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Heatr5a (NM_177171) Mouse Tagged ORF Clone
CAT#: MR212107
- TrueORF®
Heatr5a (Myc-DDK-tagged) - Mouse HEAT repeat containing 5A (Heatr5a)
ORF Plasmid: tGFP
"NM_177171" in other vectors (2)
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | Heatr5a |
| Synonyms | C230081H03Rik; D930036F22Rik; mKIAA1316 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR212107 representing NM_177171
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAATTGGCTCACAGCTTGCTGCTCAATGAAGAAGCATCCAATCAGCTAGGTGCCGTTCAGAAGGCTG AGTTTATTTTTGAGTGGTTGAGATACTTGGAGAAGCTCTTGCTAGCAACAAACAGGGAGGACGTCAGGGA GAAGCAGAAGACTCTTGTTGGGCAGCTCCTGTCCTTGCTGAACAGCTCCCCGGGACCCCCCACGCGCAAG CTCCTTGCTCAGGACCTGGCCATCCTTTATAGTGTTGGAGACACAGTCTCTGTTTATGAGACGATCGATA AATGTAATGATCTTATTCGGAGCAAAGATGACTCTCCAAGTTACCTTCCCACCAAACTTGCTGCTGTGGT GTGCTTGGGTTCCTTGTACAAGAAATTGGGTAGAATCCTGGCTAATGGCTTCACTGACACTGTGGTGAAT ATTCTTAAAGCGATGAAGAGTGCAGAGTCTCAAGGCCGCTATGAGATCATGCTGAGTCTGCAGAGTATAC TGACTGGGCTGGGTGCTGCCGCTGCACCGTGCCACAGGGACGTTTACAAGGCTGCCCGGTCCTGCTTAAC AGACAGATCCATGGCTGTCCGATGTGCTGCTGCCAAGTGTCTTCTTGAACTCCAGAATGAGGCCATCTTT ATGTGGAGCACAGACGTGGACAGTGTGGCCACACTATGTTTTAAGTCCTTTGAAGGTTCCAATTATGACG TGCGGATATCAGTTTCAAAACTGTTGGGTACAGTGTTGGCTAAAGCTGTAACTGCTAAGCATCCAGGAGC AGGCTCAAAGCAGAGTGCTCGTAGAGTGTCACTGGAGGAAGTTCTGGAACTGCTGGGAGCAGGGTTTCTG CGTGGGAGTTCAGGATTCCTTCGAGCCAGTGGAGATATGCTGAAAGGAAACAGTTCAGTCAGCAGAGACG TTCGAGTCGGAGTTACTCAGGCTTACGTGGTATTTGTTTCAACATTAGGAGGAGCTTGGCTAGAGAAGAA TCTTGCTGCCTTTCTTTCCCACATCCTAAGCCTTGTATCACAGTCAAATCCTAAAGCCACACAGACGCAG ATTGACGCCGTCTGCTGTCGCCGCTGTGTCTCATTCATTCTTCGAGCGACTCTAGGAGGCCTGCTTGGAG AGAAGGCGCAGATAGCTGCTGCAAAGGAGATCTGCCAGGCAGTCTGGAGGCTAAAGAAAGTTATGGATGC CGCATTGAGTGATGGTAATGTGGAGACCCGTCTCAGTTCCACGGATGTAGCCGCCAGCCAGCATGTGCTG GTTTGTGCTTTACAAGAACTTGGAAATCTGATCCACAACCTTGGCACTACAGCTGCGCCTTTGCTCCAGG