Kdm5b (NM_152895) Mouse Tagged ORF Clone

CAT#: MR212028

Kdm5b (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 5B (Kdm5b)

Product Images

Circular map for MR212028

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kdm5b
• Synonyms: 2010009J12Rik; 2210016I17Rik; AW556288; D1Ertd202; D1Ertd202e; Jari; Jarid1b; mKIAA4034; Pl; PLU; PLU-1; Plu1; PUT1; Rb-B; Rb-Bp2; RBBP2H1A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR212028 representing NM_152895
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGGCCACCACGCTGCCCCCAGGCCCGCGCCCGGCGCTGCCCCTCGGGGGCCCGGGCCCGCTGG
  GAGAGTTCCTGCCTCCCCCCGAGTGTCCGGTCTTCGAGCCGAGCTGGGAAGAGTTCGCGGACCCCTTCGC
  TTTCATCCACAAGATCCGGCCCATAGCCGAGCAGACTGGGATCTGTAAGGTGCGGCCGCCGCCGGATTGG
  CAGCCACCATTCGCTTGTGATGTCGATAAACTTCATTTCACCCCGCGGATCCAGAGGCTGAATGAGCTGG
  AGGCCCAAACACGTGTAAAATTGAATTTCCTGGACCAGATTGCAAAGTATTGGGAGCTACAAGGAAGTAC
  TCTGAAGATTCCCCACGTGGAGAGAAAGATCTTAGACTTGTTCCAGCTAAATAAGTTAGTCGCAGAAGAA
  GGTGGGTTTGCAGTTGTTTGCAAGGATAGAAAATGGACCAAAATTGCCACCAAGATGGGTTTTGCTCCTG
  GTAAAGCTGTGGGCTCACATATCAGGGGACATTATGAGCGAATACTCAATCCATACAATTTGTTCCTGTC
  TGGAGACAGTCTGAGGTGTCTTCAGAAGCCTAACCTGACCTCCGACACAAAGGACAAGGAGTACAAACCC
  CATGATATTCCCCAGAGGCAGTCTGTGCAGCCCGCAGAAACCTGTCCTCCGGCCCGCCGAGCAAAGCGCA
  TGAGAGCAGAGGCTATGAATATTAAAATTGAGCCAGAAGAGGCAACAGAAGCCAGGACTCATAATCTGAG
  ACGCCGAATGGGTTGCACAACTCCAAAATGGGAAAATGAGAAAGAAATGAAAAGCACCATCAAGCAAGAG
  CCCACTGAGAAGAAAGACTGCGAGTTAGAAAGTGAGAAGGAGAAGCCCAAGAGCCGGGCCAAGAAAACGG
  CCACTGCGGTGGACCTGTATGTGTGCCTCTTATGTGGAAGTGGCAATGACGAGGACCGACTCCTACTGTG
  TGACGGCTGTGATGACAGTTACCACACCTTCTGCCTGGTTCCCCCTCTCCATGACGTTCCCAAGGGAGAC
  TGGAGGTGTCCTAAGTGTTTGGCTCAAGAATGTAATAAGCCACAAGAAGCTTTTGGTTTTGAACAAGCAG
  CAAGGGACTATACACTTCGTACATTTGGAGAAATGGCAGATGCATTCAAATCTGATTACTTCAACATGCC
  AGTCCATATGGTTCCCACAGAGCTGGTGGAGAAGGAGTTTTGGCGGCTCGTAAGCACTATTGAAGAAGAC
  GTCACGGTGGAGTATGGAGCTGATATTGCTTCAAAAGAGTTTGGCAGTGGCTTTCCTGTTCGAGATGGGA
  AAATCAAGATTTCTCCTGAGGAAGAGGAATACCTGGATAGTGGCTGGAATTTGAACAACATGCCAGTGAT
  GGAGCAGTCTGTCCTTGCTCATATTACTGCTGATATATGTGGCATGAAACTCCCCTGGTTGTATGTGGGC
