Plekhm2 (NM_001033150) Mouse Tagged ORF Clone

CAT#: MR216370

Plekhm2 (Myc-DDK-tagged) - Mouse pleckstrin homology domain containing, family M (with RUN domain) member 2 (Plekhm2)

Product Images

Circular map for MR216370

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Plekhm2
• Synonyms: 2310034J19Rik; AI854247; mKIAA0842
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216370 representing NM_001033150
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGCGGGAGGTGAAGGACCGAATCCTGGAGAACATCTCGCTGTCGGTGAAGAAGCTGCAGAGCT
  ACTTTGCAGCATGTGAGGACGAGACCCCTGCCATCCGGAACCACGACAAGGTCCTGCAGCGCCTGTGTGA
  GCACCTGGACCACGCCCTGCTGTACGGGTTGCAAGACTTGTCCTCTGGCTACTGGGTGCTCGTGGTCCAT
  TTTACCCGCAGAGAGGCCATCAGGCAAATTGAGGTGCTACAGCATGTAGCCACCAACCTGGGGCGAAGCC
  GAGCCTGGCTCTACCTGGCCCTCAATGAGAATTCCTTGGAGAGCTACCTGCGTCTGTTCCAGGAGAACCT
  GGGCCTGCTACAGAAGTACTATGTCAGGAACGCCCTGGTCTGCAGCCACGATCACCTGACTCTCTTCCTG
  ACCTTGGTGTCTGGGTTGGAGTTTATTCGGTTCGACCTGGATCTGGACGCCCCGTACTTAGACCTGGCTC
  CGTACATGCCTGACTATTATAAACCTCAGTACCTGCTGGACTTTGAAGACCGCCTTCCTAGCTCAGTCCA
  CGGTTCAGACAGCCTCTCCCTAAACTCCTTCAATTCTGTCACCTCCACCAACCTGGAATGGGACGACAGT
  GCAATTGCCCCATCTAGCGAGGATTATGATTTTGGAGATGTGTTTCCAGCAGTGCCGTCTGTACCCAGCA
  CAGACTGGGAAGATGGAGACCTCACGGACACCATCAGTGGTCCGCGTTCCACTGCTTCAGACCTGACCAG
  TAGCAAGACATCCACCAAGAGCCCCACCCAGCGTCATAACCCCTTCAATGAGGAGCAAGCAGAGACTGCA
  TCATCCGACACCACCCCTGTGCACACTACCTCTCAGGAGAAGGAGGAGGCCCAGGCCCCAGACCAGCCAG
  ACGCTTGTACAGAGCTTGAAGTTATCAGGGTCACCAAGAAGAAGAAGATTGGCAAGAAGAAAAAGACCAA
  GCTGGACGAGGATGCAAGCCCACTGCACCCCACCTCTAGTCAGCAGAAATGTGGCCAGCAGGGGGAAGGT
  GATGGACTTGTGGGCACCCCAGGCCTGGCGAGGGACCCTTCGGACACTGTACTTGCTTCCCCTCAGGAGC
  AAGGGGAAGGACTCAGCAGTACGGCTGGGAGCAGCGAGCTCTCCGAGCTCAGCCAGATGGGCTTGCTCAT
  CCCCGAGATGAAGGACACCTCCATGGAGTGCCTAGGACAGCCTCTGAGCAAGGTCATTGACAAACTCCAC
  GGCCAGCTGGACCCTAGCACCTGGTGCTCCCATGCCGATCCTCCCGAGCAGTCCTTTCGGGCCGGCTCTC
  CGGGGGAGGCCCCGGAGAAACCACCATTTTGCGACTTTAGTGAGGGGCTTCCAGCCCCCATGGACTTCTA
  CCGGTTTACAGTCGAGAGTCCAAGTACTGTTGCACCAGGTGGCGGCCACCATGACCCTCCAGGGCCTAGC
  CAACCGCTGCATGTTCCTGGTAGCCCTGCGGCTGCTCTCCAAGAAGAAGAAGAAGGAGGAGGAAGAGGAG
  AGGGACAGACATCTCAGCCCCTAGAAGACCGGCAAGGAGAGGAGATTCAGGAGCCAGAGCCTCAGGAACC
  GGACAGCCAGTTGCCACTGGTCAGCCAGGAGCCCGTGCCCGAACCTGTGTCCCAGCCTGAACCTGGAACC
  CACGAGGCTCTTTGCAAGCTCAAGCGAGACCAGCCCAGTCCTTGTCTGAGCAGTGCTGAGGACTCCGGGG
  TAGAGGAGGGCCAGGGTAGCCCTTCAGAGATGACGCACCCCTCAGAGTTCAGAGTAGACAACAACCACTT
  GCTCCTCCTCATGATTCACGTCTTTCGAGAAAACGAAGAGCAGTTGTTCAAAATGATCCGGATGAGCACA
  GGGCACATGGAGGGTAATCTGCAACTGCTCTACGTGCTACTCACAGACTGCTATGTCTACCTGCTTCGGA
  AAGGGGCCACAGAAAAGCCATACCTGGTGGAAGAGGCTGTTTCTTACAATGAACTTGACTATGTGTCGGT
  TGGCCTCGACCAACAGACTGTCAAGCTGGTGTGCACCAACCGCAGGAAGCAGTTTCTGCTGGACACAGCC
  GACGTGGCCCTGGCTGAGCTCTTCTTGGCGTCTCTGAAGTCAGCCATGATCAAAGGTTGCCGGGAACCAC
  CCTACCCCAGCATCCTGACTGATGCCACCATGGAGAAGCTGGCGTTGGCGAAATTTGTCGCCCAGGAGTC
  CAAATGTGAGGCATCCGCCGTGACCGTGCATTTCTATGGGCTTGTGCACTGGGAGGACCCCATGGAAGAG
  GCCCTGGGCCCTGTTCCCTGCCAGTGCTCACCCGCTGAGGGCACCATCACCAAAGAAGGCATGCTGCACT
  ACAAGGCTAGCACCTCCTACCTGGGCAAGGAACACTGGAAGGCCTGCTTCGTGGTGCTCAGCAATGGGAT
  CCTCTACCAGTATCCTGACCGCACGGACGTCATCCCCCTGCTGTCCGTGAACATGGGTGGGGAACAGTGC
  GGTGGCTGCCGGAGATCCAACACCACGGATCGGCCCCACGCCTTCCAGGTCATTCTGGCTGACCGGCCCT
  GCCTGGAGCTAAGCGCCGACAGCGAGGCTGAGATGGCTGACTGGATGCAACATCTCTGCCAGGCCGTGTC
  CAAAGGAGTCATACCCCAGGGCATAGCTCCCAGCCCCTGTATCCCTTGCTGCCTGGTGATCACAGAGGAC
  CGCCTCTTCACCTGCCACGAGGATTGCCAAACCAGCTTCTTTCGCTCCCTGGGCACAGCCAGGCTGGCAG
  ACATCACTGCCATCTCCACGGAGCTGGGCAAGGAATACTGTGTCCTGGAGTTCTCCCAGGATAGCCCACA
  GCTGCTCCAGCCTTGGGTCATCTACCTGAGCTGCACTTCCGAGCTGGACCGGTTCCTGACTGCCCTGAGC
  TCTGGGTGGAAAGCCATCTACCAGGTAGACCTTCCTCACAAGGCCATCCATGAAGCTTCCATCAAGCAGA
  AATTTGAAGACGCCCTGAGCCTCATCCACAGCGCCTGGCAGCGGAGCGACAGTCTCTGCCGCGGCAGGGC
  CTCCCGGGACCCCTGGTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216370 representing NM_001033150
  Red=Cloning site Green=Tags(s)
  
