Notch1 (NM_008714) Mouse Tagged ORF Clone

CAT#: MR212150

Notch1 (Myc-DDK-tagged) - Mouse Notch gene homolog 1 (Drosophila) (Notch1)

Product Images

Circular map for MR212150

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Notch1
• Synonyms: 9930111A19Rik; lin-12; Mis6; N1; Tan1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR212150 representing NM_008714
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACGGCTCCTGACGCCCTTGCTCTGCCTAACGCTGCTGCCCGCGCTCGCCGCAAGAGGCTTGAGAT
  GCTCCCAGCCAAGTGGGACCTGCCTGAATGGAGGTAGGTGCGAAGTGGCCAGCGGCACTGAAGCCTGTGT
  CTGCAGCGGAGCCTTTGTGGGCCAACGATGCCAGGACTCCAATCCTTGCCTCAGCACACCGTGTAAGAAT
  GCTGGAACGTGCCACGTTGTGGACCATGGTGGCACTGTGGATTATGCCTGCAGCTGTCCCCTGGGTTTCT
  CTGGGCCCCTCTGCCTGACACCTCTGGACAACGCCTGCCTGGCCAACCCCTGCCGCAATGGGGGCACCTG
  TGACCTGCTCACTCTCACAGAGTACAAGTGCCGCTGCCCACCAGGGTGGTCAGGAAAATCATGTCAGCAG
  GCTGACCCCTGTGCCTCCAACCCCTGTGCCAATGGTGGCCAGTGCCTGCCCTTTGAGTCTTCATACATCT
  GTCGCTGCCCGCCTGGCTTCCATGGCCCCACCTGCAGGCAAGATGTTAATGAGTGCAGCCAGAACCCTGG
  GCTGTGCCGCCATGGAGGCACCTGCCACAATGAGATCGGCTCCTATCGCTGTGCCTGCCGTGCCACCCAT
  ACTGGTCCCCACTGTGAACTGCCCTATGTGCCCTGCAGCCCCTCACCCTGCCAGAATGGAGGCACCTGCC
  GTCCTACAGGGGACACCACCCACGAGTGTGCCTGCTTGCCAGGTTTTGCTGGACAGAACTGTGAAGAAAA
  TGTGGATGACTGTCCAGGAAACAACTGCAAGAATGGGGGTGCCTGTGTGGACGGCGTGAATACCTACAAT
  TGCCGCTGCCCACCGGAGTGGACGGGTCAGTACTGTACAGAGGATGTGGACGAATGTCAGCTCATGCCCA
  ATGCCTGCCAGAATGGCGGAACCTGCCACAACACACACGGCGGCTACAACTGTGTGTGTGTCAATGGGTG
  GACTGGCGAGGACTGCAGTGAGAACATTGATGACTGTGCCAGTGCCGCCTGTTTCCAGGGTGCCACTTGC
  CACGACCGTGTGGCTTCCTTCTACTGCGAATGTCCGCATGGGCGCACAGGTCTGCTGTGCCACCTCAACG
  ATGCGTGCATCAGCAACCCCTGCAACGAGGGCTCCAACTGTGACACCAACCCTGTCAACGGCAAAGCCAT
  CTGCACCTGCCCCTCGGGGTACACAGGGCCAGCCTGCAGCCAGGACGTGGATGAGTGTGCTCTGGGTGCC
  AACCCTTGTGAGCACGCAGGCAAATGCCTCAACACACTGGGTTCTTTTGAGTGCCAGTGTCTACAGGGCT
  ACACGGGACCCCGCTGTGAGATTGATGTTAATGAGTGCATCTCCAACCCATGTCAGAATGATGCCACTTG
  CCTGGACCAGATTGGGGAGTTCCAATGCATATGTATGCCAGGTTATGAAGGTGTATACTGTGAAATCAAC
