Arhgef11 (NM_001003912) Mouse Tagged ORF Clone

CAT#: MR212032

Arhgef11 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 11 (Arhgef11)

Product Images

Circular map for MR212032

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arhgef11
• Synonyms: B930073M02; E130307F09; mKIAA0380; PDZ-RhoGEF; Prg; RhoGEF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR212032 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCATACGGTTGCCCCATAGTATAGACAGGTTAAGTAGCCTGTCTTCTCTGGGAGATTCTACACCTG
  AACGCACATCCCCTTCTCACCACCGCCAGCCCTCTGACACCTCTGAGACAACAGGTCTTGTTCAGCGCTG
  TGTCATCATCCAAAAGGACCAGCATGGCTTTGGCTTCACAGTTAGTGGAGATCGCATTGTGCTGGTGCAG
  TCTGTGCGCCCTGGAGGCGCAGCCATGAGAGCTGGTGTGAAAGAGGGTGACCGGATCATCAAAGTAAACG
  GCACCATGGTGACCAATAGCTCACACCTGGAGGTAGTGAAGCTTATCAAATCTGGCGCCTATGTTGCGCT
  TACCCTCCTGGGCTCTTCTCCTTCCTCCATCGGTGTCTCTGGGCTCCAGCAGAACCCTTCTCTGTCAGGA
  GGGCCCAGAGTTAACCCCATGATTCCTCCACCACCTCCTCCTCCACCCTTGCCACCTCCACAACACATTA
  CTGGACCTAAACCTCTACAGGATCCTGAAGTCCAAAAGCACGCCACTCAAATCCTCAGGAATATGCTAAG
  GCAGGAGGAGAAGGAGTTACAGCGTATCTGTGAGGTGTATAGTCGGAACCCCGCCAGCCTGCTAGAAGAG
  CAAATCGAAGGTGCCCGGCGGCGAGTCACTCAATTACAGCTGAAGATCCAGCAGGAGACTGGTGGCTTAG
  TGGATGTACTTCCACTCTGTGGTGAGACCAGCCAGAGAACATGTGAAGGCCGCCTCTCTGTGGACTCCCA
  GGAGGCAGACAGTGGCTTGGACTCAGGGACAGAACGCTTTCCCTCCATCAGTGAGTCATTGGTGAATCGG
  AACTCAGTATTGTCAGATCCTGGACTAGACAGCCCACAAACCTCCCCTGTAATCCTGGCCAGGGTGGGCC
  AGCACCACAGGCGACAGGGCTCAGATGCAGCGGTGCACCCCCTCAACCACCAGGGTATAGATCAAAGCCC
  AAAGCCTCTGATTATTGGCCCAGAGGAAGATTATGACCCAGGCTATTTCAACAATGAGAGTGACATCATC
  TTTCAAGATCTTGAAAAACTGAAGTCACATCCAGCTTACTTGGTAGTATTTCTACGTTACATCTTCTCTC
  AGGCAGACCCCGGCCCCCTGCTTTTTTATTTGTGTTCAGAAGTTTATCAGCAGACAAATCCCAAAGATTC
  CCGAAATCTGGGGAAAGACATCTGGAATATTTTCCTAGAGAAAAATGCGCCTCTCAGAGTGAAGATCCCT
  GAGATGTTGCAGGCTGAGATCGACCTACGCCTGCGAAACAGTGAGGACCCTCGCAGCGCGCTTTATGAAG
  CTCAGGAGGCAGTCATGCCGGAGATCCAGGAGCAGATCAATGACTATAGATCAAAGCGCACTCTGGGGCT
  GGGCAGCCTCTATGGTGAAAATGACCTACTAGACCTGGATGGGGACCCTCTTCGAGAGCGCCAAATGGCT
  GAGAAGCAACTGGCTGCCCTTGGAGATATCTTGTCCAAATATGAGGAAGATCGGAGTGCTCCCATGGACT
  TCGCTGTTAATACCTACATGAGCCACACTGGGATCCGTCTTCGGGAGTCTCGACCTTCCAGCACAGCAGA
  AAAGACCCAGTCTGCCCCCGACAAGGACAAGTGGCTGCCCTTCTTCCCTAAGACCAAGAAGAGCAGCAAT
  TCCAAGAAAGAAAAGGACGCCTTGGAGGACAAGAAGCGAAACCCCATCCTCAGATACATTGGGAAGCCCA
  AGAGCTCTTCTCAGAGCATTAAGCCAGGCAATGTGAGGAACATCATTCAGCACTTTGAGAACAGCCATCA
  GTATGATGTCCCAGAGCCGGGGACACAGCGACTCTCAACAGGAAGCTTTCCTGAGGACCTGCTGGAGAGT
