Peak1 (NM_172924) Mouse Tagged ORF Clone

CAT#: MR216525

C230081A13Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA C230081A13 gene (C230081A13Rik)

Product Images

Circular map for MR216525

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Peak1
• Synonyms: 1110049L02Rik; 9530046P14; AA690169; BE132841; C230081A13Rik; mKIAA2002; SGK269
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216525 representing NM_172924
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCTTGTAACACCTTTACTGAACACGTTTGGAAACCTGGCGAATGCAAGAATTGCTTTAAACCTA
  AAAGCTTGCACCAGCTTCCTCCAGACTCTGAGAAGACGCCCATCACCCACGGCAGTGGGAAAACTAATGC
  CAATCACAGTAACAACCACCGAGTCAGGAGCACGGGCAACTTCCGGCCCCCTGTGGCTAAAAAGCCCACC
  ATCGCTGTGAAGCCCACTATGATGGTGGCAGATGGGCAAAGCGTGTGTGGCGAGCTAAGCATCCAGGAAC
  ACTGTGAGAACAAGCCTGTCATCCTGGGGTGGAACCAAAACAAGACGTCCCTGAGCCAGAAACCACTTAA
  CAACAATTCTGAAGGTGATGCTGAAGGCTTTGGCAGTGATCCTCAGCAGTGTGCCAATAATGACAGTGCA
  CAGAAGATATCCAATAACAACAACGGGCTAACTGAAGTGCTAAAGGAGATAGCGGGCCTGGAAGCCACCC
  CTCCGGTCAGAGGAAATGAAACAAACGCCAGAGAAACATTCTTGGGAAGAATAAATGATTGCTACAAACG
  ATCGTTGGAAAGAAAGATCCCACCCAGTTGCATGACCGGGAGCATGAAGGACTCTCAGGGCAAACACGTC
  ATCCTGAGCGGGAGCGCCGAGGTCATCAGTAACGAAGGGGGCAGGTTCTGCTACCCTGAGTTCTCCAGTG
  GTGAGGAGAGTGAGGAAGATGTGCTTTTCAGTAACATGGAGGAGGAGCATGAGAGCTGGGATGAGAGCGA
  TGAAGAGCTGTTGGCCATGGAGATTCGCATGAGAGGGCAGCCTCGCTTTGCTAACTTCAGGGCAAACACC
  CTGTCTCCTGTTCGATTCTTTGTGAGCAAAAAGTGGAATACTATCCCCTTACGGAATAAGTCTCTGCAGA
  GAATCTGTGCTGTGGACTATGACGACAGCTATGATGAAATCCTGAATGGTTATGAGGAAAATTCTGGGGT
  CTCCTATGGTCAAGGAAGCGTTCAGAGCACAATATCATCTGACTGCACATCACCAGGCTCCTCCTTCACA
  GAAGAGTCACGCTCTGAAACAGCCAGCAGTTTATCCCAGAAGGTCTGTAATGGGGGGATATCTCCTGGTA
  ACCCAGGAAATTCTAAGGACATAGCAGAAACTGAGTCTAATTTTGAAAGTCCTCCTGGTAACAATGAGGA
  GAAGGACGAATCACTGACTTCCAAAAGCTCGGTGAAAGTTCCAGAGACGCACAAAGCTGTCCTTGCTCTC
  CGTTTACAGGAGAAGGATGGCAAGATTGCAGTACATACTGAGAAGCCAGAGAGCAAAGCTTCTACAGATA
  TCGCTGGCCAGGCAGTCACCATAAGCCTCGTCCCAGTAGAAGAACAGACAAAGCCCTACCGTGTGGTAAA
  TCTGGAACAGCCGCTGTGTAAGCCATATACCGTTGTGGATGTGTCGGCAGCCATGGCCAGCGAGCACCTG
  GGGCGACCCAAGATAAAAGGTTCATCTTCTACTCCAAACTCTCCAGTTACATCACCTGCATTGACACCAG
  GACAAATAAATGCCCATCTCAAAAAATCCAGTGCAATCCGATACCAGGAAGTGTGGACGTCCAGCACCAG
