Atg9b (NM_001002897) Mouse Tagged ORF Clone

CAT#: MR217776

Atg9b (Myc-DDK-tagged) - Mouse ATG9 autophagy related 9 homolog B (S. cerevisiae) (Atg9b)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atg9b
• Synonyms: Apg9l2; Apg912; Apgdc2; eONE; Gm574; Nos3as; sONE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217776 representing NM_001002897
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAGACGAACGGGGTGGGGGGGGAGCAGGAGGCAACGGGGGCGGTGGGGAGACTTGGGGCCCAGCT
  CAGTGCCTCTGCTCCCTATGGCACTGCCACTGCCTGCTTCTCCATGCCGGGGAACTGGGGGAAGGAGGAT
  CTCTGTCTTCTCTCTGTCCCCTGCCCCTCGCACAAGAAGCTGCTCCTCTTCAGTGTTCCCTCCGGCCTCA
  GGGTCTCCCTGCCTGGTGATTCAGGAGGCAGGGGCTTCTCAGACTCCACACAATGTCTTACCCACCCCGA
  CCACACCCTCAACGCAGGCCCATCCCACAATGATACACACCTCTGCTTCCCCCTCGTGGGGGTCCCACTC
  CACCCCACCCCTGGCCTCGGCCACTCCCCCTCCCTCCTGCCCGCGCCCCCAGGACCATCCTGGGCTGCGG
  ATGGGCCCTCTGATCCCTGAGCAGGACTATGAACGGCTAGAGGACTGTGACCCTGAGGGGTCTCAAGACT
  CCCCTATCCATGGGGAGGACCACCAACCCTTGCTTCATGTACCCGAAGGACTCCGCGGTTCCTGGCATCA
  CATCCAGAACCTGGACAGTTTTTTCACCAAGATCTACAGCTATCATCAGCGGAATGGCTTTGCCTGTATC
  CTGCTGGAGGATGTCTTCCAGCTGGGGCAGTTCATTTTCATTGTCACCTTTACAACCTTCCTCCTTCGCT
  GCGTGGATTACAATGTTCTCTTCAACAACCAACCAAAGAACCATACAAGACGTGGACCATTACACAGCAA
  AGTGACCTTGTCAGATGCCATTCTACCCTCGGCCCAGTGTGCAGAGAAGATCCACGACAGTCCCCTGTTG
  GTCTTCCTCCTAGTCCTGGCTGCTGGCTTCTGGCTGTTCCAGCTGCTTCGCTCGGTCTGCAACCTCTTCA
  GCTACTGGGACATCCAGGTGTTTTACAGGGAGGCCCTGCACATTCCCCCAGAGGAGCTCAGCTCGGTACC
  CTGGGCCGAGGTGCAGTCTCGCCTCCTGGAACTGCAGAGGAGCGGTGGGCTGTGTGTGCAGCCGCGGCCG
  CTGACGGAACTGGACGTCCACCACCGCATCCTGCGCTACACCAACTACCAGGTAGCGTTAGCCAACAAGG
  GTCTGCTGCCTGCCCGCTGCCCGCTGCCCTGGGGGAGCAGCGCCGCCTTCCTCAGCCGCGGCCTGGCGCT
  CAACGTGGACTTGCTGCTCTTCCGCGGTCCCTTCTCACTCTTCCGCGGGGGCTGGGAGCTTCCCGAAGCC
  TACAAGCGCAGCGACCTCCGGGGCGTCCTGGCTAACCGCTGGCGGCGCACGGTGCTGCTGCTGGCCGCCG
  TGAACTTGGCCCTGAGCCCGCTGGTGCTGGCCTGGCAGGTATTGCACGCCTTCTACAGCCACGTGGAGCT
  GCTGCGGCGCGAGCCCGGAGCCTTCGGGGCGCGCCGCTGGTCCCGCCTGGCCCGCCTGCAGCTTCGCCAC
  TTTAACGAGCTGCCGCACGAGCTGCGTGCGCGCCTGGGCCGCGCCTACCGGCCCGCCGCCGCCTTCCTGC
  GTGCAGCGGAGCCCCCAGCTCCCCTGCGCGCGCTGCTGGCCCGCCAGCTGGTCTTCTTCTCGGGAGCTCT
  CTTTGCCGCGCTGCTAGTGCTCACCATCTACGATGAGGACGTGTTGGCCGTGGAGCATGTGCTCACTACC
  ATGACGGCGCTCGGGGTCACAGCCACTGTGGCCAGGTCCTTCATTCCAGAAGAGCAATGCCAGGGGCGTT
  CCTCCCAGCTTCTGCTGCAGGCAGCCCTGGCGCACATGCATTACCTCCCAGAGGAGCCTGGTGCAACGGG
  TGCTCGAGCCAGCTCTTACTGGCAGATGGCGCAGCTGCTTCAGTACCGAGCAGTCTCCCTCCTGGAAGAG
  CTCCTGTCTCCACTCCTGACCCCATTGTTTCTGCTCTTCTGGTTCCGCCCCCGTGCTCTGGAGATTATTG
  ACTTTTTTCATCACTTCACTGTGGATGTGGCTGGTGTAGGGGACATCTGTTCTTTTGCATTAATGGATGT
  GAAGCGCCACGGGCATCCTCAGTGGCTTTCAGAGGGACAGACAGAGGCCTCACTCTCTCAGCGTGCAGAG
  GATGGAAAGACCGAACTCTCCTTAATGCGGTTCTCCCTGGCACACCCACAATGGCAGCCACCAGGGCACA
  GCTCTAAGTTCCTTGGACACCTTCGGGGCCGGGTACAACAAGATGCAGCTGCCTGGGGTGCTCCCTCGAC
  TCGGAGTCCCCCCACCCCAGGAGTGCTCAGTGACTGTACATCCCCTCTGCCAGAAGCCTTCCTTGCCAAC
  CTCTTGGTAAATCCCCGCCCACCCCAGAGGGACCTGAGTCCCACAGCCCCCTGCCCTGCTGCAGCCACAG
  CCAGCCTCCTTGCCTCTATTTCACGAATGGTCCAGGACCCCAGCTGTGTGTCCCCAGGAGGCACAGGGGG
  CCAGAAGCTGACCCAGCTCCCAGAGCTTGTCTCTGCTGAGATGAGCCTCCACGCTATTTACCTACATCAG
  CTTCATCAACAGCAGCAGCAAGAACTATGGGGAGAGGCTTCAGCTTCCTCCCCATCCAGGCCCTGGTCCA
  GCCCTTCACAGCCAGGCTCACCTGATGAGGAGAAGCCATCTTGGTCAAGTGATGGCTCCAGTCCTGCTTC
  TAGCCCAAGACAACAGTGGGGCACCCAGAGGGCCCAGAATCTGTTCCCAAAGGGGTTTCAGGAGAACACA
  GACACCCAGAAGGAGCCCCTAACAGGCCCCTTGCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217776 representing NM_001002897
  Red=Cloning site Green=Tags(s)
  
