Ankrd36 (NM_023816) Mouse Tagged ORF Clone

CAT#: MR213140

Ankrd36 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 36 (Ankrd36)

Product Images

Circular map for MR213140

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ankrd36
• Synonyms: 1700008J08Rik; 1700012M14Rik; Ankrd48; RP23-387E5.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR213140 representing NM_023816
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAAGATCTTCAAGGGTCACTCGCCTTGGGGCTTTCTGAGCTTTAGGCATGCTGAGAACCAAGAAC
  CTCCCAGGTACCTCACTGGGTACATGCCTGTGAAAAGGATCCACAAAGCTGCAAGCGTGGGGGACATACC
  CCAAGTGCAGAAGATGCTCGAATTTGGGGATATTGACGTGAACGTTACAGACCGGAAGAAAAGGACTGCT
  CTTCACTACGCCTGTGCTCACGGCCAGTCAGAAATGGTGAGCCTCTTGCTGTGGTATGACTGCAACATCG
  AAGCCCGAGACCGAGAGGAAAGCACAGCTCTGATCAAGGCCACACAGCGCCAGCACGAGATCTGTGTGAA
  GATTCTTCTAGAGAACGGCGCCGACTCCAACGCCGTCGATATCCATCAGAACACCTCCCTCCACTACACG
  GTCTACAACAAGGACACCACCATAGCCGCCAAGCTTCTGGCATTCAATGCAGACACCGAAGTTAAGACAA
  AGAACGGCTACACGCCGCTTATACTTGCGGTGCTAGAAAACAAACAGGAGATGGTGGAACTCTTACTGCA
  GGCTGCAGCCAACATAAACGCCCTGGATAACTGCAAGAGGTCCGCCCTTATACATGCTGTGAGAACTCAG
  TCTAAAAACATGATCAGCCTTCTTCTCCAGCAAGGCGCCAATGCATCTTTGGTGGACATCTATGGAGCTA
  CTGCACAAAGTTACGCTGTTTTTGAAACCTTTCAAGTGCTTTCCCAAGGCCCTGGTCCCAGACCTGAGCT
  AACAACAAAAGAGGCCAGACACAGTTTTGATACCAAGGATGGCCTTGTACCATGTTCACCCCGCTTCAAG
  GAAGACAACAAAAGGATGGAGGAAAACACAGATGCAGTTGACGAGCTGGGGAGCTTTGCCCACAGACCTA
  GTAACTCTGAGCCCATAGAAGAAGAAGATGAACCTAGCTTGAGTACCAAGCCTCCCGTGGTCCAGTCTCC
  CGTGCTCACCGAGTCCAACCTCTGGGCTGGCTATCCGGCAGATTGGCCTGAACCCATTAAGTTCTCCAGG
  CAGCCAGCAGTCTACAACCCAACCGAATGGAGGGAAGACATGGAATCCCGATCAGTTCCTCTAGAGAGCA
  AACAGCAGGGTGCAGCCTTGCCGGGAAAGGAGGCAAACATACAGACACAGCAGCAGCTGCAGACCAGGGC
  TTTCAAGGAGCCTGGTGCCAATGACCCTGACCTTCCATCAAGAGAGAATGCTAAGGATGAATTGGTGTCA
  CTTTTAGCCCCTCTAGAGGAAAAAAAGCCACAAATGAAAGAATTTCCAGTAACTGAAGCTGCCAAGTTCC
  AGACAGATGTCAAGCTGGAGAGAGAAGGTGTTTTAGAATTTGAAGAAAAGGGACTTGAAATTTGCCAAGA
  CAGCAATCTAGAAGCAGCTGAAAGTACTGAGTCCTATTCAATTCTAAGGTTCGCCAAACCTGATGTTAGT
  GACCCAGAGCCCACAGCAAGAAAGGATGAATATAAGCTTGATACAAAGGACGAGGATAGGTTAGAGAGGC
  ATCTTCTGGCACCCAAGGCGTCCCAGCATCAAGCCAAGCAGACGAGCCCTCCACCTGTACAACTTCAAGA
  AAGATCTCAGGGGCCAGAGATGGACACGGCTTCTGATGAGGAAGATACGTCTGGACTTTCAAAATCCGCT
  AAGGGCCAAATACTTGGGGGAAAGTTGACATTAGGAGAAAACCCGGAGGGTAAAGAAACTGTACAGTGTG
  TCATTAACAAGCCAACGCAGAAGGGTGACGAGATCTATGAAGATGTTAACGCTAATGTTTACCTCAAGGA
  AGAGCGACCACCTGATGTCTCAGGAAAAACTCACCTGACGTCGGACCCCACTACCCTGGCCTACAATATG
  TTTGATTGTGGCGACAGAGATGTGAGCGCTGCGCTGGTCTCTGTAACACTGCAGACCTCTCCCAGACAGG
