Lingo2 (NM_001166001) Mouse Tagged ORF Clone

CAT#: MR215769

Lingo2 (Myc-DDK-tagged) - Mouse leucine rich repeat and Ig domain containing 2 (Lingo2), transcript variant 4

Product Images

Circular map for MR215769

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lingo2
• Synonyms: B230217C06Rik; Lern3; Lrrn6c
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215769 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCACACGGCTATACCATGCTGGCAGCCATTCCTGGGTCTGGCTGTGGTGTTACTCTTAATGGGAT
  CCACCATTGGCTGTCCTGCTCGCTGTGAGTGCTCCGCCCAGAACAAATCTGTTAGCTGCCACAGAAGACG
  ATTGCTCGCGATCCCAGAAGGCATTCCCATTGAGACCAAAATCTTGGACCTGAGCAAAAATCGACTAAAG
  AGCATAAACCCTGAAGAGTTCATCTCATATCCTCTGTTGGAGGAGATAGACTTGAGCGACAACATTATTG
  CCAATGTGGAGCCTGGGGCATTTAACAATCTCTTTAACCTGCGTTCCCTCCGCCTAAAAGGCAATCGCCT
  TAAGTTGGTCCCTTTAGGAGTATTCACAGGACTGTCCAACCTCACCAAGCTTGACATTAGTGAGAATAAG
  ATTGTCATTTTGCTGGACTACATGTTCCAGGATCTGCATAACCTGAAGTCTCTAGAAGTGGGGGACAATG
  ATTTAGTGTATATCTCACACAGGGCCTTCAGCGGACTACTTAGCTTGGAGCAGCTCACCCTGGAGAAGTG
  CAACTTGACAGCAGTACCAACAGAAGCCCTTTCCCATCTCCGCAGCCTCATCGCCCTGCATCTGAAGCAT
  CTCAATATCAACAATATGCCTGTGTATGCCTTTAAAAGATTGTTCCACCTGAAAAACCTAGAGATCGACT
  ATTGGCCTTTGTTGGATTTGATGCCAGCCAACAGCCTCTATGGTCTCAACCTCACGTCCCTTTCAATCAC
  CAACACCAACCTGTCCACTGTCCCCTTCCTCGCCTTTAAACACCTTGTATACCTGACCCACCTTAACCTC
  TCCTACAATCCCATCAGCACTATTGAAGCTGGCATGTTCTCTGACCTGATCCGCCTACAGGAGCTTCATA
  TAGTGGGGGCCCAGCTCCGCACTATTGAGCCTCACTCCTTCCAAGGGCTCCGCTTCCTCCGTGTGCTCAA
  TGTATCTCAGAACCTGCTGGAAACATTGGAAGAGAACGTCTTCTCCTCCCCTAGGGCTTTGGAGGTCCTG
  AGCATTAACAACAACCCACTAGCCTGTGACTGCCGACTCCTCTGGCTCCTGCAGCGACAACCCAACCTGC
  AGTTTGGGGGCCAGCAGCCCATGTGTGCTGGGCCAGACACCATCCGTGAGAGATCATTTAAGGATTTCCA
  TAGCACTGCTCTTTCTTTTTATTTTACCTGCAAAAAACCCAAAATCCGTGAAAAGAAGTTACAGCATCTC
  CTCGTGGATGAAGGGCAAACGGTCCAGCTGGAGTGCAACGCTGATGGAGACCCGCAGCCCGTGATTTCCT
  GGGTGACACCTCGAAGGCGTTTTATCACCACCAAGTCCAACGGAAGGGCCACTGTGTTGGGTGATGGCAC
  CTTGGAAATCCGTTTTGCCCAGGATCAAGACAGTGGGATGTATGTTTGCATCGCGAGCAACGCTGCTGGG
  AACGATACCTTCACAGCATCTCTCACTGTGAAGGGATTCACGTCAGACCGCTTCCTTTACGCAAACAGGA
  CCCCTATGTACATGACTGACTCCAACGACACCGTTTCCAACGGCACTAATGCCAATACTTTCTCCCTGGA
  CCTTAAAACAATACTGGTATCTACAGCCATGGGCTGTTTCACATTCCTGGGAGTGGTTTTATTTTGTTTT
  CTCCTTCTTTTTGTGTGGAGCCGAGGGAAAGGCAAGCACAAAAACAGCATTGACCTTGAGTATGTGCCCC
  GAAAAAACAATGGTGCTGTTGTGGAAGGGGAGGTGGCTGGCCCCAGGAGGTTCAACATGAAAATGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215769 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLHTAIPCWQPFLGLAVVLLLMGSTIGCPARCECSAQNKSVSCHRRRLLAIPEGIPIETKILDLSKNRLK
  SINPEEFISYPLLEEIDLSDNIIANVEPGAFNNLFNLRSLRLKGNRLKLVPLGVFTGLSNLTKLDISENK
  IVILLDYMFQDLHNLKSLEVGDNDLVYISHRAFSGLLSLEQLTLEKCNLTAVPTEALSHLRSLIALHLKH
  LNINNMPVYAFKRLFHLKNLEIDYWPLLDLMPANSLYGLNLTSLSITNTNLSTVPFLAFKHLVYLTHLNL
  SYNPISTIEAGMFSDLIRLQELHIVGAQLRTIEPHSFQGLRFLRVLNVSQNLLETLEENVFSSPRALEVL
  SINNNPLACDCRLLWLLQRQPNLQFGGQQPMCAGPDTIRERSFKDFHSTALSFYFTCKKPKIREKKLQHL
  LVDEGQTVQLECNADGDPQPVISWVTPRRRFITTKSNGRATVLGDGTLEIRFAQDQDSGMYVCIASNAAG
  NDTFTASLTVKGFTSDRFLYANRTPMYMTDSNDTVSNGTNANTFSLDLKTILVSTAMGCFTFLGVVLFCF
  LLLFVWSRGKGKHKNSIDLEYVPRKNNGAVVEGEVAGPRRFNMKMI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166001
• ORF Size: 1821 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166001.1, NP_001159473.1
• RefSeq Size: 3562 bp
• RefSeq ORF: 1821 bp
• Locus ID: 242384
• UniProt ID: Q3URE9
• Cytogenetics: 4 A5
• MW: 68.1 kDa