Madd (NM_145527) Mouse Tagged ORF Clone

CAT#: MR212036

Madd (Myc-DDK-tagged) - Mouse MAP-kinase activating death domain (Madd), transcript variant 9

Product Images

Circular map for MR212036

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Madd
• Synonyms: 9630059K23Rik; IG20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR212036 representing NM_145527
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAAAAGAAGTTCTGCCCTCGGTTACTTGACTATCTCGTGATCGTAGGGGCGAGGCACCCAAGCA
  GTGACAGTGTGGCTCAGACTCCTGAGCTGCTGCGGAGGTACCCACTAGAGGATCACCCAGAGTTCCCCCT
  GCCCCCAGATGTGGTGTTCTTCTGCCAGCCAGAAGGATGTCTGAGTGTGCGGCAGCGGCGGATGAGCCTT
  CGGGACGATACCTCTTTTGTCTTCACCCTAACCGATAAGGACACCGGAGTCACCCGCTATGGCATCTGTG
  TCAACTTCTACCGTTCCTTCCAAAAGCGAATGCCAAAGGAAAAGGTGGAAGGCGGAGCAGGACCCCGTGG
  GAAGGAAGGCGCTCACACCTCTGGTGCCTCAGAAGAGGCTGCCGCTGGGAGCTCAGAGAGTGGCTCAACC
  TTGCAGCCGCCTAGTGCTGACTCCACTCCTGACGTAAACCAGTCTCCTCGGGGCAAACGTAGGGCAAAAG
  CGGGCAGCCGCTCCCGCAACAGTACCCTGACATCCCTGTGTGTGCTTAGCCACTACCCTTTCTTCTCTAC
  CTTCAGAGAGTGTCTGTATACTCTCAAACGTTTGGTAGACTGCTGTAGTGAACGGCTGCTAGGCAAGAAA
  CTGGGCATCCCTCGAGGTGTACAAAGGGACACCATGTGGCGAATCTTTACTGGATCACTGCTAGTGGAGG
  AGAAGTCCAGTGCCCTTCTGCAAGACCTTCGAGAGATTGAGGCCTGGATCTATCGGTTGCTACGTTCCCC
  AGTGCCTGTCTCTGGGCAGAAGCGAGTGGACATTGAGGTCCTGCCCCAGGAACTCCAGCAGGCCCTGACA
  TTTGCTCTTCCAGATCCATCTCGATTCACCCTAGTAGATTTCCCTCTTCACCTTCCCTTGGAACTTCTGG
  GTGTAGATGCTTGTCTTCAGGTGCTAACCTGCATCCTGTTGGAGCACAAGGTGGTGCTGCAGTCTCGAGA
  CTACAATGCACTCTCCATGTCTGTGATGGCGTTCGTGGCAATGATCTACCCCCTGGAGTACATGTTCCCG
  GTCATCCCGCTGCTTCCCACCTGCATGGCCTCGGCAGAGCAGCTACTCTTGGCTCCAACCCCATACATCA
  TTGGAGTTCCTGCCAGCTTCTTCCTCTACAAGCTAGATTTCAAAATGCCCGATGACGTGTGGCTAGTGGA
  TCTGGACAGCAATAGGGTGATTGCCCCCACCAATGCAGAAGTGCTACCCATCCTGCCAGAACCAGAATCA
  TTAGAGCTGAAAAAACACTTAAAGCAGGCCTTAGCCAGCATGAGTCTCAACACCCAGCCCATTCTTAACC
  TGGAGAAATTCCACGAGGGCCAGGAGATCCCGCTTCTCTTGGGAAGGCCATCTAATGACCTTCAGTCCAC
  ACCTTCCACTGAATTCAACCCGCTCATTTATGGCAATGATGTAGATTCAGTCGACGTTGCAACGAGAGTG
  GCCATGGTACGTTTCTTCAACTCTGCTAACGTGCTGCAGGGCTTTCAGATGCACACACGTACCCTGCGAC
  TCTTCCCCCGGCCTGTGGTAGCTTTCCAAGCTGGCTCCTTTCTGGCCTCACGTCCCCGGCAGACTCCCTT
  TGCTGAGAAACTGGCCAGGACCCAAGCTGTGGAGTATTTTGGAGAATGGATCCTGAACCCTTCTAACTAT
  GCCTTCCAGCGGATTCACAACAATACGTTTGATCCGGCCCTTATTGGTGACAAGCCAAAGTGGTACGCTC
  ACCAGCTGCAGCCCATCCATTATCGAGTCTATGATGGCAACTCTCAGCTGGCCGAGGCCCTGAGTGTGCC
  CCCGGAGCGCGATTCTGACTCCGACCCGACGGAGGACAGTGGCAGTGATAGTCAGGATTATGATGACTCA