  AGGAAGGTGGCCTTGGAAATGCCTTTCCATCTCCCTCGTCCTCTCAGGACGTGGACTACAGTGGCCAGTC
  ACTTACTAAGTTTCCCTTAATGAGAAATAAATTAGACTGTGAAAATAACGCATCTGAATTGGGAGAATCT
  TTTCACAAAAGAAAAGAGAAATCTTCTAAAAACATTGAAAGCAGAAAGGTAAAAGGTAAGTGCCCAGAAG
  TACGTATGGGTGAAAAGCAAGAAGGCCATGAGTTCAAGAGCCAGTCGCAAAAAACTATGAAACCAAAACG
  CACCGACTGGCAGTTAGATATCAGACACATGCTGAAGTCCAGTGATGCCAACAGGCTTTCAAACGCCAAA
  GAAAGGAGTCACGTGACCGAAGTAAAAAGTTGGGGTGATTCTGCCCTTGCAAGCGACATGTACAGGAAAA
  GTAAAGGCACACAGGACCTGTTCCTGAAGCCACTGCCCTGCAAGGGCAATTTTGAAGCCCCAGCGCGCAC
  CTTGGGAACCGCCAGTGATGCGCCCCCTGGTGGCCCAACAACTAGAGCTTTCGCGGATGAAGAACTGCGA
  GGCAGGCTATCCGACAGTGAGTTCAGGATATTGGAGGAAGAGATCCTGGCCTGGGAGAAAGTCCACCTTG
  AAAGCGAGAGTGTCTCTGAGAATCTGCTAAACAAGTGTGAGGGGATTATATCTGATGCCACAGATCAACA
  AGGGCGATATCTAGATATGGAACAGGCAACAGAAGTGGATTCGGAGGGGACATCACAGCTGGAACAAAAG
  AATCTTGACAGCAGCGAGAATACTCAGCCGTCGAATCCAGACCTCTCAAAGACGTGCCCACAAGCCCAGA
  GCGCACCTGCAGATCCAACCACGCTGATCCAGCCCCTGCCTGAGGAGGAGAGCAAGCAGACTCACCCCCC
  ATCTTTGCGTCTCAAAAAAATGTCTGAAGATCTTGAAATAAACACGAAAGATGACAAAGAATGTGCACCA
  GGACATCCAGAAGTTCCTTCAGTGAAAGCGCATGGGAGAGAGTCTCTCACTGGAGACAGGAGAGGGCAGA
  ACAGAGAGTCAAAGCAAAGCTGCCCTGAGCTGCAGCACACAGCCGCCGATGGGCTGGAAAGCATTTGTCC
  CGTGGAGGATCCACTTCCGGGTGGCTGTAGAAGTCACTTCAAGGTTGAAGAAGTAAGCAGGAAGCAGCAG
  AGTATGGACCTGGAAGTCTCAGAGCTGTCTCCTGCTGACGAGAAAGATGAACCTGCTAGGAGTGTTCCTG
  ACTTGTGTATAAAAGACGCAAAGGGCAGTGAGAAGGAAGCATGGAGCCCCCGCGAGCCTGTGGTCACACC
  GGAATTTGAGAAGTCCCCTCCAGCAGGTGCTGATCTGCTACTACTGAAGGATGGTCACCTTCTCACTAAA
  ATGGATCGGGTTGAAGGAAGGCTGACAAAGAGAGAAGCTAAAGAAAAGCACAAGACGAAATCCCAAGTAA
  ATGAAATGGAAGAAGCTAGCCATGGAAGTGAGCCTCGTGCGCCAGCCCCAGGCTGGGGTTCTGACAGCCC
  ATTGAAGCTCTGGAACACAACTCACTCCGATGAAAGACTGATAGAGCTGAAGAATAGTCACTGCGAACTG
  CTTACAAGAAAACCTGGGAAAATGGGAAATATGAGAGAACAGACAAAAGTTAAAAAAACAAAAACCTGGT
  CACAGCATAAAGAAGTGGCATGGGACAAGCTCTGCACTCTGAGGTTCCCCTTAAAACAAGATGAGAAACA
  AGAAAATGCTGAGTTAACGTTTCAACAAAAGAAGCCACAGTTACAAAGGGAAAAGGAGCAATGTGGTGGG
  AGTGTGGAGTGGACTCAGCACTCTGAAATCAGGACAGTGATCGCAGAACTGAAGGCCCTAGAAAAGGACC
  TGAGACAGTTTCACAATCTGAAAGAAAGTTGGGAGACTACATATTACAAATACCTACACATGGTCGTAAA
  GCTTCAGTTTATCGAGCAGGAGCTGATAGGGATCAAAACAGAACAGAAGAAGTGTAGACACCTGCTCGAG
  AACCAGAAGAACTTGGAAGAAGAAGTGCTCCACTTGAAAAGCTGGGTGCGGGAGAGCGAGATCCAAGCAA
  GAGATCAAAACAACATCGAAAATTTAAGAGACACCAATGATGCAGCAATGATAAGCCAGATGGATCTCAG
  AATTAAAAACATGGAATCTCAGCTAGCCACACGGGGAGCTCAGCGACGGCTCCTGGAGGAGGGATGCTTG
  GACGTCAGCACCGCCATGCAGAAGAGGGTAGCCTGGCCCAGCCCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR213140 representing NM_023816
  Red=Cloning site Green=Tags(s)
  