  ACGGATGAGTGCGCCAGCAGCCCCTGTCTGCACAATGGCCACTGCATGGACAAGATCAATGAGTTCCAAT
  GTCAGTGCCCCAAAGGCTTCAACGGGCACCTGTGCCAGTATGATGTGGATGAGTGTGCCAGCACACCATG
  CAAGAACGGTGCCAAGTGCCTGGATGGGCCCAACACCTATACCTGCGTGTGTACAGAAGGTTACACAGGG
  ACCCACTGCGAAGTGGACATTGACGAGTGTGACCCTGACCCCTGCCACTATGGTTCCTGTAAGGATGGTG
  TGGCCACCTTTACCTGCCTGTGCCAGCCAGGCTACACAGGCCATCACTGTGAGACCAACATCAATGAGTG
  CCACAGCCAACCGTGCCGCCATGGGGGCACCTGCCAGGACCGTGACAACTCCTACCTCTGCTTATGCCTC
  AAGGGAACCACAGGGCCCAACTGTGAGATCAACCTGGATGACTGCGCCAGCAACCCCTGTGACTCTGGCA
  CCTGTCTGGACAAGATTGATGGCTACGAATGTGCCTGTGAACCAGGCTACACAGGAAGCATGTGTAACGT
  CAACATTGACGAATGTGCGGGCAGCCCCTGCCACAACGGGGGCACTTGTGAGGATGGCATCGCGGGCTTC
  ACTTGCCGCTGCCCCGAGGGCTACCATGACCCCACGTGCCTGTCCGAGGTCAACGAGTGCAACAGTAACC
  CCTGCATCCACGGAGCTTGCCGGGATGGCCTCAATGGGTACAAGTGTGACTGTGCCCCTGGGTGGAGTGG
  AACAAACTGTGACATCAACAACAACGAGTGTGAGTCCAACCCTTGTGTCAACGGTGGCACCTGCAAGGAC
  ATGACCAGTGGCTACGTATGCACCTGCCGAGAAGGCTTCAGTGGCCCTAATTGCCAGACCAACATCAACG
  AATGTGCCTCCAACCCCTGCCTGAACCAGGGGACCTGCATTGATGATGTCGCTGGATACAAGTGCAACTG
  TCCTCTGCCATATACAGGAGCCACGTGTGAGGTGGTGTTGGCCCCATGTGCTACCAGCCCCTGCAAAAAC
  AGCGGGGTATGCAAGGAGTCTGAAGACTATGAGAGTTTTTCCTGTGTCTGTCCCACAGGCTGGCAAGGTC
  AAACCTGCGAGGTTGACATCAATGAGTGTGTGAAAAGCCCATGTCGCCATGGGGCCTCCTGCCAGAACAC
  CAATGGCAGCTACCGCTGCCTCTGCCAGGCCGGCTATACAGGTCGCAACTGTGAGAGTGACATCGATGAC
  TGCCGCCCCAACCCGTGTCACAATGGGGGTTCCTGCACCGATGGCATCAACACAGCCTTCTGCGACTGCC
  TGCCCGGCTTCCAGGGTGCCTTCTGTGAGGAGGACATCAATGAATGTGCCAGCAATCCCTGCCAAAATGG
  TGCCAATTGCACTGACTGTGTGGACAGCTACACATGTACCTGCCCCGTGGGCTTCAATGGCATCCACTGC
  GAGAACAACACACCTGACTGTACTGAGAGCTCCTGCTTCAATGGTGGTACCTGTGTGGATGGTATCAACT
  CCTTCACCTGTCTGTGTCCACCTGGCTTCACGGGCAGCTACTGTCAGTATGATGTCAATGAGTGTGATTC
  ACGGCCCTGTCTGCACGGTGGTACCTGCCAAGACAGCTATGGTACTTATAAGTGTACCTGCCCACAGGGC
  TACACTGGTCTCAACTGCCAGAACCTTGTGCGCTGGTGCGACTCGGCTCCCTGCAAGAATGGTGGCAGGT
  GCTGGCAGACCAACACGCAGTACCACTGTGAGTGCCGCAGCGGCTGGACTGGCGTCAACTGCGACGTGCT
