Il16 (NM_010551) Mouse Tagged ORF Clone

CAT#: MR211924

Il16 (Myc-DDK-tagged) - Mouse interleukin 16 (Il16)

Product Images

Circular map for MR211924

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Il16
• Synonyms: mKIAA4048
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211924 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCCCACGGCCACTCCGGAAAGAGCCGGAAATCCACGAAATTTCGCTCCATCTCCCGTTCCCTGA
  TCCTCTGTAACGCCAAGACCAGTGATGACGGCTCCAGCCCAGATGAGAAGTACCCTGACCCTTTTGAGAC
  TTCACTGTGCCAGGGCAAGGAGGGTTTCTTTCACTCCTCCATGCAGCTGGCAGACACATTTGAGGCTGGG
  CTCAGCAATATCCCAGACCTCGCACTGGCCTCGGACTCTGCTCAACTCGCAGCAGCTGGGAGCGATCGAG
  GCAAGCACTGCAGGAAGATGTTCTTCATGAAGGAATCTTCTTCAACTTCCTCTAAAGAAAAGTCTGGAAA
  ACCAGAAGCACAAAGTAGCAGCTTCCTGTTTCCTAAAGCTTGCCACCAAAGGACACGCAGCAACTCCACC
  AGTGTTAACCCCTACAGTGCTGGAGAAATCGATTTTCCAATGACCAAGAAATCTGCAGCTCCCACCGACC
  GGCAGCCCTACTCCCTCTGTAGCAACAGGAAGTCCCTCTCTCAACAACTGGACTACCCAATTTTGGGCAC
  TGCAAGACCAACTCGGTCACTGAGCACAGCTCAGCTCGGGCAGCTGTCTGGGGGTCTGCAGGCGTCAGTC
  ATCTCCAACATAGTGCTGATGAAGGGCCAAGCAAAGGGCCTGGGCTTCAGCATTGTTGGAGGAAAGGACA
  GCATTTACGGCCCTATTGGAATTTATGTGAAGAGCATCTTTGCAGGGGGAGCAGCTGCAGCTGATGGACG
  GCTTCAGGAAGGTGATGAAATTCTGGAACTCAATGGTGAATCAATGGCTGGCCTAACACACCAGGATGCT
  TTGCAGAAGTTTAAGCAAGCCAAGAAGGGGCTGCTGACTCTGACAGTGAGGACTCGCCTGACAACACCCC
  CTTCACTATGCAGCCACCTGTCACCTCCTCTGTGCCGTTCCCTGAGCTCCAGCACATGTGGTGCCCAGGA
  CAGCAGCCCCTTCTCCTTGGAAAGTCCAGCGTCTCCTGCTAGCACTGCCAAACCCAACTACAGAATCATG
  GTGGAGGTGTCTCTGAAGAAAGAGGCAGGTGTTGGCCTGGGGATCGGCCTGTGCAGCATCCCCTACTTCC
  AGTGCATCTCAGGCATCTTCGTTCACACACTGTCACCAGGATCCGTGGCACATCTTGATGGACGGCTGAG
  GTGTGGTGATGAGATTGTGGAAATCAATGATTCTCCAGTGCACTGCCTGACTCTCAATGAAGTCTACACA
  ATCCTGAGCCACTGTGACCCTGGCCCAGTTCCCATCATCGTAAGCCGACATCCGGACCCACAGGTTTCTG
  AACAGCAACTCAAGGAAGCTGTGGCTCAGGCTGTGGAAGGCGTCAAGTTTGGGAAGGACAGGCACCAGTG
  GAGCTTGGAAGGTGTCAAAAGGCTGGAGAGCAGCTGGCATGGGCGGCCTACCTTGGAGAAAGAACGTGAG
  AAGCACTCAGCGCCCCCACATCGAAGGGCCCAGAAGATCATGGTCCGATCCAGCAGTGACAGCAGCTACA
