Setd5 (BC083184) Mouse Tagged ORF Clone

CAT#: MR211996

Setd5 (Myc-DDK-tagged) - Mouse SET domain containing 5 (cDNA clone MGC:90828 IMAGE:30536831)

Product Images

Circular map for MR211996

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Setd5
• Synonyms: mKIAA1757, C330007C20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211996 representing BC083184
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCATTGCAATCCCTCTGGGAGTCACCACACCAGATACTTCCTACTCAGATATGGCTGCTGGATCAG
  ACCCTGAATCTGTGGAGGCTAGTCCAGCAGTTAATGAGAAGAGCGTGTATTCCACTCATAATTATGGGAC
  CACTCAGAGGCATGGGTGTCGAGGACTGCCTTATGCTGATCATAATTACGGAGCCCCTCCTCCTCCGACA
  CCTCCTGCTTCTCCCCCTGTCCAGACGATCATCCCTCGTTCTGACCTGAATGGCCTGCCGTCGCCCGTAG
  AGGAACGCTGTGGAGACAGCCCGAACTCTGAAGGAGAGACTGTTCCTACCTGGTGTCCTTGTGGTCTTTC
  TCAGGATGGCTTCCTTCTCAACTGTGACAAGTGCAGGGGAATGAGCAGGGGGAAGGTTATTAGACTTCAT
  CGGCGGAAGCAGGACAACATATCAGGTGGGGATAGCAGTGCAACAGAAAGCTGGGATGAGGAGCTTTCTC
  CTTCCACTGTGTTGTACACAGCAACACAGCACACACCTACAAGCATCACCTTAACTGTTAGAAGAACCAA
  ACCCAAGAAGCGGAAAAAGAGTCCAGAAAAGGGTCGTGCAGCACCAAAGACGAAGAAGATCAAGAATTCT
  CCCTCGGAAGCACAGAATTTAGATGAAAATACCACTGAGGGCTGGGAAAATCGGATAAGACTATGGACTG
  ATCAATATGAAGAAGCTTTCACTAATCAGTACAGTGCAGATGTACAGAATGCCCTTGAGCAACATCTCCA
  TTCTAACAAGGAATTTGTGGGCAAACCTGCAATTTTAGACACTATTAATAAAACTGAACTGGCCTGTAAT
  AATACAGTAATCGGTTCCCAAATGCAGTTACAGCTGGGAAGAGTCACTCGTGTTCAAAAGCACCGGAAAA
  TCCTGAGGGCTGCAAGAGATTTGGCTTTGGACACTCTTATAATAGAATATCGTGGGAAAGTCATGTTACG
  ACAACAATTTGAGGTCAATGGGCATTTCTTTAAAAAACCATATCCCTTTGTGCTCTTCTACTCAAAATTC
  AATGGTGTAGAGATGTGTGTCGATGCTCGTACTTTCGGTAATGATGCTCGGTTCATCAGAAGATCATGTA
  CACCAAATGCAGAGGTGAGACACATGATTGCAGATGGGATGATTCACTTGTGTATCTATGCCGTATCTGC
  TATCACCAAGGATGCAGAAGTCACCATTGCATTTGATTATGAATATAGTAACTGTAATTACAAAGTGGAC
  TGTGCTTGTCACAAGGGAAACCGGAATTGCCCTATACAGAAAAGAAATCCCAATGCTGCAGAATTGCCAC
  TCCCACCTCCTCCTAGCTTTCCCACCATTGGAGCAGAGACCAGACGTAGAAAAGCACGGCGGAAAGAGCT
  GGAGCTGGAGCAGCAAAATGAGGTTCCAGAAGAGAATCCTGACCCGCAACCACAAGAAGTTCCAGAAAAA
  GTAACTGTATCCAATGAGCATGAGGAAGTTGACAATCCAGAAGAAAAACCAGAAGAAGAAGAAAAAGAAG
  AGGCTACAGATGACCAGGAGAACTCTGCTCATAGCAGAAGGACTCGAGAAGACCGAAAGGTTGAAGCCAT
  CATGCATGCTTTTGAATCTTTAGAGAAGAGAAAGAAACGGCGGGATCAGCCTGTAGAACAGAGCAGCTCG
  GACATAGAGATTACTACTAGCAGTTCAGAGATAGTAGTTGGAGAAGAGACAAAAACTGCAGCCCCTGAGT
  CTGAAGTTAGCAGCCCTGTTTCAAATGTTGCTATCCCAAGCACCCCACAGAGCACTGGTGTGAATACTCG
  GAGGTCCTCCCATGCTGGGGATGTAGCTGCAGAAAAGCCAATCCCTAAACCACCTCCAGCTAAGCCTTCT
  AGACCCCGACCGAAAAGTAGAATTTCTCGGTACCGGACCAGTTCAGCCCAAAGACTAAAACGCCAGAAGC
  AGGCCATTGCACAACAGGCAGAACTGTCGCAAGCTGCCTTGGAAGAAGGAGGAAGTAACAACTCAGTAAC