  CAGTGTGTCCTGTGAGGTGGCTGCACAGAAGCGAGGCATTGACGTCACTCTCCTGTGCCAGCATGGAGGG
  CTCTGTGTGGATGAGGGAGATAAACATTACTGCCACTGCCAGGCAGGCTACACGGGCAGCTACTGTGAGG
  ACGAGGTGGACGAGTGCTCACCTAACCCCTGCCAGAATGGAGCTACCTGCACTGACTATCTCGGCGGCTT
  TTCCTGCAAGTGTGTGGCTGGCTACCATGGGTCTAACTGCTCAGAGGAGATCAACGAGTGCCTGTCCCAG
  CCCTGCCAGAATGGGGGTACCTGCATTGATCTGACCAACTCCTACAAGTGTTCCTGCCCCCGGGGGACAC
  AGGGTGTACACTGTGAGATCAATGTTGATGACTGCCATCCCCCCCTTGACCCTGCCTCCCGAAGCCCCAA
  GTGCTTCAACAATGGCACCTGTGTGGACCAGGTGGGTGGCTATACCTGCACCTGCCCACCAGGCTTCGTC
  GGGGAGCGGTGTGAGGGTGATGTCAATGAATGTCTCTCCAACCCCTGTGACCCACGTGGCACCCAGAACT
  GTGTGCAGCGTGTTAATGACTTCCACTGCGAGTGCCGGGCTGGCCACACTGGACGCCGCTGTGAGTCAGT
  CATCAATGGCTGCAGGGGCAAACCTTGCAAGAATGGGGGTGTCTGTGCCGTGGCCTCCAACACCGCCCGT
  GGATTCATCTGTAGGTGCCCTGCGGGCTTCGAGGGTGCCACATGTGAGAATGATGCCCGCACTTGTGGCA
  GCTTACGCTGCCTCAACGGTGGTACATGCATCTCGGGCCCACGTAGTCCCACCTGCCTATGCCTGGGATC
  CTTCACCGGCCCTGAGTGCCAGTTCCCAGCCAGCAGCCCCTGTGTGGGTAGCAACCCCTGCTACAATCAG
  GGCACCTGTGAGCCCACATCCGAGAACCCTTTCTACCGCTGTCTATGCCCTGCCAAATTCAACGGGCTAC
  TGTGCCACATCCTGGACTACAGCTTCACAGGTGGCGCTGGGCGCGACATTCCCCCACCGCAGATTGAGGA
  GGCCTGTGAGCTGCCTGAGTGCCAGGTGGATGCAGGCAATAAGGTCTGCAACCTGCAGTGTAATAATCAC
  GCATGTGGCTGGGATGGTGGCGACTGCTCCCTCAACTTCAATGACCCCTGGAAGAACTGCACGCAGTCTC
  TACAGTGCTGGAAGTATTTTAGCGACGGCCACTGTGACAGCCAGTGCAACTCGGCCGGCTGCCTCTTTGA
  TGGCTTCGACTGCCAGCTCACCGAGGGACAGTGCAACCCCCTGTATGACCAGTACTGCAAGGACCACTTC
  AGTGATGGCCACTGCGACCAGGGCTGTAACAGTGCCGAATGTGAGTGGGATGGCCTAGACTGTGCTGAGC
  ATGTACCCGAGCGGCTGGCAGCCGGCACCCTGGTGCTGGTGGTGCTGCTTCCACCCGACCAGCTACGGAA
  CAACTCCTTCCACTTTCTGCGGGAGCTCAGCCACGTGCTGCACACCAACGTGGTCTTCAAGCGTGATGCG
  CAAGGCCAGCAGATGATCTTCCCGTACTATGGCCACGAGGAAGAGCTGCGCAAGCACCCAATCAAGCGCT
  CTACAGTGGGTTGGGCCACCTCTTCACTGCTTCCTGGTACCAGTGGTGGGCGCCAGCGCAGGGAGCTGGA
  CCCCATGGACATCCGTGGCTCCATTGTCTACCTGGAGATCGACAACCGGCAATGTGTGCAGTCATCCTCG
  CAGTGCTTCCAGAGTGCCACCGATGTGGCTGCCTTCCTAGGTGCTCTTGCGTCACTTGGCAGCCTCAATA
