Loxl2 (NM_033325) Mouse Tagged ORF Clone

CAT#: MR215666

Loxl2 (Myc-DDK-tagged) - Mouse lysyl oxidase-like 2 (Loxl2)

Product Images

Circular map for MR215666

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Loxl2
• Synonyms: 1110004B06Rik; 4930526G11Rik; 9430067E15Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215666 representing NM_033325
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTCCATTTTGGCTCCTGCCTCTCCGGCTGTTTGGCTCTGCTTGTCTTGCTGCCTTCCCTGAGCC
  TAGCACAGTACGAGGGCTGGCCCTACCAGCTCCAGTACCCTGAGTACTTCCAGCAGCCCGCTCCTGAGCA
  CCATCAGCGGCAGGTGCCCTCCGATGTGGTCAAGATCCAGGTCCGCCTGGCGGGCCAGAAGAGGAAGCAC
  AATGAGGGCCGCGTGGAGGTCTACTACGAAGGCCAGTGGGGCACGGTGTGCGACGATGACTTCTCGATCC
  ATGCCGCCCATGTGGTCTGCCGGCAAGTGGGCTATGTAGAGGCCAAGTCCTGGGCTGCCAGCTCCTCCTA
  CGGTCCAGGCGAAGGCCCCATCTGGTTGGACAATATCTACTGTACTGGCAAAGAGTCGACCCTGGCATCT
  TGCTCCTCCAATGGCTGGGGTGTCACTGACTGCAAGCACACTGAAGACGTTGGAGTGGTGTGTAGTGAGA
  AAAGAATTCCTGGCTTCAAATTTGACAATTCGTTGATCAACCAAATAGAGAGCCTAAATATACAGGTGGA
  AGACATCCGGATTCGGCCCATCCTTTCTGCCTTTCGCCATCGCAAGCCTGTGACAGAGGGCTACGTGGAG
  GTGAAGGAGGGCAAGGCTTGGAAGCAGATCTGCAACAAACACTGGACAGCCAAGAATTCCCACGTGGTCT
  GTGGCATGTTCGGCTTCCCTGCAGAGAAGACCTACAACCCCAAAGCCTATAAAACCTTTGCCTCGCGGAG
  GAAGCTGCGTTACTGGAAGTTTTCTATGAACTGCACGGGCACTGAAGCGCATATCTCCAGCTGCAAGCTG
  GGCCCTTCCGTGACCCGGGACCCTGTGAAGAACGCCACCTGTGAGAACGGGCAGCCAGCTGTGGTCAGTT
  GTGTGCCTAGCCAGATCTTCAGCCCCGATGGACCCTCAAGGTTCCGGAAAGCCTACAAGCCAGAGCAACC
  CTTGGTGCGCCTGAGAGGTGGAGCCCAGGTCGGGGAGGGCCGAGTGGAGGTGCTGAAGAATGGAGAATGG
  GGAACCATCTGCGATGACAAGTGGGACCTGGTATCTGCCAGTGTGGTCTGCCGAGAGCTGGGCTTTGGGA
  CCGCTAAAGAGGCCATCACAGGCTCCAGACTAGGGCAAGGGATTGGGCCCATCCATCTCAATGAAGTCCA
  GTGCACAGGGACTGAGAAGTCCATCATAGACTGCAAATTCAACACAGAGTCTCAAGGCTGCAACCATGAA
  GAAGATGCCGGGGTGCGATGCAACATCCCCATCATGGGTTTCCAGAAAAAGGTGCGCCTGAATGGAGGCC
  GCAATCCTTATGAGGGCCGAGTGGAGGTGCTAACAGAGAGAAATGGGTCCCTTGTTTGGGGGACTGTATG
  CGGCCAGAACTGGGGCATTGTGGAAGCCATGGTGGTCTGCCGGCAGCTAGGCCTGGGCTTTGCCAGCAAT
  GCCTTTCAGGAGACCTGGTACTGGCATGGAAATATCTTCGCCAACAACGTGGTCATGAGTGGAGTGAAGT
  GCTCAGGAACGGAGCTGTCCCTAGCACACTGCCGCCATGACGAGGAGGTGGCCTGCCCCGAGGGCGGGGT
  GCGGTTTGGTGCTGGAGTCGCCTGCTCGGAAACTGCACCTGACCTGGTGCTTAATGCTGAGATTGTCCAG
  CAGACTGCCTACCTGGAGGACAGGCCCATGTCCTTGCTGCAGTGTGCCATGGAGGAGAACTGCCTCTCCG
  CCTCCGCTGTGCACACCGACCCCACCAGAGGCCACCGGCGCCTTTTACGCTTCTCCTCCCAGATCCACAA
  CAATGGCCAGTCTGACTTCCGCCCCAAGAATGGCCGCCATGCGTGGATTTGGCACGACTGCCACAGGCAC
  TACCACAGCATGGAAGTCTTCACTTACTATGACCTGCTGAGCCTCAACGGCACCAAGGTGGCTGAGGGCC
  ACAAGGCCAGCTTCTGCCTGGAGGACACTGAGTGTGAGGGAGACATTCAGAAGAGTTACGAGTGTGCCAA
  CTTTGGAGAACAAGGCATCACCATGGGCTGCTGGGACATGTACCGTCATGACATTGACTGCCAGTGGATA
  GACATCACCGATGTGCCCCCTGGAGACTACCTGTTCCAGGTTGTCATTAACCCCAACTATGAAGTGCCAG
  AATCAGATTTCTCTAACAACATCATGAAGTGCAGGAGCCGCTATGATGGCTACCGCATCTGGATGTACAA
  CTGTCACGTAGGTGGAGCCTTCAGTGAGGAGACAGAACAGAAGTTCGAACACTTCAGTGGACTTCTAAAT
  AACCAGCTCTCTGTACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215666 representing NM_033325
  Red=Cloning site Green=Tags(s)
  