  MEPREVKDRILENISLSVKKLQSYFAACEDETPAIRNHDKVLQRLCEHLDHALLYGLQDLSSGYWVLVVH
  FTRREAIRQIEVLQHVATNLGRSRAWLYLALNENSLESYLRLFQENLGLLQKYYVRNALVCSHDHLTLFL
  TLVSGLEFIRFDLDLDAPYLDLAPYMPDYYKPQYLLDFEDRLPSSVHGSDSLSLNSFNSVTSTNLEWDDS
  AIAPSSEDYDFGDVFPAVPSVPSTDWEDGDLTDTISGPRSTASDLTSSKTSTKSPTQRHNPFNEEQAETA
  SSDTTPVHTTSQEKEEAQAPDQPDACTELEVIRVTKKKKIGKKKKTKLDEDASPLHPTSSQQKCGQQGEG
  DGLVGTPGLARDPSDTVLASPQEQGEGLSSTAGSSELSELSQMGLLIPEMKDTSMECLGQPLSKVIDKLH
  GQLDPSTWCSHADPPEQSFRAGSPGEAPEKPPFCDFSEGLPAPMDFYRFTVESPSTVAPGGGHHDPPGPS
  QPLHVPGSPAAALQEEEEGGGRGEGQTSQPLEDRQGEEIQEPEPQEPDSQLPLVSQEPVPEPVSQPEPGT
  HEALCKLKRDQPSPCLSSAEDSGVEEGQGSPSEMTHPSEFRVDNNHLLLLMIHVFRENEEQLFKMIRMST
  GHMEGNLQLLYVLLTDCYVYLLRKGATEKPYLVEEAVSYNELDYVSVGLDQQTVKLVCTNRRKQFLLDTA
  DVALAELFLASLKSAMIKGCREPPYPSILTDATMEKLALAKFVAQESKCEASAVTVHFYGLVHWEDPMEE
  ALGPVPCQCSPAEGTITKEGMLHYKASTSYLGKEHWKACFVVLSNGILYQYPDRTDVIPLLSVNMGGEQC
  GGCRRSNTTDRPHAFQVILADRPCLELSADSEAEMADWMQHLCQAVSKGVIPQGIAPSPCIPCCLVITED
  RLFTCHEDCQTSFFRSLGTARLADITAISTELGKEYCVLEFSQDSPQLLQPWVIYLSCTSELDRFLTALS
  SGWKAIYQVDLPHKAIHEASIKQKFEDALSLIHSAWQRSDSLCRGRASRDPWC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033150
• ORF Size: 3099 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033150.2
• RefSeq Size: 4178 bp
• RefSeq ORF: 3102 bp
• Locus ID: 69582
• UniProt ID: Q80TQ5
• Cytogenetics: 4 E1
• MW: 114.4 kDa
• Gene Summary: May play a role in the regulation of conventional kinesin activity. Required for maintenance of the Golgi apparatus organization. May play a role in membrane tubulation. May play a role in lysosomes movement and localization at the cell periphery.[UniProtKB/Swiss-Prot Function]