  TCCTCCTGAAGCTGGAAATACAGACAGTTCAGGAGAGAACAGACAGCTAACAGGGTCTGACCCAACTGTG
  ATATCAGTTACTGGATCCCATGTCAACCGTGCTGCATCTAAATACCCCAAAACCAAAAAGTATCTAGTTA
  CAGAATGGTTAAATGACAAGGCAGAGAAGCAAGAATGCCCTGTTGAGTGTCCTTTGCGTATCACCACTGA
  CCCAACTGTGTTGGCAACAACCCTGAACATGCTACCAGGTCTTATTCATTCCCCATTAATTTGTACCACC
  CCCAAACACTACATTCGCTTTGGCTCACCCTTTATGCCTGAGAGGCGTCGAAGGCCCCTTCTGCCTGATG
  GCACATTCAGCTCCTGTAAGAAGCGCTGGATAAAGCAAGCCTTGGAAGAAGGGATGACTCAGACTTCATC
  TGTGCCCCAAGAGACTAGAACACAGCACCTATACCAAAGTAATGAGACTAGTAATTCTTCTAGTATCTGT
  AAAGACAATGCAGACTTACTGAGCCCATTAAAGAAATGGAAGTCTCGCTATCTGATGGAGCAGAATATCA
  CCAAGTTGCTTCAGCCTCTGTCTCCAGTTACACCACCCCCACCCAGCTCAGGCTCAAAGAGTCCCCAGCT
  GACCACACCTGGCCAGACTCACCCAGGAGAAGAGGAGTGTCGAAATGGATACAGCCTCATGTTCTCACCA
  ATCACATCTCTTACTACTGCTAGTCGTTCCAACACTCCTCTGCAGTTTGAGCTTTGTCACCGAAAAGACC
  TAGATTTGACAAAAGTGGGATTCCCAGACTCCAGCACTCACAGCTGCGCTGATAGGCCCTCCCTGCTTAA
  CTGCAATCATCCTGACCTGGCTTCTCATCCCTCTGTTGTTCCCACCTCCGAGGCTGGCTTCCCAAGCAGG
  AGTGGAGATGGTCCTCAGACCTTGCTGAGAAACTCAGACCAGGCGTTTAGGACAGAGTTCAATTTGATGT
  ACGCCTACTCCCCTTTGAATGCTATGCCTCGAGCAGATGGACTGTATAGAGGGTCTCCCTTGGTGGGAGA
  CAGGAAGCCTTTACATTTAGATGGAGGATATTGTTCCCCTGCTGAAGGCTTTTCCAGCAGATATGAACAT
  GGCTTTATGAAAGACCTCTCTCGGGGATCCATGTCACCTGGTGGTGAAAGGACCTGTGAAGGAGTCCCAT
  CTGCTCCTCAGAACCCACCGCAAAGGAAAAAGGTATCCTTGCTAGAGTACCGCAAAAGAAAACAAGAAGC
  TAAGGAGAATTCTGGTGGGGGAAATGACTCTTCACAGAGCAAAAGCAAGTCTTCAGGAGCTGGGCAAGGC
  AGCAGTAACTCTGTTTCTGACACCGGTGCCCATGGTGTGCAGGGATCCTCAGCCGGAACCCCGTCATCCC
  CTCACAAAAAATTTTCCCCGTCTCATTCCTCTGCATCGCATTTGGAGGCGGTAAGCCCGTCAGATTCCAG
  GGGCACTTCTTCCTCTCACTGCAGACCTCAAGAAAACATCAGCAGTAGGTGGATGGTTCCTACATCAGTT
  GAACGACTCCGAGAAGGAGGTAGCATCCCTAAGGTTCTTCGAAGCAGTGTGAGAGTTGCCCAGAAGGGAG
  AGCCTTCTCCAACATGGGAGAGTAACATCACAGAGAAAGAGTCAGACCCTGCAGATGGAGAAGGCCCAGA
  GCCATTGAGCTCAGCACTCTCTAAAGGAGCAACTGTTTACAGCCCTTCCAGATATAGCTACCAGCTCCTG
  CAGTGTGACAGTCCACGGACAGAATCACAAAGCCTCCTTCAGCAGAGTTCGTCCCCCTTTAGAGGACATC
  CCACCCAATCTCCAGGATACAGTTATCGAACTACTGCACTGAGACCTGGAAACCCTCCCTCTCACGGTTC
  TTCAGAATCCTCCCTCTCTTCCACGTCCTACCCCAGCCCTGCCCACCCTGTGTCTACAGACTCGTTGGCC
  CCATTTACGGGGACTCCAGGGTATTACAGCAGCCAGCCGCATTCTGGAAACAGCACTGGCAGCAATCTTC
  CAAGGAGGAGCTGTTCTTCAAGTGCTGCTAGCCCTACCCCACAGGGCCCCTCAGACTCACCGACCTCAGA
  CTCGGTTTCTCAGTCCAGCACAGGAACTCTGAGTTCCACCTCCTTTCCTCAGAACTCTAGGTCTTCATTC
  CCATCAGACTTACGGACTATCAGTCTGCCCAATGCTGGGCAGTCAGCTGCCTACCAGGCCTCCAGGGTAT
  CTGCGGTTTCCAATTCACAGCACTACCCACATCGTGGTAGTGGGGGTGTACACCAGTACCGACTCCAGCC
  ACTGCAAGGGTCAGGAGTCAAGACTCAGACAGGACTTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211996 representing BC083184
  Red=Cloning site Green=Tags(s)
  
