Ank3 (NM_009670) Mouse Tagged ORF Clone

CAT#: MR215182

Ank3 (Myc-DDK-tagged) - Mouse ankyrin 3, epithelial (Ank3), transcript variant 7

Product Images

Circular map for MR215182

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ank3
• Synonyms: 2900054D09Rik; AI314020; An; Ank; Ank-3; AnkG; Anky; Ankyrin-3; Ankyrin-G
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215182 representing NM_009670
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTGCCACACAGTGAAGATGCCATCACAGGGGACACTGACAAGTATCTCGGGCCACAGGACCTTA
  AGGAGCTAGGTGATGACTCCCTGCCAGCAGAAGGTTACGTAGGCTTCAGTCTTGGAGCCCGTTCTGCCAG
  CCTCCGCTCCTTCAGTTCGGATAGGTCCTACACCTTGAACAGAAGCTCCTACGCAAGGGACAGCATGATG
  ATAGAGGAACTTCTGGTACCATCCAAAGAGCAGCACCTGACGTTCACGAGGGAGTTTGATTCTGACTCCC
  TCAGACACTACAGTTGGGCAGCGGACACGTTAGATAATGTGAACCTGGTCTCAAGCCCGGTGCATTCTGG
  GTTTCTGGTTAGCTTTATGGTGGACGCGAGAGGGGGCTCCATGCGAGGAAGCCGCCACCACGGGATGCGG
  ATCATCATCCCTCCGCGAAAGTGTACGGCCCCCACCCGCATCACGTGCCGCCTGGTAAAGAGACATAAAC
  TGGCCAACCCACCCCCCATGGTGGAAGGAGAGGGATTAGCCAGTAGGCTGGTAGAAATGGGTCCTGCGGG
  GGCACAATTTTTAGGCCCCGTCATTGTGGAAATCCCTCATTTTGGGTCCATGAGGGGGAAGGAGAGAGAA
  CTTATCGTCCTTCGGAGCGAGAACGGAGAGACCTGGAAGGAACATCAGTTTGACAGTAAAAACGAAGACC
  TCGCGGAGCTTCTCAATGGCATGGATGAAGAACTCGACAGCCCGGAAGAGTTGGGTACAAAGCGCATCTG
  CAGAATTATCACAAAGGATTTCCCCCAGTATTTTGCCGTGGTTTCCCGGATTAAGCAGGAAAGCAACCAG
  ATCGGTCCTGAGGGTGGGATTCTGAGCAGCACCACCGTGCCCCTCGTCCAGGCCTCCTTCCCAGAGGGCG
  CCTTAACCAAGAGGATCCGTGTGGGTCTCCAGGCTCAGCCCGTGCCAGAGGAAACGGTAAAAAAAATCCT
  TGGGAACAAAGCAACATTTAGCCCAATTGTCACGGTAGAGCCGAGGAGAAGGAAGTTCCATAAGCCGATC
  ACCATGACCATTCCGGTGCCCCCGCCCTCGGGAGAAGGCGTGTCCAATGGGTACAAGGGGGATGCCACGC
  CCAACCTGCGGCTCCTCTGCAGCATCACAGGAGGCACCTCACCAGCTCAATGGGAAGACATCACAGGAAC
  AACCCCTCTGACGTTCATAAAGGATTGTGTGTCTTTCACAACCAACGTTTCAGCCAGATTCTGGCTGGCG
  GACTGCCATCAGGTGTTAGAGACCGTAGGGCTAGCCTCCCAGCTGTACAGAGAGCTGATATGCGTTCCCT
  ACATGGCCAAGTTCGTTGTGTTTGCCAAAACAAACGACCCGGTGGAGTCCTCGCTGAGGTGCTTCTGTAT
  GACAGACGACAGGGTGGACAAAACCCTGGAGCAGCAGGAGAACTTCGAGGAGGTTGCCAGAAGCAAAGAC
  ATTGAGGTTCTGGAAGGAAAGCCCATCTACGTTGATTGCTATGGAAACCTGGCCCCTCTGACCAAAGGAG
  GACAGCAGCTTGTTTTTAACTTTTATTCTTTCAAAGAAAACAGACTGCCATTTTCCATCAAGATCAGAGA
  CACCAGTCAAGAGCCCTGTGGCCGCCTGTCTTTCCTGAAGGAGCCAAAGACAACAAAGGGATTACCCCAA
  ACAGCTGTTTGCAACTTAAATATTACTCTGCCGGCACATAAAAAGGCTGAGAAGGCAGACAGACGCCAGA
  GCTTTGCCTCCCTAGCTTTACGTAAGCGCTACAGCTACTTGACTGAACCCAGCATGAGTCCGCAGAGTCC
  TTGTGAGCGGACGGATATCAGGATGGCGATAGTAGCCGATCACCTGGGACTTAGTTGGACAGAGCTGGCA
  AGGGAACTGAATTTTTCAGTGGATGAAATCAACCAAATACGTGTGGAAAATCCCAATTCTTTAATTTCTC
  AGAGCTTCATGTTATTAAAAAAGTGGGTGACCAGAGACGGAAAGAATGCCACAACTGATGCCTTAACTTC
  GGTCTTAACGAAGATTAACCGGATAGACATTGTAACTCTGCTGGAAGGACCAATATTTGATTATGGGAAT
  ATTTCAGGCACCAGAAGCTTTGCAGATGAAAACAATGTTTTCCATGACCCAGTTGATGGTTGGCAGAACG
  AGACGCCAAGTGGAAGCCTAGAGTCCCCAGCGCAAGCTCGAAGACTAACTGGTGGGTTACTGGACCGTCT
  GGATGACAGCTCTGACCAGGCTCGGGATTCTATTACCTCATACCTCACGGGAGAACCTGGGAAGATCGAA
  GCAAATGGAAACCACACAGCGGAAGTCATTCCAGAAGCAAAGGCAAAACCCTACTTCCCGGAATCCCAAA
  ACGATATAGGGAAACAGAGCATCAAGGAGAACCTGAAACCAAAAACACACGGATGTGGTCGCACTGAGGA
  ACCAGTGTCGCCCCTCACAGCCTACCAGAAATCTCTGGAAGAAACCAGCAAGCTTGTCATAGAAGACGCA
  CCTAAACCCTGTGTGCCTGTCGGCATGAAAAAGATGACCAGGACTACGGCTGACGGCAAAGCCAGGCTCA
  ACCTCCAGGAAGAAGAGGGGTCCACCAGGTCAGAGCCTAAGCAGGGAGAAGGCTATAAGGTGAAGACGAA
  GAAGGAAATCCGGAACGTGGAGAAGAAAACCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215182 representing NM_009670
  Red=Cloning site Green=Tags(s)
  