  ATGTGCTTTTCTTCATTCTGTTGGCACATTGAAGACCACTGGAGCTATTCAATCAACTACCTACACTGGG
  GTGAGCCAAAAACCTGGTATGGAGTTCCAGGCTACGCTGCTGAGCAGCTAGAAAACGTGATGAAGAAGCT
  CGCTCCTGAGCTCTTTGTGTCCCAGCCGGACCTGCTCCACCAGCTGGTGACCATCATGAACCCCAACACT
  CTGATGACTCATGAAGTGCCTGTTTATCGAACGAATCAGTGTGCTGGGGAGTTTGTAATTACGTTTCCAA
  GAGCCTACCACAGTGGCTTCAACCAAGGTTTTAATTTTGCCGAGGCTGTTAACTTCTGCACTGTTGATTG
  GCTGCCATTGGGCCGCCAGTGTGTGGAGCATTATCGCTTGCTGCACCGTTATTGTGTCTTTTCCCACGAT
  GAAATGATATGCAAAATGGCTTCTAAGGCTGATGTATTAGACGTTGTGGTAGCGTCAACTGTTCAGAAGG
  ACATGGCCATCATGATCGAGGACGAGAAAGCTTTAAGGGAAACTGTTCGGAAATTGGGAGTAATTGATTC
  TGAAAGAATGGATTTTGAGCTGTTGCCAGATGATGAGCGGCAGTGTATAAAATGCAAAACTACATGCTTC
  ATGTCTGCCATCTCCTGCTCTTGTAAACCTGGCCTACTTGTTTGCCTACATCATGTGAAAGAATTGTGTT
  CTTGCCCCCCGTATAAGTATAACCTGCGGTATAGATACACTCTGGATGATCTCTATCCAATGATGAATGC
  ATTAAAGCTTCGAGCAGAGTCTTACAATGAATGGGCCTTAAATGTGAATGAAGCCTTGGAAGCAAAGATC
  AATAAGAAGAAAAGTCTTGTCAGCTTTAAAGCTTTGATTGAAGAATCAGAAATGAAAAAATTCCCAGACA
  ATGATCTTTTGCGTCACCTTCGACTAGTCACGCAGGATGCAGAAAAGTGTGCCTCTGTTGCCCAGCAGTT
  GCTTAATGGCAAAAGGCAAACAAGGTATCGATCTGGTGGAGGAAAGTCTCAGAATCAGCTGACGGTGAAT
  GAACTTAGACAGTTTGTGACTCAGCTGTATGCTCTGCCGTGCGTCCTTAGTCAGACACCGTTGCTAAAGG
  ATCTCTTGAATCGTGTGGAAGATTTCCAACAGCAAAGCCAAAAACTACTGTCTGAGGAAATGCCTAGCGC
  TGCCGAGCTACAGGAGTTGCTGGATGTCAGCTTTGAATTTGATGTTGAGCTTCCACAGCTCACTGAGATG
  CGCATTCGCTTAGAGCAGGCCCGCTGGCTGGAAGAGGTTCAGCAGGCTTGCCTGGACTCCAGCTCCCTTT
  CTTTGGATGATATGAGACGACTCATAGACTTAGGGGTGGGGCTGGCTCCATATTCTGCTGTGGAGAAAGC
  TATGGCCCGCCTGCAGGAGCTGCTAACAGTGTCAGAACACTGGGACGACAAAGCCAAGAGTCTCCTCAGA
  GCAAGACCACGGCATTCTTTGAGTAGCCTTGCTACAGCAGTCAAAGAGATGGAGGAGATCCCCGCCTACC
  TGCCCAATGGTACAGTCCTGAAGGATTCTGTGCAAAGAGCCAGAGACTGGGTTCAGGATGTGGATGCATT
  GCAGGCAGGAGGACGTGTGCCAGTGTTAGAGACACTCATTGAACTTGTTGCAAGAGGCCGATCTATCCCA
  GTACATCTCAATTCTTTACCAAGATTGGAAATGTTAGTAGCTGAAGTTCATGCATGGAAAGAATGTGCTG
  CTAAAACATTCTTGCCTGAGAATTCTACATATTCTCTGTTAGAGGTTCTTTGCCCTCGGTGTGACATTGG
  CCTTTTGGGGCTGAAAAGGAAACAGAGAAAGTTGAAGGAGCCCTTGCCGAGTGGAAAGAAGAGAAGCACC
  AAGTTAGAGAGCCTGAGCGATCTGGAGAGGGCTCTGATGGAGAGCAAAGAGACTGCGGCAGCTATGGCAA
