Ccdc88c (BC043115) Mouse Tagged ORF Clone

CAT#: MR211984

Ccdc88c (Myc-DDK-tagged) - Mouse coiled-coil domain containing 88C (cDNA clone MGC:58041 IMAGE:6408630)

Product Images

Circular map for MR211984

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ccdc88c
• Synonyms: Daple, mKIAA1509
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211984 representing BC043115
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGTGACGGTGTCGCAGCTCGTTGAGCTGTTTCTGCAGAGCCCTCTGGTGACCTGGGTGAAAACTT
  TTGGATCGTTTGGAAGCGGCCACCAGGACAACTTGACTCTGTACATGGATTTAGTGGACGGCATCTTTTT
  GAATCAAATCATGCTGCAAATAGACCCCAGGCCCAGCAATCAGCGCATCAACAAGCATGTCAACAATGAT
  GTCAACCTGCGCATCCAAAACCTGAGTATCCTGGTGAGAAACATCAAGACCTACTACCAGGAAGTTCTCC
  AGCAGCTGATCGTAATGAATTTGCCCAATGTCTTGATGATTGGCAAAGACCCGCTGTCTGGGAAGAGCAT
  GGAGGAGATTAAGAAGGTCCTGCTGCTGGTGCTGGGCTGTGCCGTTCAGTGTGAGAGGAAGGAGGAGTTC
  ATTGAGAGAATCAAACAGCTGGACATCGAGACCCAGGCTGGCATTGTGGCTCATATCCAGGAGGTGACCC
  ACAACCAGGAGAATGTGTTTGACCTGCAGTGGCTGGACTTGCCAGATGTGGCCCCAGAGGAGCTGGAGGC
  CCTCTCCAGAAACATGGTGTTCCACCTCCGCAGACTCATTGACGAGCGTGACGAGTGTACCGAGCTGATC
  GTGGACCTGACACAAGAGAGGGACTACCTCCAGACACAGCAGCCTCCCAGCCCCGGCAAGTTCTCCAGCC
  CAGACTCCACACCAAGCCCCACCAGCAGCCTGTCCAGTGAAGACAAGCAGCACCTAGCCGTGGAGCTGGC
  TGACACCAAGGCCAGGCTGCGGCGTGTCAGGCAGGAGCTGGAGGAGAAGACGGAGCAGCTTGCAGACACC
  AGGCACGAGGTGGATCAGCTGGTCCTGGAGCTTCAGAAGGCCAAGCAAGATAATATTCAGCTTGCAGCTG
  ATGCGCGGTCAGCCCGGGCCTATCGGGATGAGCTGGACTCGCTGAGGGAAAAGGCGAACCGTGTAGAGAG
  GCTGGAGATGGATCTGGTACGCTGCAAGGAGAAGCTGCACGACGTGGACTTCTACAAGGCTCGCATGGAG
  GAGCTGAGGGAAGACAATATCATCTTAATCGAGACCAAAGCCATGTTGGAGGAACAACTGACTGCCTCCC
  GGGCTCGCAGTGATAAAGTCCACGAGCTGGAGAAAGAGAACCTACAGCTAAAATCTAAACTCCATGACTT
  GGAACTGGATCGGGATGCAGACAAGAAACAAATTGAGAAGCTGCTGGAAGAATACATGGTTCTGGAGATG
  GCTCAGAAGCAGAGCATGAAAGAGTCGGCCCACCTTGGCTGGGAACTGGAGCAGCTGTCCAAGAACGCAG
  ACTTGTCCGACGCCTCCAGGAAATCCTTCGTCTTTGAGCTGAATGAGTGCGCCTCCAGCCGCATCCTGAA
  GCTGGAGAAGGAGAACCAAAGCCTACAGAGCACCATCCAGGGTCTGCGGGACACGTCGCTGGCGCTGGAG
  GAGAGCAGCCTCAAGTATGGGGAGCTGGAGAAGGAGAACCAGCAGCTTAGCAAGAAGATTGAAAAGTTAC
  AAACGCAGCTGGAGAGAGAAAAGCAGAGTAACCAAGACCTGGAGACGCTGAGTGAGGAGCTGATCCGAGA
  GAAGGAACAGCTACAGAGTGGCATGGAGGCCCTGAAGGCGGACAGGGCCAGGCAGATAAAGGATCTGGAA
  CAGGAGAAGGGCCACCTCCACCAGGCTGTGTGGTCCCTGCGGGAGAGGCCGCAGGTCAACAGCACGAAGG
  ATGTGGAGAAGGAGAACAGAGCCCTCCACCAGGCTGTGACAGAGGCGGGCAGCAAGCTCAGCCAGCTGGA
  GTTGGAGAAGCAGCAGCTGCACAGAGACTTGGAGGAGGCCAAGGAGAAGGGAGAGCAGGCGGAGGCGCTG
  GAGAAGGAGCTGCACCGGCTGGAGAAGGAGAATGAGCAGCTGACCAAGGAGGTGACCTCCCTGAAGGCAG
