Lekr1 (NM_001166659) Mouse Tagged ORF Clone

CAT#: MR214825

Lekr1 (Myc-DDK-tagged) - Mouse leucine, glutamate and lysine rich 1 (Lekr1), transcript variant 1

Product Images

Circular map for MR214825

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lekr1
• Synonyms: EG546798; EG624866; Gm6534
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR214825 representing NM_001166659
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCGTCACATTCCCATGCATGCCTTACCTGAAGAAATCCAAAAGATGTCTCCTGAAGAAACAGTTT
  GTAAATACTGTGGCGTCAGCTATCTAATTCTTCATGAATTTAAGGCTATGGAAGAAAAACTGAAAGCAGT
  GCAAGAAGAAATGAAATTTTATCAAGGAAGTGTAGAACGTGAAAAGAAACTTCAAGAAAAGCTGCAGTCT
  CTTAGCCAGGAGTTTGAACAGTATAAAAGTGACAGCGAGTCCAAGAAGGCAAGAGTTGAAGATGCAAGCA
  TACAGTTAAAAAAGCAACAAAATGGATTTCAGAGAGTACAGAAAGAATTAGGTCATCTACAACTTGAGTT
  AAAAATTAAACAAAAACAATCCCAAGTCTTCAGTCAAAGATTGTCAGAATACAAATACTTCTGGAATAAG
  ACTCTTTTATTACTTACTTTTACTAAAAGGGAACTAACAAGTATTAAATATGAAATATATGATAATTTTC
  AAAACTGGACTTCACTGAAAGGAGAAGTTTTTCTACAAATTAAATCTATAAGTGATACAGCTTTGGCAGA
  GATAGATATGTTAAATAAAAGTCTGACAGTGTCCCAGAGAAATAAGGTATGCCTGGAAGAGGAAATGAAA
  AGTCTCAAATTGTTGTCGGATGCAGCCATATTGAGGTCTCAGCAGATGGAGGCATCCAAACAGCATGAAG
  TGAACTTGCAAGCCAGGTGCTATGAGCTGCAGAAGGAAGCACTAGATTTACAGTGTCAAGTAGAGGCACT
  TGGGTTGAGACTTCAGAAGACAGCAAAGGAGCTGGACAACTGTAAGGAGATGCTCATGAAAAAATCTAAT
  GATGCTGAAAGCTGTGAAAGAGAACTTAGAAAACTGAAGTTTGAGTCCATTATCTCTGAGTCAAGGCACA
  AAAGGCTACTTAAAGAAAAAGAAGACTCTTTAATTACTTGCCAACAACTATATAAAACCGTACAGGAAGA
  GCTGACAACAAAAGAAAGACAAGAGGAAGATATAAAACGGAGAATCAACCTCACAGAAAATGAACTAGAG
  ATGACTAAATCTCTCCTGCTTCAGACACAGGAAGAAATTTTAACACTGAAAAATGAAAGGGAATTGATGC
  TGGTTTCTCATCAGAAAAGCATCGAGCAGCTGCAGGAAACCCTGAGACAGAAGCTGCTGAGCGATGACAG
  CTGGCGGGAGAAGATTGAAGCTGAGCTCAGCAAGGAAAGAGCCCGGCATCTCATTGAGTTCCAGGATCAA
  GCTCTTCTCTTTAAGGAGGAAGCTAAACTGGAACTTGACATTGAAAAAGAAAAGCACCAGGAAATAATCC
  GGAAGTATCAGCAAGAACATGAGGACCTGCAGAGGAAGATAGGTGACCTAATCGCAGATGCTACAAAGGA
  CCTGAGGCTGGAAGTGGCCTCTCTCCAAGAAAAACTGCATGAATCCCATGCACAATACACAGAAGAGTCT
  GAATCGAAGAAAAAAGAAATTGAAGACCTGAAAAATCTGGTAGCAGAATTCCAGTGTCGTTTGAAACAGG
  AAACTGGCCGTGATGATTCAATTTCCGAAGAGTTGAAGAAGGAAATGAAACAGAAGTCAGACGAGCTGGA
  AAGAGTAAGGCTGGCCCAGACACAGCTGCTGCAGCAGTTCAGCCAGTCACAGGAAGAGAACACTTTTCTC
  CAGGAAACCGTGCGCAGAGAGTGTGAAGAACGCTTTGAACTAACGGAGGCTTTGAGTCAAGCCAAAGAAC
  AGCTCCTGGAGCTCAGGAGGAGCGGAGGAAACTTACCGTGTTCCCTCAATAAGGGCACCCTAACCTACCC
  TGCCTCGGCAGTCAGTAATCACCGCGAGCTAAGCAGTGGGAGACAGAACTGTGGACGAGGCACCGGCACG
  CCCAGCCTGCGTGGACTGCCAAAACCCACAGCTTCCCCGACCCCAGATAAGCTCAAGAAGGTCAGCAGCT
  CGGGACTGCCTGCCCTTTCCCAGGCATATCCTCCCAGGGGCCGAGCAATTTCAGTAAACGAGGCCAGGCA
  GAGGTTGACAGCCATCCTTCGCAGGAGGTTGAGTCAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR214825 representing NM_001166659
  Red=Cloning site Green=Tags(s)
  
  MDRHIPMHALPEEIQKMSPEETVCKYCGVSYLILHEFKAMEEKLKAVQEEMKFYQGSVEREKKLQEKLQS
  LSQEFEQYKSDSESKKARVEDASIQLKKQQNGFQRVQKELGHLQLELKIKQKQSQVFSQRLSEYKYFWNK
  TLLLLTFTKRELTSIKYEIYDNFQNWTSLKGEVFLQIKSISDTALAEIDMLNKSLTVSQRNKVCLEEEMK
  SLKLLSDAAILRSQQMEASKQHEVNLQARCYELQKEALDLQCQVEALGLRLQKTAKELDNCKEMLMKKSN
  DAESCERELRKLKFESIISESRHKRLLKEKEDSLITCQQLYKTVQEELTTKERQEEDIKRRINLTENELE
  MTKSLLLQTQEEILTLKNERELMLVSHQKSIEQLQETLRQKLLSDDSWREKIEAELSKERARHLIEFQDQ
  ALLFKEEAKLELDIEKEKHQEIIRKYQQEHEDLQRKIGDLIADATKDLRLEVASLQEKLHESHAQYTEES
  ESKKKEIEDLKNLVAEFQCRLKQETGRDDSISEELKKEMKQKSDELERVRLAQTQLLQQFSQSQEENTFL
  QETVRRECEERFELTEALSQAKEQLLELRRSGGNLPCSLNKGTLTYPASAVSNHRELSSGRQNCGRGTGT
  PSLRGLPKPTASPTPDKLKKVSSSGLPALSQAYPPRGRAISVNEARQRLTAILRRRLSQQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166659
• ORF Size: 2070 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166659.1, NP_001160131.1
• RefSeq Size: 2201 bp
• RefSeq ORF: 2073 bp
• Locus ID: 624866
• Cytogenetics: 3 E1
• MW: 81.1 kDa