  TTCCTTACAAGATTGAGGCCGTGAAGAGTGAGCCGGTGGAGCCTCCGCTGCCCTCGCAGCTGCACCTCAT
  GTACGTGGCAGCGGCCGCCTTCGTGCTCCTGTTCTTTGTGGGCTGTGGGGTGCTGCTGTCCCGCAAGCGC
  CGGCGGCAGCATGGCCAGCTCTGGTTCCCTGAGGGTTTCAAAGTGTCAGAGGCCAGCAAGAAGAAGCGGA
  GAGAGCCCCTCGGCGAGGACTCAGTCGGCCTCAAGCCCCTGAAGAATGCCTCAGATGGTGCTCTGATGGA
  CGACAATCAGAACGAGTGGGGAGACGAAGACCTGGAGACCAAGAAGTTCCGGTTTGAGGAGCCAGTAGTT
  CTCCCTGACCTGAGTGATCAGACTGACCACAGACAGTGGACCCAGCAGCACCTGGACGCTGCTGACCTGC
  GCATGTCTGCCATGGCCCCAACACCGCCTCAGGGGGAGGTGGATGCTGACTGCATGGATGTCAATGTTCG
  AGGACCAGATGGCTTCACACCCCTCATGATTGCCTCCTGCAGTGGAGGGGGCCTTGAGACAGGCAACAGT
  GAAGAAGAAGAAGATGCACCTGCTGTCATCTCTGACTTCATCTACCAGGGCGCCAGCTTGCACAACCAGA
  CAGACCGCACCGGGGAGACCGCCTTGCACTTGGCTGCCCGATACTCTCGTTCAGATGCTGCAAAGCGCTT
  GCTGGAGGCCAGTGCAGATGCCAACATCCAGGACAACATGGGCCGTACTCCGTTACATGCAGCAGTTTCT
  GCAGATGCTCAGGGTGTCTTCCAGATCCTGCTCCGGAACAGGGCCACAGATCTGGATGCCCGAATGCATG
  ATGGCACAACTCCACTGATCCTGGCTGCGCGCCTGGCCCTGGAGGGCATGCTGGAGGACCTCATCAACTC
  ACATGCTGACGTCAATGCCGTGGATGACCTAGGCAAGTCGGCTTTGCATTGGGCGGCCGCGGTGAACAAT
  GTGGATGCTGCTGTTGTGCTCCTGAAGAACGGAGCCAACAAGGACATGCAGAACAACAAGGAGGAGACTC
  CCCTGTTCCTGGCCGCCCGTGAGGGCAGCTATGAGACTGCCAAAGTGTTGCTGGACCACTTTGCCAACCG
  GGACATCACGGATCACATGGACCGATTGCCGCGGGACATCGCACAGGAGCGTATGCACCACGATATCGTG
  CGGCTTTTGGATGAGTACAACCTGGTGCGCAGCCCACAGCTGCATGGCACTGCCCTGGGTGGCACACCCA
  CTCTGTCTCCCACACTCTGCTCGCCCAATGGCTACCTGGGCAATCTCAAGTCTGCCACACAGGGCAAGAA
  GGCCCGCAAGCCCAGCACCAAAGGGCTGGCTTGTGGTAGCAAGGAAGCTAAGGACCTCAAGGCACGGAGG
  AAGAAGTCCCAGGATGGCAAGGGCTGCCTGTTGGACAGCTCGAGCATGCTGTCGCCTGTGGACTCCCTCG
  AGTCACCCCATGGCTACTTGTCAGATGTGGCCTCGCCACCCCTCCTCCCCTCCCCATTCCAGCAGTCTCC
  ATCCATGCCTCTCAGCCACCTGCCTGGTATGCCTGACACTCACCTGGGCATCAGCCACTTGAATGTGGCA
  GCCAAGCCTGAGATGGCAGCACTGGCTGGAGGTAGCCGGTTGGCCTTTGAGCCACCCCCGCCACGCCTCT
  CCCACCTGCCTGTAGCCTCCAGTGCCAGCACAGTGCTGAGTACCAATGGCACGGGGGCTATGAATTTCAC
  CGTGGGTGCACCGGCAAGCTTGAATGGCCAGTGTGAGTGGCTTCCCCGGCTCCAGAATGGCATGGTGCCC
  AGCCAGTACAACCCACTACGGCCGGGTGTGACGCCGGGCACACTGAGCACACAGGCAGCTGGCCTCCAGC
