Nup188 (NM_198304) Mouse Tagged ORF Clone

CAT#: MR212080

Nup188 (Myc-DDK-tagged) - Mouse nucleoporin 188 (Nup188)

Product Images

Circular map for MR212080

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nup188
• Synonyms: BC025526; mKIAA0169; U89435
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR212080 representing NM_198304
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTGCCGCTGGTGGGCCGTGTGTGAGGAGCAGTAGAGAACTCTGGACAATTCTACTTGGGAGGT
  CAGCTCTTAGAGAATTGAATCAGATTGAGGCGGAACTGAATAAATATTGGCAGCGGTTGTTAGAAGGGCT
  TTCTTACTACAAACCTCCCAGTCCAAGTTCAGCTGAAAGAGTGAAAGCTAATAAAGACGTAGCGTCACCT
  CTGAAGGAGCTGGGCCTAAGAGTCAGCAAGTTCTTGGGTCTTGATGAAGAGCAGAGTGTGCAGTTACTGC
  AGTGTTACCTTCAGGAGGATTACAGGGGTACTCGGGACTCACTAAAGACAGTCCTGCAAGATGAGAGGCA
  GAGCCAGGCCTTAACCCTGAAGATTGCAGATTATTATTATGAAGAAAGAACCTGCATTCTTCGTTGTGTC
  TTACACCTTCTGACATATTTCCAAGATGAACGCCATCCATACAGGGCGGAATATGCTGACTGTGTTGACA
  AACTGGAGAAGGAACTAGTTTTAAAGTACCGGCAGCAGTTTGAGGAGCTGTATAGAACAGAAGCACCAAC
  CTGGGAGACGCATGGCAACCTCATGACTGAGCGTCAGGTGTCGCGGTGGTTGGTTCAGTGCCTCCGGGAA
  CAGTCCATGCTGTTAGAAATTATTTTCCTTTATTATGCATACTTCGAGATGGCACCAAGTGACCTATTGG
  TACTAACCAAGATGTTCAAGGAGCAAGGGTTTGGTAGTAGGCAGACCAGCAGGCACCTTGTGGGTGGAAC
  CATGGATCCTTTTGTAGATCGCATTGGGTACTTCAGTGCCCTCATCCTGGTGGAAGGGATGGACATCGAG
  TCCCTGCACAAGTACGCCTTGGATGACAGGCGAGAGCTGCACCAGTTTGCTCAGGATGGCCTCATCTGCC
  AGGACATGGACCGAGCGATGTTGACCTTGGGAGACATTCCGCACCACGCCCCTGTGCTTTTAGCCTGGGC
  TCTCCTCCGTCACACACTGAGCCCAGAAGAGACCAGCAGTGTGGTCAGGAAGATAGGGGGCACGGCCATC
  CAGCTGAATGTGTTTCAGTACTTGACTCGACTGCTTCGGTCACTTGCCAGTGGAGGAAATGATTGCACCA
  CGAGCACCGCCTGCATGTGTGTCTATGGTCTTCTCTCCTTTGCCCTCACCTCACTGGAGCTGCACACACT
  GGGCAACCAGCAGGATGTCATTGACACAGCATGTGAAGTCCTGGCAGACCCTTCCCTTCCAGAGCTGTTC
  TGGGGAACAGAGCCAACCTCTGGTCTCGGGATCATTCTGGACAGTGTGTGTGGGATGTTTCCCCATCTCC
  TTTCCCCACTTCTGCAATTGCTCCGAGCCCTCGTGTCAGGGAAGTCCACTGCCAAAAAAGTGTACAGCTT
  CCTGGATAAAATGTCTTTCTACAACGAACTCCATAAGCACAAGCCCCATGATGTGCTCTCTCATGAAGAT
  GGAACTCTGTGGCGGAGGCAAACACCCAAACTCCTGTACCCTCTAGGGGGTCAGACCAACCTTCGCATTC
  CTCAGGGCACTGTGGGCCAAGTCATGCTGGATGACAGGGCTTACCTTGTCCGCTGGGAGTACTCTTACAG
