Brap (NM_028227) Mouse Tagged ORF Clone

CAT#: MR217615

Brap (Myc-DDK-tagged) - Mouse BRCA1 associated protein (Brap)

Product Images

Circular map for MR217615

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Brap
• Synonyms: 3010002G07Rik; BRAP2; IMP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217615 representing NM_028227
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGTGTCACTGGTGGTCATCCGACTGGAGCTCGCGGGTCACTCGCCGGTCCCCACCGACTTCGGCT
  TCAGCGCCGCCGCTGGAGAGATGTCTGATGAGGAGATCAAAAAGAAGACATTGGCTTCCGCTGTCGCCTG
  TCTAGAAGGGAAGTCAGCCGGGGAGAAAGCAGCCATCATCCACCAGCACCTTGGCCGTCGGGAAATGACG
  GATGTGATTATTGAGACCATGAAGGCCAGAGCAGATGAAGTGAGAGACACAGTGGAAGAAAAGAAGCCTT
  CAGCAGCCCCTGTCTCTGCACAGAGAAGTAGAGAACAGAGTGAGTCTGTGAACACAGCCCCAGAGTCTCC
  ATCCAAACAGCTCCCAGACCAGATTTCCTTCTTCAGTGGCAACCCTTCTGTTGAAATAGTCCACGGCATT
  ATGCACCTGTACAAGACAAATAAGATGACCTCCCTAAAAGAAGACGTTCGGCGCAGTGCCATGCTGTGTG
  TCCTCACCGTCCCTGCCACCATGACCAGTCATGACCTTATGAAGTTTGTTGCCCCATTCAATGATGTAAT
  TGAACAAATGAAAATCATCAGGGACTCTACTCCGAATCAGTACATGGTACTGATCAAGTTCAGTGCGCAG
  GCTGATGCAGACAGTTTCTACATGGCGTGCAATGGCCGCCAGTTCAACTCAATCGAAGATGACGTCTGCC
  AGCTGGTCTATGTGGAAAGGGCTGAAGTGCTGAAATCTGAAGATGGCGCCAGCCTCCCCGTGATGGACCT
  GACGGAGCTGCCCAAGTGCACTGTGTGTCTGGAGCGGATGGACGAGTCTGTGAATGGCATCCTCACCACC
  CTCTGCAACCACAGCTTCCATAGTCAGTGTCTGCAGCGGTGGGATGACACCACGTGTCCTGTGTGCCGAT
  ACTGTCAAACGCCAGAGCCAGTGGAAGAAAACAAATGTTTTGAGTGTGGTGTCCAGGAAAACCTCTGGAT
  TTGTTTAATATGCGGCCACATAGGCTGTGGGCGGTACGTGAGTCGGCATGCTTACAAGCACTTTGAGGAG
  ACCCAGCACACATACGCCATGCAGCTCACCAACCATCGAGTCTGGGACTATGCTGGAGATAATTATGTCC
  ATCGACTGGTTGCAAGCAAGACGGATGGAAAGATCGTTCAGTACGAGTGTGAGGGCGACACCTGCCAGGA
  AGAGAAGATAGATGCCTTACAGTTAGAGTACTCGTACCTGTTGACAAGCCAGCTGGAATCGCAGCGGATA
  TACTGGGAGAACAAAATCGTGCGCATAGAGAAGGACACGGCAGAGGAGATTAACAACATGAAGACCAAGT
  TTAAAGAGACCATCGAGAAGTGTGACAGCCTGGAGCTCAGGCTCAGTGACCTCCTGAAGGAGAAGCAGTC
  TGTGGAAAGGAAGTGTACCCAGCTGAACACCAGAGTGGCCAAGCTCAGCACGGAGCTGCAGGAGGAGCAG
  GAGCTGAACAAGTGTCTGCGCGCCAACCAGCTGGTGCTGCAGAACCAGCTCAAGGAGGAGGAGAAGCTGC
  TGAAGGAGACCTGTGCCCAGAAAGACCTGCAGATCACCGAGATCCAGGAGCAGCTGCGCGATGTCATGTT
  CTACCTGGAGACACAGCAGCAGATCAGCCACCTGCCTGCGGAGACGAGGCAGGAGATCCAGGAAGGCCAG
  ATCAACATCGCCATGGCCTCAGCGCCCAACCCACCCTCTTCCGGGGCCGGTGGGAAGCTGCAGTCCAGAA
  AGGGCCGCAGCAAGAGGGGCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217615 representing NM_028227
  Red=Cloning site Green=Tags(s)
  
  MSVSLVVIRLELAGHSPVPTDFGFSAAAGEMSDEEIKKKTLASAVACLEGKSAGEKAAIIHQHLGRREMT
  DVIIETMKARADEVRDTVEEKKPSAAPVSAQRSREQSESVNTAPESPSKQLPDQISFFSGNPSVEIVHGI
  MHLYKTNKMTSLKEDVRRSAMLCVLTVPATMTSHDLMKFVAPFNDVIEQMKIIRDSTPNQYMVLIKFSAQ
  ADADSFYMACNGRQFNSIEDDVCQLVYVERAEVLKSEDGASLPVMDLTELPKCTVCLERMDESVNGILTT
  LCNHSFHSQCLQRWDDTTCPVCRYCQTPEPVEENKCFECGVQENLWICLICGHIGCGRYVSRHAYKHFEE
  TQHTYAMQLTNHRVWDYAGDNYVHRLVASKTDGKIVQYECEGDTCQEEKIDALQLEYSYLLTSQLESQRI
  YWENKIVRIEKDTAEEINNMKTKFKETIEKCDSLELRLSDLLKEKQSVERKCTQLNTRVAKLSTELQEEQ
  ELNKCLRANQLVLQNQLKEEEKLLKETCAQKDLQITEIQEQLRDVMFYLETQQQISHLPAETRQEIQEGQ
  INIAMASAPNPPSSGAGGKLQSRKGRSKRGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028227
• ORF Size: 1773 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028227.3, NP_082503.2
• RefSeq Size: 3727 bp
• RefSeq ORF: 1776 bp
• Locus ID: 72399
• UniProt ID: Q99MP8
• Cytogenetics: 5 F
• MW: 67.4 kDa
• Gene Summary: Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport (By similarity).[UniProtKB/Swiss-Prot Function]