TGCTTGACAAGAAAGGAAAATAATTTAACAGAAGATAATCCAGATCTATCAGTGGCTCAGAGGAGACACC
  GAAGAGTGAGAACATGGACTCGTCATATAAACATTTTTAATAAAGACTACATCTTTGTACCTGTTAATGA
  ATCGTCTCACTGGTATCTTGCAGTCATTTGTTTCCCATGGCTAGAAGAGGCTGTGTATGAAGATTGTCCA
  CAAACTGTGTCCCAACAGTTCCAGGGTCAGCAGTCTCAGCATGACCACAAAATGACAGATAATGATCCAC
  ATACCACTTCAACTGTGTCCACGAGTGCAGAGGATTCCCAAAGTACCGAGGTGAATATGTCAGTACCAAA
  GAAAATGTGTAAGCGGCCATGTATTCTCATACTAGACTCCCTGAAAGCTGCTTCTATACAAAACACAGTT
  CAGAACTTACGAGAGTATTTGGAGGTAGAGTGGGAGGTGAAACGAAAAACGCACCGTGAATTCAGTAAAA
  CCAATATGGTCGACCTGTGCCCCAAAGTCCCTAAGCAGGATAACAGCAGTGACTGTGGAGTCTATTTGTT
  GCAGTATGTGGAGAGCTTTTTCCAGGATCCCATTGTTAACTTTGAACTTCCAATTCACTTGGAGAAATGG
  TTTCCTCGCCATGTAATAAAGACCAAACGGGAAGATATTCGAGAGCTCATTTTGAAACTTCACTTACAGC
  AACAGAAGGGCGGCAGCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217815 representing NM_001003971
  Red=Cloning site Green=Tags(s)
  
  MDRARPGRRRASSEIVTEGKRKKSSPADLQKWTRSLRNKVICLDHKKPKAARGCPPKGLPKRHLRVMLTN
  VLWTDLGREFRKTLPRKDANLCAPSKVQSDSLPSTSVDSIETCQRLDPLHQSLNLSERTPRVILTDIRQT
  ELGRKYLKIPPVTEASLSDTANLKSEQLSSSSDGSLESCQSVNHHKSFLSESGPKPSRTGDVPAKEAACG
  GQKQGDDGGVTPEMAAPHPKDFNTGNKGCDYLEEGTSNKNTSYSYSEMDHTPVSRKRKKRGRSNFHDSHN
  SKTSLDKPTEHTKEEENDSSVSRKLEESGEDSHQDPAPPEGLAPESLESEATNLRSFAAGQEPDASAASG
  RASSPNKSLESSASSEVSENSSVAVKGEALTLKEASPPGGSSEESQLLISAEPIVVSSDEEGPVEHKNSV
  ILKLQPPHEIMSENQGTSDPQLSELTLGACESVQVTSELFPYNPDVENISCIKSNSEMDLKLDFIFTCVY
  IGKIKGTPKGCVTFTKKYIKIPFQVSTNEISLTVDTARLKRFGLWESKDEDHSKRSHAILFLWLSSDYLQ
  DIQTQLENPMLSQQSKANEFIFLELNSSISQREELKLKDIMMEISTKNGNLHLSCPLPWVQALPLFQDLS
  PQEISFLHYYYASASALPTAAGADMKKKSVSQPSNSDTIKPTYTFLHKQSSGCYSLSITSSPEEEWQEVR
  NTGPVQKLIVYPPPPTKGGLGVTNEDLECLEEGEFLNDVIIDFYLKYLLLEKASDELVERSHIFSSFFYK
  CLTRKENNLTEDNPDLSVAQRRHRRVRTWTRHINIFNKDYIFVPVNESSHWYLAVICFPWLEEAVYEDCP
  QTVSQQFQGQQSQHDHKMTDNDPHTTSTVSTSAEDSQSTEVNMSVPKKMCKRPCILILDSLKAASIQNTV
  QNLREYLEVEWEVKRKTHREFSKTNMVDLCPKVPKQDNSSDCGVYLLQYVESFFQDPIVNFELPIHLEKW
  FPRHVIKTKREDIRELILKLHLQQQKGGSC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001003971
• ORF Size: 3030 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003971.1, NP_001003971.1
• RefSeq Size: 4802 bp
• RefSeq ORF: 3033 bp
• Locus ID: 66315
• Cytogenetics: 16 C1.1
• MW: 113.8 kDa
• Gene Summary: This gene encodes a SUMO deconjugating enzyme of the Sentrin/SUMO-specific protease (SENP) family. The encoded protein is a protease that exhibits deSUMOylating activity towards proteins involved in chromatin remodeling and promotes chromatin relaxation for DNA repair or transcription. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2015]