ATTCCAGCACAGGCCTTCTGGACAGCGTCATCTCGGTGGTTCTGCACCCTAGCATTTCTGTTCGACTGGC TGCAGCCTGGTGCTTGCACTGCATTGCGGTGGCCTTGCCGTCCTACCTGACACCTCTCTTAGATCGTTGC CTAGAACGGCTCGCAATACTCAAGTCTTCACCAGAAGCAGTGACTGGCTTTAGTTCTGCTGTGGCGGCCT TGCTCGGTTCAGTGACACACTGTCCTTTGGGAATTCCTCATGGAAAGGGCAAGATTATCATGACAATAGC AGAGGATTTGCTGTGTTCCGCTGCTCAAAACAGCCGCCTGTCACTTCAGCGCACACAAGCGGGATGGCTG TTGGTTGCTGCTCTCATGACGCTAGGTCCTGCCGTGGTCAGTCACCACCTCACTCGAGTTCTGCTGCTGT GGAAGTGTGTCTTTCCAGCGTCCCCCAGAGATCTAGAAACAGAAAAGAGCCGAGGAGACTCGTTTACCTG GCAAGTGACCCTGGAAGGCCGAGCCGGTGCTCTCTGTGCTGTCAAGAGTTTCATTTCGCACTGTGGTGAT CTCCTTACTGAGGAAGTCATCCAGCGACTCCTGCCGCCGCTTCCCTGTGCTGTGGATCTGCTGACTCAGC TTTCTTCAATACTAAAAACATATGGAAGTTCTTTGAAAACACCGTCAATAGTTTATAGACAGAGACTTTA TGAACTACTAATTTTATTACCTCCTGAGACCTATAAAGGGAACCTCTGTGTTATCCTCAAAGAGCTGGCT GCCGAGCTGACGGCCCCTGATACCCAAGCAGCAGCATCGACGTGCTTACTGCCAGCCCTTTGCCATCCGG ATGACCTCTTGATATTAAGTCCTTTGCTGCAGGAGACCGACCATAGGTTTATTGAAGAGCAGCTCCTGCT TGGCAATGGTGTTGCCTGTGGAAGTCTAGAATATGACCCTTATTCTATTTATGAGAAGGACGTAGAAGGA GACTCTGTGCCTAAGCCCCTGCCTCCAGCACTGTCTGTGATTAGCTCTGCATCCAAGCTCTTTGGGGTTG TCTGTGCTACTGTGGATGAAGCTCAGAGGGTTCTGATACTGGAGCAGCTTTTGAACAGCATAAAGCACAC CAAAGGAGCTCGTCAGCAAACCGTTCAGTTGCATGTTGTTTCTGCCATTTCCAACTTACTGAAGTATGTG GCTGGCTCTAAGCAAAGCTTAGGTCCAGAGGTGAGAAGACTCGTCCTAACCCTAGTGTTGGGAGCACTCG AAAGCCCCACCCCCCTGCTGAGATGTGCAGCTTCGGAAGCATGGGCTAGGTTAGCCCAAGTGGCTGATGA TGGAGCCTTTACTGCTGGGTTAGCTCAACTTAGCTTCGACAAATTGAAGTCAGCGAGAGATGTGGTTACC AGAACAGGCCACTCACTGGCTCTGGGATCCCTGCATAGGTACTTAGGGGGCATAGGGCCTCAGCACTTGA GTTCTTGCATTGGAGTCCTTTATACCCTGTCTCAGGACAGCACTTCTCCTGACGTGCAGACCTGGGCACT CCACTCCTTGTCACTGACCATTGATTCTGCGGGTGCACTCTATCACGTGCATGTGGAGTCTACCCTGTCA CTCATCGTCATGTTGTTGCTAAATGTGCCTCCAACTCATGCGCAAGTTCACCAGAGTCTTGGTCGCTGCT TGAACGCCCTCATTACCACGTTAGGCCCAGAGCTACAAGGTAGCAATACTTCCGTGTCCGCCTTAAGGAC GTCCTGTCTGCTGGGCTGTGCAGTGATGCAGGATCACCCAGGCTGCCTGGTCCAGGCTCAGGCCATCTCT