  ATAGCATGATGGGGCCACTACACAGCAGCCTCTCCACCAATACCTTGTCCCCGATTATTTACCAGGGCCT
  GCCCAACACACGGCTGGCAACACAGCCTCACCTGGTGCAGACCCAGCAGGTGCAGCCACAGAACTTACAG
  CTCCAGCCTCAGAACCTGCAGCCACCATCACAGCCACACCTCAGTGTGAGCTCGGCAGCCAATGGGCACC
  TGGGCCGGAGCTTCTTGAGTGGGGAGCCCAGTCAGGCAGATGTACAACCGCTGGGCCCCAGCAGTCTGCC
  TGTGCACACCATTCTGCCCCAGGAAAGCCAGGCCCTGCCCACATCACTGCCATCCTCCATGGTCCCACCC
  ATGACCACTACCCAGTTCCTGACCCCTCCTTCCCAGCACAGTTACTCCTCCTCCCCTGTGGACAACACCC
  CCAGCCACCAGCTGCAGGTGCCAGAGCACCCCTTCCTCACCCCATCCCCTGAGTCCCCTGACCAGTGGTC
  CAGCTCCTCCCCGCATTCCAACATCTCTGATTGGTCCGAGGGCATCTCCAGCCCGCCCACCACCATGCCG
  TCCCAGATCACCCACATTCCAGAGGCATTTAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR212150 representing NM_008714
  Red=Cloning site Green=Tags(s)
  
  MPRLLTPLLCLTLLPALAARGLRCSQPSGTCLNGGRCEVASGTEACVCSGAFVGQRCQDSNPCLSTPCKN
  AGTCHVVDHGGTVDYACSCPLGFSGPLCLTPLDNACLANPCRNGGTCDLLTLTEYKCRCPPGWSGKSCQQ
  ADPCASNPCANGGQCLPFESSYICRCPPGFHGPTCRQDVNECSQNPGLCRHGGTCHNEIGSYRCACRATH
  TGPHCELPYVPCSPSPCQNGGTCRPTGDTTHECACLPGFAGQNCEENVDDCPGNNCKNGGACVDGVNTYN
  CRCPPEWTGQYCTEDVDECQLMPNACQNGGTCHNTHGGYNCVCVNGWTGEDCSENIDDCASAACFQGATC
  HDRVASFYCECPHGRTGLLCHLNDACISNPCNEGSNCDTNPVNGKAICTCPSGYTGPACSQDVDECALGA
  NPCEHAGKCLNTLGSFECQCLQGYTGPRCEIDVNECISNPCQNDATCLDQIGEFQCICMPGYEGVYCEIN
  TDECASSPCLHNGHCMDKINEFQCQCPKGFNGHLCQYDVDECASTPCKNGAKCLDGPNTYTCVCTEGYTG
  THCEVDIDECDPDPCHYGSCKDGVATFTCLCQPGYTGHHCETNINECHSQPCRHGGTCQDRDNSYLCLCL
  KGTTGPNCEINLDDCASNPCDSGTCLDKIDGYECACEPGYTGSMCNVNIDECAGSPCHNGGTCEDGIAGF
  TCRCPEGYHDPTCLSEVNECNSNPCIHGACRDGLNGYKCDCAPGWSGTNCDINNNECESNPCVNGGTCKD
  MTSGYVCTCREGFSGPNCQTNINECASNPCLNQGTCIDDVAGYKCNCPLPYTGATCEVVLAPCATSPCKN
  SGVCKESEDYESFSCVCPTGWQGQTCEVDINECVKSPCRHGASCQNTNGSYRCLCQAGYTGRNCESDIDD
  CRPNPCHNGGSCTDGINTAFCDCLPGFQGAFCEEDINECASNPCQNGANCTDCVDSYTCTCPVGFNGIHC
  ENNTPDCTESSCFNGGTCVDGINSFTCLCPPGFTGSYCQYDVNECDSRPCLHGGTCQDSYGTYKCTCPQG
  YTGLNCQNLVRWCDSAPCKNGGRCWQTNTQYHCECRSGWTGVNCDVLSVSCEVAAQKRGIDVTLLCQHGG
