Usp6nl (NM_001080548) Mouse Tagged ORF Clone

CAT#: MR215810

Usp6nl (Myc-DDK-tagged) - Mouse USP6 N-terminal like (Usp6nl), transcript variant 2

Product Images

Circular map for MR215810

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Usp6nl
• Synonyms: AI316785; mKIAA0019; RNTRE; TRE2NL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215810 representing NM_001080548
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTCCGACCAGGATGTAGCACTCAAACTTGCTCAAGAACGAGCTGAAATAGTTGCTAAATATGACA
  GAGGGCGGGAAGGTGCAGAGATCGAGCCCTGGGAAGATGCTGATTATCTTGTTTACAAAGTCACCGATAG
  ATTTGGCTTTTTACATGAGGAGGAGCTCCCATACCATAATGCAGCTGCGGACCGGCAAAAGCAACTGGAA
  ATCGAAAGGACTTCCAAGTGGCTGAAAATGCTGAAAAAATGGGAGAGATACAAGAACACCGAGAAGTTTC
  ATCGACGAATTTACAAAGGAATTCCACTCCAGCTCCGAGGTGAAGTCTGGGCTCTTCTTCTTGAGATCCC
  TAAAATGAAAGAAGAAACAAGAGACCTTTATAGTAAATTAAAACACAGAGCACGGGGTTGTTCACCTGAT
  ATCAGACAAATAGACCTTGACGTGAACCGCACATTCAGGGACCATATTATGTTTAGAGACAGATACGGTG
  TTAAGCAACAGTCCTTATTCCATGTTCTCGCTGCTTATTCTATCTATAATACGGAAGTCGGGTACTGCCA
  AGGGATGAGTCAGATCACGGCCTTGCTCCTCATGTACATGAACGAGGAAGACGCCTTCTGGGCCCTGGTC
  AAGCTCTTCTCGGGCCCCAAGCACGCAATGCACGGTTTTTTTGTCCAAGGTTTTCCTAAACTCTTGAGAT
  TTCAAGAACATCATGAGAAAATTCTGAACAAATTTTTGTCCAAACTTAAGCAGCACTTGGATTCTCAAGA
  AATCTACACAAGTTTTTACACAATGAAATGGTTTTTTCAGTGTTTCCTTGATCGAACTCCCTTCAGGCTG
  AACCTCAGGATATGGGACATCTATATCTTTGAAGGAGAGCGAGTCCTTACTGCTATGTCTTACACAATCT
  TGAAACTACACAAAAAGCATCTGATGAAGTTGTCTATGGAAGAACTTGTAGAATTTCTTCAGGAGACTCT
  CGCCAAGGATTTTTTCTTCGAGGATGATTTTGTAATAGAGCAACTTCAAGTTTCTATGGCAGAACTAAAG
  CGGGCAAAGTTAGATCTCCCAGAGCCTGGTAAGGAGGATGAATATCCAAAGAAACCCTTGGGGCAGCTTC
  CACCTGAGTCTGCTTGTGTTAATCACTTGAGCAACGGACAAAGAAGTGTTGGCCGGCCCAGCCCCAAAAC
  AAGCAGCAGGAGAGAAGATGGATCGCCTCGCAAGAACCATGAGCACTCTCCTGTCCATCACAGTAGAAAC
  GGCACACCAGAAAGAGCCGGCCAGTCACGGCGGAAGTCCGTGGATGAGGGGAGTAAAAATCTCAAGCATG
  AGGCAGAGTCTCAGAGAAAACCTTCACCGGGTATGCAGGACAGTTCCAGACACTATAACCATGCAGCTGC
  TAACCAAAATAGCAATGCAATTTCAAACGTCAGGAAGGAATTTATGCCCAAGTGGAGAAAACCATCAGAT
  GCCTCAGCTATTGAAAGAACTACTAAATATGCCGTCGAAGGCAAAAGTCACTCAGCTCTTCCCGCTCTTC
  CCGTCGCCATCCCAGGCTCTGCTGAGACCCGATTGCCAAACTCAAGGCAGAAGATGAAGGCTTTGGATGG
  GGGTGAAGGGAAGCGGGGTTCTAACGCTTCCCAGTATGACAACGTGCCTGGGGGTGAGAGTGAGCATGGG
  GCATCTGCGGAAGAGGGACCAGAGCGGACTCACCCTCACAGTCCAAGGAAGCACCCGGAGCCAAGTCCCA
  GTCCTCCAAAAGTACCCAACAAGTTTACGTTTAAAGTGCAGCCTCCCAGCCATGTACGATACCCACCCCA
  GCTACCAGAGGAGGACCACAGAGCTGCATATCCACCCTCCTACAGCAACCCCCCTGTCTACCATGGAAAT
  TCTCCAAAACACGTCCCTACTGCTCACAGTGGTTTTGTGTCTACACAGATCAGCCCCAGGCCTCAAATTA
  ACCCTTCCAGGAGACCTTACGGTTCCTCTCTTTCAGTTGACACTTCTCCAGAGAAAGCCTACAGCCGCCC
  AACTCCTGTTGTTCTGCCCTCTAGTCGAATTGAAGTCCTTCCCATTGACATGGGTGCCAGGGGCTACGGC
  AGTTCGGGGTCACCAAAGAATGGACAGTTCATCCTTCCTCCGGTGGACTATTTGCCAGAGAACAGAAAAT
  GGTCAGAAGTTAGTTACACATACAGACCGGAGATGCATGGCCAATCATGGACTCGGGATGCTCACCGTAG
  CCACTTAAGCAATTTACCAAACTATGCAGCCTTTCAGCACATACCGTTTCAGGCCCATGGCCTTCCAGAA
  GTGTCTGTAGACAGTCCAGTGAGGTATAAGATGTCTGCAGCTGTGGAAGATGCCAGTCCTCCTGGGTATC
  CATATGCAGGGCCCTCACCTTCCGCACATCACTACAGAAATGGAGAGGGACTTTCTGTGCAGGAATCAGT
  GTTGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215810 representing NM_001080548
  Red=Cloning site Green=Tags(s)
  
