Ldah (NM_001167768) Mouse Tagged ORF Clone

CAT#: MR214866

1110057K04Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1110057K04 gene (1110057K04Rik), transcript variant 3

Product Images

Circular map for MR214866

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ldah
• Synonyms: 1110057K04Rik; mLDAH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR214866 representing NM_001167768
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCAGAAGTCGAGGAACAAATCCCTGTGCGTGAGGAGTTCTTTCTCTGTGGTGGAGTTGAAACCA
  AGATCATAAAATGTGGGCCCTGGACTAACCTCTTTGAGAAACAAGACGTCAGCAAGCCAAAACAGCTTAT
  CTTCATCATTCCCGGTAACCCAGGCTATTCCGCATTCTACGTGCCATTTGCAAAGGCCTTGTACACTTTG
  ATGAAGAGCCGCTTCCCGGTATGGATAATCTCTCATGCTGGGTTTTCGGTGACTCCCAAAGACAAGAAGG
  TTCTTGCAGCACCACAGGAAGAGTCAAATGCTCAAAAAATTGAGGACGTCTATGGTCTGAATGGTCAAAT
  AGAGCACAAAATAGCTTTTCTGAGAGCTCACGTGCCAAAGGATGTGAAGCTTATACTCATTGGCCATTCC
  GTAGGCACCTATATGACCCTTCACGTGATGAAGCGAGTCCCCGAGCTGCCTGTCGCCCACGCCTTTCTGC
  TCTTCCCAACTATCGAACGAATGTCTGAATCGCCCAACGGCAAGTTTGCCACTCCATTTTTGTGCCAGTT
  TCGATACCTGCTCTATGCTACCAGCTACTTACTTTTTAAGCCGTGTCCTGAAGTCATAAAATCTTTCATA
  ATCCAAAAGCTCATGGGACAAATGAACATTAAGCTTGAACTTCCTTTGACGGATATCCTTCAACCATTTT
  GCCTTGCTAACGCTGCCTACCTCGGGAGCCAAGAAATGGTCCAGATAGTGAAGCGAGATGATGACATCAT
  AAAGGAGTTTCTCCCTAAGGTAACTATGGGGACATTTTGGGGCAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR214866 representing NM_001167768
  Red=Cloning site Green=Tags(s)
  
  MASEVEEQIPVREEFFLCGGVETKIIKCGPWTNLFEKQDVSKPKQLIFIIPGNPGYSAFYVPFAKALYTL
  MKSRFPVWIISHAGFSVTPKDKKVLAAPQEESNAQKIEDVYGLNGQIEHKIAFLRAHVPKDVKLILIGHS
  VGTYMTLHVMKRVPELPVAHAFLLFPTIERMSESPNGKFATPFLCQFRYLLYATSYLLFKPCPEVIKSFI
  IQKLMGQMNIKLELPLTDILQPFCLANAAYLGSQEMVQIVKRDDDIIKEFLPKVTMGTFWGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001167768
• ORF Size: 816 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001167768.2
• RefSeq Size: 1206 bp
• RefSeq ORF: 819 bp
• Locus ID: 68832
• Cytogenetics: 12 A1.1
• MW: 31.3 kDa
• Gene Summary: Serine lipid hydrolase associated with lipid droplets. Highly expressed in macrophage-rich areas in atherosclerotic lesions, suggesting that it could promote cholesterol ester turnover in macrophages.[UniProtKB/Swiss-Prot Function]