Alg10b (NM_001033441) Mouse Tagged ORF Clone

CAT#: MR216801

Alg10b (Myc-DDK-tagged) - Mouse asparagine-linked glycosylation 10 homolog B (yeast, alpha-1,2-glucosyltransferase) (Alg10b)

Product Images

Circular map for MR216801

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Alg10b
• Synonyms: AA469671; AU045404; AW123895; C85569; Deaf1; Gm917; nse5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216801 representing NM_001033441
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCAGCTGGAGGGCTATTACTTCTCGGCCGCCTTGAGCTGCACCTTCCTGGTGTCCTGCCTGCTCT
  TCTCCGCCTTCAGCCGCGCTCTGCGGGAGCCGTACATGGACGAGATCTTCCACCTGCCGCAGGCGCAGCG
  GTACTGCGAGGGCCGCTTCTCCCTGTCGCAGTGGGATCCTATGATCACTACATTACCTGGCCTGTATCTG
  GTGTCGGTCGGTGTGGTCAAACCTGCCAGCTGGCTCCTTGGATGGTCTGAGCATGTGATCTGCTCCATTG
  GGGTGCTCAGATTTGTCAATCTTCTCTTCAGCGTTGGCAACTTCTACTTGCTGTATTTGCTCTTCAGGAA
  GGTGCAACCCAGAAACAAGGCTTCTTCAAGTATCCAGAGAATCTTGTCAACATTAACCCTAGCAGTGTTT
  CCAACCCTCTATTTTTTTAACTTCCTTTACTATACAGAAGCTGGGTCTGTATTCTTCACTCTTTTTGCTT
  ATTTGATGTGTCTTTATGGCAACCATAGGACCTCGGCCTTGCTTGGATTTTGTGGTTTCATGTTTCGTCA
  GACCAACATCATCTGGGCGGCCTTCTGTGCAGGACACCTCATTGCACAGAAGTGCAGTGAAGCCTGGAAG
  ATTGAGCTGCAGAAGAAGAAGGAGGAGAGGCTTGCGCCCACGAAGGGACCGCTCTCGGAGCTCAGAAGAG
  TGCTGCAGTTTCTGCTGGTGTACGCCATGTCCCTAAAGAACCTGAGAATGCTCTTCCTGTTGACCTGGCC
  CTACGTGCTTCTGCTGTTGGCGTTCTTTGCCTTTGTGGTAGTTAATGGTGGCATTGTTGTTGGTGACCGG
  AGTAGTCACGAGGCCTGTCTCCATTTTCCTCAGTTGTTCTATTTCTTCTCCTTTACTGCCTTTTTCTCCT
  TCCCTCACCTACTTTCTCTGACCAAAGTCAAGACTTTCCTTAGCTTAGTTTGGAAGCGTAGAGTCCAGTT
  CTCTGTGGTTACGTTAGTCTCGATACTTTTGGTTTGGAAATTCACTTATGTCCATAAGTATTTACTGGCA
  GACAATAGGCATTACACATTTTATGTGTGGAAAAGAGTATTTCAGAGACATGAAGTTGTCAAATATTTAT
  TAGTTCCAGCCTACATTTTTGCTGGTTGGGCTATAGCTGACTCTTTAAAGGCTAAGTCAATTTTCTGGAA
  TTTAATGTTTTTTGTATGCTTGGTTGCTTCTACAGTTCCTCAGAAACTACTAGAATTCCGTTACTTCATT
  CTACCATACATTATTTATAGGCTTAACATACCTCTGCCACCCATATCTAGACTCGTTTGTGAACTGGGTT
  GCTATACAGTTGTTAATTTTGTAACTTTTTATATCTTTCTGAACAAGACTTTTCAGTGGCCAAATAGTCA
  GGACATCCAAAGGTTTATGTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216801 representing NM_001033441
  Red=Cloning site Green=Tags(s)
  
  MAQLEGYYFSAALSCTFLVSCLLFSAFSRALREPYMDEIFHLPQAQRYCEGRFSLSQWDPMITTLPGLYL
  VSVGVVKPASWLLGWSEHVICSIGVLRFVNLLFSVGNFYLLYLLFRKVQPRNKASSSIQRILSTLTLAVF
  PTLYFFNFLYYTEAGSVFFTLFAYLMCLYGNHRTSALLGFCGFMFRQTNIIWAAFCAGHLIAQKCSEAWK
  IELQKKKEERLAPTKGPLSELRRVLQFLLVYAMSLKNLRMLFLLTWPYVLLLLAFFAFVVVNGGIVVGDR
  SSHEACLHFPQLFYFFSFTAFFSFPHLLSLTKVKTFLSLVWKRRVQFSVVTLVSILLVWKFTYVHKYLLA
  DNRHYTFYVWKRVFQRHEVVKYLLVPAYIFAGWAIADSLKAKSIFWNLMFFVCLVASTVPQKLLEFRYFI
  LPYIIYRLNIPLPPISRLVCELGCYTVVNFVTFYIFLNKTFQWPNSQDIQRFMW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033441
• ORF Size: 1422 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033441.3, NP_001028613.1
• RefSeq Size: 3727 bp
• RefSeq ORF: 1425 bp
• Locus ID: 380959
• UniProt ID: Q3UGP8
• Cytogenetics: 15 E3
• MW: 55.9 kDa
• Gene Summary: Putative alpha-1,2-glucosyltransferase, which adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc(2)Man(9)GlcNAc(2)-PP-Dol (By similarity). When coupled to KCNH2 may reduce KCNH2 sensitivity to classic proarrhythmic drug blockade, possibly by mediating glycosylation of KCNH2 (By similarity). Has a role in maintenance of cochlear outer hair cell function (PubMed:24303013).[UniProtKB/Swiss-Prot Function]