  GACAGTTCACGCTCAGAGATTCGCCTTGGCCGCTCTGAAAGCCTCAAGGGCCGGGAGGAGATGAAGCGAT
  CCCGGAAGGCAGAGAATGTGCCCCGGTCTCGAAGTGATGTTGACATGGATGCTGCTGCTGAGGCTACTCG
  CCTTCATCAGTCAGCCTCATCCTCTGCCTCCAGCCTCTCCACCAGGTCTCTTGAGAACCCAACCCCTCCC
  TTCACCCCCAAAATGGGCCGCAGGAGCATTGAGTCCCCCAATCTGGGGTTCTGTACAGATGTCATCCTTC
  CCCACCTCCTTGAGGATGATCTGGGCCAACTGTCTGACCTGGAGCCAGAGCCAGAAGTCCAAAACTGGCA
  GCATACAGTGGGCAAGGATGTGGTGGCAAACTTGACACAGAGGGAAATTGACCGGCAAGAGGTCATCAAT
  GAGCTCTTTGTGACAGAAGCATCCCACCTGCGCACGCTACGAGTCCTGGACCTCATCTTCTACCAGCGCA
  TGAAGAAGGAGAACCTAATGCCACGGGAAGAGCTGTCAAGGCTCTTCCCTAATCTGCCTGAACTCATAGA
  GATCCACAATTCCTGGTGTGAAGCCATGAAGAAGCTCCGGGAGGAGGGCCCCATTATCAGAGACATCAGT
  GACCTCATGCTGGCTCGGTTTGATGGTCCTGCCCGAGAAGAGCTCCAGCAAGTAGCTGCACAATTCTGTT
  CCTATCAGTCAGTAGCCCTGGAGCTAATCAGGACTAAACAACGCAAGGAGAGTCGATTCCAGCTCTTCAT
  GCAGGAAGCCGAGAGCCACCCCCAGTGTCGGCGTCTGCAGCTTCGTGACCTGATCATCTCTGAGATGCAG
  CGGCTCACTAAGTACCCACTGCTGCTAGAGAACATCATCAAGCACACAGCGGGTGGCACATCTGAGTATG
  AGAAGCTCTGCCGTGCCCGGGACCAGTGCCGAGAGATTCTCAAGTTTGTGAATGAAGCAGTGAAGCAGAC
  AGAGAACCGTCACCGCTTAGAAGGGTACCAGAAACGCCTGGATGCCACTGCTCTAGAGCGGGCCAGCAAC
  CCCTTGGCAGCAGAGTTCAAGAGCCTGGATCTTACGACAAGGAAGATGATCCACGAGGGGCCTCTGACCT
  GGAGGATCAGCAAAGATAAGACCCTGGACCTCCAGGTGCTGTTACTCGAGGACCTGGTAGTGCTGCTGCA
  GAGACAAGAGGAGAAGCTGCTGCTCAAGTGCCACAGCAAGACAGCTGTGGGCTCCTCAGACAGCAAGCAG
  ACCTTCAGCCCTGTGCTGAAGCTCAATGCCGTGCTCATCCGCTCTGTGGCCACAGATAAACGAGCCTTCT
  TCATCATCTGCACCTCCGAGCTGGGCCCTCCCCAGATCTATGAGCTGGTTGCATTGACATCATCAGACAA
  GAATATATGGATGGAGCTCTTAGAAGAGGCTGTGCAGAATGCCACCAAGCACCCTGGAGATGCCCCAGTA
  CTCAACCATCCCTCACCACCAGGATCCCAGGAGCCAGCCTACCAGGGCTCCACCTCCAGCAGGGTAGAGG
  TGAATGACTCGGAAGTGTATCCCACTGAAAGAGAACCCAAGAAGCCATCTGAAGGCCCCGGGCCCGAGCA
  GAGAGGTCAAGACAAGCAGCTGCTAGCACAAGAGGGGCCTGAGCAGGAGGAGGATGCAGAGGAGCTGAGG
  GCCCTGCCTTGCCCTCCTCCCTCCCTGGATGGAGAAAACAGAGGCATCAGGACAAGGGACCCTGTCCTTC
  TGGCCCTCACAGGCCCTCTGCTCATGGAGGGACTTGCTGATGCTGCCCTGGAAGATGTGGAGAACTTGCG
  GCACCTTATCCTGTGGAGCCTGCTGCCTGGTCACACTGTGAAGACTCAGGCTGCTGGCGAGCCTGAAGAT
  GACCTTACACCCACCCCTTCTGTCGTCAGCATTACCTCTCACCCCTGGGACCCAGGATCCCCAGGGCAAG
  CTCCTGCTATAAGTGACAACACCCAATTTCCAAGGCCAGAGGGAAGCCAGCCAGAGGGCGAGGATGTTGC
  TCTCTGTTCTCTGGCACACTTGCCACCAAGGACCAGAAATTCTGGCATCTGGGACTCTCCTGAGCTGGAT
  AGGAATCCAGCTGAAGAGGCTTCAAGCTCAGAACCAGCAGGAAGTTACAAAGTTGTAAGGAAAGTCTCTC
  TACTCCCTGGTGGTGGTGTCGGTGCAGCCAAGGTGGCGGGCAGCAATGTTACCCCTGCACTCCCAGAGAG
  TGGCCAGTCAGAATCTGAGCTATCTGAAGTGGAAGGCGGAGCACAGGCTACGGGGAACTGTTTCTATGTC