  CCACTGAGAAGGTCGAGGCCCTGGAGCATCAGAGCCAGGGTCTGGAGCTGGAGAACCGCTCGCTGAGAAA
  GTCCCTGGAGCAGGAGGTGGCCCAGCTGGAGAAGGACAAGAAACTGCTAGAGAAGGAGGCCAGACGCCTG
  TGGCAGCAGGTGGAGCTTAAGGACGCCATCCTGGATGACAGCGCTGCCAAGCTGTCGGCGGCCGAGAAGG
  AGAGCCGTGCGCTGGACAAGGAGCTGGCCCGCTGCCGGGACGTGGGCAGCAAGCTGAAGGAGCTGGAGAA
  GGACAACAGGGACCTCACCAAGCAAGTCACCATGCACACGAGGACGCTGACCACCCTGAGAGAGGACCTG
  GTGCTGGAGAAGTTGAAGAGCCAGCAGCTAAGCAGCGAGCTGGACAAGCTGAGCCAGGAGCTGGAGAAGG
  TCGGCCTCAGCAAGGACCTGTTGCTGCAGGAGGATGACGGTCACGGTGACGGAAAAGGCAAAACCGAATC
  AGCCTTGAAAACAACACTGGCTATGAAAGAAGAAAAGATTGTGTTTTTGGAGGCACAGGTGGAAGAGAAA
  GAGAGCTTGAGTCGCCAACTCCAGATTGAGCTGCAGATGATAAAGAAAGAACATGAGCAGCTGAGGCAGA
  CCCAAGAGGGTGGAGACAAGGCACAGAACGCACTGAAGCGCCCTCCGGGCAAGGTCACCAGTCACCAAGA
  GAAGGAGGCCTGGGAGCCCAGCCATAAAGAGGCCACCATGGAGCTACTCCGTGTGAAGGACCGGGCCATC
  GAGCTGGAGCGCAGTAACGCAGCTCTTCAGGCCGAGAGGCAGCTGCTGAAGGAGCAACTACAGCACTTGG
  AGACTCAGAACGTGTCCTTCAGCAGCCAGATCCTGACCCTACAGAAGCAGAGCGCCTTCCTGCAAGAGCA
  CACCACCACGCTGCAGACCCAGACTGCGAAGCTGCAGGTGGAGAATTCCACACTGAGTTCCCAGAACGCA
  GCGCTCAGTGCACAGTACACGGTGTTGCAGAGCCAGCAGGCTGCCAAGGAGGCCGAGCACGAGGGTCTGC
  AGCAGCAGCAGGAGCAGCTGGCAGCCGTCTATGAAGCCCTGCTGCAGGACCACAAGCACCTGGGCACCCT
  GTACGAGTGCCAGTCCTCAGAGTATGAGGCCCTCATCCGCCAGCACAGCTGCCTGAAGACGCTGCACAGG
  AACCTGGAGCTGGAGCACAAGGAGCTGGGGGAGAGGCACGGAGACTTACAGCAGCGTAAGGCAGAGCTGG
  AGGAGCTGGAGAAAGTACTGAGCACCGAGCGGGAGGCACTTGAGCGGGAGCAGAAGACCAACGCCATTGC
  CACGAGCGAGAACCAGAGGCTTCGAGGAGAGCTGGACAGGATCAGCTTCCTGCACCAGCAGCTGAAAGGG
  GAGTATGAAGAGCTGCACGCCCACACCAAGGAGTTGAAAACCTCGCTCAACAACTCTCAGCTGGAGCTGA
  GCCGCTGGCAGGTGCGCTTCGACGAGCTTAAGGAGCAGCACCAGAGCATGGACATCTCTCTGACCAAGAT
  GGACAACCACTGTGAGCTGCTCTCCCGGCTCAAGGGGAACTTAGAGGAGGAGAATCATCACCTGCTGAGC
  CAGATCCAGCTGCTGAGTCAGCAGAACCAGATGCTCCTGGAACAGAACATGGAGAGCAAGGAGCAGTACC
  ACGAGGAGCAGAAGCAGTACATAGACAAGTTAAACGCCTTACGGAGACACAAGGAGAAGCTGGAAGAAAA
  AATCATGGATCAGTACAAGTTCTATGACCCTGCTCCAAAGAAGAAGAACCACTGGATTGGAGCCAAAGCC
  CTAGTCAAACTTATCAAGCCAAAGAAAGAAGGTTCCAGAGAACGGTTAAAATCCACCACAGATAGCCCCC
  CCTGGCAGCTGGAGCCCTCAGACCCTGCCTCGCCATCTCCCTCTCAGGCTCTCAGATCGCAGACGGAGAA
  CCCCGACAACCCCCCATCGGGTCCAAACTGTGTGGAAGAACGAGACACCCACAACGGGCCTGTGGGGAAA
  GGCCCAGGGGATCTAAAACCAAAACGAGGGTCCCCGCGTGGAGGCAGTGTTGACCGCACAGACACCTCCA
  CTGACCCAGCTGTGAAGTCCTGGCCCTCGGAGCCTGGCTCCCGGACCTTTTCAACCTCAGCCGTGATGAC
  CTGCTTAGTGACTACTTCCGAAAGGCCCATGATCCCCCAGCCCTGGGAGGCCAGCCAGGACCACCAGCTA
  GGAAAGATGGAGCCAAGATGCCCACCAGCTTCGTGGCTCCCACCATCAAGATGTCAATCAACACCTCTGA
  GGGGCAGCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211984 representing BC043115
  Red=Cloning site Green=Tags(s)
  
