Tspan15 (NM_197996) Mouse Tagged ORF Clone
CAT#: MR213074
- TrueORF®
Tspan15 (Myc-DDK-tagged) - Mouse tetraspanin 15 (Tspan15)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_197996" in other vectors (4)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Tspan15 |
Synonyms | 1110036D12Rik; 2700063A19Rik; AW048364; Tm4sf15 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR213074 representing NM_197996
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCGCGGGGACTCGGAGCAGGTGCGCTACTGCGCGCGCTTCTCCTATCTTTGGCTCAAGTTCTCTC TCATCATCTACTCCACCGTGTTCTGGCTGATTGGGGGCCTGGTCCTGTCAGTGGGGATCTACGCAGAGGC AGAGCGGCAGAAATACAAAACCCTGGAGAGTGCCTTCCTGGCCCCCGCCATCATCCTCATCCTCCTGGGG GTGGTCATGTTCATCGTCTCCTTCATCGGGGTGCTGGCTTCCCTCCGGGACAACCTGTGCCTTCTGCAGT CGTTTATGTATATCCTGGGGATCTGCCTGGTCATGGAGCTTATTGGTGGCATAGTGGCTTTGATCTTCCG GAACCAGACTATTGACTTTCTGAACGACAACATCCGGAGAGGAATCGAGAATTACTACGATGATCTGGAC TTCAAGAACATCATGGACTTTGTTCAGAAGAAGTTCAAGTGCTGTGGCGGGGAGGACTACAGAGACTGGA GCAAAAACCAGTACCATGACTGCAGCGCCCCCGGGCCCCTGGCCTGCGGGGTGCCCTACACCTGCTGCAT CAGGAACACGACAGATGTTGTCAACACCATGTGTGGCTACAAAACAATCGACAAGGAGCGCCTGAATGCA CAGAACATCATTCACGTGCGGGGCTGCACCAACGCCGTGTTGATATGGTTCATGGACAACTATACCATCA TGGCGGGCCTTTTACTGGGCATCCTGCTTCCTCAGTTTCTTGGTGTGCTGCTGACCCTACTGTACATCAC CCGTGTGGAGGACATTATCTTGGAGCACTCTGTCACGGATGGATTGCTGGGACCTGGTGCCAAGTCCAGA ACGGACACAGCAGGCACTGGATGCTGCCTGTGCTATCCCGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR213074 representing NM_197996
Red=Cloning site Green=Tags(s) MPRGDSEQVRYCARFSYLWLKFSLIIYSTVFWLIGGLVLSVGIYAEAERQKYKTLESAFLAPAIILILLG VVMFIVSFIGVLASLRDNLCLLQSFMYILGICLVMELIGGIVALIFRNQTIDFLNDNIRRGIENYYDDLD FKNIMDFVQKKFKCCGGEDYRDWSKNQYHDCSAPGPLACGVPYTCCIRNTTDVVNTMCGYKTIDKERLNA QNIIHVRGCTNAVLIWFMDNYTIMAGLLLGILLPQFLGVLLTLLYITRVEDIILEHSVTDGLLGPGAKSR TDTAGTGCCLCYPD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_197996 |
ORF Size | 882 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_197996.2, NP_932113.2 |
RefSeq Size | 3605 bp |
RefSeq ORF | 885 bp |
Locus ID | 70423 |
UniProt ID | F7BWT7 |
Cytogenetics | 10 B4 |
MW | 33.5 kDa |
Gene Summary | Regulates maturation and trafficking of the transmembrane metalloprotease ADAM10 (PubMed:23035126, PubMed:26668317). Promotes ADAM10-mediated cleavage of (CDH2) (PubMed:26668317). Negatively regulates ligand-induced Notch activity probably by regulating ADAM10 activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC211065 | Tspan15 (untagged) - Mouse tetraspanin 15 (Tspan15), (10ug) |
USD 450.00 |
|
MG213074 | Tspan15 (tGFP-tagged) - Mouse tetraspanin 15 (Tspan15), (10ug) |
USD 650.00 |
|
MR213074L3 | Lenti ORF clone of Tspan15 (Myc-DDK-tagged) - Mouse tetraspanin 15 (Tspan15) |
USD 750.00 |
|
MR213074L4 | Lenti ORF clone of Tspan15 (mGFP-tagged) - Mouse tetraspanin 15 (Tspan15) |
USD 750.00 |
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