  CAGCTGGACCCTCTTCACCTGCGAGATTGAAATGCTGCTCCACGTTGTCTCCACAGCAGATGTGATCCAG
  CATTGCCAGCGGGTCAAGCCCATCATTGATTTGGTTCATAAGGTCATCAGCACAGACTTGTCCATAGCAG
  ACTGCCTGCTGCCCATCACATCCCGCATCTACATGCTGTTGCAGCGGCTGACGACGGTGATCTCCCCTCC
  TGTAAACGTCATTGCTTCTTGTGTCAACTGCTTAACTGTCTTGGCTGCTCGAAACCCAGCAAAGGTCTGG
  ACTGATCTTCGTCACACAGGTTTTTTGCCATTCGTGGCCCACCCTGTCTCCAACATGACTCAGATGATTA
  GTGCGGAGGGAATGAATGCTGGAGGGTATGGCAGCCTTCTGATGAACAGTGAGCAGCCCCAGGGCGAATA
  TGGGGTCACCATTGCCTTTCTGCGCCTGGTCACCACGCTTGTCAAGGGGCAGCTTGGTAGTACCCAGAGC
  CAAGGCCTGGTGCCTTGTGTGATGTTCGTGTTGAAGGAGATGCTTCCCAGCTACCACAAATGGCGCTACA
  ACTCTCATGGCGTGCGAGAGCTGATAGGTTGCCTAATTCTGGAGCTGATTCATGCAATACTGAACCTGTG
  CCAAGAGACAGAGCTGCATAGCAGCCACACACCCAGCCTGCCTTCCCTCTGCATCTGCAGCCTGGCTTAT
  ACCGAAGCTGGACAGACCGTCATTAGCATCATGGGAATTGGTGTAGACACCATAGACATGGTGATGGCTG
  CTCAGCCCCGCAGTGATGGGCCAGAAGGCCAAGGCCAGGGCCAACTGCTGATCAAGACAGTGAAGCTGGC
  GTTCTCTGTCACCAACAATGTTATTCGGCTGAAACCTCCTTCTAATGTGGTTTCACCCCTGGAACAGGCT
  CTCACACAACATGGTGCCCACGGGAACAACCTCATTGCTGTCCTAGCCAAGTACATCTACCACAGACATG
  ACCCAGCCTTGCCACGTCTGGCCATCCAGCTGCTGAAACGCTTGGCCACGGTGGCCCCAATGTCAGTGTA
  TGCTTGCCTGGGCAGTGATGCAGCGGCCATCCGTGATGCCTTCCTGACCCGCCTTCAGAGCAAGATTGAG
  GACATGCGCATCAAAGTCATGATCCTGGAGTTCCTGACAGTGGCGGTAGAGACACAGCCAGGTCTCATCG
  AGCTGTTTCTGAATCTGGAGGTGAAGGATGGCAGCAATGGCTCCAAGGAATTCAGCCTTGGTGTGTGGAG
  CTGTCTCCATGTGGTGCTGGAGCTGATTGATTCCCAGCAGCAAGATCGATACTGGTGCCCACCTCTGTTG
  CATCGTGCAGCTATTGCCTTCCTTCATGCCCTGTGGCAAGACCGTAGGGACAGTGCAATGCTTGTCCTCA
  GAACCAAACCCAAGTTTTGGGAGAATTTAACAAGTCCGCTGTTTGGAACTCTCTCTCCTCCTTCAGAGAC
  ATCTGAGCCCAGTGTCCTGGAAACCTGTGCCCTGATCATGAAGATAATATGTTTGGAGATATACTATGTA
  GTTAAGGGCTCGTTAGACCAGTCATTAAAAGATACACTCAAGAAGTTCTCCAGTGAGAAACGCTTTGCCT
  ACTGGTCAGGGTACGTCAAGTCTTTGGCAGTTTACATGGCTGACACAGAGGGCAGCAGTTGCACGTCCCT
  GCTAGAGTATCAGATGCTGGTCTCCGCCTGGAGGATACTTCTCATCATTGCTGCCAGTCACGCAGACGTC
  ATGCACTTGACTGACATGGCAGTGCGGCGCCAGCTGTTTCTTGATGTGCTTGATGGGACCAAAGCATTAC
  TCCTTGTGGCAGCATCTGTGAACTGCCTCCGGCTGGGCTCCATGATGTGCACCCTGCTGCTTATCCTCCT
  ACGCCAGTGGAAGAGAGAGCTGGGTGCTGTGGAGAAAATCCTTGGGCCCTTGACAGAGATCCTGGAAGGG