  MELHFGSCLSGCLALLVLLPSLSLAQYEGWPYQLQYPEYFQQPAPEHHQRQVPSDVVKIQVRLAGQKRKH
  NEGRVEVYYEGQWGTVCDDDFSIHAAHVVCRQVGYVEAKSWAASSSYGPGEGPIWLDNIYCTGKESTLAS
  CSSNGWGVTDCKHTEDVGVVCSEKRIPGFKFDNSLINQIESLNIQVEDIRIRPILSAFRHRKPVTEGYVE
  VKEGKAWKQICNKHWTAKNSHVVCGMFGFPAEKTYNPKAYKTFASRRKLRYWKFSMNCTGTEAHISSCKL
  GPSVTRDPVKNATCENGQPAVVSCVPSQIFSPDGPSRFRKAYKPEQPLVRLRGGAQVGEGRVEVLKNGEW
  GTICDDKWDLVSASVVCRELGFGTAKEAITGSRLGQGIGPIHLNEVQCTGTEKSIIDCKFNTESQGCNHE
  EDAGVRCNIPIMGFQKKVRLNGGRNPYEGRVEVLTERNGSLVWGTVCGQNWGIVEAMVVCRQLGLGFASN
  AFQETWYWHGNIFANNVVMSGVKCSGTELSLAHCRHDEEVACPEGGVRFGAGVACSETAPDLVLNAEIVQ
  QTAYLEDRPMSLLQCAMEENCLSASAVHTDPTRGHRRLLRFSSQIHNNGQSDFRPKNGRHAWIWHDCHRH
  YHSMEVFTYYDLLSLNGTKVAEGHKASFCLEDTECEGDIQKSYECANFGEQGITMGCWDMYRHDIDCQWI
  DITDVPPGDYLFQVVINPNYEVPESDFSNNIMKCRSRYDGYRIWMYNCHVGGAFSEETEQKFEHFSGLLN
  NQLSVQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033325
• ORF Size: 2328 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033325.2, NP_201582.2
• RefSeq Size: 5186 bp
• RefSeq ORF: 2331 bp
• Locus ID: 94352
• UniProt ID: P58022
• Cytogenetics: 14 D2
• MW: 87.5 kDa
• Gene Summary: Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine) (By similarity). Acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation (By similarity). Shows no activity against histone H3 when it is trimethylated on 'Lys-9' (H3K9me3) or 'Lys-27' (H3K27me3) or when 'Lys-4' is monomethylated (H3K4me1) or dimethylated (H3K4me2) (By similarity). Also mediates deamination of methylated TAF10, a member of the transcription factor IID (TFIID) complex, which induces release of TAF10 from promoters, leading to inhibition of TFIID-dependent transcription (By similarity). LOXL2-mediated deamination of TAF10 results in transcriptional repression of genes required for embryonic stem cell pluripotency including POU5F1/OCT4, NANOG, KLF4 and SOX2 (PubMed:25959397). Involved in epithelial to mesenchymal transition (EMT) via interaction with SNAI1 and participates in repression of E-cadherin, probably by mediating deamination of histone H3 (By similarity). During EMT, involved with SNAI1 in negatively regulating pericentromeric heterochromatin transcription (By similarity). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (By similarity). Interacts with the endoplasmic reticulum protein HSPA5 which activates the IRE1-XBP1 pathway of the unfolded protein response, leading to expression of several transcription factors involved in EMT and subsequent EMT induction (By similarity). When secreted into the extracellular matrix, promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin (By similarity). Acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding (By similarity). Acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation (PubMed:21071451).[UniProtKB/Swiss-Prot Function]