Gsdmc3 (NM_183194) Mouse Tagged ORF Clone

CAT#: MR217410

Gsdmc3 (Myc-DDK-tagged) - Mouse gasdermin C3 (Gsdmc3)

Product Images

Circular map for MR217410

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gsdmc3
• Synonyms: 9930109F21Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217410 representing NM_183194
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTACAGTTTTGACCGGGCTAGCAAGGATGTGGTTAAAAAGCTTCAAGGAAGAGATCTGAGGCCTG
  TGGAATGCTTGTCAGATGCAACCAAATTCCGCCTATTTCACATACTCCAGGAGACTCCTCGATCAGGTTG
  GGAAACTGAAGATATTCCAGTTGGATTTACCCTTCTGGATCTCTTGGAGCCCAACTTTCCTGTTCCAGAG
  CCTGAAGTGTCTGCTCCCAAGCCCTTCATACATGTCCAATCAACGGACCTGGAGGCTAACTTGAATGTCG
  CAGACATTGCAAGAGGAGGTGTAGGGTATGTTGGGTACGGTGGATACAACATCGAGGTTCAGAGTACAAG
  CATCCCAAATCCAAAACTGGAAATTCTTCAAAACAGGAAACTGTTGGATAAGTTGCCCACATTTATGAAG
  TTTTGCCGGATGGAAAGGAAGAACCTGTATGTGGTGACAGAGGCTTATGAAGTGTCCAAGGATACTATGC
  TGACAGGCTTAAGCAGTGTCAATCTTTTAGTTAAAGGGTTCTTCAAACAGCTTTTCAAGGTTCGAGGCAA
  GGCGGGAAGGTCGGAAAAGTATTCAATACCTATCCCAAAGGGTTCGGTGTTGGCCTATAAGAAGCAGCAA
  CTTGTCATTGAGAATAACACTTGTGTCATTCTCCCTTCTGCTACTAAGAAGAAGATGACCTTTCCAGGCA
  CACCTAAGTACGCTTCTGCTTCCGAACCCACTGAGATTTATAGAACAGAATTACAAGGTTTGTGGATAAA
  TGACATCGAACCAATTGGAAGAATCCAGGAGCCCGCCCATCTAGATTTCAAGTGCCTACAATATGAAGTT
  TCTGAACAAACACGACTACTGCCTGAACTCTCAAAGGATGTTCAAGAAGTTGTGTTGTCCAGTTTCCTGT
  CCATGCTCTATGAAGGTGACAGGAACGTTCTGCATGACCTGATGAAAATGCTGGAGTTGAGTCAGTTGGG
  GCATATGGATGGCCCAGGTGGCAAAATCCTGGATGAGCTTCGAAAGGATTCTAGCAACCCATGTGTTGAC
  CTAAAGGACCTCATTCTTTATCTCCTTCAAGCGCTGATGGTGCTCAGTGATAGCCAACTCAATCTGCTGG
  CCCGGTCTGTGGAGATGCGGCTCCTCCCGCACCAAGTAGAGCTGGTAACGAGCATCCTACAGCCAAACTT
  CAAGTATCCCTGGAACATTCCCTTCACAGTCCAGCCTCAGCTTCTTGCCCCACTCCAGGGTGAGGGCTTG
  GCCATTACTTATGAATTGCTGGAGGAGTGTGGTCTGAAGATGGAGCTGAATAATCCCAGGTCAACTTGGG
  ATTTGGAAGCCAAGATGCCCTTGTCTGCTCTCTATGGAAGCCTGTCATTTTTACAGCAACTTCAGAAGGC
  TAACTCTTCCTTCAAGCCCAGCCTCCGACCTGGATACATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217410 representing NM_183194
  Red=Cloning site Green=Tags(s)
  
  MGYSFDRASKDVVKKLQGRDLRPVECLSDATKFRLFHILQETPRSGWETEDIPVGFTLLDLLEPNFPVPE
  PEVSAPKPFIHVQSTDLEANLNVADIARGGVGYVGYGGYNIEVQSTSIPNPKLEILQNRKLLDKLPTFMK
  FCRMERKNLYVVTEAYEVSKDTMLTGLSSVNLLVKGFFKQLFKVRGKAGRSEKYSIPIPKGSVLAYKKQQ
  LVIENNTCVILPSATKKKMTFPGTPKYASASEPTEIYRTELQGLWINDIEPIGRIQEPAHLDFKCLQYEV
  SEQTRLLPELSKDVQEVVLSSFLSMLYEGDRNVLHDLMKMLELSQLGHMDGPGGKILDELRKDSSNPCVD
  LKDLILYLLQALMVLSDSQLNLLARSVEMRLLPHQVELVTSILQPNFKYPWNIPFTVQPQLLAPLQGEGL
  AITYELLEECGLKMELNNPRSTWDLEAKMPLSALYGSLSFLQQLQKANSSFKPSLRPGYI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_183194
• ORF Size: 1440 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183194.3, NP_899017.2
• RefSeq Size: 2142 bp
• RefSeq ORF: 1443 bp
• Locus ID: 270328
• UniProt ID: Q8CB12
• Cytogenetics: 15 D1
• MW: 54.2 kDa
• Gene Summary: The N-terminal moiety promotes pyroptosis and exhibits bactericidal activity. The physiological relevance of these observations is unknown.[UniProtKB/Swiss-Prot Function]