Abcc5 (NM_013790) Mouse Tagged ORF Clone

CAT#: MR211987

Abcc5 (Myc-DDK-tagged) - Mouse ATP-binding cassette, sub-family C (CFTR/MRP), member 5 (Abcc5), transcript variant 1

Product Images

Circular map for MR211987

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abcc5
• Synonyms: 2900011L11Rik; Abcc; Abcc5a; Abcc5b; AI132311; Mr; Mrp5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211987 representing NM_013790
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGATATTGACATGGGAAAAGAATATATCATCCCCAGCCCTGGGTACAGAAGTGACAGGGACAGAA
  GCGCTGTACCAGGGCAACACAGAGACCCCGAGGAACCCAGGTTCCGGAGAACAAGATCGTTGGAATGCCA
  AGATGCTCTCGAAACAGCAGCCCGAGTTGAGGGGCTTTCCCTGGATATCTCTGTGCATTCTCATCTCCAA
  ATTCTGGACGAGGAGCATTCTAAGGGAAAATACCACCATGGTTTAAGTGTCCTGAAGCCCTTCCGGACCA
  CTACCAAGCACCAGCACCCAGTGGACAATGCTGGACTTTTCTCCTACATGACCTTTTCATGGCTCTCTCC
  TCTGGCCCGAGTGGTTCACAAGAAGGGGGAGCTGTTAATGGAGGATGTGTGGCCTTTGTCCAAGTATGAG
  TCTTCTGATGTGAACAGCAGAAGACTAGAGAGACTGTGGCAAGAAGAGCTGAATGAAGTTGGGCCAGACG
  CTGCCTCCCTGCGAAGGGTTGTGTGGATCTTTTGCCGCACCAGGCTCATCCTGTCCATCGTGTGCCTGAT
  GATCACGCAGTTGGCTGGCTTCAGTGGACCAGCCTTCGTGGTGAAGCACCTCTTGGAGTACACCCAGGCA
  ACAGAGTCTAACCTGCAGTACAGCTTGCTGTTAGTGTTAGGCCTACTCCTGACAGAAGTTGTACGCTCCT
  GGTCACTTGCACTGACTTGGGCATTGAATTATCGAACTGGTGTCCGGTTGCGGGGGGCTATCCTGACTAT
  GGCATTCAAGAAGATCCTGAAGTTAAAGAACATTAAAGAAAAGTCCCTAGGGGAGCTCATCAATATCTGC
  TCCAATGATGGGCAAAGGATGTTTGAGGCAGCCGCTGTGGGCAGCTTGCTGGCTGGAGGACCTGTTGTTG
  CCATCTTGGGCATGATTTATAATGTAATCATCCTAGGACCCACGGGCTTCCTGGGATCAGCGGTTTTTAT
  CCTCTTTTATCCAGCAATGATGTTCGTGTCACGGCTAACTGCATATTTCAGGAGAAAGTGTGTAGCTGCC
  ACAGATGACCGTGTCCAGAAGATGAATGAAGTTCTTACCTACATTAAATTCATTAAAATGTATGCCTGGG
  TCAAAGCGTTTTCTCAGTGTGTGCAAAAAATCCGAGAGGAGGAACGTCGGATATTGGAGAAAGCCGGGTA
  CTTTCAGAGCATCACTGTTGGAGTGGCTCCTATTGTGGTAGTGATCGCCAGTGTGGTGACGTTCTCCGTT
  CACATGACCCTGGGCTTCCATCTGACTGCGGCACAGGCCTTCACAGTGGTGACTGTCTTCAATTCCATGA
  CTTTTGCTTTAAAAGTAACACCATTCTCAGTGAAGTCCCTCTCTGAAGCATCAGTGGCTGTTGACAGATT
  TAAGAGTTTGTTTCTAATGGAAGAGGTTCACATGATAAAGAACAAACCGGCCAGTCCTCACATCAAGATA
  GAGATGAAAAATGCCACCTTGGCATGGGACTCCTCCCACTCCAGTATACAGAACTCGCCCAAGCTGACCC
  CCAAAATGAAAAAAGACAAGAGGGCTACCAGGGGCAAGAAAGAGAAGTCGAGGCAGCTGCAACACACTGA
  GCACCAGGCGGTGCTGGCAGAACAGAAAGGACACCTCCTCCTGGACAGTGACGAGCGGCCCAGCCCGGAA
  GAGGAAGAAGGCAAGCAGATCCACACAGGGAGCCTGCGCCTGCAGAGGACACTGTACAACATTGACTTAG
  AAATTGAAGAGGGCAAACTGGTTGGAATCTGCGGCAGTGTGGGAAGTGGAAAAACCTCTCTCGTTTCAGC
  CATTTTAGGCCAGATGACGCTTTTGGAGGGCAGCATTGCCGTCAGTGGGACCTTTGCTTATGTGGCCCAA
  CAGGCCTGGATTCTCAATGCCACTCTGAGAGACAACATTCTCTTTGGGAAGGAATTTGATGAAGAGAGAT