  MSIAIPLGVTTPDTSYSDMAAGSDPESVEASPAVNEKSVYSTHNYGTTQRHGCRGLPYADHNYGAPPPPT
  PPASPPVQTIIPRSDLNGLPSPVEERCGDSPNSEGETVPTWCPCGLSQDGFLLNCDKCRGMSRGKVIRLH
  RRKQDNISGGDSSATESWDEELSPSTVLYTATQHTPTSITLTVRRTKPKKRKKSPEKGRAAPKTKKIKNS
  PSEAQNLDENTTEGWENRIRLWTDQYEEAFTNQYSADVQNALEQHLHSNKEFVGKPAILDTINKTELACN
  NTVIGSQMQLQLGRVTRVQKHRKILRAARDLALDTLIIEYRGKVMLRQQFEVNGHFFKKPYPFVLFYSKF
  NGVEMCVDARTFGNDARFIRRSCTPNAEVRHMIADGMIHLCIYAVSAITKDAEVTIAFDYEYSNCNYKVD
  CACHKGNRNCPIQKRNPNAAELPLPPPPSFPTIGAETRRRKARRKELELEQQNEVPEENPDPQPQEVPEK
  VTVSNEHEEVDNPEEKPEEEEKEEATDDQENSAHSRRTREDRKVEAIMHAFESLEKRKKRRDQPVEQSSS
  DIEITTSSSEIVVGEETKTAAPESEVSSPVSNVAIPSTPQSTGVNTRRSSHAGDVAAEKPIPKPPPAKPS
  RPRPKSRISRYRTSSAQRLKRQKQAIAQQAELSQAALEEGGSNNSVTPPEAGNTDSSGENRQLTGSDPTV
  ISVTGSHVNRAASKYPKTKKYLVTEWLNDKAEKQECPVECPLRITTDPTVLATTLNMLPGLIHSPLICTT
  PKHYIRFGSPFMPERRRRPLLPDGTFSSCKKRWIKQALEEGMTQTSSVPQETRTQHLYQSNETSNSSSIC
  KDNADLLSPLKKWKSRYLMEQNITKLLQPLSPVTPPPPSSGSKSPQLTTPGQTHPGEEECRNGYSLMFSP
  ITSLTTASRSNTPLQFELCHRKDLDLTKVGFPDSSTHSCADRPSLLNCNHPDLASHPSVVPTSEAGFPSR
  SGDGPQTLLRNSDQAFRTEFNLMYAYSPLNAMPRADGLYRGSPLVGDRKPLHLDGGYCSPAEGFSSRYEH
  GFMKDLSRGSMSPGGERTCEGVPSAPQNPPQRKKVSLLEYRKRKQEAKENSGGGNDSSQSKSKSSGAGQG
  SSNSVSDTGAHGVQGSSAGTPSSPHKKFSPSHSSASHLEAVSPSDSRGTSSSHCRPQENISSRWMVPTSV
  ERLREGGSIPKVLRSSVRVAQKGEPSPTWESNITEKESDPADGEGPEPLSSALSKGATVYSPSRYSYQLL
  QCDSPRTESQSLLQQSSSPFRGHPTQSPGYSYRTTALRPGNPPSHGSSESSLSSTSYPSPAHPVSTDSLA
  PFTGTPGYYSSQPHSGNSTGSNLPRRSCSSSAASPTPQGPSDSPTSDSVSQSSTGTLSSTSFPQNSRSSF
  PSDLRTISLPNAGQSAAYQASRVSAVSNSQHYPHRGSGGVHQYRLQPLQGSGVKTQTGLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC083184
• ORF Size: 4380 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC083184.1
• RefSeq Size: 6372 bp
• RefSeq ORF: 4382 bp
• Locus ID: 72895
• Cytogenetics: 6 E3
• MW: 233.6 kDa
• Gene Summary: Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 in vitro (PubMed:22939622). The physiological significance of this activity is unclear (Probable) (PubMed:22939622). Probable transcriptional regulator that acts via the formation of large multiprotein complexes that modify and/or remodel the chromatin. Acts as a regulator of histone acetylation during gene transcription (PubMed:27864380).[UniProtKB/Swiss-Prot Function]