Cep95 (NM_177088) Mouse Tagged ORF Clone

CAT#: MR215875

Cep95 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 45 (Ccdc45), transcript variant 1

Product Images

Circular map for MR215875

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cep95
• Synonyms: 4732496G21Rik; AI118346; AI448335; Ccdc45; D330027A14Rik; F630025I20Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215875 representing NM_177088
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCTCAGAGGCTGAATGGGTAACAATTGCCAATAACCTCCTTTTTAAATGTCACATACACCTGA
  GGATACACGAACTTCAAGACTGTGATGCTAATGTTTTTATTGCCCTTTATCAATCCATTTTGGGAGAAAA
  GGTACCAGACCTCATAGTTCTTCCCAGGAATCAAGAAGACGAGGCCCACAATGTCCAAGCGGTAATTGAT
  TCACTCGCTTTGGATTACCTTCAGGTCAGCCTGTCTCACATAACAGGAGAAAACATAGTGAAAGGGGATA
  ATGAATCTATCAGGAATCTCCTAGAAATATTTGATGGATTGCTGGATTATCTGACAGAACACATCAGTGA
  ATCGTCTCCCAACAAAAGTGAAACTGAGCAGTATTCTAAAGATTCTCATGGAGAAGAAGCTGGGGAGGAC
  CTAGAACGTACCGAGGAGGCGAAGTGGAGAAACGCTTCTTTTATGAGGTGTTCCTTCTCATCGGACACTT
  TGGGCCCCACCTGGGATGAAGACGAAGCTGAGTCCACAGGTGAAATAATTAGGCTGGGAGACACTGCACA
  TACCTTTTCCCAAAGAAGTAATGGTGCTCAAAATTCTAAGGACTTACGGTCTAGAAAAGCCTCGGCTTCA
  CCAGGCGTTGAGCCACCTGAGGAAATGTTGAACCCTGGCCCATTGGGTTTTCTTTCCCAGAATGGGCCAC
  CTTGTGAGGCAGCATCAGAAACTCCTCCTATGAGCATGGTTCCAAGTGCCAGGAAGCTAGGCGAGCCGAT
  CCGAGCTGCCATTCCTTTACATCCTCCCTACCATCCTTCTGAGCCTAGAGCACCATGTCCCATAGGCAAA
  GAATACCTCTGGTCAAGTCGCTACTTGTCTACGCCGACTTCTGGAGAGCACATGGCACCTTCTGTGGAAC
  CAGATGATGCTTTCTTAACTTCTACCTTATTTAAAGATGATGATCAGGAAACATATCTACCTAAACCTGA
  AGCCACCAGGACAAGGAAGCCTTCTAAAGGAGAAAGAGATGAAAACAGAGCTGCGATCCCCTCAGAGCAT
  CTGCCTTTCTCTCAGAAGGCCAGGAAGCCGTTAACAGAACAAGAACTGCACGCCATGTCAGAGAAACTCT
  CTCAGCGGCTCTCTGAACTAGACTGGATGTTAAAAACTGCCCTGGGTGATCGAGGAACGGGCGAAACTGA
  TGGTAATGATGGTGATGGCGGAGGTGAGGAGGTGCGCAGTGGGAACGAGGAGATGCTCTCGCAGCACAGT
  GACAGCGTCATGGAGTACGGACCAAAGAAGCCGAGGCCAGGATTTTCTACGTGCAGAAAGGCACCTTACA
  GGTCCCATTCACTGTCGCCATCTTCAGTGAACAAACACAGACAATTAGAGAAAGAGAAAAAAAGGCAGCA
  CAAATCTAAAGGGACAGACTCATGCCATTTCCAAGCAAAGGCACTGACTGAAGCATTTGAACGGGAACTA
  AGAAAACATAAAGTTCAAGAGAACGTTGGACTTCGAGGGATAAGGGAGGAGGAGGAAGAGGAAGAGGAAA
  CAGGGAAGTCATACAGAGAAGTCGTTCCTAAAGGAACTTCCAAACGGAGTCAGGTCCAGAAGACTTACTC
  CAGAAAAACTGCAGCTCCAAGTCCAAAAGGAGATGGTCGCCTGAAGTCAAGCAAGGCGTCTCCGATGAAA
  GTAAGTGAACACAGTCTGCTATCCCTGATGCTGGAACAGTTTCCATTTCTCTATGTTTCTGACCCAACAC
  TGACCAAAATGTGGAAGCAGCAGATGGCCCAGGTTGAACAACTCAAAAGGGAAGCGCAGAGAGAGAACCG
  ATCCAAGAAGAAACTCCAGGATGAAATAGAAGAAGCCCTGAGAAGGCATGACCTCCTCACTGCTCTTGTC
  AAGAAAGAATATGACCATAACAAGAGGCTGCAAGACTTCAGGGACCGAATTCAGAGACAAAGGTTGACCC
  AATCAAAGATAAAAGAAAATCGTCATCAAAGTGTTCGTGCCCGGAAATATTATGATGATTACAGAGTTCA
  GTTACGTGCAAAAATGATGAAGATGAGAACCCGGGAAGAAATGATCTTTAAGAAACTGTTTGAAGAAGGT
  TTACAAATTCAGAAACAAAGACTACGAGACCTAAGAAACTACGCCAAAGAGAAACGCAACGAAGAAAAGA
  GACAGCACCAGAATGAGCTGGACTCGATGGAGAACCACTATAAGGACCAGTTTTCATTGCTAGCAGAAGC
  CATCTCACAGGAACGTCAAGAACTCAAAGTGAGACAGAAATTCCAGGCTCAGACATTACATAAGGTGAAG
  AGGGAGCTGAGAGCTAAAATGGAGAAGGAGATTCAGCAACTGCAGCACATGATAACGCAGAACGACGATG
  ATGCTTTCTTCCGGGAGCTAGAAGCTGAGCGCTTCAAAGCTCGGCTGCAGTTGGCTTCCTTCCAGTACAG
  TAAAAATCCTTTCCCAAGAGGCCAAACTTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215875 representing NM_177088
  Red=Cloning site Green=Tags(s)
  
