Smg6 (NM_001002764) Mouse Tagged ORF Clone

CAT#: MR211977

Smg6 (Myc-DDK-tagged) - Mouse Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg6)

Product Images

Circular map for MR211977

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Smg6
• Synonyms: AI317223; AU041178
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211977 representing NM_001002764
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGGGGTTGGAGCGTGTGCGGATCTCCGCATCGGAACTGCGAGGGATCCTGGCCACTCTGGCTC
  CGCAGGCCGGGAGCAGAGAAAACATGAAGGAACTAAAGGAACCCAGACAGCGCAAAGATAATAGGCGTCC
  AGATTTGGAAATCTATAAGCCTGGACTTTCTCGACTAAGGAACAGACCTAAAACTAAGGAGGCTTCTGGG
  AATGAGGAATTTAAGGATGAGATAGTAAATGACAGAGATTCTTCTGCTGTTGGGAATGATACACAGCTTA
  TTCAAGTTTGCAAGGAATTGGACAGCCAACAGCAAAATGGCCCTATAGATGCAGAAAATAGTCAGGCACA
  GGAAACTTTTCCAAAGACTGTTGGACTAGAGGACCGAAGTCTAAAGATCATCAAAAGAAGCAAGAAACCA
  GACCTGCAGATCTACCAGCCTGGAAGGCGGTTGCAGACCATTACCAAAGAGTCAGCAGGCAGGGCAGATG
  AAGAAGAGATCCTCAACCAAGTGGAGCAGTTGAGGATAGAGGAGGATGAGTGTAAGGGAGAAGCTATAAA
  AGAAGAAGTTAATAACAAACCGGATAAAACCGAGATAGAAAAGCACCAAAGTAATGACCGTGTAAGAACT
  GCCAAGGGAGAAAAGGGAAAAAAGATTGAAAAGGGGGAGGGGTCAAAGAAGGTGGCTGATGACTCTGTCC
  CAGGAAAGCCTGGCTCTGTAAAGAGATACTCGAGATCAGACAAGAGAAGGAATCGCTACCGCACTTGCAG
  TACCAGCTCAGCTGGTAGCAACAACAGTGCTGAGGGAGCTGGCCTGACCGATAATGGATGTCGCCGGCGG
  CGCCAGGATCGGGCCAAGGAGAGGCCACGACTGAAGAAGCAGGTATCTTTGTCCTCAACTGATTCCTTAG
  ATGAGGACAGAGTTGATGAGCCTGATGTCCTAGGGTCCAGGAGGAGCTCAGAAAGGAAGAAGCATTTAGA
  AAGAAATTGGTCTGGCTGTGGTGAGGGTGAACAGAAAAGCAATGGTAAAGAAAACCGAAGTGCCCTTCGT
  GTCACTTTTGATGCAGAAACCATGAGTAAAGACTCCCCTGTGGTGAGGTCAGTCAAAGATAATGTGGATA
  GAATGAAGTCTGACAAAGGCCCAAGCAGTGGGGGTAAGGGCTCTGAGAAGCAGGAGCTCAGACACCCGAG
  ACAAGAACTTCGAGATCGTGGTCGTGGTATCCTGATTCTGCCTGCCCACACTGCCCTATCTGTCAGTTCA
  TCAGGTTCTCCGGAGTCTACACCTTTGGGACCTCGACTTTTATTTGGATCTGGTAGTAAGGGATCTCGGA
  GTTGGGGCCGTGGAGGCACTACCCGCCGACTATGGGACCCAAACAATCCTGACCAGAAGCCTGCCCTAAA
  AAGCCAGACACCTCAGCTACATTTCTTGGACACTGATGATGAAATTAGCCCTACATCTTGGGGTGATTCA
  CGTCAGGCCCAAGCATCTTACTATAAATTTCAAAACTCTGATAACCCCTATTATTACCCTCGGACACCAG
  GCCCTGCCTCTCAGTATCCCTATGCAGGCTATAGCCCTCTGCAGTACCCAGTAGGCCCTACAAATGGTAT
  GTATCCAGGGGCTTACTACCCGGGCTATCCTGCCCCCTCAGGACAATATGTGTGTAGCCCTCTCCCTGCT
  AGCACCATGAGTCCTGAGGAAATAGAACAGCACGTGCGGAACATGCAGCAACAGGAGCTCCATCGGCTTC
  TCCGAGTGGCTGACAACCAGGAACTGCAGCTCAGCAACCTGCTTTCCAGGGACCGCATCAGCACTGAGGG
  CATGGAGAAGATGGCTCAGCTCAGAACTGAACTACTGCAGCTGTACGAGCGCTGTATTCTATTAGATATT
  GAATTCTCCGACAGTCAGAATGTAGATCAGATCCTGTGGAAGAACGCTTTCTATCAGGTGATTGAGAAGT
