Cyp2d22 (NM_019823) Mouse Tagged ORF Clone

CAT#: MR215267

Cyp2d22 (Myc-DDK-tagged) - Mouse cytochrome P450, family 2, subfamily d, polypeptide 22 (Cyp2d22), transcript variant 2

Product Images

Circular map for MR215267

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cyp2d22
• Synonyms: 2D22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215267 representing NM_019823
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACTGCCGACCGGGGCTGAACTGTGGCCCATAGCCATATTCACGGTCATCTTCCTGATTCTGGTGA
  ACCTGATGCACTGGCGCCAGCGCTGGACTGCCCACTACCCTCCAGGCCCTATGCCGTGGCCTGTGCTTGG
  GAACCTGCTGCACATGGACTTCCAGAATATGCCAGCAGGCTTCCAAAAGCTGCGGGGTCGCTATGGGGAC
  CTGTTCAGCCTACAGCTGGCCTCTGAGTCAGTGGTTGTACTAAATGGGCTGACGGCCCTGCGAGAGGCAC
  TGGTGAAACACAGCGAGGACACTGCTGACCGGCCACCGCTGCATTTCAATGACCTGCTGGGCTTTGGACC
  ACGCTCTCAAGGTATAGTCCTAGCACGGTATGGGCCTGCCTGGCGTCAGCAGCGGCGCTTCTCTGTGTCT
  ACCATGCACCACTTTGGCCTGGGCAAGAAGTCACTGGAGCAGTGGGTGACTGAGGAGGCCAGATGCCTCT
  GTGCCGCCTTCGCTGACCATACTGGACACCCTTTCAGCCCTAACACCCTATTGGACAAAGCAGTGTGTAA
  CGTGATCGCGTCCCTCCTCTATGCCTGCCGCTTTGAGTACGATGACCCACGCTTCATCAGGCTACTGGGC
  TTGTTGAAGGAAACTCTTAAGGAGGAAGCTGGATTCCTACCCATGTTCCTGAATGTGTTCCCGATGCTCC
  TGCGCATCCCGGGGCTGGTTGGCAAGGTCTTCCCTGGGAAAAGGGCCTTTGTTACCATGTTGGATGAGCT
  GCTGGCTGAACACAAGACGACCTGGGACCCTACCCAGCCACCCCGAGATTTGACTGATGCCTTCCTGGCT
  GAGGTGGAGAAGGCCAAGGGGAATCCTGAGAGCAGCTTCAATGATGAGAACCTGCGCACGGTAGTGGGTG
  ACCTGTTCTCTGCAGGGATGGTGACCACCTCAACCACACTGTCCTGGGCCCTGATGCTCATGATCCTGCA
  TCCAGATGTGCAGCGCCGAGTACAACAGGAAATCGATGAAGTCATAGGGCAGGTGCAGTGTCCAGAGATG
  GCAGACCAGGCTCGCATGCCCTACACCAATGCTGTCATTCATGAGGTGCAGCGCTTTGCAGACATTCTCC
  CTCTTGGTGTACCTCACAAGACTTCTCGTGACATTGAACTACAGGGCTTCCTTATCCCTAAGGGGACGAC
  CCTCATCACCAACCTGTCCTCCGCGCTAAAAGATGAGACTGTCTGGGAGAAGCCCCTCTGCTTCCATCCT
  GAACACTTCCTGGATGCCCAGGGCCACTTTGTGAAGCCTGAGGCCTTCATGCCATTCTCAGCAGGCCGCA
  GATCATGCCTGGGGGAGCCCCTGGCCCGCATGGAGCTCTTCCTCTTCTTCACCTGCCTCCTGCAGCGCTT
  TAGCATCTCAGTGCCCGATGGACAGCCCCAGCCCAGCGACCATGGCGTCTTTAGGGCTCTGACAACCCCA
  TGCCCCTACCAGCTCTGTGCTTTGCCCCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215267 representing NM_019823
  Red=Cloning site Green=Tags(s)
  
  MRLPTGAELWPIAIFTVIFLILVNLMHWRQRWTAHYPPGPMPWPVLGNLLHMDFQNMPAGFQKLRGRYGD
  LFSLQLASESVVVLNGLTALREALVKHSEDTADRPPLHFNDLLGFGPRSQGIVLARYGPAWRQQRRFSVS
  TMHHFGLGKKSLEQWVTEEARCLCAAFADHTGHPFSPNTLLDKAVCNVIASLLYACRFEYDDPRFIRLLG
  LLKETLKEEAGFLPMFLNVFPMLLRIPGLVGKVFPGKRAFVTMLDELLAEHKTTWDPTQPPRDLTDAFLA
  EVEKAKGNPESSFNDENLRTVVGDLFSAGMVTTSTTLSWALMLMILHPDVQRRVQQEIDEVIGQVQCPEM
  ADQARMPYTNAVIHEVQRFADILPLGVPHKTSRDIELQGFLIPKGTTLITNLSSALKDETVWEKPLCFHP
  EHFLDAQGHFVKPEAFMPFSAGRRSCLGEPLARMELFLFFTCLLQRFSISVPDGQPQPSDHGVFRALTTP
  CPYQLCALPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019823
• ORF Size: 1500 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019823.4, NP_062797.3
• RefSeq Size: 2782 bp
• RefSeq ORF: 1503 bp
• Locus ID: 56448
• Cytogenetics: 15 E1
• MW: 56.9 kDa