  ACAACTCAGTGCTGAATAGCTGCTGCCTGCGGCCTGACTTGGCCATTCTCCCCAACAGCGACCTGACTGA
  GATTGGAGAGCGAGGAGCCAACCTGAGTGGTGGACAGCGCCAGAGAATCAGCCTTGCTAGAGCCTTGTAC
  AGTGATAGAAGCATCTACATCCTGGATGACCCCCTCAGTGCCTTAGATGCCCATGTGGGCAACCACATCT
  TCAACAGTGCTATCCGGAAGCGCCTCAAGTCTAAGACGGTTCTGTTTGTTACACACCAGTTACAGTATCT
  GGTCGATTGTGATGAGGTGATCTTCATGAAGGAAGGCTGTATCACAGAGAGAGGTACCCATGAGGAGCTG
  ATGAACTTAAATGGGGATTACGCTACGATTTTTAATAACCTGTTGCTGGGAGAGACACCCCCAGTTGAGA
  TTAATTCGAAAAAGGAAGCTACTGGTTCACAAAAATCACAAGACAAGGGCCCTAAGCCAGGGTCAGTGAA
  GAAGGAGAAGGCGGTGAAGTCGGAGGAAGGGCAGCTTGTGCAGGTGGAGGAGAAAGGGCAAGGTTCTGTG
  CCTTGGTCAGTCTACTGGGTCTACATCCAGGCTGCAGGGGGCCCCTTGGCTTTCCTGGTCATCATGGTCC
  TCTTCATGCTGAATGTGGGCAGCACTGCCTTCAGCACCTGGTGGCTTAGCTACTGGATCAAGCAAGGAAG
  CGGGAACAGCACAGTGTATCAAGGGAACAGAAGCTTCGTGAGTGACAGCATGAAGGACAACCCCTTCATG
  CAGTACTACGCCAGCATCTACGCCCTCTCCATGGCAGTCATGCTGATCTTGAAAGCCATTCGAGGAGTTG
  TCTTCGTCAAGGGCACACTGAGAGCCTCCTCCCGGCTCCATGATGAGCTATTCCGAAGGATCCTTAGGAG
  CCCCATGAAGTTTTTTGATACTACCCCAACAGGAAGGATTCTCAACAGGTTTTCCAAAGACATGGATGAA
  GTGGATGTGCGGCTGCCGTTCCAGGCTGAGATGTTTATTCAGAATGTAATCCTGGTGTTCTTCTGTGTTG
  GAATGATTGCTGGAGTCTTCCCATGGTTCCTCGTGGCGGTGGGGCCTCTCCTCATCCTCTTCTCACTTCT
  CCACATTGTCTCCAGGGTCCTGATTCGTGAGCTAAAGCGGTTGGACAATATCACGCAGTCTCCTTTCCTC
  TCCCACATCACGTCTAGCATTCAGGGCCTGGCCACCATCCATGCCTACAACAAAAGGCAGGAGTTTTTAC
  ACAGGTATCAGGAGCTCCTGGATGACAACCAGGCTCCCTTTTTCCTGTTCACCTGTGCAATGAGGTGGCT
  GGCAGTGCGGCTGGACCTCATCAGCATTGCCCTGATTACCACCACTGGCCTGATGATTGTTCTCATGCAT
  GGACAGATCCCTTCAGCCTATGCGGGGCTTGCCATCTCCTACGCTGTGCAGTTAACTGGACTATTCCAGT
  TCACCGTCAGACTGGCATCGGAGACAGAAGCACGGTTCACTTCCGTGGAGAGGATCAACCACTATATCAA
  GACTCTCTCTTTGGAAGCACCTGCCAGAATCAAGAACAAGGCTCCTCCCCATGACTGGCCCCAGGAGGGA
  GAAGTAACCTTTGAGAATGCAGAAATGAGATACCGGGAAAATCTCCCTCTGGTCCTTAAGAAAGTGTCCT
  TCACCATCAAGCCCAAGGAAAAGATAGGCATTGTGGGACGAACAGGGTCAGGGAAGTCCTCTTTGGGGAT
  GGCCCTCTTCCGTCTGGTGGAGCTATCTGGAGGCTGCATCAAGATTGATGGAATAAGAATCAGTGACATC
  GGCCTGGCCGACCTCCGAAGCAAACTGGCCATCATTCCTCAGGAGCCAGTGCTGTTCAGTGGCACTGTCA
  GATCAAACCTGGACCCTTTCAACCAGTACACGGAAGACCAGATCTGGGATGCTCTAGAGAGAACGCACAT
  GAAGGAATGTATTGCCCAGCTACCTCTGAAACTTGAGTCTGAAGTAATGGAGAACGGGGACAACTTCTCT
  GTTGGGGAACGGCAGCTGTTGTGCATAGCAAGAGCCCTGCTGCGTCACTGTAAGATTCTGATTTTAGATG
  AAGCTACAGCCGCTATGGACACAGAGACAGACTTACTGATCCAGGAGACCATCCGGGAAGCATTTGCGGA
  CTGCACCATGCTGACCATTGCCCATCGCCTGCACACAGTTCTGGGCTCTGACAGGATCATGGTGCTGGCC
  CAGGGACAGGTGGTGGAGTTTGACACCCCATCGGTCCTTCTGTCTAATGACAGTTCAAGATTCTATGCCA
  TGTTTGCTGCTGCAGAGAACAAGGTGGCTGTCAAGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211987 representing NM_013790
  Red=Cloning site Green=Tags(s)
  
