Eif4g1 (NM_145941) Mouse Tagged ORF Clone

CAT#: MR212048

Eif4g1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 1

Product Images

Circular map for MR212048

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eif4g1
• Synonyms: E030015G23Rik; eIF-4-gamma 1; eIF-4G 1; eIF-4G1; eIF4GI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR212048 representing NM_145941
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAAAGCTCCCCAGCCCACAGGCCCCCCACCCGCCAGGTCCCCTGGACTCCCACAGCCAGCGTTTC
  CCCCGGGGCAGACTGCACCGGTGGTGTTTAGCACGCCTCAAGCGACACAAATGAACACGCCTTCTCAGCC
  CCGCCAGGGAGGATTCAGGTCTCTGCAGCACTTCTACCCTAGCCGGGCCCAGCCCCCTAGCAGTGCAGCC
  TCCCGAGTGCAGAGTGCAGCCCCTGCCCGTCCTGGCCCAGCTCCCCATGTCTACCCTGCTGGATCCCAAG
  TAATGATGATCCCTTCCCAGATCTCCTACTCAGCCTCCCAAGGAGCCTACTATATCCCTGGACAGGGGCG
  TTCCACATATGTTGTCCCAACACAGCAGTACCCTGTGCAGCCAGGAGCCCCAGGCTTCTATCCAGGTGCA
  AGCCCTACCGAGTTTGGGACCTATGCTGGAGCCTATTACCCAGCCCAAGGTGTGCAGCAGTTTCCTGCTA
  GTGTGGCTCCTGCCCCAGTTTTGATGAACCAGCCACCCCAGATTGCTCCTAAGAGGGAACGGAAAACTAT
  CCGAATTCGAGACCCAAACCAAGGAGGGAAGGATATCACAGAAGAGATCATGTCTGGGGCCCGCACTGCC
  TCCACACCCACTCCTCCCCAGACGGGAGGCAGTCTGGAGCCTCAACCCAATGGGGAGTCGCCTCAGGTTG
  CTGTCATTATCCGGCCAGATGACCGGTCGCAGGGAGCAGCCATTGGGGGGCGGCCAGGACTGCCTGGCCC
  AGAGCATAGCCCTGGCACAGAATCTCAGCCTTCGTCGCCTTCTCCAACCCCATCACCACCCCCAATTTTG
  GAGCCGGGGTCTGAGTCTAATCTTGGAGTCCTCTCTATTCCTGGGGACACTATGACAACAGGGATGATAC
  CAATGTCTGTAGAAGAATCGACCCCTATCTCTTGTGAAACTGGGGAGCCGTATTGCCTCTCTCCAGAACC
  CACTCTTGCCGAACCCATACTGGAAGTAGAAGTGACACTCAGCAAACCCATTCCAGAATCTGAGTTCTCT
  TCCAGTCCTCTCCAGGTTTCCACGGCCCTGGTGCCTCACAAGGTTGAAACTCATGAGCCCAATGGCGTGA
  TCCCATCTGAGGATCTGGAACCAGAGGTGGAGTCAAGCACAGAGCCAGCTCCTCCCCCTCTATCACCTTG
  TGCTTCTGAATCGCTCGTGCCCATTGCTCCAACTGCCCAGCCTGAGGAACTGCTCAACGGAGCCCCCTCA
  CCACCAGCTGTGGATTTAAGCCCAGTCAGTGAGCCAGAGGAACAGGCCAAGAAGGTTTCATCAGCAGCAC
  TGGCCAGCATTCTCTCTCCTGCTCCACCTGTGGCTCCTTCAGATACTTCTCCTGCTCAGGAGGAAGAAAT
  GGAAGAAGATGACGATGACGAAGAAGGTGGAGAAGCTGAGAGTGAGAAGGGAGGAGAGGACGTCCCCCTT
  GACAGTACTCCTGTCCCAGCCCAGCTGTCTCAGAATTTAGAGGTGGCAGCAGCTACCCAGGTGGCAGTGT
  CTGTGCCAAAGAGGAGACGGAAAATTAAAGAGCTAAATAAGAAGGAGGCTGTAGGAGACCTTCTAGATGC
  CTTCAAGGAGGTGGACCCAGCAGTACCAGAGGTAGAGAATCAGCCTCCCACTGGCAGCAACCCAAGCCCA
  GAGTCTGAGGGCAGCATGGTGCCCACACAGCCTGAGGAAACAGAAGAAACCTGGGACTCTAAGGAAGACA
  AAATTCATAATGCTGAGAACATCCAGCCTGGGGAGCAGAAGTATGAGTACAAGTCAGATCAGTGGAAGCC
  TCTAAACCTTGAAGAAAAGAAGCGTTATGACAGAGAATTCCTGCTGGGCTTTCAGTTCATCTTTGCCAGC
  ATGCAGAAGCCAGAAGGATTGCCCCATATCACTGATGTGGTGCTGGACAAGGCCAATAAAACACCACTTC
  GGCAACTGGATCCCTCCAGACTACCTGGCATAAACTGTGGCCCAGATTTCACTCCATCTTTTGCCAATCT
  TGGCCGACCAGCCCTCAGCAACCGTGGGCCCCCGAGGGGTGGGCCAGGTGGGGAGCTGCCCCGAGGGCCG
  GCTGGCCTGGGACCCAGGCGCTCTCAGCAGGGCCCACGAAAGGAAACTCGCAAGATCATTTCCTCAGTGA
  TAATGACTGAAGACATAAAACTAAATAAAGCAGAGAAGGCTTGGAAACCCAGTAGCAAACGGACAGCAGC
