Zfp217 (NM_001033299) Mouse Tagged ORF Clone

CAT#: MR215598

Zfp217 (Myc-DDK-tagged) - Mouse zinc finger protein 217 (Zfp217), transcript variant 1

Product Images

Circular map for MR215598

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zfp217
• Synonyms: 4933431C08Rik; AW987152; Gm562; ZABC1; Znf217
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215598 representing NM_001033299
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGACCCAGTCCCTCCTCGTGTACATGGACGGGCCGGAAGTCCTCAGCAGCTCTCTAGGCTCCCAGA
  TGGAGGTGGATGATGCTGTGCCCATAAAAGGGCCGGTGGCAGTCCCCTTCCGAGCTGCTCAGGAGAAGAG
  CATGGCCGTGGCAGAGGGCCACATGCCCCTGGATTGCATGTTCTGCAGCCAGGTCTTCTCTCAGGCGGAG
  GATCTCAGTCAGCACGTGCTGCTGCAGCACCGGCCCACCCTCTGCGAGCCAGCTGTCCTGCGTGTGGAGG
  CCGAGTACCTAAGTCCCCTTGATAAAGCTCTGGAGCCAACAGAGCCAGCATTGGAGAAGAGTGGCGAAGA
  CCCCGAGGAGTTGAGCTGTGATGTGTGTGGGCAGACATTCCCAGTGGCTTTTGATGTTGAGAGCCACATG
  AAGAAGCATAAGGACTCCTTCACGTATGGGTGCAGCATGTGCGGGAGGAGATTCAAGGAGCCGTGGTTCC
  TGAAGAACCACATGCGGACACACAATGGCAAGTCTGGCACCAGGAGCAAGCTTCAGCAAGGCATGGAGAG
  TCCAGTCACCATCAATGAAGTGGTCCAGCCGCACGCCCCTGGGAGCATCTCCACGCCCTACAAGATCTGC
  ATGGTCTGCGGCTTCCTCTTCCCAAATAAGCAGAGCCTCATTGAGCACAGCAAGGTTCACGCCAAAGAAA
  CTGTCCCCAGTGCCAGCAACGTTGCCCCTGATGACCACCGAGAGGAACCCACGTCCCCGAGGGAAGAGTT
  GCTGCAGTTTTTGAACTTGAGACCCAGATCAACTGCAGGTAGTACAGTGAAGCCCATGACCTGCATACCT
  CAGCTTGACCCGTTCACCACCTACCAGGCATGGCAGTTGGCTACCAAAGGAAAAGTGGCCGTTGCCCAGG
  AAGAGGTGAAAGAGTCAGGCCAAGAAGGAAGCACAGACAATGACGACTCATGCTCAGAGAAAGAGGAACT
  AGGGGAAATATGGGTTGGGGGTAAGGCGGAAGGGTCTGGAAAGTCCAAAACAAGTAAAAGCAGTTGTCCA
  GGTCTCTCCCAAGACAAAGAGAAGCCTAGACATGCTAATAGTGAAGTGCCTTCTGGGGATAGTGACCCCA
  AGTTGTCCAGTAGCAAGGAGAAGCCCACGCACTGTTCTGAGTGCAGCAAAGCCTTCAGGACATACCACCA
  GCTCGTCCTGCACTCGAGGGTGCACAGGAAGGACAGGAGGACTGATGCCCTGTCACCCACCATGGCTGTG
  GATGCAAGGCAGCCTGGGACCTGCTCCCCAGACCTCAGCACCACTCTGGAAGACAGTGGGGCCGGGGACC
  GAGAAGGGGGCTCTGAAGACGGGTCTGAGGATGGACTCCCTGACGGGCTCCATTTGGATAAAAATGATGA
  TGGAGGAAAAGCGAAGCCCCTCCCGTCCTCGAGAGAGTGTAGTTACTGTGGCAAGTTTTTCCGTTCAAAC
  TATTACCTCAATATTCATCTCAGAACGCATACAGGTGAAAAACCATACAAATGTGAATTCTGTGAGTATG
  CCGCAGCCCAGAAGACATCTCTGAGGTACCACTTGGAGAGACATCACAAAGACAAGCAGCCGGTGGATGC
  TGCCGCTGAGTCCAAAAGTGAAGGCCGGAGCCAGGAGCCGCAGGATGCGCTACTAACGGCTGCTGACAGT
  GCGCAGACCAAAAATTTAAAGAGATTTCTTGATGGTGCCAAAGATGTTAAGGGAAGCCCACCTGCCAAGC
  AGCTTAAGGAGATGCCTTCTGTCTTCCAGAGTGTTCTCTCACCAGCACACAGCAACGATACTCAGGATTT
  CCATAAACATGCAGCTGATAGTGCTGAGAAAGCGAGGAAGAGCCCTGCCCCTACTTATCTGGACATGCAG
  AGAAAGAAAGCAGGGGAGCCTCAGGCCAGCAGCCCTGTCTGCAGACTAGAGGGGGTTGGGTCCTTAGCAC
  GGGAAGCTGGCCATAGGGAGAAGATGGATCAGGATGCTGACTACAGACATAAGCCCGGTGCTGACTGCCA
  GGACAGGCCTTTGAATCTATCCCTTGGGCCGCTCCACGCCTGTCCTGCAATCTCTTTGAGCAAGTGTCTG
  ATCCCCAGCATTGCCTGCCCCTTTTGTACTTTCAAGACCTTTTATCCGGAAGTCCTTATGATGCACCAGA
  GACTTGAGCACAGGTACAACCCTGACCCGCACAAGAACGGCAGCAGCAAGTCTGTGCTGAGGAACAGGCG
  TACCGGGTGCCCTCCGGCTTTGCTGGGAAAAGATGTCCCTCCCTTGTCTGGCCTGCACAAGCCCAAGGCC
  AAGACTGCCTTCTCACCACACTCGAAGTCCCTGCACTCAGAGAAGGCTCGGCAGGGGGCCTCGGGGCCAA
  GCAAAGCACCCCAGACTTCAGGACCAGACAACAGCACTTTAGCCCCAAGTAACCTGAAGTCACACAGGTC
  ACAACCCAATGCTGGGGGCACCAGTGCCACCAGGCAGCAGCAGTCAGAGTTGTTTCCCAAAGGTGGCGTC
  CCTGCTGCTATGGATAAGGTGAAGAGACCTGAGCCAAAACTGAAGTCCCTACCAGCCTCCCCGTCTCAGT
  CCCCCCTCAGCAGTAATAATAGCAACGGTTCTGTTGAGTATCCCGTGAAGGTTGACGGCCCATGGGCACA
  GCAAGGGAGAGACTACTACTGCCATCGGAATTCTGGCAGTGCCGCAGCAGAGTACAGTGAGCCACATCCC
  AAAAGACTCAAGTCCAGTGCGGTGTCCCTGGACACAGAGCATGCAGGGACCAATGGCAGAAGGGGCTTTG
  AGCTCCCCAAGTACCATGTGGTCAGGAGCATCACCTCCTTGCTACCACCAGAGTGTGTGCGCCCACCGCC
  TGTGCTGCCCCACAAAGCCCGTTTCCTGAGCCCTGGGGAGGTGGAGTCACCCAGTGTGTTGGCTGTGCAG
  AAGCCCTACAGTGCCTCTGGACCCCTGTATACCTGTGGACCCGTGGGACACGCAGGAGGCAGCCCAGCCC
  TTGAAGGAAAGAGGCCTGTGTCACATCAGCACCTATCTAACAGCATGCTGCAGAAGAGAAGCTATGAGAA
  TTTTATTGGAAATACACATTACCGACCAAATGACAAAAAACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215598 representing NM_001033299
  Red=Cloning site Green=Tags(s)
  