TGCCTTCAGCAGCTTCACATGTTTGCCCCACGCCATGTCAACTTGTCTAGTCTGGTCAGCTGCCTCTGTG TGAATCTTTGTAGCCCCTACCTGTTGCTGCGAAGAGCAGTTCTGGCTTGTTTGCGGCAGCTGGTACAGAG AGAAGCAGCTGAAGTTTCAGAGCATGCCATCATGCTGGCCAGGGATGGCAGGGACGCCGCTGCAGATGCT AACCTCAGAGAAGTGGGCCTTGAAGGGGCGCTGTTAGCCTTACTGGATAGAGAGACCGACGAAAGCTTGT GCCAGGATATCAGAGAGACATTGCATCATATGCTGACGTCCATGGCAGTAGGGAAGCTCACCCTTTGGCT GAAGCTTTGTAAAGATGTGCTTGCCGCATCCGCTGACTTTACAGCTGTAACTTGCGTGGATACAATGCAG GAGGAAGAAGGCGACAGAGGTGACGATGCCTCGGTTTTGACCAGAGGGGATGACAAGCCACACCCTTTCA GTAATCCTCGATGGGCCACTAGAGTCTTTGCTGCTGACTGTGTCTGCAGAATAATTAACCAGTGTGAAAA TGCAAACAGAGCTCATTTTGACATTGCTTTAGCACAAGAAATGAAAAAACGTGATTCAAGAAATGATTTT TTGGTGCTCCATCTTGCTGACTTGATCCGCATGGCTTTCATGGCTGCCACAGACCACAGTGACCAGCTCC GTCTCTCTGGCCTTGACACACTCTTGGTAGTTATCCGACGGTTTGCAGATATTGCAGAGCCAGAGTTTCC GGGTCATGTGATTCTGGAACAGTATCAAGCCAATGTTGGAGCCGCTCTTAGACCAGCCTTCACTTCAGAG ACACCACCTGACATCACCGCCAAAGCATGTCAGGTTTGCAGTGCTTGGATAGCAAGTGGGGTTGTTAGTG ACCTCAGCGATCTCCGCAGAGTTCATCAGCTACTTGTCTCTTCCCTGACGAAGATTCAGGCTGGAAAAGA AGCTCTCAGCCAGCTGTACAATGAGAGTGCCTCCACCATGGAGATCTTAGCTGTGCTGAGAGCCTGGGCA GAGGTCTATATAATTGCTGTACAAAGACATAAAAATCACAAGCAAGCCTTGAAGACTACTGTTAATTCTG AAGACAGTATGAGAAACGGGTCCTCTTCAGCTGCTGGTCTTCTTGACTTAGTCTGCACTGACCTGGCCAC GCTAAGCAAACTCTGGCTTGCTGCACTTCAGGATTTTGCTCTCTTAACTTTGCCTGCAGAATTTGCTTCC CAGCTTCCTACTGAAGGTGGTGCTTTCTACACAGCAGAGACGAGCAAGAGTGCAAAGCTACACTACCACG ACTCCTGGGCCCTCATCCTCCACGCTGCGGCCCTGTGGCTCACCAGCACGGGCTTCGCTGACCCAGATGA AGGCGGTGCCAATCTCTCAAGGCCTGTAACTCCAACATCCATGTGCCAGGGCTCATCATCATCAGGAGCT GCCGTGAAGTCCCCCGAGGATGTCTACACTGACAGGTTCCATCTGATTCTAGGAATCAGCGTGGAGTTCC TGTGTTCCCTGCGCTCAGATGCAAGCTTGGAAAGCATCATGGCTTGTCTGCGTGCACTGCAGGCCCTGCT CGATGTTCCTTGGCCCAGGTGGAGAATTGGCAGTGATCAGGACTTGGGTATTGAATTGCTAAATGTACTA CACCGAGTAATTTTGACCAGAGAGTCACCAGCCATTCAACTGGCTTCACTTGAAGTGGTCAGGCAGATTA TCTGTGCCGCCCAAGAACATGTGAAGGAAAAAAGACGTAGTGCAGAAGTTGATGATGGAGCCTCTGAGAA