  GTGCTTCAGGCAGACCAGCAGCTCATGGAAAAGACCAAGGCCAAGGTGTTCTCTGCATTCATCACAGTGT
  TACAGATGAAGGAGCTGCGAGTCGGTGATATCCCCCAGTACTCCCAGCTGGTGCTGAACGTCTGTGAGAC
  TCTCCAAGAAGAGGTGATTGCACTCTTTGATCAGACCCGCCACAGCCTGGCATCCGACAGCGCTGCCGAG
  GACAAGGACAGCATGGAGACAGATGACTGCCCTCGGCCCCGGCACAAGGACCAGCGTGATGGGGTGTGTG
  TCCTGGGACTGCATCTGGCTAAGGAGCTCTGTGAGGTGGATGAGGATGGGGACTCATGGTTGCAGGTGAC
  CCGAAGGCTCCCCATTCTGCCCACGCTGCTCACCACGCTGGAGGTGAGCCTCCGCATGAAGCAGAACCTG
  CATTTTACTGAGGCTGCGCTGCACCTGCTCCTCACCCTGGCTCGAACCCAGCAAGGAGCCACAGCAGTGG
  CTGGAGCTGGCATCACCCAGAGCATCTGTTTGCCCCTCTTGAGTGTATACCAGCTTAGCAGCAATGGCAC
  AGGACAGACACCTAGCACCTCTCGAAAGTCCCTGGATGCCCCATCCTGGCCAGGAGTCTACCGCCTGTCC
  ATGTCCTTAATGGAGCGATTGCTCAAGACTCTACGCTACAATTTCCTGACGGAGGCTCTAGACTTTGTGG
  GTGTCCATCAAGAACGGACCTTGCAGTGTCTCAATGCCGTGAAGACAGTGCAGAGTCTAGCCTGCCTGGA
  GGAAGCAGATCACACCGTGGGCTTCATCCTACAACTGTCCCACTTCAGGAAGGAGTGGCACTTCCATCTG
  CCTCAGCTCATGCGTGATGTTCAGGTGAACCTGGGTTACTTGTGCCAGGCCTGTACCTCCCTCCTGCACA
  GCCGCAAGATGCTGCAGCACTACCTACAGAACAAAAATGGTGATGGCCTCCCTTCAGCTGTCACTCCCCG
  AGCTCAGCGGCCATCCACCACCACCACCACCACCACCACCACCACCGCTCTGGCTACCCCAGCTGGCTGC
  TCCTCCAAGCAGCCTACTGCTGACACTGAGGCCTCAGAACAGCGAGCCTTACACACAGTCCAGTATGGGC
  TTCTCAAGATTCTCAGCAGGACCCTGGCAGCCCTGCGCCACTTCACTCCGGATGTCTGCCAGATCCTGTT
  GGACCAGTCCCTGGATCTTGCTGAGTACAACTTCCTCTTTGCCCTAAGTTTTACCACTCCCACCTTTGAC
  TCTGAAGTGGCTCCCTCCTTCGGAACCCTCTTGGCCACAGTGAACGTGGCCCTCAACATGCTTGGAGAGC
  TGGACAAGAAAAAGGAATCCCTCACCCAGGCTGTGGGACTCAGCACACAAGCAGAAGGGACCCGAACATT
  AAAGTCCCTCCTGATGTTCACCATGGAGAACTGCTTCTACCTGCTCATCTCCCAAGCAGTACGCTACCTC
  AGGGACCCGGCTGTGCACCCCCGGGACAAGCAGCGGATGAAGCAGGAGCTGAGCTCGGAGCTGAGCACGT
  TGCTTTCCAGCCTCTCACGATACTTCAGAAGGGGGGCCCCCAGCTCCCCTGCTGCTGGAGTCCTGCCCTC
  ACCACAGGGCAAGGCCACCTCTCTCTCCAAAGCCAGCCCAGAATCTCAGGAGCCTCTGATCCAACTTGTG
  CAAGCCTTTGTCCGGCACGTGCAGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR212080 representing NM_198304
  Red=Cloning site Green=Tags(s)
  
  MAAAAGGPCVRSSRELWTILLGRSALRELNQIEAELNKYWQRLLEGLSYYKPPSPSSAERVKANKDVASP
  LKELGLRVSKFLGLDEEQSVQLLQCYLQEDYRGTRDSLKTVLQDERQSQALTLKIADYYYEERTCILRCV