  AGCATGCCAGCAGAACCTCTGGACTCTAGCACTGAGCCTCCTGGGACACCCCCAAGTCTCTCGCAGTGTC
  ACAGCCTCCCTGCATGGCCAACAGAGCCTCCGCAACACAGGGGAGTCACTGGGGGTCAGCGTTCCAGCCT
  GGTCCTCAGGGATATGGGTGTGATCTTCCACACCATCGAGCAACTGACCGTCAAGCTTCACAGACTCAAG
  GACATGGAGCTGGCCCACAGAGAGCTGCTCAACTCCCTTGGAGGAGAGTCGTCTGGTGGAACCACTCCTG
  TGGGAAGTTTTCACACAGAGGCAGCCAGATGGACAGACTACTCCCTCTCTCCTCCAGCCAAGGAAGCCCT
  GACCTCTGACCCCCAAAACAATCAGGAGCAGGGGTCCTACCCTGAAGAAGGCTCCGACACCCCCCTGGAA
  GACAGTGCCACAGACACAGCTTCGTCACCAGGACCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR212032 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSIRLPHSIDRLSSLSSLGDSTPERTSPSHHRQPSDTSETTGLVQRCVIIQKDQHGFGFTVSGDRIVLVQ
  SVRPGGAAMRAGVKEGDRIIKVNGTMVTNSSHLEVVKLIKSGAYVALTLLGSSPSSIGVSGLQQNPSLSG
  GPRVNPMIPPPPPPPPLPPPQHITGPKPLQDPEVQKHATQILRNMLRQEEKELQRICEVYSRNPASLLEE
  QIEGARRRVTQLQLKIQQETGGLVDVLPLCGETSQRTCEGRLSVDSQEADSGLDSGTERFPSISESLVNR
  NSVLSDPGLDSPQTSPVILARVGQHHRRQGSDAAVHPLNHQGIDQSPKPLIIGPEEDYDPGYFNNESDII
  FQDLEKLKSHPAYLVVFLRYIFSQADPGPLLFYLCSEVYQQTNPKDSRNLGKDIWNIFLEKNAPLRVKIP
  EMLQAEIDLRLRNSEDPRSALYEAQEAVMPEIQEQINDYRSKRTLGLGSLYGENDLLDLDGDPLRERQMA
  EKQLAALGDILSKYEEDRSAPMDFAVNTYMSHTGIRLRESRPSSTAEKTQSAPDKDKWLPFFPKTKKSSN
  SKKEKDALEDKKRNPILRYIGKPKSSSQSIKPGNVRNIIQHFENSHQYDVPEPGTQRLSTGSFPEDLLES
  DSSRSEIRLGRSESLKGREEMKRSRKAENVPRSRSDVDMDAAAEATRLHQSASSSASSLSTRSLENPTPP
  FTPKMGRRSIESPNLGFCTDVILPHLLEDDLGQLSDLEPEPEVQNWQHTVGKDVVANLTQREIDRQEVIN
  ELFVTEASHLRTLRVLDLIFYQRMKKENLMPREELSRLFPNLPELIEIHNSWCEAMKKLREEGPIIRDIS
  DLMLARFDGPAREELQQVAAQFCSYQSVALELIRTKQRKESRFQLFMQEAESHPQCRRLQLRDLIISEMQ
  RLTKYPLLLENIIKHTAGGTSEYEKLCRARDQCREILKFVNEAVKQTENRHRLEGYQKRLDATALERASN
  PLAAEFKSLDLTTRKMIHEGPLTWRISKDKTLDLQVLLLEDLVVLLQRQEEKLLLKCHSKTAVGSSDSKQ
  TFSPVLKLNAVLIRSVATDKRAFFIICTSELGPPQIYELVALTSSDKNIWMELLEEAVQNATKHPGDAPV
  LNHPSPPGSQEPAYQGSTSSRVEVNDSEVYPTEREPKKPSEGPGPEQRGQDKQLLAQEGPEQEEDAEELR
  ALPCPPPSLDGENRGIRTRDPVLLALTGPLLMEGLADAALEDVENLRHLILWSLLPGHTVKTQAAGEPED
  DLTPTPSVVSITSHPWDPGSPGQAPAISDNTQFPRPEGSQPEGEDVALCSLAHLPPRTRNSGIWDSPELD
  RNPAEEASSSEPAGSYKVVRKVSLLPGGGVGAAKVAGSNVTPALPESGQSESELSEVEGGAQATGNCFYV
  SMPAEPLDSSTEPPGTPPSLSQCHSLPAWPTEPPQHRGVTGGQRSSLVLRDMGVIFHTIEQLTVKLHRLK
  DMELAHRELLNSLGGESSGGTTPVGSFHTEAARWTDYSLSPPAKEALTSDPQNNQEQGSYPEEGSDTPLE
  DSATDTASSPGP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001003912
• ORF Size: 4659 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003912.2
• RefSeq Size: 5617 bp
• RefSeq ORF: 4659 bp
• Locus ID: 213498
• Cytogenetics: 3 F1
• MW: 171.8 kDa