GGAAACCCTGCCAGAGTTTGGTGAAGGGAAGGACACAGGAGGACTCGTACCTGGGAAGTCTTTGGTCTTT GCAACCCTGGAACTCTGTGTCTGCATCCTCGTTAGACAGCTCCCAGAACTGAACCCTAAGCTGGCAGGTA GCCCAGGAGGAAAGGCTTCAAAGCCGAAGACCCTGTTGGAGGAGGGAAGTAGACTGGTGGCGGCTGCCCT GGCCATCCTTGCTGAGCTTCCTGCAGTGTGCTCTCCTGAAGGCAGCATCTCAATTCTCCCTACAGTATTG TACCTTACCATCGGAGTCCTCCGGGAAACGGCTGTGAAGTTACCTGGGGGCCAGTTATCCTGCACAGTCA CGGCTTCCCTGCAGACTCTGAAAGGAATCTTAACTTCCCCCATGGCCCGGGCAGAAAAGAGCCACGAAGC TTGGACCAGCCTCCTCCAAAGTGCATTAGCAACTGTGCTTGACTGCTGGAGCCCAGTTGACGGAGCACAA GAACCTGATGAAGTCAGTCTGCTTACTGCCGTCACAGTATTTATTTTGTCTACCAGCCCAGAAGTGACAA CCGTCCCCTGCCTTCAGAATCGCTGCATTGAAAAATTTAAGGCTGCCCTGGAGAGCAAGGACTCTGTGGT GCAAATGAAGACCTGTCAGCTCCTCCACTCCATTTTTCAGTATCCAAAGCCGGCCGTTTCCTACCCATAC ATTTATTCCTTAGCATCTTCTATCGTGGAGAAGCTTCAGGACATAGCCAGGAGGAAACCCGAAGATGCTA CCGAGCTGCAGCTCTGTCAAGAAGGAATAAAGCTCTTAGAAGCTTTGGTCGCCATTGCGGAAGAAGAGCA CCGCGCTCAGCTGGTGGCCTGCCTTCTGCCCATCCTCATCTCCTTCCTTTTGGATGAGAATGCTCTGGGA TCAGCAACTTCAGTAACGAGAAGTCTGCACGACTTTGCTTTGCACAGTCTCATGCAGATTGGGCCTCGGT ACTCGTCTGTGTTTAAAAGAGTCATGGCTTCTTCCCCAGCCCTGAAAGCCCGGCTGGAGGCTGCTGTAAA AGGCAATCAGGAAAGTGTCCGAGTGGATCCGCCTTCTAAGCATGCCAAGAACCTGGCCAGGAACTCCAGC ATCCAGCTAAAGACCAATTTCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR212107 representing NM_177171
Red=Cloning site Green=Tags(s) MELAHSLLLNEEASNQLGAVQKAEFIFEWLRYLEKLLLATNREDVREKQKTLVGQLLSLLNSSPGPPTRK LLAQDLAILYSVGDTVSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILANGFTDTVVN ILKAMKSAESQGRYEIMLSLQSILTGLGAAAAPCHRDVYKAARSCLTDRSMAVRCAAAKCLLELQNEAIF MWSTDVDSVATLCFKSFEGSNYDVRISVSKLLGTVLAKAVTAKHPGAGSKQSARRVSLEEVLELLGAGFL RGSSGFLRASGDMLKGNSSVSRDVRVGVTQAYVVFVSTLGGAWLEKNLAAFLSHILSLVSQSNPKATQTQ IDAVCCRRCVSFILRATLGGLLGEKAQIAAAKEICQAVWRLKKVMDAALSDGNVETRLSSTDVAASQHVL VCALQELGNLIHNLGTTAAPLLQDSSTGLLDSVISVVLHPSISVRLAAAWCLHCIAVALPSYLTPLLDRC LERLAILKSSPEAVTGFSSAVAALLGSVTHCPLGIPHGKGKIIMTIAEDLLCSAAQNSRLSLQRTQAGWL