  MKKIFKGHSPWGFLSFRHAENQEPPRYLTGYMPVKRIHKAASVGDIPQVQKMLEFGDIDVNVTDRKKRTA
  LHYACAHGQSEMVSLLLWYDCNIEARDREESTALIKATQRQHEICVKILLENGADSNAVDIHQNTSLHYT
  VYNKDTTIAAKLLAFNADTEVKTKNGYTPLILAVLENKQEMVELLLQAAANINALDNCKRSALIHAVRTQ
  SKNMISLLLQQGANASLVDIYGATAQSYAVFETFQVLSQGPGPRPELTTKEARHSFDTKDGLVPCSPRFK
  EDNKRMEENTDAVDELGSFAHRPSNSEPIEEEDEPSLSTKPPVVQSPVLTESNLWAGYPADWPEPIKFSR
  QPAVYNPTEWREDMESRSVPLESKQQGAALPGKEANIQTQQQLQTRAFKEPGANDPDLPSRENAKDELVS
  LLAPLEEKKPQMKEFPVTEAAKFQTDVKLEREGVLEFEEKGLEICQDSNLEAAESTESYSILRFAKPDVS
  DPEPTARKDEYKLDTKDEDRLERHLLAPKASQHQAKQTSPPPVQLQERSQGPEMDTASDEEDTSGLSKSA
  KGQILGGKLTLGENPEGKETVQCVINKPTQKGDEIYEDVNANVYLKEERPPDVSGKTHLTSDPTTLAYNM
  FDCGDRDVSAALVSVTLQTSPRQEEGGLGNAFPSPSSSQDVDYSGQSLTKFPLMRNKLDCENNASELGES
  FHKRKEKSSKNIESRKVKGKCPEVRMGEKQEGHEFKSQSQKTMKPKRTDWQLDIRHMLKSSDANRLSNAK
  ERSHVTEVKSWGDSALASDMYRKSKGTQDLFLKPLPCKGNFEAPARTLGTASDAPPGGPTTRAFADEELR
  GRLSDSEFRILEEEILAWEKVHLESESVSENLLNKCEGIISDATDQQGRYLDMEQATEVDSEGTSQLEQK
  NLDSSENTQPSNPDLSKTCPQAQSAPADPTTLIQPLPEEESKQTHPPSLRLKKMSEDLEINTKDDKECAP
  GHPEVPSVKAHGRESLTGDRRGQNRESKQSCPELQHTAADGLESICPVEDPLPGGCRSHFKVEEVSRKQQ
  SMDLEVSELSPADEKDEPARSVPDLCIKDAKGSEKEAWSPREPVVTPEFEKSPPAGADLLLLKDGHLLTK
  MDRVEGRLTKREAKEKHKTKSQVNEMEEASHGSEPRAPAPGWGSDSPLKLWNTTHSDERLIELKNSHCEL
  LTRKPGKMGNMREQTKVKKTKTWSQHKEVAWDKLCTLRFPLKQDEKQENAELTFQQKKPQLQREKEQCGG
  SVEWTQHSEIRTVIAELKALEKDLRQFHNLKESWETTYYKYLHMVVKLQFIEQELIGIKTEQKKCRHLLE
  NQKNLEEEVLHLKSWVRESEIQARDQNNIENLRDTNDAAMISQMDLRIKNMESQLATRGAQRRLLEEGCL
  DVSTAMQKRVAWPSP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023816
• ORF Size: 4245 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023816.3
• RefSeq Size: 4491 bp
• RefSeq ORF: 4248 bp
• Locus ID: 76389
• Cytogenetics: 11 A1
• MW: 159.2 kDa