  MASSEAEWVTIANNLLFKCHIHLRIHELQDCDANVFIALYQSILGEKVPDLIVLPRNQEDEAHNVQAVID
  SLALDYLQVSLSHITGENIVKGDNESIRNLLEIFDGLLDYLTEHISESSPNKSETEQYSKDSHGEEAGED
  LERTEEAKWRNASFMRCSFSSDTLGPTWDEDEAESTGEIIRLGDTAHTFSQRSNGAQNSKDLRSRKASAS
  PGVEPPEEMLNPGPLGFLSQNGPPCEAASETPPMSMVPSARKLGEPIRAAIPLHPPYHPSEPRAPCPIGK
  EYLWSSRYLSTPTSGEHMAPSVEPDDAFLTSTLFKDDDQETYLPKPEATRTRKPSKGERDENRAAIPSEH
  LPFSQKARKPLTEQELHAMSEKLSQRLSELDWMLKTALGDRGTGETDGNDGDGGGEEVRSGNEEMLSQHS
  DSVMEYGPKKPRPGFSTCRKAPYRSHSLSPSSVNKHRQLEKEKKRQHKSKGTDSCHFQAKALTEAFEREL
  RKHKVQENVGLRGIREEEEEEEETGKSYREVVPKGTSKRSQVQKTYSRKTAAPSPKGDGRLKSSKASPMK
  VSEHSLLSLMLEQFPFLYVSDPTLTKMWKQQMAQVEQLKREAQRENRSKKKLQDEIEEALRRHDLLTALV
  KKEYDHNKRLQDFRDRIQRQRLTQSKIKENRHQSVRARKYYDDYRVQLRAKMMKMRTREEMIFKKLFEEG
  LQIQKQRLRDLRNYAKEKRNEEKRQHQNELDSMENHYKDQFSLLAEAISQERQELKVRQKFQAQTLHKVK
  RELRAKMEKEIQQLQHMITQNDDDAFFRELEAERFKARLQLASFQYSKNPFPRGQTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177088
• ORF Size: 2481 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177088.3, NP_796062.2
• RefSeq Size: 2737 bp
• RefSeq ORF: 2484 bp
• Locus ID: 320162
• UniProt ID: Q8BVV7
• Cytogenetics: 11 E1
• MW: 95.7 kDa