LVAALMTLGPAVVSHHLTRVLLLWKCVFPASPRDLETEKSRGDSFTWQVTLEGRAGALCAVKSFISHCGD LLTEEVIQRLLPPLPCAVDLLTQLSSILKTYGSSLKTPSIVYRQRLYELLILLPPETYKGNLCVILKELA AELTAPDTQAAASTCLLPALCHPDDLLILSPLLQETDHRFIEEQLLLGNGVACGSLEYDPYSIYEKDVEG DSVPKPLPPALSVISSASKLFGVVCATVDEAQRVLILEQLLNSIKHTKGARQQTVQLHVVSAISNLLKYV AGSKQSLGPEVRRLVLTLVLGALESPTPLLRCAASEAWARLAQVADDGAFTAGLAQLSFDKLKSARDVVT RTGHSLALGSLHRYLGGIGPQHLSSCIGVLYTLSQDSTSPDVQTWALHSLSLTIDSAGALYHVHVESTLS LIVMLLLNVPPTHAQVHQSLGRCLNALITTLGPELQGSNTSVSALRTSCLLGCAVMQDHPGCLVQAQAIS CLQQLHMFAPRHVNLSSLVSCLCVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAIMLARDGRDAAADA NLREVGLEGALLALLDRETDESLCQDIRETLHHMLTSMAVGKLTLWLKLCKDVLAASADFTAVTCVDTMQ EEEGDRGDDASVLTRGDDKPHPFSNPRWATRVFAADCVCRIINQCENANRAHFDIALAQEMKKRDSRNDF LVLHLADLIRMAFMAATDHSDQLRLSGLDTLLVVIRRFADIAEPEFPGHVILEQYQANVGAALRPAFTSE TPPDITAKACQVCSAWIASGVVSDLSDLRRVHQLLVSSLTKIQAGKEALSQLYNESASTMEILAVLRAWA EVYIIAVQRHKNHKQALKTTVNSEDSMRNGSSSAAGLLDLVCTDLATLSKLWLAALQDFALLTLPAEFAS QLPTEGGAFYTAETSKSAKLHYHDSWALILHAAALWLTSTGFADPDEGGANLSRPVTPTSMCQGSSSSGA AVKSPEDVYTDRFHLILGISVEFLCSLRSDASLESIMACLRALQALLDVPWPRWRIGSDQDLGIELLNVL HRVILTRESPAIQLASLEVVRQIICAAQEHVKEKRRSAEVDDGASEKETLPEFGEGKDTGGLVPGKSLVF ATLELCVCILVRQLPELNPKLAGSPGGKASKPKTLLEEGSRLVAAALAILAELPAVCSPEGSISILPTVL YLTIGVLRETAVKLPGGQLSCTVTASLQTLKGILTSPMARAEKSHEAWTSLLQSALATVLDCWSPVDGAQ EPDEVSLLTAVTVFILSTSPEVTTVPCLQNRCIEKFKAALESKDSVVQMKTCQLLHSIFQYPKPAVSYPY IYSLASSIVEKLQDIARRKPEDATELQLCQEGIKLLEALVAIAEEEHRAQLVACLLPILISFLLDENALG SATSVTRSLHDFALHSLMQIGPRYSSVFKRVMASSPALKARLEAAVKGNQESVRVDPPSKHAKNLARNSS IQLKTNFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177171 |
| ORF Size | 6114 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177171.4, NP_796145.2 |
| RefSeq Size | 7761 bp |
| RefSeq ORF | 6117 bp |
| Locus ID | 320487 |
| UniProt ID | Q5PRF0 |
| Cytogenetics | 12 B3-C1 |
| MW | 219.9 kDa |
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