  TCAGGCAGCTTCTCAAGGATCCAAACAGTGAGAACCCAGAGCAGATTCGGAACAGACTCTTGGAGCTCTT
  AGATGAGGGCAGTGACTTCTTTGATAGTTTGCTTCAGAAACTACAGGTTACTTACAAGTTCAAACTGGAG
  GACTACATGGATGGGCTTGCCATTCGAAGCAAGCCTTTACGAAAAACAGTGAAATATGCCTTGATCAGTG
  CTCAACGAAGTATGATTTGCCAAGGAGATATCTCTAGGTATCGGGAGCAAGCCAACGATACAGCAAACTA
  TGGGAAGGCTCGCAGTTGGTATCTGAAGGCTCAGCACATTGCTCCCAAGAATGGGCGCCCCTATAACCAG
  TTGGCTCTACTGGCAGTGTATACGAGGAGGAAGCTTGATGCTGTATATTACTATATGCGCAGTTTAGCAG
  CCAGCAACCCTATCCTGACTGCCAAGGAGAGTCTCATGAGTTTATTTGAAGAGACTAAACGAAAGGCTGA
  ACAGATGGAAAAGAAGCAGCATGAGGAATTTGATATGAGCCCTGATAAGTGGCGAAAAGGAAAGAAGTCT
  ACTTTCCGGCATGTTGGGGATGACACTACTCGCCTGGAGATTTGGATTCATCCATCACATTCTCGGTCTG
  CCCAGGGCACTGAGTCTGGGAAGGATTCTGAGCAGGAGAATGGTCTGGGCAGCCTGAGTCCCAGTGATCT
  GAACAAAAGGTTCATCCTCAGTTTTCTCCATGCCCATGGGAAGCTGTTTACCCGGATTGGGATGGAGACA
  TTCCCTGCAGTGGCTGAGAAGGTCCTTAAGGAGTTCCAAGTATTGCTGCAGCATAGCCCATCTCCTATTG
  GAAGTACCCGTATGCTGCAGCTTATGACCATCAACATGTTTGCCGTGCATAACTCCCAGTTGAAAGACTG
  CTTCTCAGAGGAGTGTCGTTCTGTGATCCAGGAACAAGCTGCATCCTTGGGCTTGGCTATGTTTTCTCTA
  CTGGTCCAGCGCTGCACATGCCTACTTAAGGACTCTGCTAAAGCTCAGCTGTCCTCTCCTGAGGACCAAG
  AGGACCAAGATGACATCAAGGTATCTTCCTTCGTCCCGGACCTGAAGGAGCTGCTCCCCAGTGTGAAAGT
  CTGGTCTGATTGGATGCTTGGCTACCCAGACACCTGGAATCCTCCACCCACATCTTTGGATCTGCCCTTG
  CAGGTTGCTGTGGATGTCTGGTCAACACTAGCTGATTTCTGTAACATACTGACTGCAGTCAATCAGTCTG
  AGGTGCCACTGTACAAGGACCCAGATGATGACCTCACCCTGCTTATCCTGGAAGAGGATCGGCTTCTCTC
  TGGCTTTGTCCCCTTGCTGGCTGCCCCTCAGGACCCCTGCTACGTGGAGAAAACCTCGGATAAGGTTATT
  GCAGCTGACTGCAAAAGGGTCACAGTGCTGAAGTATTTTCTGGAAGCCCTTTGTGGACAAGAAGAGCCTC
  TGCTGGCATTCAAGGGTGGAAAATATGTGTCAGTGGCACCCGTCCCAGACACCATGGGAAAGGAAATGGG
  AAGCCAAGAGGGAAAACAACTGGAAGATGAGGAAGAAGATGTGGTGATTGAAGACTTTGAGGAAGATTCA
  GAGGCTGAAGGCAGTGGGGGTGAGGATGACATCAGGGAACTTCGGGCCAAGAAGCTGGCTCTAGCAAGGA
  AGATAGCTGAGCAGCAGCGTCGCCAGGAAAAGATCCAGGCTGTTTTGGAAGACCAGAGTCAGATGAGGCA
  GATGGAGCTGGAGATCAGACCCTTGTTCCTCGTACCAGATACCAACGGCTTCATTGACCACCTGGCCAGT
  CTGGCTCGGCTGCTGGAGAGCAGGAAGTACATCCTGGTGGTGCCCCTCATCGTGATCAATGAGCTGGACG
  GCCTCGCCAAAGGGCAGGAGACAGACCACCGGGCTGGGGGCTATGCCCGTGTGGTGCAAGAAAAGGCCCG
  AAAATCCATTGAGTTCCTCGAACGGAGATTTGAGAGTCGGGACTCCTGCCTGCGGGCCTTGACCAGCCGT
  GGCAATGAACTTGAATCTATCGCCTTCCGAAGTGAGGACATTACTGGACAGCTGGGTAACAACGATGACC
  TGATCCTCTCTTGCTGCCTGCACTACTGCAAAGACAAGGCTAAGGATTACATGCCCACCAGCAAAGAGGA
  GCCAATCCGCCTGTTACGGGAGGTGGTGTTGCTGACCGATGATCGGAACCTTCGTGTAAAGGCGCTGACA
  AGGAATGTGCCTGTGAGGGACATCCCAGCCTTCCTTACGTGGGCCCAGGTGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211977 representing NM_001002764
  Red=Cloning site Green=Tags(s)
  
