Abca6 (NM_001166557) Mouse Tagged ORF Clone

CAT#: MR215883

Abca6 (Myc-DDK-tagged) - Mouse ATP-binding cassette, sub-family A (ABC1), member 6 (Abca6), transcript variant 3

Product Images

Circular map for MR215883

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abca6
• Synonyms: 6330565N06Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215883 representing NM_001166557
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGAGCTAAGTGTGCACGTGCGCCAGCAGACCCGCGCCCTTCTGCACAAGATTCTGCTCAAGAAAT
  GGCGGAGGAAGAGAGAGAGCTTATTGGAATGGAGCATACCAATTATTATAGGACTGCATATGGGTCTGTT
  TTCCTACTTGGCAAGAAATATTCAGGTCCTCGAAGTCCCTCCTCAGGATCTTGGAAGTCTAAATGAATTT
  AATGGATCTTCTCTAGTAGTTGTATATACACCGATCTCTAACATAACCCAGCAGATAATGAACAAAACAA
  CATTTGCTCCAACTATGAAAGGAACAAGAATCATCGGGGTACCAAGTATAGAAGACTTGGATGAAGTTCT
  TCTGCACAATATACCGGACGCTTTAGGAGTCATCTTTAATGACAGTTTCTCTTACCAATTAAAAGTCCTC
  AGGATGTATGGAAATCCATTTTTGAAAGAGGACTTATTAGCTCATTGCTGGGATACGCATTCTCAGGCGT
  TCTGTTCACTTTCCAAATACTGGGAAAGAGGCTTCGTAGCTTTACAAACCGCCATTAACGCTGGGATTAT
  AGAAGTCACAACAAACCACTCGGTGATGGAGGAGCTGATGTCCATCGATGGGATCAATATGAAGACATTA
  CCTTTCATTCCTAGAGATCTTTCGGATTACGAAATTTTTATCTTGTTCTGCTTGCTTTATTTCTCCTCGT
  TTATATATTTTGCATCCAGCAATGTTACTAAAGAGAGAAAACAGTGTAAGGAGGTCATGAAAGTCATGGG
  TCTACAAGACTCTGCATTCTGGCTGTCCTGGGGCTTAATCTATGTTGGCTTCATCTTTATTATTTCCATA
  TTCATTGCAATTATCATAACATCTACCCAGATTATAATGATGACTGGTTTCCTGGTCATATTTACGCTGT
  TTTTCTTATATGGATTATCCTTGGTAAGTGTATGTGTTTACTACCATCATTTACACATTATATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215883 representing NM_001166557
  Red=Cloning site Green=Tags(s)
  
  MKELSVHVRQQTRALLHKILLKKWRRKRESLLEWSIPIIIGLHMGLFSYLARNIQVLEVPPQDLGSLNEF
  NGSSLVVVYTPISNITQQIMNKTTFAPTMKGTRIIGVPSIEDLDEVLLHNIPDALGVIFNDSFSYQLKVL
  RMYGNPFLKEDLLAHCWDTHSQAFCSLSKYWERGFVALQTAINAGIIEVTTNHSVMEELMSIDGINMKTL
  PFIPRDLSDYEIFILFCLLYFSSFIYFASSNVTKERKQCKEVMKVMGLQDSAFWLSWGLIYVGFIFIISI
  FIAIIITSTQIIMMTGFLVIFTLFFLYGLSLVSVCVYYHHLHIIS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166557
• ORF Size: 975 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166557.1, NP_001160029.1
• RefSeq Size: 1441 bp
• RefSeq ORF: 978 bp
• Locus ID: 76184
• UniProt ID: Q8K441
• Cytogenetics: 11 E1
• MW: 37.8 kDa
• Gene Summary: Probable transporter which may play a role in macrophage lipid homeostasis.[UniProtKB/Swiss-Prot Function]