  MKDIDMGKEYIIPSPGYRSDRDRSAVPGQHRDPEEPRFRRTRSLECQDALETAARVEGLSLDISVHSHLQ
  ILDEEHSKGKYHHGLSVLKPFRTTTKHQHPVDNAGLFSYMTFSWLSPLARVVHKKGELLMEDVWPLSKYE
  SSDVNSRRLERLWQEELNEVGPDAASLRRVVWIFCRTRLILSIVCLMITQLAGFSGPAFVVKHLLEYTQA
  TESNLQYSLLLVLGLLLTEVVRSWSLALTWALNYRTGVRLRGAILTMAFKKILKLKNIKEKSLGELINIC
  SNDGQRMFEAAAVGSLLAGGPVVAILGMIYNVIILGPTGFLGSAVFILFYPAMMFVSRLTAYFRRKCVAA
  TDDRVQKMNEVLTYIKFIKMYAWVKAFSQCVQKIREEERRILEKAGYFQSITVGVAPIVVVIASVVTFSV
  HMTLGFHLTAAQAFTVVTVFNSMTFALKVTPFSVKSLSEASVAVDRFKSLFLMEEVHMIKNKPASPHIKI
  EMKNATLAWDSSHSSIQNSPKLTPKMKKDKRATRGKKEKSRQLQHTEHQAVLAEQKGHLLLDSDERPSPE
  EEEGKQIHTGSLRLQRTLYNIDLEIEEGKLVGICGSVGSGKTSLVSAILGQMTLLEGSIAVSGTFAYVAQ
  QAWILNATLRDNILFGKEFDEERYNSVLNSCCLRPDLAILPNSDLTEIGERGANLSGGQRQRISLARALY
  SDRSIYILDDPLSALDAHVGNHIFNSAIRKRLKSKTVLFVTHQLQYLVDCDEVIFMKEGCITERGTHEEL
  MNLNGDYATIFNNLLLGETPPVEINSKKEATGSQKSQDKGPKPGSVKKEKAVKSEEGQLVQVEEKGQGSV
  PWSVYWVYIQAAGGPLAFLVIMVLFMLNVGSTAFSTWWLSYWIKQGSGNSTVYQGNRSFVSDSMKDNPFM
  QYYASIYALSMAVMLILKAIRGVVFVKGTLRASSRLHDELFRRILRSPMKFFDTTPTGRILNRFSKDMDE
  VDVRLPFQAEMFIQNVILVFFCVGMIAGVFPWFLVAVGPLLILFSLLHIVSRVLIRELKRLDNITQSPFL
  SHITSSIQGLATIHAYNKRQEFLHRYQELLDDNQAPFFLFTCAMRWLAVRLDLISIALITTTGLMIVLMH
  GQIPSAYAGLAISYAVQLTGLFQFTVRLASETEARFTSVERINHYIKTLSLEAPARIKNKAPPHDWPQEG
  EVTFENAEMRYRENLPLVLKKVSFTIKPKEKIGIVGRTGSGKSSLGMALFRLVELSGGCIKIDGIRISDI
  GLADLRSKLAIIPQEPVLFSGTVRSNLDPFNQYTEDQIWDALERTHMKECIAQLPLKLESEVMENGDNFS
  VGERQLLCIARALLRHCKILILDEATAAMDTETDLLIQETIREAFADCTMLTIAHRLHTVLGSDRIMVLA
  QGQVVEFDTPSVLLSNDSSRFYAMFAAAENKVAVKG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013790
• ORF Size: 4308 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013790.2, NP_038818.2
• RefSeq Size: 5826 bp
• RefSeq ORF: 4311 bp
• Locus ID: 27416
• UniProt ID: Q9R1X5
• Cytogenetics: 16 12.41 cM
• MW: 161.6 kDa
• Gene Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. The human protein functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that the human protein provides resistance to thiopurine anticancer drugs, 6-mercatopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl)adenine. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]