  MDVTVSQLVELFLQSPLVTWVKTFGSFGSGHQDNLTLYMDLVDGIFLNQIMLQIDPRPSNQRINKHVNND
  VNLRIQNLSILVRNIKTYYQEVLQQLIVMNLPNVLMIGKDPLSGKSMEEIKKVLLLVLGCAVQCERKEEF
  IERIKQLDIETQAGIVAHIQEVTHNQENVFDLQWLDLPDVAPEELEALSRNMVFHLRRLIDERDECTELI
  VDLTQERDYLQTQQPPSPGKFSSPDSTPSPTSSLSSEDKQHLAVELADTKARLRRVRQELEEKTEQLADT
  RHEVDQLVLELQKAKQDNIQLAADARSARAYRDELDSLREKANRVERLEMDLVRCKEKLHDVDFYKARME
  ELREDNIILIETKAMLEEQLTASRARSDKVHELEKENLQLKSKLHDLELDRDADKKQIEKLLEEYMVLEM
  AQKQSMKESAHLGWELEQLSKNADLSDASRKSFVFELNECASSRILKLEKENQSLQSTIQGLRDTSLALE
  ESSLKYGELEKENQQLSKKIEKLQTQLEREKQSNQDLETLSEELIREKEQLQSGMEALKADRARQIKDLE
  QEKGHLHQAVWSLRERPQVNSTKDVEKENRALHQAVTEAGSKLSQLELEKQQLHRDLEEAKEKGEQAEAL
  EKELHRLEKENEQLTKEVTSLKAATEKVEALEHQSQGLELENRSLRKSLEQEVAQLEKDKKLLEKEARRL
  WQQVELKDAILDDSAAKLSAAEKESRALDKELARCRDVGSKLKELEKDNRDLTKQVTMHTRTLTTLREDL
  VLEKLKSQQLSSELDKLSQELEKVGLSKDLLLQEDDGHGDGKGKTESALKTTLAMKEEKIVFLEAQVEEK
  ESLSRQLQIELQMIKKEHEQLRQTQEGGDKAQNALKRPPGKVTSHQEKEAWEPSHKEATMELLRVKDRAI
  ELERSNAALQAERQLLKEQLQHLETQNVSFSSQILTLQKQSAFLQEHTTTLQTQTAKLQVENSTLSSQNA
  ALSAQYTVLQSQQAAKEAEHEGLQQQQEQLAAVYEALLQDHKHLGTLYECQSSEYEALIRQHSCLKTLHR
  NLELEHKELGERHGDLQQRKAELEELEKVLSTEREALEREQKTNAIATSENQRLRGELDRISFLHQQLKG
  EYEELHAHTKELKTSLNNSQLELSRWQVRFDELKEQHQSMDISLTKMDNHCELLSRLKGNLEEENHHLLS
  QIQLLSQQNQMLLEQNMESKEQYHEEQKQYIDKLNALRRHKEKLEEKIMDQYKFYDPAPKKKNHWIGAKA
  LVKLIKPKKEGSRERLKSTTDSPPWQLEPSDPASPSPSQALRSQTENPDNPPSGPNCVEERDTHNGPVGK
  GPGDLKPKRGSPRGGSVDRTDTSTDPAVKSWPSEPGSRTFSTSAVMTCLVTTSERPMIPQPWEASQDHQL
  GKMEPRCPPASWLPPSRCQSTPLRGSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC043115
• ORF Size: 4281 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC043115.1
• RefSeq Size: 6608 bp
• RefSeq ORF: 4283 bp
• Locus ID: 68339
• Cytogenetics: 12 E
• MW: 242.2 kDa
• Gene Summary: Negative regulator of the canonical Wnt signaling pathway, acting downstream of DVL to inhibit CTNNB1/Beta-catenin stabilization (PubMed:14750955). May also activate the JNK signaling pathway (By similarity).[UniProtKB/Swiss-Prot Function]