  TGATAAGGATCGAGGGGAAGAGGATGCTGATGGAAGCAAGACCCAGGACCTGTTCCGCAGGGTACGCTCC
  ATCCTGAATAAGCTGACACCCCAGATGTTTCAGCAGCTGATGAAGCAGGTGACACAGCTGGCCATTGACA
  CGGAGGAACGCCTCAAAGGAGTTATTGACCTCATCTTTGAGAAAGCCATTTCAGAGCCCAACTTCTCTGT
  GGCTTATGCCAACATGTGCCGCTGCCTCATGGCGCTGAAAGTGCCCACTACAGAAAAGCCAACAGTAACT
  GTGAATTTTCGAAAACTGTTGTTAAATCGGTGCCAGAAGGAATTTGAGAAAGACAAAGATGATGATGAAG
  TTTTTGAAAAAAAGCAAAAAGAGATGGATGAAGCTGCTACGGCAGAAGAACGGGGACGCCTGAAAGAAGA
  GCTAGAAGAAGCCCGGGACATAGCTAGGCGGCGCTCTTTAGGGAATATCAAGTTTATTGGAGAGTTATTC
  AAGTTGAAGATGTTAACAGAAGCAATAATGCATGACTGTGTGGTCAAGCTACTTAAGAACCATGATGAGG
  AGTCCCTGGAATGCCTCTGCCGCCTCCTCACCACTATAGGCAAAGACCTGGACTTTGCAAAAGCCAAGCC
  TCGAATGGATCAGTATTTCAACCAAATGGAAAAAATCATTAAAGAAAAGAAGACCTCATCTCGCATCCGT
  TTTATGCTGCAGGATGTACTCGATCTGCGGCAGAGTAATTGGGTGCCACGCCGAGGGGATCAGGGTCCTA
  AGACTATTGATCAAATTCACAAGGAGGCTGAGATGGAAGAGCACCGAGAACATATTAAAGTACAGCAGCT
  CATGGCCAAGGGCAGCGACAAACGTCGGGGTGGCCCTCCAGGCCCGCCTATCAACCGTGGCCTTCCACTT
  GTAGATGATGGTGGCTGGAATACAGTCCCCATTAGCAAAGGCAGTCGCCCCATTGACACCTCACGGCTCA
  CCAAGATCACAAAGCCTGGTTCCATTGATTCTAACAACCAGCTTTTTGCACCTGGAGGACGACTGAGTTG
  GGGCAAGGGCAGCAGTGGGGGCTCAGGAGCCAAGCCCTCAGACACAGCATCGGAAGCTACTCGTCCAGCT
  ACCTTGAATCGCTTTTCTGCTCTTCAACAAACATTACCCGCAGAGAACACAGATAACAGACGTGTTGTAC
  AGAGGAGTAGCTTGAGCCGGGAACGAGGTGAGAAAGCTGGGGACCGGGGAGACCGACTAGAGCGGAGTGA
  GCGGGGAGGTGACCGTGGGGACCGACTTGATCGTGCCAGAACACCTGCCACCAAGCGAAGTTTTAGCAAA
  GAAGTGGAGGAGCGAAGTAGAGAGCGGCCATCCCAGCCTGAGGGACTCCGCAAGGCAGCTAGCCTCACAG
  AGGATCGTGGTCGGGATCCTGTGAAGCGGGAAGCCACTCTACCTCCAGTGAGCCCTCCAAAGGCTGCGCT
  GTCTGTGGATGAGGTGGAGAAGAAATCTAAGGCCATCATTGAGGAATATCTCCATCTCAATGACATGAAG
  GAGGCAGTACAGTGTGTCCAGGAGCTGGCTTCACCCTCCTTGCTCTTCATCTTCGTACGGCTTGGTATCG
  AGTCCACGCTGGAGCGTAGTACCATTGCTCGTGAGCATATGGGGCGACTACTGCACCAACTGCTCTGTGC
  GGGGCACCTCTCTACTGCCCAGTACTATCAAGGGCTGTATGAAACACTAGAATTGGCTGAGGACATGGAA
  ATTGATATCCCTCATGTATGGCTTTACCTGGCAGAACTGATTACACCTATTCTCCAGGAAGATGGGGTGC
  CCATGGGAGAGCTCTTCAGGGAAATTACGAAGCCTCTGAGACCCATGGGCAAAGCTACTTCTTTATTGCT
  GGAGATCCTGGGTCTCTTATGCAAGAGCATGGGTCCCAAAAAGGTGGGGATGCTGTGGCGAGAGGCTGGG
  CTGAGCTGGAGGGAATTTCTAGCAGAGGGCCAAGACGTTGGCTCATTTGTGGCTGAAAAGAAGGTGGAAT
  ATACCTTGGGAGAAGAATCTGAAGCTCCTGGCCAGAGGACACTTGCCTTTGAGGAGCTTCGTAGGCAGCT
  AGAGAAGCTGCTGAAGGACGGCGGCAGTAATCAGCGTGTGTTCGACTGGATAGACGCCAACCTAAATGAG
  CAGCAGATAGCATCCAATACATTAGTTCGAGCCCTCATGACAACTGTCTGTTATTCTGCAATTATCTTTG
  AGACTCCTCTCCGAGTGGATGTTCAAGTGTTGAAAGTGCGAGCAAGACTGCTGCAGAAATACCTGTGTGA
  TGAGCAGAAGGAGCTACAAGCACTCTATGCTCTCCAGGCCCTTGTAGTGACCTTAGAACAGCCTGCCAAC
  CTGCTTCGGATGTTCTTTGATGCTCTATATGATGAGGACGTGGTGAAGGAAGACGCCTTCTACAGCTGGG
  AGAGCAGCAAGGATCCTGCTGAACAGCAGGGCAAGGGTGTGGCCCTTAAATCTGTCACAGCATTCTTCAA
  TTGGCTTCGTGAGGCTGAGGACGAGGAGTCTGATCACAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR212048 representing NM_145941
  Red=Cloning site Green=Tags(s)
  
  MNKAPQPTGPPPARSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQGGFRSLQHFYPSRAQPPSSAA
  SRVQSAAPARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGA
  SPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTA
  STPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPIL
  EPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFS
  SSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPS
  PPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPL
  DSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSP
  ESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFAS
  MQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGP
  AGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRS
  ILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVT
  VNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELF
  KLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIR
  FMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPL
  VDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPA
  TLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSK
  EVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMK
  EAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDME
  IDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAG
  LSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNE
  QQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPAN
  LLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145941
• ORF Size: 4800 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145941.3, NP_666053.2
• RefSeq Size: 5481 bp
• RefSeq ORF: 4803 bp
• Locus ID: 208643
• UniProt ID: Q6NZJ6
• Cytogenetics: 16 B1
• MW: 176.5 kDa
• Gene Summary: This gene encodes a member of the eukaryotic translation initiation factors (eIF) that play important roles in translation initiation by mediating recruitment of additional initiation factors and providing a scaffold for ribosome/mRNA-bridging. Along with eIF4A and eIF4E, the encoded protein forms the eIF4F complex that bridges the 5' UTR with the polyadenylated 3' UTR resulting in mRNA circularization, enhanced translation initiation and mRNA stability. Through its association with eIF3, the encoded protein mediates recruitment of the 43S pre-initiation complex to mRNA. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes for this gene have been identified on chromosomes 2 and 13. [provided by RefSeq, Jan 2015]