  LCVDEGDKHYCHCQAGYTGSYCEDEVDECSPNPCQNGATCTDYLGGFSCKCVAGYHGSNCSEEINECLSQ
  PCQNGGTCIDLTNSYKCSCPRGTQGVHCEINVDDCHPPLDPASRSPKCFNNGTCVDQVGGYTCTCPPGFV
  GERCEGDVNECLSNPCDPRGTQNCVQRVNDFHCECRAGHTGRRCESVINGCRGKPCKNGGVCAVASNTAR
  GFICRCPAGFEGATCENDARTCGSLRCLNGGTCISGPRSPTCLCLGSFTGPECQFPASSPCVGSNPCYNQ
  GTCEPTSENPFYRCLCPAKFNGLLCHILDYSFTGGAGRDIPPPQIEEACELPECQVDAGNKVCNLQCNNH
  ACGWDGGDCSLNFNDPWKNCTQSLQCWKYFSDGHCDSQCNSAGCLFDGFDCQLTEGQCNPLYDQYCKDHF
  SDGHCDQGCNSAECEWDGLDCAEHVPERLAAGTLVLVVLLPPDQLRNNSFHFLRELSHVLHTNVVFKRDA
  QGQQMIFPYYGHEEELRKHPIKRSTVGWATSSLLPGTSGGRQRRELDPMDIRGSIVYLEIDNRQCVQSSS
  QCFQSATDVAAFLGALASLGSLNIPYKIEAVKSEPVEPPLPSQLHLMYVAAAAFVLLFFVGCGVLLSRKR
  RRQHGQLWFPEGFKVSEASKKKRREPLGEDSVGLKPLKNASDGALMDDNQNEWGDEDLETKKFRFEEPVV
  LPDLSDQTDHRQWTQQHLDAADLRMSAMAPTPPQGEVDADCMDVNVRGPDGFTPLMIASCSGGGLETGNS
  EEEEDAPAVISDFIYQGASLHNQTDRTGETALHLAARYSRSDAAKRLLEASADANIQDNMGRTPLHAAVS
  ADAQGVFQILLRNRATDLDARMHDGTTPLILAARLALEGMLEDLINSHADVNAVDDLGKSALHWAAAVNN
  VDAAVVLLKNGANKDMQNNKEETPLFLAAREGSYETAKVLLDHFANRDITDHMDRLPRDIAQERMHHDIV
  RLLDEYNLVRSPQLHGTALGGTPTLSPTLCSPNGYLGNLKSATQGKKARKPSTKGLACGSKEAKDLKARR
  KKSQDGKGCLLDSSSMLSPVDSLESPHGYLSDVASPPLLPSPFQQSPSMPLSHLPGMPDTHLGISHLNVA
  AKPEMAALAGGSRLAFEPPPPRLSHLPVASSASTVLSTNGTGAMNFTVGAPASLNGQCEWLPRLQNGMVP
  SQYNPLRPGVTPGTLSTQAAGLQHSMMGPLHSSLSTNTLSPIIYQGLPNTRLATQPHLVQTQQVQPQNLQ
  LQPQNLQPPSQPHLSVSSAANGHLGRSFLSGEPSQADVQPLGPSSLPVHTILPQESQALPTSLPSSMVPP
  MTTTQFLTPPSQHSYSSSPVDNTPSHQLQVPEHPFLTPSPESPDQWSSSSPHSNISDWSEGISSPPTTMP
  SQITHIPEAFK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008714
• ORF Size: 7593 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008714.2, NP_032740.2
• RefSeq Size: 9497 bp
• RefSeq ORF: 7596 bp
• Locus ID: 18128
• UniProt ID: Q01705
• Cytogenetics: 2 18.91 cM
• MW: 271.3 kDa
• Gene Summary: Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4(+) and CD8(+) cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).[UniProtKB/Swiss-Prot Function]