Rgs3 (NM_134257) Mouse Tagged ORF Clone

CAT#: MR215222

Rgs3 (Myc-DDK-tagged) - Mouse regulator of G-protein signaling 3 (Rgs3), transcript variant 2

Product Images

Circular map for MR215222

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rgs3
• Synonyms: 4930506N09Rik; C2pa; C2PA-RGS3; PDZ-RGS3; RGS3S
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215222 representing NM_134257
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCGCTTCAATGGGCTCTGCAAAGTGTGTTCAGAACGCCGCTACCGGCAGATCACCATCCGGAGGG
  GCAAAGACGGCTTTGGCTTCACCATCTGCTGTGACTCTCCGGTCCGAGTCCAGGCTGTGGATTCTGGGGG
  CCCGGCAGAGAGGGCGGGACTGCAGCAGCTGGACACAGTGCTACAACTGAATGAGAGACCCGTGGAGCAC
  TGGAAATGTGTGGAGCTGGCACATGAGATCCGGAGCTGTCCTAGCGAGATCATCCTGCTCGTGTGGCGTG
  TGGTCCCCCAGATCAAGCCGGGGCCAGATGGCGGAGTCTTGCGGCGGGCCTCCTGCAAGTCCACACATGA
  CCTCCTGTCACCCCCTAACAAGAGGGAGAAGAACTGTACTCATGGGGCCCCAGTTCGTCCTGAGCAGCGC
  CACAGCTGCCACCTGGTGTGTGACAGCTCTGATGGTCTACTGCTTGGTGGCTGGGAGCGCTACACTGAGG
  TGGGCAAGCGCAGTGGCCAGCACACCCTGCCTGCACTGTCCCGGACCACCACCCCTACTGACCCCAACTA
  CATCATCCTGGCCCCACTGAATCCTGGAAGCCAGTTGCTGCGGCCTGTGTACCAGGAGGATACAATCCCT
  GAAGAACCGGGGACTACTACTAAAGGGAAATCGTACACCGGCCTGGGCAAGAAGTCTCGGCTCATGAAGA
  CAGTGCAGACCATGAAGGGCCACAGTAACTACCAAGACTGCTCAGCCCTGAGACCGCACATCCCGCATTC
  CAGTTACGGCACCTATGTCACCCTGGCCCCTAAAGTCCTGGTGTTCCCTGTCTTTGTGCAGCCCCTAGAT
  CTCTGTAACCCTGCCCGGACTCTCCTGCTGTCGGAGGAGCTGCTGCTGTATGAGGGGAGGAACAAGACTT
  CCCAGGTGACACTGTTTGCCTACTCGGACCTGCTGCTGTTCACTAAGGAGGAGGAGCCAGGCCGCTGCGA
  CGTCCTGAGAAATCCCCTCTACCTCCAGAGCGTGAAGCTACAGGAGGGCTCTTCAGAAGACTTGAAATTC
  TGTGTGCTGTACCTGGCAGAGAAGGCAGAGTGCTTATTCACTTTGGAGGCACACTCGCAGGAGCAGAAGA
  AGAGAGTGTGCTGGTGCCTGTCGGAGAACATCGCCAAGCAGCAACAGCTGGCCGCACCACCTACAGAGAG
  GAAGATGTTTGAGACAGAGGCAGATGAGAAGGAGATGCCCCTGGTCGAGGGGAAGGGGCCAGGTGCTGAG
  GAACCCGCACCCAGCAAAAATCCCTCTCCTGGCCAGGAGCTTCCTCCAGGACAAGACCTCCCTCCCAGCA
  AAGACCCCTCTCCCAGCCAGGAGCTTCCTGCAGGACAAGATCTCCCTCCCAGCAAAGACCCCTCTCCCAG
  CCAGGAGCTTCCTGCAGGACAAGATCTCCCTCCCAGCAAAGACCCCTCTCCCAGCCAGGAGCTTCCTGTA
  GGACAAGACCTCCCACCCAGGAAGGACTCCTCAGGCCAAGAAGCTGCTCCTGGCCCAGAATCACCATCCA
  GTGAAGACATAGCAACCTGCCCTAAGCCCCCTCAAAGCCCAGAAACCTCAACAAGCAAGGACTCCCCACC
  AGGCCAGGGATCCTCCCCGACCACAGAACTCCCATCTTGCCAGGGCCTTCCTGCTGGTCAAGAATCTACT
  AGCCAGGACCCTCTGCTCAGTCAAGAGCCCCCTGTTATCCCAGAATCCTCTGCCTCCGTCCAGAAACGCC
  TACCCTCTCAGGAGTCACCCTCCAGCCTGGGCTCCCTGCCAGAGAAGGACCTTGCTGAGCAGACCATCAG
  CTCCGGGGAGCCACCAGTCGCCACTGGTGCTGTACTGCCAGCCTCTAGACCTAACTTTGTGATCCCCGAG
  GTGCGTCTGGATAATGCCTACAGCCAACTGGATGGGGCCCACGGAGGCAGCTCTGGCGAGGACGAGGATG
  CAGAAGAGGGAGAGGAGGGGGGAGAAGGGGAGGAAGATGAGGAGGACGACACCAGCGACGACAACTACGG
  GGATCGTAGTGAGGCCAAGCGCAGCAGCCTGATTGAGACTGGCCAAGGCGCCGAGGGCGGCTTCTCGTTG
  CGTGTGCAGAACTCGCTGCGGCGCCGGACGCACAGCGAGGGCAGCCTGCTGCAGGAGTCCCGGGGGCCCT
  GCTTTGCCTCTGACACCACCTTACACTGCTCTGATGGCGAGGGCGCCACATCCACCTGGGCTATCCCTTC
  ACCCCGCACCCTCAAAAAAGAACTGGGTCGTAATGGAGGCTCCATGCACCACCTTTCCCTGTTCTTCACG
  GGACACAGGAAGATGAGTGGGACTGACCTCACAGAATGTGATGAAGCTTCCCGGAAGAGAAAGAGCAAAA
  ACATAGCCAAGGACATGAAGAATAAGCTGGCCATCTTCAGGCGGCGGAACGAATCTCCCGGGGCTCAGCC
  AGCCAGCAAGACAGACAAGACAACCAAGTCCTTCAAGCCTACCTCGGAGGAAGCCCTCAAGTGGAGCGAG
  TCCCTGGAAAAGTTGCTGCTTCATAAATATGGCTTAGAAGTGTTCCAGGCCTTCCTTCGCACCGAGTTCA
  GTGAGGAGAACCTGGAGTTTTGGCTCGCTTGCGAGGACTTCAAGAAGGTCAAATCACAGTCCAAGATGGC
  AGCCAAAGCCAAGAAGATCTTTGCTGAATTCATCGCAATCCAGGCTTGCAAAGAGGTAAACCTGGACTCC
  TACACACGAGAACACACCAAGGAGAATCTGCAGAGCATCACCCGAGGCTGCTTTGACTTGGCACAGAAAC
  GTATCTTTGGGCTCATGGAGAAGGACTCTTACCCTCGCTTCCTCCGCTCTGACCTCTACCTGGACCTCAT
  TAACCAGAAGAAGATGAGTCCCCCGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215222 representing NM_134257
  Red=Cloning site Green=Tags(s)
  
