H1f3 (NM_145713) Mouse Tagged ORF Clone

CAT#: MR217111

Hist1h1d (Myc-DDK-tagged) - Mouse histone cluster 1, H1d (Hist1h1d)

Product Images

Circular map for MR217111

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: H1f3
• Synonyms: H1-3; H1.3; H1D; H1s-; H1s-4; Hist1h; Hist1h1d
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217111 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGAGACCGCTCCCGCGGCGCCTGCTGCCCCTGCACCTGTGGAGAAGACACCTGTGAAGAAGAAGG
  CGAAGAAGACCGGCGCCGCTGCTGGGAAGCGCAAGGCGTCCGGACCCCCGGTGTCCGAGCTCATCACCAA
  GGCTGTGGCCGCCTCCAAGGAGCGCAGCGGCGTGTCCCTGGCTGCGCTCAAGAAGGCGCTGGCGGCCGCG
  GGGTACGATGTGGAGAAGAACAACAGCCGCATCAAGCTCGGGCTGAAGAGCCTGGTGAGCAAGGGTACCC
  TGGTGCAGACCAAGGGCACCGGCGCCTCCGGCTCCTTCAAACTCAACAAGAAGGCGGCTTCCGGTGAGGC
  TAAGCCCAAGGCTAAGAAGGCAGGCGCGGCCAAGGCCAAGAAGCCTGCGGGAGCAGCCAAGAAGCCTAAG
  AAGGCGACTGGTGCTGCCACACCCAAAAAGACGGCCAAGAAGACTCCGAAGAAGGCGAAGAAGCCTGCGG
  CGGCTGCCGGCGCCAAGAAAGTTTCCAAGAGTCCCAAGAAGGTGAAGGCTGCTAAGCCCAAGAAGGCAGC
  AAAGAGTCCAGCCAAGGCCAAGGCTCCCAAGGCTAAGGCTTCCAAGCCTAAAGCTTCTAAGCCGAAGGCC
  ACCAAGGCAAAGAAGGCTGCCCCTCGCAAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217111 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSETAPAAPAAPAPVEKTPVKKKAKKTGAAAGKRKASGPPVSELITKAVAASKERSGVSLAALKKALAAA
  GYDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAASGEAKPKAKKAGAAKAKKPAGAAKKPK
  KATGAATPKKTAKKTPKKAKKPAAAAGAKKVSKSPKKVKAAKPKKAAKSPAKAKAPKAKASKPKASKPKA
  TKAKKAAPRKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145713
• ORF Size: 666 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145713.4, NP_663759.3
• RefSeq Size: 776 bp
• RefSeq ORF: 666 bp
• Locus ID: 14957
• UniProt ID: P43277
• Cytogenetics: 13 A3.1
• MW: 22.1 kDa
• Gene Summary: Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. [provided by RefSeq, Sep 2015]