  MALPHSEDAITGDTDKYLGPQDLKELGDDSLPAEGYVGFSLGARSASLRSFSSDRSYTLNRSSYARDSMM
  IEELLVPSKEQHLTFTREFDSDSLRHYSWAADTLDNVNLVSSPVHSGFLVSFMVDARGGSMRGSRHHGMR
  IIIPPRKCTAPTRITCRLVKRHKLANPPPMVEGEGLASRLVEMGPAGAQFLGPVIVEIPHFGSMRGKERE
  LIVLRSENGETWKEHQFDSKNEDLAELLNGMDEELDSPEELGTKRICRIITKDFPQYFAVVSRIKQESNQ
  IGPEGGILSSTTVPLVQASFPEGALTKRIRVGLQAQPVPEETVKKILGNKATFSPIVTVEPRRRKFHKPI
  TMTIPVPPPSGEGVSNGYKGDATPNLRLLCSITGGTSPAQWEDITGTTPLTFIKDCVSFTTNVSARFWLA
  DCHQVLETVGLASQLYRELICVPYMAKFVVFAKTNDPVESSLRCFCMTDDRVDKTLEQQENFEEVARSKD
  IEVLEGKPIYVDCYGNLAPLTKGGQQLVFNFYSFKENRLPFSIKIRDTSQEPCGRLSFLKEPKTTKGLPQ
  TAVCNLNITLPAHKKAEKADRRQSFASLALRKRYSYLTEPSMSPQSPCERTDIRMAIVADHLGLSWTELA
  RELNFSVDEINQIRVENPNSLISQSFMLLKKWVTRDGKNATTDALTSVLTKINRIDIVTLLEGPIFDYGN
  ISGTRSFADENNVFHDPVDGWQNETPSGSLESPAQARRLTGGLLDRLDDSSDQARDSITSYLTGEPGKIE
  ANGNHTAEVIPEAKAKPYFPESQNDIGKQSIKENLKPKTHGCGRTEEPVSPLTAYQKSLEETSKLVIEDA
  PKPCVPVGMKKMTRTTADGKARLNLQEEEGSTRSEPKQGEGYKVKTKKEIRNVEKKTH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009670
• ORF Size: 2694 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009670.4, NP_033800.2
• RefSeq Size: 6966 bp
• RefSeq ORF: 2697 bp
• Locus ID: 11735
• UniProt ID: G5E8K5
• Cytogenetics: 10 36.1 cM
• MW: 100.2 kDa
• Gene Summary: This gene encodes a member of the ankyrin protein family. Ankyrins link integral membrane proteins to the spectrin-based cytoskeleton. Ankyrin family members share a protein structure which includes three independently folded domains: the N-terminal ankyrin repeat domain, the central spectrin-binding domain, and the C-terminal rod domain. This ankyrin functions as the major ankyrin in the kidney and may play a role in the polarized distribution of many integral membrane proteins to specific subcellular sites. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]