3110043O21Rik (NM_001081343) Mouse Tagged ORF Clone

CAT#: MR214022

3110043O21Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 3110043O21 gene (3110043O21Rik)

Product Images

Circular map for MR214022

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 3110043O21Rik
• Synonyms: AI840585; C9orf72
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR214022 representing NM_001081343
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGACTATCTGCCCCCCACCATCTCCTGCTGTTGCCAAGACAGAGATTGCTTTAAGTGGTGAATCAC
  CCTTGTTGGCGGCTACCTTTGCTTACTGGGATAATATTCTTGGTCCTAGAGTAAGGCATATTTGGGCTCC
  AAAGACAGACCAAGTGCTTCTCAGTGATGGAGAAATAACTTTTCTTGCCAACCACACTCTAAATGGAGAA
  ATTCTTCGAAATGCAGAGAGTGGGGCTATAGATGTAAAATTTTTTGTCTTATCTGAAAAAGGGGTAATTA
  TTGTTTCATTAATCTTCGACGGAAACTGGAATGGAGATCGGAGCACTTATGGACTATCAATTATACTGCC
  GCAGACAGAGCTGAGCTTCTACCTCCCACTTCACAGAGTGTGTGTTGACAGGCTAACACACATTATTCGA
  AAAGGAAGAATATGGATGCATAAGGAAAGACAAGAAAATGTCCAGAAAATTGTCTTGGAAGGCACAGAGA
  GGATGGAAGATCAGGGTCAGAGTATCATTCCCATGCTTACTGGGGAAGTCATTCCTGTAATGGAGCTGCT
  TGCATCTATGAAATCCCACAGTGTTCCTGAAGACATTGATATAGCTGATACAGTGCTCAATGATGATGAC
  ATTGGTGACAGCTGTCACGAAGGCTTTCTTCTCAATGCCATCAGCTCACACCTGCAGACCTGTGGCTGTT
  CCGTTGTAGTTGGCAGCAGTGCAGAGAAAGTAAATAAGATAGTAAGAACGCTGTGCCTTTTTCTGACACC
  AGCAGAGAGGAAATGCTCCAGGCTGTGTGAAGCAGAATCGTCCTTTAAGTACGAATCGGGACTCTTTGTG
  CAAGGCTTGCTAAAGGATGCAACAGGCAGTTTTGTCCTACCCTTCCGGCAAGTTATGTATGCCCCGTACC
  CCACCACGCACATTGATGTGGATGTCAACACTGTCAAGCAGATGCCACCGTGTCATGAACATATTTATAA
  TCAACGCAGATACATGAGGTCAGAGCTGACAGCCTTCTGGAGGGCAACTTCAGAAGAGGACATGGCGCAG
  GACACCATCATCTACACAGATGAGAGCTTCACTCCTGATTTGAATATTTTCCAAGATGTCTTACACAGAG
  ACACTCTAGTGAAAGCCTTCCTGGATCAGGTCTTCCATTTGAAGCCTGGCCTGTCTCTCAGGAGTACTTT
  CCTTGCACAGTTCCTCCTCATTCTTCACAGAAAAGCCTTGACACTAATCAAGTACATCGAGGATGATACG
  CAGAAGGGGAAAAAGCCCTTTAAGTCTCTTCGGAACCTGAAGATAGATCTTGATTTAACAGCAGAGGGCG
  ATCTTAACATAATAATGGCTCTAGCTGAGAAAATTAAGCCAGGCCTACACTCTTTCATCTTTGGGAGACC
  TTTCTACACTAGTGTACAAGAACGTGATGTTCTAATGACCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR214022 representing NM_001081343
  Red=Cloning site Green=Tags(s)
  
  MSTICPPPSPAVAKTEIALSGESPLLAATFAYWDNILGPRVRHIWAPKTDQVLLSDGEITFLANHTLNGE
  ILRNAESGAIDVKFFVLSEKGVIIVSLIFDGNWNGDRSTYGLSIILPQTELSFYLPLHRVCVDRLTHIIR
  KGRIWMHKERQENVQKIVLEGTERMEDQGQSIIPMLTGEVIPVMELLASMKSHSVPEDIDIADTVLNDDD
  IGDSCHEGFLLNAISSHLQTCGCSVVVGSSAEKVNKIVRTLCLFLTPAERKCSRLCEAESSFKYESGLFV
  QGLLKDATGSFVLPFRQVMYAPYPTTHIDVDVNTVKQMPPCHEHIYNQRRYMRSELTAFWRATSEEDMAQ
  DTIIYTDESFTPDLNIFQDVLHRDTLVKAFLDQVFHLKPGLSLRSTFLAQFLLILHRKALTLIKYIEDDT
  QKGKKPFKSLRNLKIDLDLTAEGDLNIIMALAEKIKPGLHSFIFGRPFYTSVQERDVLMTF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081343
• ORF Size: 1443 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081343.2
• RefSeq Size: 3198 bp
• RefSeq ORF: 1446 bp
• Locus ID: 73205
• UniProt ID: Q6DFW0
• Cytogenetics: 4 A5
• MW: 54.7 kDa
• Gene Summary: Component of the C9orf72-SMCR8 complex, a complex that has guanine nucleotide exchange factor (GEF) activity and regulates autophagy (PubMed:27193190, PubMed:27617292). In the complex, C9orf72 and SMCR8 probably constitute the catalytic subunits that promote the exchange of GDP to GTP, converting inactive GDP-bound RAB8A and RAB39B into their active GTP-bound form, thereby promoting autophagosome maturation (By similarity). The C9orf72-SMCR8 complex also acts as a regulator of autophagy initiation by interacting with the ATG1/ULK1 kinase complex and modulating its protein kinase activity (PubMed:27193190, PubMed:27617292). Positively regulates initiation of autophagy by regulating the RAB1A-dependent trafficking of the ATG1/ULK1 kinase complex to the phagophore which leads to autophagosome formation (By similarity). Acts as a regulator of mTORC1 signaling by promoting phosphorylation of mTORC1 substrates (PubMed:27875531). Plays a role in endosomal trafficking (PubMed:26989253). May be involved in regulating the maturation of phagosomes to lysosomes (PubMed:26989253). Regulates actin dynamics in motor neurons by inhibiting the GTP-binding activity of ARF6, leading to ARF6 inactivation (PubMed:27723745). This reduces the activity of the LIMK1 and LIMK2 kinases which are responsible for phosphorylation and inactivation of cofilin, leading to cofilin activation (PubMed:27723745). Positively regulates axon extension and axon growth cone size in spinal motor neurons (PubMed:27723745). Plays a role within the hematopoietic system in restricting inflammation and the development of autoimmunity (PubMed:27412785).[UniProtKB/Swiss-Prot Function]