  TCCACGACAAAAGATCCCTAAAATAGAATTAAGTACTGGGGGACCTGGGCCAAATGTCCCTCCAAGGAAA
  AACTGCCATAAGTCGGCGCCCACTTCACCCACGGCTACAAACATCTCCTCTAAAACCATCCCTGTTAAGT
  CACCTAATTTGTCTGAAATAAAATTTAATAGTTACAACAATGCCGGGATGCCACCTTTTCCCATTATCAT
  CCATGATGAGCCATCCTATGCCCGGAGTTCCAAAAATGCCATTAAAGTCCCCATTGTTATTAATCCAAAT
  GCGTATGACAATCTCGCCATCTACAAAAGTTTTCTGGGAACAAGTGGAGAACTCTCTGTGAAGGAAAAAA
  CCACAAGCGTAATAAGCCATACTTACGAAGAAATAGAAACTGAAAGCAAAGTGTCTGATAGCACCCCCAG
  CAAGCTCACAGACTGTCCCCAAGCTAAAGGGTTTTCCAACAGCACAGAGCGGAAAAGAGGCTCAGTAGCG
  CAGAAGGTGCAGGAGTTTAACAATTGTCTCAACAGAGGTCAGTCTTCTCCACAAAGAAGCTACAGTTCCA
  CCCACAGCTCCCCAGCAAAAATCCAGAGACCCACTCAAGAGCCTGCGGGCAAAACGGAAGGGGCACAAGG
  GTCTCAGGTGCCAGGCAGCAGCAGCAACAGCACTAGGGAGAAAGCAAGCGCCGTCCTATGTCAGATTGTG
  GCATCTATCCAACCCCCCCAGACTCCTCCAGAAGCACCTCAATCTAGCCCTAAGGCTTGCAGTGTGGAAG
  AGCTTTATGCCGTTCCCCCAGATGCAGATACTACAAAAAGTATTCCTAAAAACCCACCAGTCAGACCCAA
  ATCTCTTTTTACATCTCAGTCTAGTGGGGAAGGTGAGGCCCATCAGACCACAGAAAGCCCTACTGCCAAA
  ATTCAGAAAGATCCATCCACGAAGCCAGTGACTTCTCCTCCCTCCAAATTAGTGACGAGCGCCCAGAGTG
  AGCCACCGCCTCCCTTTCCCCCACCACGGTCTACTTCCTCCCCTTACCATGCAAGTAACCTGTTGCAAAG
  GCATTTTACCAATTGGACGAAGCCAACCAGTCCTACCCGATCAACAGAAGCTGAATCAATTTTGCACTCT
  GAAGGCAGCAGGCGAGCCGCTGATGCAAAACCTAAACGCTGGATATCATTTAAAAGCTTCTTCCGCCGCC
  GCAAAACAGATGAGGAAGAAGAGAAGGAGAAAGAGCGAGAGAAAGGGAAGCTGGTGGGCCTGGACGGGAC
  AGTCATCCACATGCTGCCGCCCCCTCCAGTCCAGCGCCATCACTGGTTCACAGAGGCCAAAGGAGAGGCC
  AGCGAGAAGCCTGCTATAGTCTTCATGTACAGGTGTGACCCTGACCAAGGCCACCTCAGTGTGGACCAAA
  GCAAGGCTGGGGCAGAGAAAGGAAGAGCAGAGGAGGTGTTACTGCGGAACTCGGAAGAGAAGAAAAGTAG
  CTATCTTCCATCACAGATTCCTGACAAAGCTTGCAGTCGTGTGACCCATGAAGTGGCAGGAGAACTTTCT
  CCTCGGGATCCAAGAACTCCTGCTGGGAAGCAAGATGGCACTTCAGTCACACCAACACTGCCTCCACCTG
  ACCTGGAAAGGGAAGAAGAAAAAGATGACACTTTGGATCCTACTGACGTGAGTCCCTGCAGTGCAACCTA
  CAGCAACCTAGGACAATCTCGAGCAGCTATGATACCTCCCAAGCATCCACGGCATCCCAAGGGAGCGGTG
  GATGATGCCATTGCCTTTGGAGAGAAGACAGACCAAGAAGGACTGAATGCTTCCCAACCCACACCACCCC
  CACTGCCAAAGAAAATGATACGAGCCAATACCGAGCCCATCTCCAAAGACCTTCAGAAAGCCATGGAAAG
  CAGTCTTTGCGTCATGGCTAATCCCACCTACGATATTGATCCCAACTGGGATGCCAGCAGTGCTGGGTCT
  TCTATTAGCTATGAACTCAAGGGACTAGACGTTGAGTCTTATGAATCCTTGGAGAGACCTTTGCATAAGG