  MVRRTGWGGSRRQRGRWGDLGPSSVPLLPMALPLPASPCRGTGGRRISVFSLSPAPRTRSCSSSVFPPAS
  GSPCLVIQEAGASQTPHNVLPTPTTPSTQAHPTMIHTSASPSWGSHSTPPLASATPPPSCPRPQDHPGLR
  MGPLIPEQDYERLEDCDPEGSQDSPIHGEDHQPLLHVPEGLRGSWHHIQNLDSFFTKIYSYHQRNGFACI
  LLEDVFQLGQFIFIVTFTTFLLRCVDYNVLFNNQPKNHTRRGPLHSKVTLSDAILPSAQCAEKIHDSPLL
  VFLLVLAAGFWLFQLLRSVCNLFSYWDIQVFYREALHIPPEELSSVPWAEVQSRLLELQRSGGLCVQPRP
  LTELDVHHRILRYTNYQVALANKGLLPARCPLPWGSSAAFLSRGLALNVDLLLFRGPFSLFRGGWELPEA
  YKRSDLRGVLANRWRRTVLLLAAVNLALSPLVLAWQVLHAFYSHVELLRREPGAFGARRWSRLARLQLRH
  FNELPHELRARLGRAYRPAAAFLRAAEPPAPLRALLARQLVFFSGALFAALLVLTIYDEDVLAVEHVLTT
  MTALGVTATVARSFIPEEQCQGRSSQLLLQAALAHMHYLPEEPGATGARASSYWQMAQLLQYRAVSLLEE
  LLSPLLTPLFLLFWFRPRALEIIDFFHHFTVDVAGVGDICSFALMDVKRHGHPQWLSEGQTEASLSQRAE
  DGKTELSLMRFSLAHPQWQPPGHSSKFLGHLRGRVQQDAAAWGAPSTRSPPTPGVLSDCTSPLPEAFLAN
  LLVNPRPPQRDLSPTAPCPAAATASLLASISRMVQDPSCVSPGGTGGQKLTQLPELVSAEMSLHAIYLHQ
  LHQQQQQELWGEASASSPSRPWSSPSQPGSPDEEKPSWSSDGSSPASSPRQQWGTQRAQNLFPKGFQENT
  DTQKEPLTGPLH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001002897
• ORF Size: 2766 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002897.3, NP_001002897.2
• RefSeq Size: 3902 bp
• RefSeq ORF: 2769 bp
• Locus ID: 213948
• UniProt ID: Q6EBV9
• Cytogenetics: 5 11.49 cM
• MW: 102.4 kDa
• Gene Summary: Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle.[UniProtKB/Swiss-Prot Function]