Htra4 (NM_001081187) Mouse Tagged ORF Clone

CAT#: MR214622

Htra4 (Myc-DDK-tagged) - Mouse HtrA serine peptidase 4 (Htra4)

Product Images

Circular map for MR214622

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Htra4
• Synonyms: B430206E18Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR214622 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTTCCAGCGGTTGTGGGCTGTGCGAACACAGTTTCTCCTGCTGTGGTTGCTGCTACCAGCCGTGC
  CTGTTCCGTGGGCCGAGGCCAGGAGGTCCAGAGTCTCGCTGCCCTGTCCCGATGCCTGCGATCCCACGCG
  CTGCCCGACTCTGCCTACCTGCTCGGCCGGCTTGGCGCCGGTTCCCGATCGCTGCGGCTGCTGCCGAGTG
  TGCGCCGCGGCCGAGGGCCAGGAGTGTGGCGGGGCGCGGGGCCGGCCGTGCGCCCCAAGGCTGCGCTGCG
  GCGCCCCGTTCTCCCGGGATCCCTCGGGGGGCGCGTGGCTGGGCACCTGCGGCTGCGCGGAGGGTGCTGA
  GGATGCGGTAGTGTGCGGCAGCGATGGGCGCACCTACCCCAGCCTATGTGCGCTCCGCAAGGAGAACCGA
  GCCGCTCGCCAGCGGGGCGCGCTCCCGGCTGTGCCAGTGCAGAAGGGTGCCTGCGAGGAAGCAGGGACCA
  CAAGAGCAGGGCGGCTCCGGAGAAAGTACAACTTCATCGCCGCAGTGGTGGAGAAGGTGGCGCCGTCTGT
  GGTCCACTTGCAGCTGTTCCGCAGATCACCTCTCACCAACCAGGAAATCCCTTCCTCCAGCGGCTCTGGG
  TTCATAGTGTCTGAGGATGGGCTCATTGTCACCAATGCCCACGTCCTCACCAACCAGCAGAAGATCCAGG
  TAGAGCTCCAGAGCGGGGCCCGGTATGAAGCCACCGTCAAAGACATCGACCATAAACTGGACCTTGCACT
  GATTAAGATCGAGCCAGATACTGAGCTTCCAGTACTGCTGCTGGGCCGATCCTCTGACCTCCGGGCTGGA
  GAGTTTGTGGTAGCTTTGGGCAGCCCATTTTCTCTGCAGAACACCGTGACTGCAGGGATTGTCAGCACCA
  CACAGAGAGGCGGCAGAGAGCTGGGACTGAAGAATTCAGACATAGACTATATCCAGACGGATGCCATCAT
  TAACCATGGAAATTCTGGGGGTCCGCTGGTGAACTTGGATGGCGACGTGATTGGTATAAACACTCTGAAG
  GTGACTGCAGGGATCTCTTTTGCGATCCCCTCTGATCGGATCAGACAGTTCCTGGAAGACTATCATGAGC
  GCCAGTTGAAAGGCAAGGCCCCTTTGCAGAAGAAATACCTGGGTCTTCGAATGCTGCCTCTCACTCTGAA
  CCTCCTTCAGGAAATGAAGAGGCAAGATCCAGAGTTCCCTGATGTGAGTTCTGGAGTTTTTGTATATGAA
  GTGATTCAGGGATCGGCTGCTGCAAGCTCGGGGTTGAGAGACCATGATGTAATTGTCAGCATAAACGGGC
  AACCTGTCACCACCACAACTGATGTCATTGAAGCTGTTAAGGACAATGACTTTCTCTCCATCATTGTGCT
  TCGAGGAAGTCAAACCTTGTTTCTGACAGTCACACCTGAAATAATCAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR214622 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSFQRLWAVRTQFLLLWLLLPAVPVPWAEARRSRVSLPCPDACDPTRCPTLPTCSAGLAPVPDRCGCCRV
  CAAAEGQECGGARGRPCAPRLRCGAPFSRDPSGGAWLGTCGCAEGAEDAVVCGSDGRTYPSLCALRKENR
  AARQRGALPAVPVQKGACEEAGTTRAGRLRRKYNFIAAVVEKVAPSVVHLQLFRRSPLTNQEIPSSSGSG
  FIVSEDGLIVTNAHVLTNQQKIQVELQSGARYEATVKDIDHKLDLALIKIEPDTELPVLLLGRSSDLRAG
  EFVVALGSPFSLQNTVTAGIVSTTQRGGRELGLKNSDIDYIQTDAIINHGNSGGPLVNLDGDVIGINTLK
  VTAGISFAIPSDRIRQFLEDYHERQLKGKAPLQKKYLGLRMLPLTLNLLQEMKRQDPEFPDVSSGVFVYE
  VIQGSAAASSGLRDHDVIVSINGQPVTTTTDVIEAVKDNDFLSIIVLRGSQTLFLTVTPEIIN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081187
• ORF Size: 1452 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081187.3, NP_001074656.1
• RefSeq Size: 2181 bp
• RefSeq ORF: 1452 bp
• Locus ID: 330723
• UniProt ID: A2RT60
• Cytogenetics: 8 A2
• MW: 52 kDa
• Gene Summary: Serine protease.[UniProtKB/Swiss-Prot Function]