  AGCTCTTCTTACTCCTCCCTTGGGGACTTTGTCAGTGAAATGATGAAATGTGACATCAATGGAGACACTC
  CCAATGTGGATCCTCTGACCCATGCAGCCCTGGGAGATGCCAGTGAAGTGGAGATTGACGAGCTGCAGCC
  CCAGAAGGAGGGAGAGGAACCCGGCCCAGACAGTGAGAACTCTCAGGAAAACCCTCCGCTGCGCTCCAGC
  TCCAGCACCACGGCCAGCAGCAGCCCCAGCACCGTTGTCCACAGTGCCCACTCTGAAGCTGCTGACTCTA
  CAGAGATGGGAGATAAGGCCACAGCAGGCATCTCCAAGCCCCTCCCTCCCGTGCCTCCCAGCATTTGCAA
  ATCTACTGTGGACAGGAGACAGACAGAGACTGGAGAGGGGTCAGTGTGCCAGCGAACCTATGACAATCCA
  TACTTCGAGCCCCAGTATGGCTTTCCCCCCGAGGAAGATGAGGAGGAGCAGGGGGAGAGCTACACTCCCC
  GGTTCAGCCAGCATGTCAGTGGCAGTCGGGCTCAAAAGCTGCTGCGGCCCAACAGCTTGAAGCTGGCAAG
  TGACTCAGACGCAGAGTCAGACTCGAGAGCAAGCTCTCCCAACTCCACTGTCTCTAACAACAGCACCGAG
  GGCTTCGGGGGCATCATGTCTTTTGCTAGCAGCCTGTATCGGAACCATAGTACAAGCTTCAGTCTTTCAA
  ACCTCACACTGCCCACCAAAGGTGCCCGCGAGAAGACGACACCATTCCCCAGCCTGAAAGGAAATAGGAG
  GGCCTTAGTGGACCAGAAGTCATCTGTCATTAAACACAGCCCAACCGTGAAAAGAGAACCTTCATCCCCA
  CAGGGCCGGTCGAGCAACTCTAGCGAGAACCAGCAGTTCCTGAAGGAAGTAGTCCATAGTGTGCTGGACG
  GCCAGGGAGTGGGCTGGCTCAATATGAAGAAGGTACGCCGGCTCCTGGAGAGTGAGCAGCTTAGAGTCTT
  TGTACTGAGCAAGCTGAATCGCGCAGTGCAGTCAGAGGATGACGCCCGGCAGGATGTCATCCAAGACGTG
  GAGATCAGCCGGAAGGTGTACAAGGGGATGCTAGACCTCCTCAAGTGCACAGTCCTCAGCCTCGAGCAGT
  CCTATGCCCACGCAGGTCTGGGTGGTATGGCCAGCATCTTTGGGCTTTTGGAGATTGCCCAGACCCACTA
  CTATAGTAAAGAACCAGACAAACGGAAGAGAAGTCCGACAGAGAATGTAAATACCCCAGTTGGCAAGGAT
  CCTGGTCTGGCTGGGCGGGGGGACCCAAAGGCAATGGCCCAGCTAAGGGTCCCTCAACTGGGCCCTCGGG
  CACCAAGTGCTACAGGAAAGGGTCCTAAAGAACTGGATACCAGAAGTTTAAAGGAAGAGAATTTTGTAGC
  ATCTGTTGAATTGTGGAACAAGCACCAGGAAGTGAAAAAGCAAAAGGCTTTGGAAAAACAGAGGCCAGAG
  GGCATCAAGCCTGTCTTTGACCTGGGTGAGACAGAGGAGAAAAAGTCCCAGATGAGCGCAGACAGTGGTG
  TGAGCCTGACATCTGCCTCCCAGAGGACTGATCAAGACTCTGTCATCGGTGTGAGTCCAGCTGTTATGAT
  CCGAAGCTCAAGTCAGGATTCTGAAGTTAGCACCGTGGTGAGTAATAGTTCCGGAGAGACCCTTGGAGCT
  GACAGTGACCTGAGCAGCAATGCAGGTGACGGGCCAGGAGGCGAAGGCAGCGCCCACCTGGCAAGTTCCC
  GGGCCACTCTGTCCGACAGTGAAATCGAAACCAATTCTGCTACAAGCGCCATCTTTGGTAAAGCCCATAG
  CTTGAAGCCAAAGGAGAAGCCGGCTGGTAGTCCGATTCGCTCTTCCGAAGACGTAAGCCAGCGAGTCTAT
  CTCTATGAGGGACTGCTAGGAAGGGACAAAGGATCCATGTGGGACCAGCTAGAGGATGCCGCTATGGAGA
  CCTTTTCTCTGAGCAAAGAACGTTCTACCTTATGGGACCAAATGCAGTTCTGGGAAGATGCATTCTTAGA
  TGCTGTAATGTTGGAAAGAGAAGGAATGGGTATGGACCAGGGTCCTCAGGAAATGATCGACAGGTACCTC
  TCCCTAGGGGAACACGACCGGAAGCGCCTGGAGGATGATGAAGACCGCTTGCTGGCCACACTTTTACACA
  ACCTCATATCCTATATGCTGCTGATGAAGGTGAACAAGAATGACATCAGGAAGAAAGTACGGCGCCTAAT