  MPTQSLLVYMDGPEVLSSSLGSQMEVDDAVPIKGPVAVPFRAAQEKSMAVAEGHMPLDCMFCSQVFSQAE
  DLSQHVLLQHRPTLCEPAVLRVEAEYLSPLDKALEPTEPALEKSGEDPEELSCDVCGQTFPVAFDVESHM
  KKHKDSFTYGCSMCGRRFKEPWFLKNHMRTHNGKSGTRSKLQQGMESPVTINEVVQPHAPGSISTPYKIC
  MVCGFLFPNKQSLIEHSKVHAKETVPSASNVAPDDHREEPTSPREELLQFLNLRPRSTAGSTVKPMTCIP
  QLDPFTTYQAWQLATKGKVAVAQEEVKESGQEGSTDNDDSCSEKEELGEIWVGGKAEGSGKSKTSKSSCP
  GLSQDKEKPRHANSEVPSGDSDPKLSSSKEKPTHCSECSKAFRTYHQLVLHSRVHRKDRRTDALSPTMAV
  DARQPGTCSPDLSTTLEDSGAGDREGGSEDGSEDGLPDGLHLDKNDDGGKAKPLPSSRECSYCGKFFRSN
  YYLNIHLRTHTGEKPYKCEFCEYAAAQKTSLRYHLERHHKDKQPVDAAAESKSEGRSQEPQDALLTAADS
  AQTKNLKRFLDGAKDVKGSPPAKQLKEMPSVFQSVLSPAHSNDTQDFHKHAADSAEKARKSPAPTYLDMQ
  RKKAGEPQASSPVCRLEGVGSLAREAGHREKMDQDADYRHKPGADCQDRPLNLSLGPLHACPAISLSKCL
  IPSIACPFCTFKTFYPEVLMMHQRLEHRYNPDPHKNGSSKSVLRNRRTGCPPALLGKDVPPLSGLHKPKA
  KTAFSPHSKSLHSEKARQGASGPSKAPQTSGPDNSTLAPSNLKSHRSQPNAGGTSATRQQQSELFPKGGV
  PAAMDKVKRPEPKLKSLPASPSQSPLSSNNSNGSVEYPVKVDGPWAQQGRDYYCHRNSGSAAAEYSEPHP
  KRLKSSAVSLDTEHAGTNGRRGFELPKYHVVRSITSLLPPECVRPPPVLPHKARFLSPGEVESPSVLAVQ
  KPYSASGPLYTCGPVGHAGGSPALEGKRPVSHQHLSNSMLQKRSYENFIGNTHYRPNDKKP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033299
• ORF Size: 3123 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033299.3, NP_001028471.1
• RefSeq Size: 5685 bp
• RefSeq ORF: 3126 bp
• Locus ID: 228913
• Cytogenetics: 2 89.91 cM
• MW: 113.6 kDa