Hrc (NM_010473) Mouse Tagged ORF Clone

CAT#: MR215279

Hrc (Myc-DDK-tagged) - Mouse histidine rich calcium binding protein (Hrc). Note: ORF is codon optimized

Product Images

Circular map for MR215279

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hrc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215279 representing NM_010473, codon optimized.
  Due to the complexity of NM_010473, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTTCCAGGGACCATGGTTGCACACATGCCTGCTTTGGGCCACCGTAGCCATCTTGCTGGTGCCCC
  CTGTGGTAACCCAGGAACTCAGGGGGGCAGGACTTGGGCTCGGAAATTGGAATAATAATGCAGGCATCCC
  TGGGTCTAGCGAAGACCTGTCTACGGAATTCGGCCACCACATTCATCGCGGATACCAAGGGGAAAAGGAC
  CGAGGGCATAGGGAAGAAGGGGAGGACTTCTCCAGGGAGTACGGGCACAGGGTCCAGGATCACCGATACC
  CTGGTCGAGAAGTCGGCGAAGAAAATGTCTCAGAGGAGGTGTTCCGCGGACATGTGCGCCAGCTCCACGG
  TCATAGAGAACACGACAATGAAGATCTGGGCGACTCCGCTGAAAATCACCTCCCCCGCCAGCGCAGCCAT
  TCCCACGAGGATGAGGATGGTATCGTCTCCTCCGAGTATCACCGACATGTCCCCAGGCATGCCCACCATG
  GACATGGAGAGGAAGATGACGATGATGATGGTGGCGAGGAGGAGGAGCGCGTCGACGTGATGGAGGACTC
  TGACGATAACGAGCACCAGGTCCACGGTCACCAAAGCCACTCCAAGGAGAGGGATGAGCTCCACCATGCT
  CATTCCCATAGACACCAAGGTCACTCAGACGACGACGATGATGATGGCGTCTCTACCGAGCACGGACATC
  AGGCTCATAGGTATCAAGACCACGAGGAGGAGGATGACGGAGATTCCGACGAAGATTCTCACACCCACAG
  AGTGCAGGGGCGAGAGGACGAGAACGACGATGAGGACGGCGATTCCGGCGAGTACCGACACCACACTCAG
  GATCACCAGGGCCACAATGAAGAGCAGGATGACGATGATGACGATGACGACGACGATGAGGATAAGGAGG
  ATTCTACAGAGCACAGACATCAAACTCAGGGACATCGCAAGGAAGAGGACGAAGATGAGTCAGACGAGGA
  CGACCATCATGTTAGCAGACACGGAAGACAGGGCTATGAGGAAGAAGAGGATGACGATGACGATGACGGT
  GACGACGACTCCACTGAGCACGTCCATCAGGCCCACCGCCATAGAGATCATGAGCATAAAGATGACGAAG
  ATGACAGTGAGGAGGACTACCACCATGTGCCTTCTCACGGAAGACAGAGCCATCAAAACGAGGAGGAAGA
  AGACGAAGCCGTGAGCACCGAGCATTGGCACCAATCCCCTCGCCATGCTCATCACGATCTCGGTCGAGAG
  TCTGAAGAGGAGGTAGCCGTGAAGTACAGCCACCACGTCGCATCTCACAGGCCACAGGGACACAATGCCG
  ATCGCGAGGAAGATTCTTTGGAGGAGCACATGAACGAAGTTCCAGGTCACCACCATCACCGGGCCTCCAG
  GGGGGATGACGAAGACATCTCCACAGAGTTCGGCCACAAGGCCCCCTCTCATCGCCTCCAGGACCAGGAC
  GAGCGGGCCCGCCAAGGCCATCGAGAGCCCGTCCAGGGGGAAATCGCTCACCAGCCTCTTCAGCCTACCG
  GACCCAGCTCCCGGGAGAGCAGGAAGGAGGGTGATCACAGCAGCCAGGAGGGCGACGAGGACCCTGAGCA
  AAGGCAGGCCCATTCAGAGGAGGAGAAAGAGGAGGAGGAGGAGGAGGAAGAGGAGGAGGAGGAGGAAAAG
  GAAGGCGGCCACAGCCTTCCGATGAGTCAGGAGGACGACGAGGAAGAAGAAAAGGACGAAAAGGAGTCCA
  AAGTTGACCGAGCCGCCGTGTCCGCCCCCCTGTCCCATCATCGAAAACAGGGGGAGGAGGAAGAAGAGGA
  GGAAGAAGAGGAGGAAGAGGAAATTCTGGAAGAAAACCTCCTGCCATTCACAATCATACCGAACCCCCTG
  GCAGGGAGAGAGGTGGCGAGAGAAGGCAGTTCTGAGGAGGAGTCCAGAGAAGTTACAGGCCAGCAAGACG
  CGCAAGAGTACGAGAATTACCAGCCAGGCTCCCTGTGCGGCTATTGTAGTTTTTGTAACCGATGTACTGA
  GTGCGAAAGCTGCCACTGCGACGAGGAGAACATGGGAGAGCATTGCGACCAGTGCCAACATTGTCAGTTT
  TGCTACCTCTGTCCCCTGGTATGTGATACTCTCTGCACTCCGGGCTCATATGTGGATTATTTTTCCAGTA
  GTTTGTATCAAGCACTGGCAGATATGCTTGAGACTCCAGAGCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215279 representing NM_010473
  Red=Cloning site Green=Tags(s)
  
  MGFQGPWLHTCLLWATVAILLVPPVVTQELRGAGLGLGNWNNNAGIPGSSEDLSTEFGHHIHRGYQGEKD
  RGHREEGEDFSREYGHRVQDHRYPGREVGEENVSEEVFRGHVRQLHGHREHDNEDLGDSAENHLPRQRSH
  SHEDEDGIVSSEYHRHVPRHAHHGHGEEDDDDDGGEEEERVDVMEDSDDNEHQVHGHQSHSKERDELHHA
  HSHRHQGHSDDDDDDGVSTEHGHQAHRYQDHEEEDDGDSDEDSHTHRVQGREDENDDEDGDSGEYRHHTQ
  DHQGHNEEQDDDDDDDDDDEDKEDSTEHRHQTQGHRKEEDEDESDEDDHHVSRHGRQGYEEEEDDDDDDG
  DDDSTEHVHQAHRHRDHEHKDDEDDSEEDYHHVPSHGRQSHQNEEEEDEAVSTEHWHQSPRHAHHDLGRE
  SEEEVAVKYSHHVASHRPQGHNADREEDSLEEHMNEVPGHHHHRASRGDDEDISTEFGHKAPSHRLQDQD
  ERARQGHREPVQGEIAHQPLQPTGPSSRESRKEGDHSSQEGDEDPEQRQAHSEEEKEEEEEEEEEEEEEK
  EGGHSLPMSQEDDEEEEKDEKESKVDRAAVSAPLSHHRKQGEEEEEEEEEEEEEILEENLLPFTIIPNPL
  AGREVAREGSSEEESREVTGQQDAQEYENYQPGSLCGYCSFCNRCTECESCHCDEENMGEHCDQCQHCQF
  CYLCPLVCDTLCTPGSYVDYFSSSLYQALADMLETPEP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010473
• ORF Size: 2214 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010473.2, NP_034603.2
• RefSeq Size: 2443 bp
• RefSeq ORF: 2217 bp
• Locus ID: 15464
• Cytogenetics: 7 29.26 cM
• MW: 85.2 kDa