  MNSDQDVALKLAQERAEIVAKYDRGREGAEIEPWEDADYLVYKVTDRFGFLHEEELPYHNAAADRQKQLE
  IERTSKWLKMLKKWERYKNTEKFHRRIYKGIPLQLRGEVWALLLEIPKMKEETRDLYSKLKHRARGCSPD
  IRQIDLDVNRTFRDHIMFRDRYGVKQQSLFHVLAAYSIYNTEVGYCQGMSQITALLLMYMNEEDAFWALV
  KLFSGPKHAMHGFFVQGFPKLLRFQEHHEKILNKFLSKLKQHLDSQEIYTSFYTMKWFFQCFLDRTPFRL
  NLRIWDIYIFEGERVLTAMSYTILKLHKKHLMKLSMEELVEFLQETLAKDFFFEDDFVIEQLQVSMAELK
  RAKLDLPEPGKEDEYPKKPLGQLPPESACVNHLSNGQRSVGRPSPKTSSRREDGSPRKNHEHSPVHHSRN
  GTPERAGQSRRKSVDEGSKNLKHEAESQRKPSPGMQDSSRHYNHAAANQNSNAISNVRKEFMPKWRKPSD
  ASAIERTTKYAVEGKSHSALPALPVAIPGSAETRLPNSRQKMKALDGGEGKRGSNASQYDNVPGGESEHG
  ASAEEGPERTHPHSPRKHPEPSPSPPKVPNKFTFKVQPPSHVRYPPQLPEEDHRAAYPPSYSNPPVYHGN
  SPKHVPTAHSGFVSTQISPRPQINPSRRPYGSSLSVDTSPEKAYSRPTPVVLPSSRIEVLPIDMGARGYG
  SSGSPKNGQFILPPVDYLPENRKWSEVSYTYRPEMHGQSWTRDAHRSHLSNLPNYAAFQHIPFQAHGLPE
  VSVDSPVRYKMSAAVEDASPPGYPYAGPSPSAHHYRNGEGLSVQESVLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080548
• ORF Size: 2457 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080548.1, NP_001074017.1
• RefSeq Size: 4824 bp
• RefSeq ORF: 2460 bp
• Locus ID: 98910
• UniProt ID: Q80XC3
• Cytogenetics: 2 A1
• MW: 94 kDa
• Gene Summary: Acts as a GTPase-activating protein for RAB5A and RAB43. Involved in receptor trafficking. In complex with EPS8 inhibits internalization of EGFR. Involved in retrograde transport from the endocytic pathway to the Golgi apparatus. Involved in the transport of Shiga toxin from early and recycling endosomes to the trans-Golgi network. Required for structural integrity of the Golgi complex (By similarity).[UniProtKB/Swiss-Prot Function]