  MNRFNGLCKVCSERRYRQITIRRGKDGFGFTICCDSPVRVQAVDSGGPAERAGLQQLDTVLQLNERPVEH
  WKCVELAHEIRSCPSEIILLVWRVVPQIKPGPDGGVLRRASCKSTHDLLSPPNKREKNCTHGAPVRPEQR
  HSCHLVCDSSDGLLLGGWERYTEVGKRSGQHTLPALSRTTTPTDPNYIILAPLNPGSQLLRPVYQEDTIP
  EEPGTTTKGKSYTGLGKKSRLMKTVQTMKGHSNYQDCSALRPHIPHSSYGTYVTLAPKVLVFPVFVQPLD
  LCNPARTLLLSEELLLYEGRNKTSQVTLFAYSDLLLFTKEEEPGRCDVLRNPLYLQSVKLQEGSSEDLKF
  CVLYLAEKAECLFTLEAHSQEQKKRVCWCLSENIAKQQQLAAPPTERKMFETEADEKEMPLVEGKGPGAE
  EPAPSKNPSPGQELPPGQDLPPSKDPSPSQELPAGQDLPPSKDPSPSQELPAGQDLPPSKDPSPSQELPV
  GQDLPPRKDSSGQEAAPGPESPSSEDIATCPKPPQSPETSTSKDSPPGQGSSPTTELPSCQGLPAGQEST
  SQDPLLSQEPPVIPESSASVQKRLPSQESPSSLGSLPEKDLAEQTISSGEPPVATGAVLPASRPNFVIPE
  VRLDNAYSQLDGAHGGSSGEDEDAEEGEEGGEGEEDEEDDTSDDNYGDRSEAKRSSLIETGQGAEGGFSL
  RVQNSLRRRTHSEGSLLQESRGPCFASDTTLHCSDGEGATSTWAIPSPRTLKKELGRNGGSMHHLSLFFT
  GHRKMSGTDLTECDEASRKRKSKNIAKDMKNKLAIFRRRNESPGAQPASKTDKTTKSFKPTSEEALKWSE
  SLEKLLLHKYGLEVFQAFLRTEFSEENLEFWLACEDFKKVKSQSKMAAKAKKIFAEFIAIQACKEVNLDS
  YTREHTKENLQSITRGCFDLAQKRIFGLMEKDSYPRFLRSDLYLDLINQKKMSPPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134257
• ORF Size: 2898 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134257.3, NP_599018.3
• RefSeq Size: 3751 bp
• RefSeq ORF: 2901 bp
• Locus ID: 50780
• UniProt ID: Q9DC04
• Cytogenetics: 4 33.19 cM
• MW: 106.2 kDa
• Gene Summary: Down-regulates signaling from heterotrimeric G-proteins by increasing the GTPase activity of the alpha subunits, thereby driving them into their inactive GDP-bound form. Down-regulates G-protein-mediated release of inositol phosphates and activation of MAP kinases.[UniProtKB/Swiss-Prot Function]