Abcg3 (NM_030239) Mouse Tagged ORF Clone

CAT#: MR217194

Abcg3 (Myc-DDK-tagged) - Mouse ATP-binding cassette, sub-family G (WHITE), member 3 (Abcg3)

Product Images

Circular map for MR217194

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abcg3
• Synonyms: Abc; Abcp2; Mxr; Mxr2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217194 representing NM_030239
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCTAATAATGACCCCACTGTAATCTCAATGATAGAAAGACATCTTTGTGACCTTCCTGAAACAA
  ACACCAGTGACCTGAAGACATTGACTGAAGAGGCTGTGTTGAGTTTTCACAACATCAGTTACCAAGAAAC
  AGTACAGAGTGGCTTTCCACTTCGTAAAAAGGCATATGTGATAGAAAGACTATCAAACATCAGTGGGATC
  ATGAAACCTGGCCTCAATGCCATTATGGGACCTCAGGATGGGAGCAGATCTTTGTTGTTAGATGTCTTAG
  CTGCAAGGAGAGATCCGCGTGGATTATCAGGAGATATTTTGATAAATGGCAAACCTCGACCTGCTAATTT
  CAAGTGTACTTCTGGTTATGTACCACAAAATGATGTGGTGTTGGGCACAGTGACCGTGAGAGACAATTTA
  GAGTTCTCAGCAGCTCTTCGTCTCCCAGTGACTATAACAAGAGATGAAAAGAGAAGGAGGATTAATGAGG
  TGCTTGAACTTCTGCATCTGAATAAAGAACAAAATATCAAGCCTAGATCCAAAGAACTCAGGAAAAGGAC
  CAGTATAGCAATGGAGCTGGTCACTGAGCATCCCATTTTGTTCTTGGATGATCCCACAACTGGCTTAGAT
  TTGAGAACTACGACTGATGTCATTTTAGTCCTGAGAAGGATGTCGAAGAAGGGACGGACCATCATCTTCT
  CCATCAATCAGCCTCAGTACTCCATCTTCAAATTTTTTGATAGCCTCACCTTAGTGGCTTCAGGAAAAGT
  TATGTTTCATGGACCTGCACAGGATGCTTTGGAGTACTTCAGATCTGCAGGTTACAATTATGAATCGCAC
  AACAACCCTGCAGACTTCTTCCTGGATGTCATTAATGGAGGTTTCTCTAATATATTAGACACAGAGGAAG
  ACGGCCATGAAGATGACAAATATGAAGAGCTTTTTGAGAGACAGTATCAAGTCACAGGAAAACTGGCCAA
  TATGTATGCCCAGTCCCCATTATACAGTGAAACAAGAGCCATCCTGGATCAACTCTTGGGAGAACAGAAG
  CTGGAGAGGAGCTCAGCCGTGGAGACAACATGTGTCACCCCTTTCTGCCATCAGCTCAAGTGGATTATCT
  GTCAATCATTCAAAAATTTCAAGGGTTTTCCATGGGTCACTGTGATTCAGGCCATTATCACAGTCATATT
  GGCTACAGCCGTAGGCACTGCTTTCCGTGTCCTAAAAAATGATTGTATTGAAGTCCAGATGAGAGCTGGC
  CTACTTTACCTGCTCACCATCTTCCAGTGTATCACAAGTGTGTCTGCAGGGGAGCTCTTTGTGATTGACA
  GGGTTCGCTTTCTACATGAGCACACCAGTGGATACTACAGAGTGTCATCCTATTTCTTTGGGAAATTGCT
  GGCTGAGCTAATACCCAGGAGGCTTTTGCCAAGTACTGTATTTTCTCTAATAACATATGTCATAGCAGGA
  GTAAAAATGAGTATGAAGTGTTTCTTCACTATGATATGTACCATCATGGTGCTGGCCTATTCTGCCAGTT
  CCCTGCCATTGTCCATAGGAGCTGGGGAGAATGCAGTGGCTGTTCCAACACTGCTTGTGACCATCTACTT
  TGTGTTCATGCTGTTTTTTTCAGGTCTATCACTATATTCTGGAAGCTTCCTGCCTAAGCTCTCATGGATT
  CAGTACTTTAGCATTCCTCATTATGGATTTAGGGCTCTGCTGCATAATGAATTCCTGGGACAAAACTTTT
  GTCCAGAACACAACACAGAAGAAGTCAGTAGATGCCATAACTATGTTATATGTACTGGTGAAGAATTCTT
  GATGATCCAGGGCATTGATCTCTCATCGTGGGGCTTCTGGGAGAATCACCTGGCTTTAGTTTGTACAATG
  ATTATCCTCTTAACAATTACCTATGTGCAGTTATTACAGGTTAAGAATATTAGAAATTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217194 representing NM_030239
  Red=Cloning site Green=Tags(s)
  
  MASNNDPTVISMIERHLCDLPETNTSDLKTLTEEAVLSFHNISYQETVQSGFPLRKKAYVIERLSNISGI
  MKPGLNAIMGPQDGSRSLLLDVLAARRDPRGLSGDILINGKPRPANFKCTSGYVPQNDVVLGTVTVRDNL
  EFSAALRLPVTITRDEKRRRINEVLELLHLNKEQNIKPRSKELRKRTSIAMELVTEHPILFLDDPTTGLD
  LRTTTDVILVLRRMSKKGRTIIFSINQPQYSIFKFFDSLTLVASGKVMFHGPAQDALEYFRSAGYNYESH
  NNPADFFLDVINGGFSNILDTEEDGHEDDKYEELFERQYQVTGKLANMYAQSPLYSETRAILDQLLGEQK
  LERSSAVETTCVTPFCHQLKWIICQSFKNFKGFPWVTVIQAIITVILATAVGTAFRVLKNDCIEVQMRAG
  LLYLLTIFQCITSVSAGELFVIDRVRFLHEHTSGYYRVSSYFFGKLLAELIPRRLLPSTVFSLITYVIAG
  VKMSMKCFFTMICTIMVLAYSASSLPLSIGAGENAVAVPTLLVTIYFVFMLFFSGLSLYSGSFLPKLSWI
  QYFSIPHYGFRALLHNEFLGQNFCPEHNTEEVSRCHNYVICTGEEFLMIQGIDLSSWGFWENHLALVCTM
  IILLTITYVQLLQVKNIRNF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030239
• ORF Size: 1950 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030239.2, NP_084515.2
• RefSeq Size: 3034 bp
• RefSeq ORF: 1953 bp
• Locus ID: 27405
• UniProt ID: Q99P81
• Cytogenetics: 5 50.68 cM
• MW: 73.6 kDa
• Gene Summary: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It lacks several highly conserved residues found in other ATP-binding proteins; this suggests that this protein may not bind ATP and may require dimerization with another subunit to form a functional ATP-transporter. The function of this gene has not yet been determined; however, high levels of expression in the thymus and spleen suggest a potential role in the transport of specific peptides or hydrophobic compounds from lymphocytes. [provided by RefSeq, Jul 2008]