  LHLLTYFQDERHPYRAEYADCVDKLEKELVLKYRQQFEELYRTEAPTWETHGNLMTERQVSRWLVQCLRE
  QSMLLEIIFLYYAYFEMAPSDLLVLTKMFKEQGFGSRQTSRHLVGGTMDPFVDRIGYFSALILVEGMDIE
  SLHKYALDDRRELHQFAQDGLICQDMDRAMLTLGDIPHHAPVLLAWALLRHTLSPEETSSVVRKIGGTAI
  QLNVFQYLTRLLRSLASGGNDCTTSTACMCVYGLLSFALTSLELHTLGNQQDVIDTACEVLADPSLPELF
  WGTEPTSGLGIILDSVCGMFPHLLSPLLQLLRALVSGKSTAKKVYSFLDKMSFYNELHKHKPHDVLSHED
  GTLWRRQTPKLLYPLGGQTNLRIPQGTVGQVMLDDRAYLVRWEYSYSSWTLFTCEIEMLLHVVSTADVIQ
  HCQRVKPIIDLVHKVISTDLSIADCLLPITSRIYMLLQRLTTVISPPVNVIASCVNCLTVLAARNPAKVW
  TDLRHTGFLPFVAHPVSNMTQMISAEGMNAGGYGSLLMNSEQPQGEYGVTIAFLRLVTTLVKGQLGSTQS
  QGLVPCVMFVLKEMLPSYHKWRYNSHGVRELIGCLILELIHAILNLCQETELHSSHTPSLPSLCICSLAY
  TEAGQTVISIMGIGVDTIDMVMAAQPRSDGPEGQGQGQLLIKTVKLAFSVTNNVIRLKPPSNVVSPLEQA
  LTQHGAHGNNLIAVLAKYIYHRHDPALPRLAIQLLKRLATVAPMSVYACLGSDAAAIRDAFLTRLQSKIE
  DMRIKVMILEFLTVAVETQPGLIELFLNLEVKDGSNGSKEFSLGVWSCLHVVLELIDSQQQDRYWCPPLL
  HRAAIAFLHALWQDRRDSAMLVLRTKPKFWENLTSPLFGTLSPPSETSEPSVLETCALIMKIICLEIYYV
  VKGSLDQSLKDTLKKFSSEKRFAYWSGYVKSLAVYMADTEGSSCTSLLEYQMLVSAWRILLIIAASHADV
  MHLTDMAVRRQLFLDVLDGTKALLLVAASVNCLRLGSMMCTLLLILLRQWKRELGAVEKILGPLTEILEG
  VLQADQQLMEKTKAKVFSAFITVLQMKELRVGDIPQYSQLVLNVCETLQEEVIALFDQTRHSLASDSAAE
  DKDSMETDDCPRPRHKDQRDGVCVLGLHLAKELCEVDEDGDSWLQVTRRLPILPTLLTTLEVSLRMKQNL
  HFTEAALHLLLTLARTQQGATAVAGAGITQSICLPLLSVYQLSSNGTGQTPSTSRKSLDAPSWPGVYRLS
  MSLMERLLKTLRYNFLTEALDFVGVHQERTLQCLNAVKTVQSLACLEEADHTVGFILQLSHFRKEWHFHL
  PQLMRDVQVNLGYLCQACTSLLHSRKMLQHYLQNKNGDGLPSAVTPRAQRPSTTTTTTTTTTALATPAGC
  SSKQPTADTEASEQRALHTVQYGLLKILSRTLAALRHFTPDVCQILLDQSLDLAEYNFLFALSFTTPTFD
  SEVAPSFGTLLATVNVALNMLGELDKKKESLTQAVGLSTQAEGTRTLKSLLMFTMENCFYLLISQAVRYL
  RDPAVHPRDKQRMKQELSSELSTLLSSLSRYFRRGAPSSPAAGVLPSPQGKATSLSKASPESQEPLIQLV
  QAFVRHVQR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198304
• ORF Size: 5277 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198304.3
• RefSeq Size: 5670 bp
• RefSeq ORF: 5280 bp
• Locus ID: 227699
• UniProt ID: Q6ZQH8
• Cytogenetics: 2 B
• MW: 196.7 kDa
• Gene Summary: May function as a component of the nuclear pore complex (NPC).[UniProtKB/Swiss-Prot Function]