  CTCTTGGGGAAGCTCGCCTACGAGAGATGGAAGCTTTGCAGTCTCTCAGATTTGCCAATGAAGAGAAATT
  GCTGTCACCTGTCCAAGACTTAGAGATGAAAGTCTGCTTGTGTCAGAAGACTCCAGCCACTCCCATGATT
  CAGTGTGAACTCTGCAGGGATGCTTTCCATACTAGTTGTGTGGCAGCACCCAGTATTTCACAAAGCTCCC
  GAATCTGGCTTTGTCCTCACTGTCGGAGGTCAGAGAAGCCTCCATTAGAGAAAATTCTGCCTCTGCTGGC
  CTCCCTACAGCGTATACGAGTTCGTCTTCCTGAAGGAGATGCACTCCGATACATGATTGAAAGAACCGTG
  AACTGGCAACATAGAGCCCAGCAGCTGCTTTCTTCAGGGAATCTAAAACTTGTGCAAGACCAAGTGGGTT
  CTGGACTGTTGTCTAGCAGATGGCCAGCCTCAGCAGGACAGGCATCAGCAACAGACAAGGTGTCTCAGCC
  TCCTGGCACAACATCCTTTTCATTGCCCGATGACTGGGACAACAGAACCTCATATTTACACTCTCCCTTC
  TCTACTGGACAGAGTTGTCTCCCCCTCCATGGTTTGAGTCCAGAAGTGAATGAATTGCTGATGGAAGCCC
  AGCTGCTCCAGGTATCCCTTCCTGAAATTCAGGAGCTTTATCAGACTCTACTTACTAAGCCAAGCTCTGT
  TCAGCAAGCTGACCGAAGTTCACCAGTTAGATCCAGCAGTGAGAAGAACGATTGCCTTCGAGGGAAACGA
  GATGCAATCAACAGTCCTGAGAGGAAACTGAAAAGGCGCCCAGAAAGAGAAGGCCTTCCTAGCGAGCGGT
  GGGATCGAGTTAAACACATGCGGACCCCCCAAAAGAAGAAAATCAAACTGAGTCACCCCAAGGACATGGA
  CAGTTTCAAGTTAGAAAGAGAGCGTAGCTATGACTTAGTGCGTAATGCTGAAACTCATTCCCTGCCCTCA
  GACACATCCTACTCCGAACAGGAGGACTCTGAGGATGAAGATGCCATCTGTCCAGCTGTGAGCTGCCTGC
  AGCCAGAAGGAGATGAGGTGGACTGGGTCCAGTGTGATGGCAGCTGCAATCAGTGGTTTCACCAGGTCTG
  TGTTGGTGTCTCTCCAGAGATGGCAGAGAAGGAAGACTACATCTGTGTGCGCTGTACTGGGAAGGACGCA
  CCAAGCCGAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR212028 representing NM_152895
  Red=Cloning site Green=Tags(s)
  
  MEPATTLPPGPRPALPLGGPGPLGEFLPPPECPVFEPSWEEFADPFAFIHKIRPIAEQTGICKVRPPPDW
  QPPFACDVDKLHFTPRIQRLNELEAQTRVKLNFLDQIAKYWELQGSTLKIPHVERKILDLFQLNKLVAEE
  GGFAVVCKDRKWTKIATKMGFAPGKAVGSHIRGHYERILNPYNLFLSGDSLRCLQKPNLTSDTKDKEYKP
  HDIPQRQSVQPAETCPPARRAKRMRAEAMNIKIEPEEATEARTHNLRRRMGCTTPKWENEKEMKSTIKQE
  PTEKKDCELESEKEKPKSRAKKTATAVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLVPPLHDVPKGD
  WRCPKCLAQECNKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVHMVPTELVEKEFWRLVSTIEED
  VTVEYGADIASKEFGSGFPVRDGKIKISPEEEEYLDSGWNLNNMPVMEQSVLAHITADICGMKLPWLYVG
  MCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPGYAAEQLENVMKKLAPELFVSQPDLLHQLVTIMNPNT
  LMTHEVPVYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCVEHYRLLHRYCVFSHD
  EMICKMASKADVLDVVVASTVQKDMAIMIEDEKALRETVRKLGVIDSERMDFELLPDDERQCIKCKTTCF
  MSAISCSCKPGLLVCLHHVKELCSCPPYKYNLRYRYTLDDLYPMMNALKLRAESYNEWALNVNEALEAKI
  NKKKSLVSFKALIEESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVN
  ELRQFVTQLYALPCVLSQTPLLKDLLNRVEDFQQQSQKLLSEEMPSAAELQELLDVSFEFDVELPQLTEM
  RIRLEQARWLEEVQQACLDSSSLSLDDMRRLIDLGVGLAPYSAVEKAMARLQELLTVSEHWDDKAKSLLR
  ARPRHSLSSLATAVKEMEEIPAYLPNGTVLKDSVQRARDWVQDVDALQAGGRVPVLETLIELVARGRSIP
  VHLNSLPRLEMLVAEVHAWKECAAKTFLPENSTYSLLEVLCPRCDIGLLGLKRKQRKLKEPLPSGKKRST
  KLESLSDLERALMESKETAAAMATLGEARLREMEALQSLRFANEEKLLSPVQDLEMKVCLCQKTPATPMI
  QCELCRDAFHTSCVAAPSISQSSRIWLCPHCRRSEKPPLEKILPLLASLQRIRVRLPEGDALRYMIERTV
  NWQHRAQQLLSSGNLKLVQDQVGSGLLSSRWPASAGQASATDKVSQPPGTTSFSLPDDWDNRTSYLHSPF
  STGQSCLPLHGLSPEVNELLMEAQLLQVSLPEIQELYQTLLTKPSSVQQADRSSPVRSSSEKNDCLRGKR
  DAINSPERKLKRRPEREGLPSERWDRVKHMRTPQKKKIKLSHPKDMDSFKLERERSYDLVRNAETHSLPS
  DTSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGVSPEMAEKEDYICVRCTGKDA
  PSRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152895
• ORF Size: 4632 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152895.2, NP_690855.2
• RefSeq Size: 6300 bp
• RefSeq ORF: 4635 bp
• Locus ID: 75605
• UniProt ID: Q80Y84
• Cytogenetics: 1 58.24 cM
• MW: 176 kDa
• Gene Summary: This gene encodes a lysine-specific histone demethylase that belongs to the jumonji/ARID domain-containing family of histone demethylases. The encoded protein is capable of demethylating tri-, di- and monomethylated lysine 4 of histone H3. This protein plays a role in the transcriptional repression or certain tumor suppressor genes and is upregulated in certain cancer cells. This protein may also play a role in genome stability and DNA repair. Homozygous mutant mice display decreased body weight, decreased female fertility, lower uterine weight, and a delay in mammary development. Knockout of this gene has also been associated with embryonic lethality. [provided by RefSeq, Dec 2016]