Dsc3 (NM_007882) Mouse Tagged ORF Clone

CAT#: MR217675

Dsc3 (Myc-DDK-tagged) - Mouse desmocollin 3 (Dsc3)

Product Images

Circular map for MR217675

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dsc3
• Synonyms: 5430426I24Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217675 representing NM_007882
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGTTCCTGAGTTCCGGAGCCCACAGTGCAGGGCACTATGCACGAAGCTGCTGCTGACCCTCTGGG
  TCTTCAGTTTTGTTGGTGAAGCCTGTAAAAAGGTGACATTTCATGTACCTTCAACACTAGAGGCAGACAA
  GATCATCGGAAGAGTTAGTTTGAAAGAGTGTCTCAGCTCGGCAGATGGCATCATGCCAAGTGATCCTGAT
  TTCAGAGTTCTAGATGATGGGTCAGTTTATCCGACCAGAGCTGTTGTATTGTCTGATGAGAAAAGATCTT
  TTACCATTCAGCTTTCTGATTCAAAGATGCAGACACAGAAAGAGATCCCTGTAATCCTGGAGCACAAGAA
  GAAGGTACTGAAGAAAAGACACACTAAGGAAACTGTCCTCAGAAGATCTAAGAGAAGATGGGCACCCATT
  CCATGTTCAATGCAAGAAAATTCCTTGGGTCCCTTCCCATTGTTTCTTCAACAGGTTCAGTCTGATGCAG
  CTCAGAACTACACAGTCTTCTATTCAATAAGTGGGCGTGGAGCTGATCAAGAACCACTTAACTGGTTTTT
  CATCGAGAGAGACACTGGGAATCTATATTGTACTCGGCCTGTGGATCGTGAGGAATATGATGTTTTTGAT
  TTGATTGCCTATGCATCCACTGCAGATGGCTATTCAGCAGATTTACCTCTCCCACTGCCCATCAAAATAG
  AGGATGAAAATGACAATTACCCTCTTTTTACAGAAGCAATTTATGCTTTTGAAGTTCCAGAAGGCAGTAG
  ACTTGGTACTGTAGTGGGAACAGTTTGTGCCACGGATAAAGATGAGCCTGACACTATGCACACACGCCTC
  AAGTACAGCATCTTGGAGCAGACCCCACCATCACCTGGGCTTTTTTCTGTGCACCCTGACACAGGTGTCA
  TCACCACCGTCTCTCACTACATGGACAGAGAGGTCGTGGACAAGTACAAACTGATAATGAAAGTTCAGGA
  CATGAATGGCCAATTTTTTGGACTAATCAGTACATCAACTTGTATCATAACTGTGCAAGATTCCAATGAC
  AATGCACCCACATTCAGACAAAATACTTATGAAACTGCTGTGGAGGAAAATACATATAATGTTGAAATCT
  TGCGCATACCTGTAGATGATAAGGACATGATTAACACTGCCAACTGGAAGGCAAATTTCACCATCTTAAA
  AGGAAATGAAAATGGATGGTTCAAAATCACAACAGACCCAGTGACAAATGAAGGTGTTCTGTGTGTTGTG
  AAGCCACTGGATTATGAAGAAAATCGTCAAGTGACCCTAGAGATTGGTGTGAACAACGAAGCTCCATTCA
  TTAAAGATGTTGCAAACCGAATTCCCACCATGAACCGGGCTATGGTTACAGTACATGTGAAGGATCAGAA
  TGAGGGGCCAGAATGCAAGCCCCCAGAGCAATATGTGCGGATCAAAGAGAACTCAGCAGTTGGGTCTAAG
  ATCAATGGCTACAAGGCCTACGACCCTGAAACCAAGAATAGCAATGGCTTAAGGTACAAAAAATTGCAGG
  ATCCTAAAGACTGGGTCAGCATTGAAGAAGTTTCGGGGTTGCTTACCATATCAAAAACCCTAGACAGAGA
  AATAATGGCTCCAAGAAATGACATGTACAATATTACAGTCATGGCAATTGACCAAGAGGGTAAATCATGC
  ACAGGAACACTTGCAGTGAACATCGAGGATGTGAATGACAACGCACCAGAGATAATTCAAGATTATATTG
  TTATCTGTAAGCCAAAGATGGGGTACACGGACATTTCAGCTGTGGATCCTGATGAACCTATCCATGGCCC
  TCCCTTTCAGTTCAACTTGGCAAACACTTCTCCAGAAGTCAACAGAATCTGGACCCTCAACCAAGTTAAT
  GATACAGCTGCTCGCCTGTCCTATCAGAAAACTGCGGATGTTCAAATATACAACGTTCCTGTTACTGTAA
  AAGACAGAGCAGGCCAATCTGCAACCAAAATCTTGAGAGTTAACCTGTGTGATTGTACTCATCCCAGCCA
  ATGTCCGCTCAGATCAAGGAGTGCAGGAATTACCCTGGGAAAGTGGGCCATCCTTGCCATCTTGCTGGGC
  ATTGCTCTGCTATTTTCGGTACTGCTAACTTTGGTATGTGGAGTTGTTACTGCCCGAAAAGGAAAACACT
  TCCCTGAAGATTTAGCGCAGCAAAACTTAATTATATCAAATACCGAAGCACCTGGGGATGACAGAGTGTG
  CTCTGCCAATGGATTCACCACCCACACTGCCAACAACTCCAGCCAAGGCTTCTGTGGTACAATGGGGTCA
  GGAATGAGGAATGGTGGGCAGGAGACCATCGAGATGATGAAAGGACACCAGACCTTGGACTCCTGCCGTG
  TGGCCGGGCATCACCATACCCTGGACTCCGGCAGGGGAGGACACATGGATACTGACAACTGCAGATACAC
  ATACTCAGAGTGGCACAGTTTCACTCAACCCCGACTTGGTGAAAAATTGCATGTGTGTAATCAGAATGAA
  GACCACATCCCATCCCAAGACTATGTCCTCACCTATAACTATGAAGGCAGAGGGTCTCCGGCCGGCTCTG
  TAGGTTGCTGCAGTGAAAAGCAGGAAGAAGAGGGACTTGACTTTTTAAACAACTTGGAACCCAAATTTCT
  TACATTGGCAGAAACATGCACAAAGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217675 representing NM_007882
  Red=Cloning site Green=Tags(s)
  
