Mypop (NM_145579) Mouse Tagged ORF Clone

CAT#: MR216399

Mypop (Myc-DDK-tagged) - Mouse Myb-related transcription factor, partner of profilin (Mypop)

Product Images

Circular map for MR216399

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mypop
• Synonyms: Myodysa; P42pop
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216399 representing NM_145579
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCGGCGACCGCGGCGGCGGCGCCGGGCGAAGCGGAGGAGACCACCCGACTCCGCAAGCCGCGCT
  TCTCCTTCGAGGAGAACCAGATCCTGATCCGAGAGGTGCGCGCCCACTACCCTCAGCTCTACGGCGCGCA
  GAGCCGTCGGGTGAGCGTGGCCGAGCGGCGGCGAGTGTGGGACAGCATCGCCACCAAAATCAACGGCATC
  ACCAGCTGGAAACGCACGGGCCAGGAGGTTCAGAAGCGTTGGAACGACTTCAAGCGCCGCACCAAGGAGA
  AGCTGGCCCGCGTGCCGCATTCCACGCAGGGCGCGGGGCCCGCCGCTGAGGACGCCTTCTCTGCGGAGGA
  GGAGACTATTTTCGCCATCCTGGGACCGGGTGTGGCGGGGCCGGGGGCTGGCTCTGGGGCTGAGGAGTCC
  CGGGCAGCCGCCTCTTCACAGCCGCAGGCTTCGACCGCTAGTACACAACGGTATGTGTTGTCAGAGGACC
  GCAGGCAGGATCGACGGGCAGATACACCAGCCCAGAGCAAGGGAGGCTCCAGCAGCCCAGAGTCCTGGGC
  CCGGCCCTCTTGCAATCCCCAAGAAGCCAAGGAGCGTGAGTCCACGTCCCCTGCAGCCATGCAGCCTGTC
  CAGCTGCCCCGCCTGGCCTTGTCTCCACCGCTCCCTGCCCCTCCACCACCACCCACGGCGCTGGCCCAAG
  TGGCACCCTCATCTCCTAGCCCAACTCCTCCTCGGCCCACCTCAGCCCCAGAACAGTCCCTGGACTTCCT
  GCGGGCTCAGCAGGAGACTGCCAATGCTATCAGGGAGCTGGCTGGCACCCTTCGGCAGGGACTGGCCAAA
  CTGAGCGAGGCCCTCAGTGCCCTGTTACCCCTTTTGCCGGGAACCCCAGCTGACCCGCTGCCCCCGCCCC
  CACCGCCACCTCCGCCCCCACCTCCCAAACCAGTCCTGCCACCCTCTGCCCCCAAGGTGGAGCTCGCCCC
  AGAGCCTGTGTCTGTGGTAGCTGCTGTTGTGGATGGGGCTGTAGTGGCAGCGAGGGGAGTGATCATCTCC
  CCCAGGAGCGAGGAAGGGGTGCCCAAACCACTACCCCCAGCCCCACCACTTCCCCTCCACGACTCACCCC
  CACACAAAAGGAGGAAAGGTTTCCCTACACGAAAGAGGCGGGGCCGCTGGAAGTCTCCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216399 representing NM_145579
  Red=Cloning site Green=Tags(s)
  
  MASATAAAAPGEAEETTRLRKPRFSFEENQILIREVRAHYPQLYGAQSRRVSVAERRRVWDSIATKINGI
  TSWKRTGQEVQKRWNDFKRRTKEKLARVPHSTQGAGPAAEDAFSAEEETIFAILGPGVAGPGAGSGAEES
  RAAASSQPQASTASTQRYVLSEDRRQDRRADTPAQSKGGSSSPESWARPSCNPQEAKERESTSPAAMQPV
  QLPRLALSPPLPAPPPPPTALAQVAPSSPSPTPPRPTSAPEQSLDFLRAQQETANAIRELAGTLRQGLAK
  LSEALSALLPLLPGTPADPLPPPPPPPPPPPPKPVLPPSAPKVELAPEPVSVVAAVVDGAVVAARGVIIS
  PRSEEGVPKPLPPAPPLPLHDSPPHKRRKGFPTRKRRGRWKSP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145579
• ORF Size: 1179 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145579.3, NP_663554.1
• RefSeq Size: 2188 bp
• RefSeq ORF: 1182 bp
• Locus ID: 232934
• UniProt ID: Q8R4U1
• Cytogenetics: 7 A3
• MW: 41.9 kDa
• Gene Summary: Transcriptional repressor; DNA-binding protein that specifically recognizes the core sequence 5'-YAAC[GT]G-3'. Dimerization with PFN1 reduces its DNA-binding capacity.[UniProtKB/Swiss-Prot Function]