  MAEGLERVRISASELRGILATLAPQAGSRENMKELKEPRQRKDNRRPDLEIYKPGLSRLRNRPKTKEASG
  NEEFKDEIVNDRDSSAVGNDTQLIQVCKELDSQQQNGPIDAENSQAQETFPKTVGLEDRSLKIIKRSKKP
  DLQIYQPGRRLQTITKESAGRADEEEILNQVEQLRIEEDECKGEAIKEEVNNKPDKTEIEKHQSNDRVRT
  AKGEKGKKIEKGEGSKKVADDSVPGKPGSVKRYSRSDKRRNRYRTCSTSSAGSNNSAEGAGLTDNGCRRR
  RQDRAKERPRLKKQVSLSSTDSLDEDRVDEPDVLGSRRSSERKKHLERNWSGCGEGEQKSNGKENRSALR
  VTFDAETMSKDSPVVRSVKDNVDRMKSDKGPSSGGKGSEKQELRHPRQELRDRGRGILILPAHTALSVSS
  SGSPESTPLGPRLLFGSGSKGSRSWGRGGTTRRLWDPNNPDQKPALKSQTPQLHFLDTDDEISPTSWGDS
  RQAQASYYKFQNSDNPYYYPRTPGPASQYPYAGYSPLQYPVGPTNGMYPGAYYPGYPAPSGQYVCSPLPA
  STMSPEEIEQHVRNMQQQELHRLLRVADNQELQLSNLLSRDRISTEGMEKMAQLRTELLQLYERCILLDI
  EFSDSQNVDQILWKNAFYQVIEKFRQLLKDPNSENPEQIRNRLLELLDEGSDFFDSLLQKLQVTYKFKLE
  DYMDGLAIRSKPLRKTVKYALISAQRSMICQGDISRYREQANDTANYGKARSWYLKAQHIAPKNGRPYNQ
  LALLAVYTRRKLDAVYYYMRSLAASNPILTAKESLMSLFEETKRKAEQMEKKQHEEFDMSPDKWRKGKKS
  TFRHVGDDTTRLEIWIHPSHSRSAQGTESGKDSEQENGLGSLSPSDLNKRFILSFLHAHGKLFTRIGMET
  FPAVAEKVLKEFQVLLQHSPSPIGSTRMLQLMTINMFAVHNSQLKDCFSEECRSVIQEQAASLGLAMFSL
  LVQRCTCLLKDSAKAQLSSPEDQEDQDDIKVSSFVPDLKELLPSVKVWSDWMLGYPDTWNPPPTSLDLPL
  QVAVDVWSTLADFCNILTAVNQSEVPLYKDPDDDLTLLILEEDRLLSGFVPLLAAPQDPCYVEKTSDKVI
  AADCKRVTVLKYFLEALCGQEEPLLAFKGGKYVSVAPVPDTMGKEMGSQEGKQLEDEEEDVVIEDFEEDS
  EAEGSGGEDDIRELRAKKLALARKIAEQQRRQEKIQAVLEDQSQMRQMELEIRPLFLVPDTNGFIDHLAS
  LARLLESRKYILVVPLIVINELDGLAKGQETDHRAGGYARVVQEKARKSIEFLERRFESRDSCLRALTSR
  GNELESIAFRSEDITGQLGNNDDLILSCCLHYCKDKAKDYMPTSKEEPIRLLREVVLLTDDRNLRVKALT
  RNVPVRDIPAFLTWAQVG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001002764
• ORF Size: 4254 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002764.2
• RefSeq Size: 5817 bp
• RefSeq ORF: 4257 bp
• Locus ID: 103677
• UniProt ID: P61406
• Cytogenetics: 11 B5
• MW: 160.5 kDa
• Gene Summary: Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. May have a general role in telomere regulation. Promotes in vitro the ability of TERT to elongate telomeres. Overexpression induces telomere uncapping, chromosomal end-to-end fusions (telomeric DNA persists at the fusion points) and did not perturb TRF2 telomeric localization. Binds to the single-stranded 5'-(GTGTGG)(4)GTGT-3' telomeric DNA, but not to a telomerase RNA template component (TER).[UniProtKB/Swiss-Prot Function]