  MVVPEFRSPQCRALCTKLLLTLWVFSFVGEACKKVTFHVPSTLEADKIIGRVSLKECLSSADGIMPSDPD
  FRVLDDGSVYPTRAVVLSDEKRSFTIQLSDSKMQTQKEIPVILEHKKKVLKKRHTKETVLRRSKRRWAPI
  PCSMQENSLGPFPLFLQQVQSDAAQNYTVFYSISGRGADQEPLNWFFIERDTGNLYCTRPVDREEYDVFD
  LIAYASTADGYSADLPLPLPIKIEDENDNYPLFTEAIYAFEVPEGSRLGTVVGTVCATDKDEPDTMHTRL
  KYSILEQTPPSPGLFSVHPDTGVITTVSHYMDREVVDKYKLIMKVQDMNGQFFGLISTSTCIITVQDSND
  NAPTFRQNTYETAVEENTYNVEILRIPVDDKDMINTANWKANFTILKGNENGWFKITTDPVTNEGVLCVV
  KPLDYEENRQVTLEIGVNNEAPFIKDVANRIPTMNRAMVTVHVKDQNEGPECKPPEQYVRIKENSAVGSK
  INGYKAYDPETKNSNGLRYKKLQDPKDWVSIEEVSGLLTISKTLDREIMAPRNDMYNITVMAIDQEGKSC
  TGTLAVNIEDVNDNAPEIIQDYIVICKPKMGYTDISAVDPDEPIHGPPFQFNLANTSPEVNRIWTLNQVN
  DTAARLSYQKTADVQIYNVPVTVKDRAGQSATKILRVNLCDCTHPSQCPLRSRSAGITLGKWAILAILLG
  IALLFSVLLTLVCGVVTARKGKHFPEDLAQQNLIISNTEAPGDDRVCSANGFTTHTANNSSQGFCGTMGS
  GMRNGGQETIEMMKGHQTLDSCRVAGHHHTLDSGRGGHMDTDNCRYTYSEWHSFTQPRLGEKLHVCNQNE
  DHIPSQDYVLTYNYEGRGSPAGSVGCCSEKQEEEGLDFLNNLEPKFLTLAETCTKR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007882
• ORF Size: 2688 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007882.3, NP_031908.3
• RefSeq Size: 5305 bp
• RefSeq ORF: 2691 bp
• Locus ID: 13507
• UniProt ID: P55850
• Cytogenetics: 18 11.11 cM
• MW: 100.7 kDa
• Gene Summary: This gene encodes a member of the cadherin family of proteins that mediates adhesion in desmosomes. Together with desmogleins, the encoded protein forms the transmembrane core of desmosomes, a multiprotein complex involved in cell adhesion, organization of the cytoskeleton, cell sorting and cell signaling. Mice lacking the encoded protein exhibit a pre-implantation lethal phenotype. This gene is located in a cluster of desmosomal cadherin genes on chromosome 18. This gene encodes distinct isoforms, some or all of which may undergo similar processing to generate the mature protein. [provided by RefSeq, Jul 2016]