Mindy4 (NM_177883) Mouse Tagged ORF Clone

CAT#: MR212912

Fam188b (Myc-DDK-tagged) - Mouse family with sequence similarity 188, member B (Fam188b), transcript variant 2

Product Images

Circular map for MR212912

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mindy4
• Synonyms: C330043M08Rik; Fam188b; Gm1406
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR212912 representing NM_177883
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGTCTGTACGTGGAGGAGGTGGCCGCCTCCCTGGTCAGGGAGTTTCTTAGCAGAAAGGGTTTGA
  ACAAGACATTTGTGACCATGGACCAGGAGCGCCCCCGCTGTGAGCTTAGCATCAACAGCAGAAATGATCT
  CCGCAAAGTTCTGCATCTGGAGTTTCTCTACAAGGAGAACAAGGCAAAAGAAAAGCCTCTAAGAACAAAC
  CTGGAGCTCATTACCAGGTACTTTCTGGATAATGTTGGAAATACAGATAACAGTGAAAGTCAAGAAGTCC
  CAATCCCTGCAATCCCTGTTCCAAAGAAAAACAACAAGTTGCCTTTGAGACACTCAGAGACCACATTGGT
  GAATATATACGACCTTTCAGATGAAGATACGGGAAGACGGACATCATGGTCAGAAGCAGGCAAAGCCAGG
  CATGACAGTCTGGATGGGGACATACTTGGTAATTTCGTATCATCCAAAAAACCCTCACACAAAAGTAAGG
  CTGCACACGTGGACCTGGGTGACAGCCTCCCGTTGGTTCCTGCGTGGGAGAAGGTGGACCAGCTTCACTC
  ATCAGAGCCCGGTATAGATGTGAAGAAGACAATGGAGAGGACCAGGCCTAAGTCTGGCCTGATTGTCCGA
  GGCATGATGGCTGGGCCTGTCGCCAGCTCCCCTCAGGACTCTTTCCGCAAACGCTCTCTGAGAAGGTCCT
  CAGCTCTGAGCAGGAAGCTCCAAACCCCAGAGGAGATCCAGCAGCAGTCTGAGCCTTTTGTCCACACCCC
  AGCCTATCTGGGTCCACAGGAGGTCCCCGATTCCAGCAGTGACTCCGTCTCCAGAAGCCCTCTGGGCCAG
  CTGAATGAACTGTCTATAGAGAAGCCAAATGTCACTTCTAGCAGCCAAGGGCTGTCCCAGAGGGACAGAC
  CCAGGCTAAGAAGTGTTTCAGAGGACAGCCCACTGGGGTACAGCCACACAGAGGGGAATTCCAGGATGGC
  CCAGGACCAGCTGGAAAGAGCGTTCAAGCGTCAGGGCGTCCAGCCTCCATCTCTCAGGAAAAATCAGTTG
  GTGTCTGACAGGACGGATGATAAGCCAGATGCCCTGCAGCTAGAGGATGTTGAGGATGAGTTGATAAAGG
  AAGACATTGTCTTGTTTCCACCTCCATCAATGCTCAAGCTTCAGACTGTGTCAAAGCCAATTGACCTCTC
  ACTGGCTAAGGAGATAAAGACCCTTCTGTTTGGCTCTACCTTTTGCTGCTTCAGTGAAGAATGGAAACTT
  CAGAACTTTTCCTTCAATGACATTGCCTCGTTAAAATACGGCATTGTGCAGAACAAGGGTGGTCCCTGTG
  GGGTTCTGGCAGCTGTTCAAGGCTGTGTGCTTCAGAAACTCCTGTTTGAAGGAGATAACAGAACCAACTC
  TAATCTCCGGCTGCAGCCCTCAGATGCCCAGAGGACTCGCTGCCTTGCCCTGGCCATTGCCGACATCCTG
  TGGCGAGCTGGAGGCAAAGAACAAGCCGTGGTTGCACTGGCTTCAGGAACACCGCACTTCAGTCCAACAG
  GGAAATACAAAGCAGATGGAGTCTTAGAAACAGTACGGCTTTCTGGAAAACTATCGTGCCACCTTTGGCT
  TTGTTTGGGGACTATCTCCTTTCATATAAGTAAATCCTACCCCAGCTGGGGCTGGCTGGCCATCCCTGCA
  GCCCAGGCCCCCAGAATCCTGCCTGCCCTTTGGGGGTTGGGGAGAGCTGAGTGGCTTGGTGGTTTGGCGT
  GGTTTGGCATGGTTTGGCGTGGTTTGGCTCATCAGAGCTGGTTCAGTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR212912 representing NM_177883
  Red=Cloning site Green=Tags(s)
  
  MDSLYVEEVAASLVREFLSRKGLNKTFVTMDQERPRCELSINSRNDLRKVLHLEFLYKENKAKEKPLRTN
  LELITRYFLDNVGNTDNSESQEVPIPAIPVPKKNNKLPLRHSETTLVNIYDLSDEDTGRRTSWSEAGKAR
  HDSLDGDILGNFVSSKKPSHKSKAAHVDLGDSLPLVPAWEKVDQLHSSEPGIDVKKTMERTRPKSGLIVR
  GMMAGPVASSPQDSFRKRSLRRSSALSRKLQTPEEIQQQSEPFVHTPAYLGPQEVPDSSSDSVSRSPLGQ
  LNELSIEKPNVTSSSQGLSQRDRPRLRSVSEDSPLGYSHTEGNSRMAQDQLERAFKRQGVQPPSLRKNQL
  VSDRTDDKPDALQLEDVEDELIKEDIVLFPPPSMLKLQTVSKPIDLSLAKEIKTLLFGSTFCCFSEEWKL
  QNFSFNDIASLKYGIVQNKGGPCGVLAAVQGCVLQKLLFEGDNRTNSNLRLQPSDAQRTRCLALAIADIL
  WRAGGKEQAVVALASGTPHFSPTGKYKADGVLETVRLSGKLSCHLWLCLGTISFHISKSYPSWGWLAIPA
  AQAPRILPALWGLGRAEWLGGLAWFGMVWRGLAHQSWFSG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177883
• ORF Size: 1800 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177883.4, NP_808551.2
• RefSeq Size: 2211 bp
• RefSeq ORF: 1803 bp
• Locus ID: 330323
• UniProt ID: Q3UQI9
• Cytogenetics: 6 B3
• MW: 67.2 kDa
• Gene Summary: Probable hydrolase that can remove 'Lys-48'-linked conjugated ubiquitin from proteins.[UniProtKB/Swiss-Prot Function]