  GGGAAAGTCCCACATTGGGCTGGTGTACAGCCAGCAAGTCAATGAGGTGCTCGATCAGCTGAACAGCCTG
  AATGGACGTGATCTCTCCATCCGGTCCAGCGGCAGCCGGCACATGAAGAAGCAGACTTTTGTTGTGCATG
  CAGGGACGGACACAAATGGAGACATCTTTTTCATGGAAGTGTGTGATGACTGCGTGGTGTTGCGTAGTAA
  CATTGGGACGGTGTACGAGCGCTGGTGGTATGAGAAGCTCATCAATATGACCTACTGTCCCAAGACCAAG
  GTCCTGTGCTTATGGCGGAGAAATGGCTCTGAGACCCAGCTCAACAAGTTCTATACCAAGAAGTGTCGAG
  AGCTGTACTACTGTGTGAAGGACAGCATGGAACGTGCTGCTGCCCGACAGCAAAGCATCAAACCTGGGCC
  TGAGCTAGGCGGTGAGTTCCCTGTGCAGGACATGAAGACTGGAGAGGGTGGCTTGCTTCAAGTCACCCTG
  GAAGGGATCAATCTCAAGTTCATGCACAACCAGTTCCTGAAATTAAAGAAGTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR212036 representing NM_145527
  Red=Cloning site Green=Tags(s)
  
  MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSL
  RDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGST
  LQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKK
  LGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALT
  FALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFP
  VIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPES
  LELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRV
  AMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNY
  AFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDS
  SSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSS
  SSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNP
  YFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTE
  GFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSP
  QGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDV
  EISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKD
  PGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPE
  GIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGA
  DSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVY
  LYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYL
  SLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSL
  NGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTK
  VLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTL
  EGINLKFMHNQFLKLKKW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145527
• ORF Size: 4674 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145527.4, NP_663502.3
• RefSeq Size: 6094 bp
• RefSeq ORF: 4677 bp
• Locus ID: 228355
• UniProt ID: Q80U28
• Cytogenetics: 2 E1
• MW: 173.7 kDa
• Gene Summary: Plays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis).[UniProtKB/Swiss-Prot Function]