Ldb3 (NM_001039074) Mouse Tagged ORF Clone

CAT#: MR216832

Ldb3 (Myc-DDK-tagged) - Mouse LIM domain binding 3 (Ldb3), transcript variant 5

Product Images

Circular map for MR216832

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ldb3
• Synonyms: AW742271; PDLIM6; ZASP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216832 representing NM_001039074
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTACAGTGTGACTCTGACTGGGCCTGGGCCCTGGGGCTTCCGTCTGCAGGGAGGCAAGGACTTCA
  ACATGCCCCTCACTATCTCCCGGATCACTCCAGGCAGCAAGGCAGCCCAGTCCCAGCTCAGCCAAGGAGA
  CCTGGTGGTGGCCATTGATGGCGTCAACACAGACACCATGACCCACCTGGAGGCCCAGAACAAGATCAAG
  TCGGCCAGCTACAACCTGAGCCTCACTCTACAGAAGTCCAAGCGGCCTATTCCCATCTCCACGACAGCGC
  CTCCCATCCAGTCCCCGCTGCCAGTGATCCCCCACCAGAAGGTGGTAGCCAATTCTCCAGCCAATGCTGA
  CTACCAGGAACGCTTCAACCCCAGCGTCCTGAAGGACTCAGCCCTGTCCACCCACAAGCCCATTGAAGTG
  AAGGGCTTGGGAGGCAAGGCCACTATCATCCACGCTCAGTACAACACCCCGATCAGCATGTACTCACAGG
  ATGCTATCATGGACGCCATCGCGGGGCAGGCCCAGGCCCAGGGCAGTGACTTCAGTGGGGCGTCTCCGCT
  GGCGAGCCTCCCTGTGAAAGACTTGGCGGTGGACAGCGCCTCTCCTGTGTACCAGGCTGTGATCAAAACC
  CAAAGCAAGCCGGAAGATGAGGCTGATGAGTGGGCCCGCCGGTCCTCCAACCTGCAGTCTCGCTCCTTCC
  GCATCCTGGCCCAGATGACCGGGACAGAATACATGCAAGACCCTGATGAAGAGGCTCTGCGAAGGTCAAG
  CACCCCTATTGAGCATGCTCCAGTGTGCACCAGCCAGGCCACTTCCCCGCTGCTGCCTGCTTCTGCCCAG
  TCGCCCGCTGCTGCCTCTCCCATTGCGGCCTCGCCAACCCTGGCCACAGCTGCTGCCACCCATGCTGCTG
  CCGCCTCTGCTGCAGGCCCTGCCGCAAGTCCCGTGGAGAATCCGAGGCCACAGGCCTCTGCTTACAGTCC
  TGCAGCCGCCGCTTCTCCAGCACCCTCTGCCCATACCAGCTACAGCGAGGGTCCAGCTGCCCCTGCACCC
  AAGCCTCGGGTCGTAACCACTGCCAGCATCCGGCCTTCTGTCTACCAGCCAGTGCCTGCATCTTCCTACA
  GCCCATCTCCAGGAGCCAATTACAGTCCAACTCCCTATACCCCTTCGCCAGCTCCTGCTTATACCCCCTC
  TCCTGCTCCCACCTATACCCCCTCACCTGCACCTACATACTCCCCTTCCCCTGCACCAGCTTATACCCCC
  TCACCTGCCCCAAACTATACCCCTACACCATCAGCTGCCTACAGTGGGGGCCCTTCAGAATCTGCCAGCC
  GTCCACCCTGGGTGACCGATGACAGCTTCTCTCAGAAGTTTGCTCCTGGAAAGAGCACTACCACTGTCAG
  CAAGCAGACCCTGCCCCGGGGGGCTCCAGCCTACAACCCAACAGGTCCACAAGTGACACCTCTTGCCAGG
  GGCACCTTCCAGAGAGCTGAGCGCTTCCCAGCTAGCAGCCGGACTCCCCTCTGTGGGCACTGTAACAATG
  TCATCAGGGGCCCTTTTCTGGTGGCCATGGGCCGCTCTTGGCACCCAGAAGAGTTCAATTGTGCCTACTG
  CAAGACCTCCCTAGCAGATGTGTGCTTTGTGGAGGAGCAGAACAACGTTTACTGCGAGCGGTGTTACGAG
  CAGTTCTTTGCCCCGATCTGTGCCAAATGTAACACCAAAATCATGGGGGAAGTGATGCACGCTCTGCGAC
  AGACATGGCACACCACCTGCTTTGTCTGTGCAGCCTGCAAGAAGCCCTTTGGGAACAGCCTCTTCCACAT
  GGAGGATGGAGAGCCATACTGTGAGAAAGACTACATCAACCTGTTCAGCACCAAGTGCCATGGATGCGAT
  TTCCCTGTGGAAGCTGGTGACAAGTTTATCGAGGCCCTGGGGCACACCTGGCATGACACCTGCTTCATTT
  GTGCGGTCTGCCACGTGAATCTGGAGGGTCAGCCATTCTACTCTAAGAAAGACAAACCACTGTGCAAGAA
  GCACGCACATGCCATCAACGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216832 representing NM_001039074
  Red=Cloning site Green=Tags(s)
  
  MSYSVTLTGPGPWGFRLQGGKDFNMPLTISRITPGSKAAQSQLSQGDLVVAIDGVNTDTMTHLEAQNKIK
  SASYNLSLTLQKSKRPIPISTTAPPIQSPLPVIPHQKVVANSPANADYQERFNPSVLKDSALSTHKPIEV
  KGLGGKATIIHAQYNTPISMYSQDAIMDAIAGQAQAQGSDFSGASPLASLPVKDLAVDSASPVYQAVIKT
  QSKPEDEADEWARRSSNLQSRSFRILAQMTGTEYMQDPDEEALRRSSTPIEHAPVCTSQATSPLLPASAQ
  SPAAASPIAASPTLATAAATHAAAASAAGPAASPVENPRPQASAYSPAAAASPAPSAHTSYSEGPAAPAP
  KPRVVTTASIRPSVYQPVPASSYSPSPGANYSPTPYTPSPAPAYTPSPAPTYTPSPAPTYSPSPAPAYTP
  SPAPNYTPTPSAAYSGGPSESASRPPWVTDDSFSQKFAPGKSTTTVSKQTLPRGAPAYNPTGPQVTPLAR
  GTFQRAERFPASSRTPLCGHCNNVIRGPFLVAMGRSWHPEEFNCAYCKTSLADVCFVEEQNNVYCERCYE
  QFFAPICAKCNTKIMGEVMHALRQTWHTTCFVCAACKKPFGNSLFHMEDGEPYCEKDYINLFSTKCHGCD
  FPVEAGDKFIEALGHTWHDTCFICAVCHVNLEGQPFYSKKDKPLCKKHAHAINV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039074
• ORF Size: 2052 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039074.2, NP_001034163.1
• RefSeq Size: 4954 bp
• RefSeq ORF: 2055 bp
• Locus ID: 24131
• UniProt ID: Q9JKS4
• Cytogenetics: 14 B
• MW: 73.2 kDa
• Gene Summary: May function as an adapter in striated muscle to couple protein kinase C-mediated signaling via its LIM domains to the cytoskeleton.[UniProtKB/Swiss-Prot Function]