  AGAGACCCGTTCCTTCAGCAGCAAACAGTATCTCCAGCCTAGCCACTCTCAGTGTTAAGGACAGATTTTC
  TAACAGTATGGAGTCACTCTCCAGTCGACGTGGTCTCTCCTATAGACAGACTCGAAGCATCCAGAAGCCA
  CAGAGACAAGCACTTTATCGAGGACTTGACAATCGGGAGGAAGTGGTGGGTAAACTCCGAAGCCTTCATA
  CAGATGCCTTGAAGAGACTGGCAGTTAAATGTGAAGACCTCTTCATGGCTGGGCAGAAAGACCAACTCCG
  CTTTGGAGTAGACAGCTGGTCAGACTTCAGGCTAACCAGCGACAAACCGTGCTGCGAAGCTGGAGATGCG
  GTTTACTACACTGCCTCGTATGCAAAAGATCCACTCAGTAACTATGCAGTCAAGATCTGCAAGAGCAAAG
  CTAAGGAGTCTCAGCAGTATTACCATAGCCTGGCTGTCCGGCAGAGTCTGCCTGTGCATTTTAACATCCA
  GCAGGATTGTGGCCATTTCCTTGCTGAAGTCCCTAGTCGTCTGCTTCCCTGGGAGGATCCTGATGCTCCT
  GAAAAGGCAGAGGATGGCACTGAAGACAGCGAAGAAGAGGGGAAGGCAGAAACTTTGGGGGGAAATCCTG
  AGCCCTGCTCTGAAACAGAGCCATCCCAGAAAGAGAACCAACGAGTCACAAACAGGAAACAAAGAAGCCA
  CGTTGTGGTCATCACCAGGGAGGTTCCCCATCTCACTGTGGCCGACTTCGTGCGAGATTCTTTGGCCCAT
  CATGGGAACAGCCCTGACTTGTATGAGAGGCAAGTGTGTCTGCTGCTCTTACAGCTGTGCTCTGGGCTTG
  AGCACCTCAAGCCCTACCACGTCACGCACTGCGATCTTCGCCTGGAGAACTTGCTGCTTGTCCAGCACCA
  ACCTGGGGGAGCTGCCCAGGGCCCTTCTCCTGCAGACCCTTGCCCCACCTTAGCTTGTCCTACAAGGCTC
  ATTGTGAGTAACTTCTCTCAGGCCAAGCAGAAGAGCCACCTGGTGGACCCCCAGATCCTCCGAGACCAGT
  CCCGCCTTGCCCCAGAGATCATCACGGCCACCCAGTATAAGAAGTGTGATGAATTCCAGACGGGCATTCT
  CATCTATGAGATGCTGCACCTGCCCAACCCTTTTGACGAGAACCCGGAGCTGAAGGAGAAGGAGTACACG
  CGCACAGATCTGCCTCGGATCCCTCTCCGCTCCCCGTACTCCTGGGGTCTACAGCAGCTTGCTAGCTGCC
  TCCTGAATCCCAACCCTTCTGAGCGCATCCTCATTTCTGACGCCAAAGGCATCCTCCAATGTCTGCTCTG
  GGGCCCCCGAGAAGATCTCTTTCAAATCTTCACCACCTCTGCAACCCTCGCACAGAAGAACGCTCTGCTC
  CAAAACTGGTTAGACATCAAGCGAACCCTGCTGATGATCAAGTTCGCGGAGAAGTCCCTGGACAGAGAGG
  GGGGCATCAGCCTGGAGGACTGGCTTTGTGCTCAGTATCTGGCCTTTGCCACTACAGACTCCCTGAGCTA
  TATTGTAAAAATCCTGCAATACCGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216525 representing NM_172924
  Red=Cloning site Green=Tags(s)
  
  MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDSEKTPITHGSGKTNANHSNNHRVRSTGNFRPPVAKKPT
  IAVKPTMMVADGQSVCGELSIQEHCENKPVILGWNQNKTSLSQKPLNNNSEGDAEGFGSDPQQCANNDSA
  QKISNNNNGLTEVLKEIAGLEATPPVRGNETNARETFLGRINDCYKRSLERKIPPSCMTGSMKDSQGKHV
  ILSGSAEVISNEGGRFCYPEFSSGEESEEDVLFSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANT
  LSPVRFFVSKKWNTIPLRNKSLQRICAVDYDDSYDEILNGYEENSGVSYGQGSVQSTISSDCTSPGSSFT
  EESRSETASSLSQKVCNGGISPGNPGNSKDIAETESNFESPPGNNEEKDESLTSKSSVKVPETHKAVLAL
  RLQEKDGKIAVHTEKPESKASTDIAGQAVTISLVPVEEQTKPYRVVNLEQPLCKPYTVVDVSAAMASEHL
  GRPKIKGSSSTPNSPVTSPALTPGQINAHLKKSSAIRYQEVWTSSTSPRQKIPKIELSTGGPGPNVPPRK
  NCHKSAPTSPTATNISSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPSYARSSKNAIKVPIVINPN
  AYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKVSDSTPSKLTDCPQAKGFSNSTERKRGSVA
  QKVQEFNNCLNRGQSSPQRSYSSTHSSPAKIQRPTQEPAGKTEGAQGSQVPGSSSNSTREKASAVLCQIV
  ASIQPPQTPPEAPQSSPKACSVEELYAVPPDADTTKSIPKNPPVRPKSLFTSQSSGEGEAHQTTESPTAK
  IQKDPSTKPVTSPPSKLVTSAQSEPPPPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESILHS
  EGSRRAADAKPKRWISFKSFFRRRKTDEEEEKEKEREKGKLVGLDGTVIHMLPPPPVQRHHWFTEAKGEA
  SEKPAIVFMYRCDPDQGHLSVDQSKAGAEKGRAEEVLLRNSEEKKSSYLPSQIPDKACSRVTHEVAGELS
  PRDPRTPAGKQDGTSVTPTLPPPDLEREEEKDDTLDPTDVSPCSATYSNLGQSRAAMIPPKHPRHPKGAV
  DDAIAFGEKTDQEGLNASQPTPPPLPKKMIRANTEPISKDLQKAMESSLCVMANPTYDIDPNWDASSAGS
  SISYELKGLDVESYESLERPLHKERPVPSAANSISSLATLSVKDRFSNSMESLSSRRGLSYRQTRSIQKP
  QRQALYRGLDNREEVVGKLRSLHTDALKRLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDA
  VYYTASYAKDPLSNYAVKICKSKAKESQQYYHSLAVRQSLPVHFNIQQDCGHFLAEVPSRLLPWEDPDAP
  EKAEDGTEDSEEEGKAETLGGNPEPCSETEPSQKENQRVTNRKQRSHVVVITREVPHLTVADFVRDSLAH
  HGNSPDLYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVQHQPGGAAQGPSPADPCPTLACPTRL
  IVSNFSQAKQKSHLVDPQILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYT
  RTDLPRIPLRSPYSWGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQIFTTSATLAQKNALL
  QNWLDIKRTLLMIKFAEKSLDREGGISLEDWLCAQYLAFATTDSLSYIVKILQYR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172924
• ORF Size: 5205 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172924.3, NP_766512.2
• RefSeq Size: 10994 bp
• RefSeq ORF: 5208 bp
• Locus ID: 244895
• UniProt ID: Q69Z38
• Cytogenetics: 9 B
• MW: 191.5 kDa
• Gene Summary: Probable catalytically inactive kinase. Scaffolding protein that regulates the cytoskeleton to control cell spreading and migration by modulating focal adhesion dynamics. Acts as a scaffold for mediating EGFR signaling.[UniProtKB/Swiss-Prot Function]