  TGTCTGGGTCACCAGGGGGAAGTCCCTGCAGTGCAGGTGCGGAGCCACAGCCTTCTGAGAGGGAAGGCAG
  CACACACAGTCCCAGCCTGTCCCCAGGGGAGGAGCAAGAGCCGTGCCCAGGGGTCCCATCCAGGCCTCAG
  CAGGAGAGCCCACCCCTTCCTGAGAGCTTGGAAAGAGAGAGCCACCCACCACTGAGACTGAAGAAATCTT
  TTGAGATTTTGGTGAGAAAGCCGACATCCTCCAAACCCAAGCCTCCACCCAGAAAATACTTTAAAAATGA
  CAGCGAGCCTCAGAAGAAACTGGAGGAGAAAGAGAAGGTCACAGACCCTTCTGGGCACACCTTGCCCACC
  TGCAGCCAGGAAACCAGAGAGCTGCTGCCACTGTTGCTACAGGAAGACACTGCAGGGAGAGCCCCCTGCA
  CCGCTGCCTGCTGCCCAGGACCTGCAGCCAGCACGCAGACTTCATCCTCCACAGAAGGAGAGTCAAGGAG
  GAGTGCCAGCCCAGAGACTCCAGCATCCCCAGGAAAACACCCGCTGCTGAAGAGGCAGGCACGGATGGAC
  TATAGCTTTGATATCACTGCTGAAGACCCTTGGGTTAGAATTTCCGACTGCATCAAAAACTTATTTAGCC
  CCATCATGAGTGAGAACCATAGCCATACACCACTACAGCCCAACACCAGCCTTGGTGAAGAGGATGGGAC
  ACAGGGCTGTCCAGAGGGGGGCCTGTCAAAAATGGACGCTGCCAATGGTGCTCCCAGAGTTTACAAGTCA
  GCAGATGGCAGCACTGTGAAGAAGGGGCCTCCAGTAGCCCCAAAGCCAGCTTGGTTTCGGCAGAGCTTGA
  AAGGTCTGAGGAATCGTGCCCCAGACCCCAGAAGACCCCCGGAAGTAGCCTCAGCCATTCAGCCTACACC
  AGTTTCTAGGGACCCACCAGGGCCACAGCCTCAGGCCTCTTCCTCCATCAGGCAGAGAATCAGCTCCTTT
  GAAAACTTTGGCTCCTCACAATTGCCAGACAGAGGAGTCCAGAGACTGAGCCTGCAGCCCAGCTCTGGGG
  AGACCACCAAATTTCCAGGAAAGCAAGATGGAGGACGATTTTCTGGTCTCCTGGGTCAAGGGGCTACAGT
  AACAGCTAAGCACAGACAGACAGAGGTAGAGTCTATGTCCACAACCTTCCCTAACTCCTCAGAGGTCAGG
  GACCCAGGTTTGCCTGAGTCTCCTCCCCCAGGTCAGCGACCCAGCACAAAAGCTTTGTCTCCAGACCCTC
  TCTTGAGACTGTTGACTACACAATCCGAGGATACTCAAGGACCAGGTCTCAAGATGCCAAGTCAGCGGGC
  ACGGAGCTTCCCCCTGACCAGGACCCAGTCCTGCGAGACAAAGCTGTTGGATGAAAAGGCCAGTAAGCTT
  TACTCCATCAGCAGCCAGCTATCATCTGCTGTCATGAAATCCCTGCTGTGCCTTCCATCTTCAGTCTCTT
  GTGGCCAGATCACCTGCATCCCCAAGGAGAGGGTGTCTCCAAAGTCACCTTGCAACAACAGCTCAGCTGC
  AGAGGGTTTTGGTGAAGCCATGGCCTCTGACACAGGGTTCTCTCTCAACCTTTCAGAGCTGAGAGAATAT
  TCAGAAGGTCTCACAGAGCCCGGGGAAACCGAGGACAGGAACCACTGTTCTTCTCAGGCCGGCCAGTCGG
  TTATCTCCTTGCTGAGTGCAGAAGAACTGGAAAAACTCATTGAGGAAGTGAGAGTTCTAGATGAAGCAAC
  ATTGAAGCAATTAGACAGCATTCATGTCACCATCTTACACAAGGAAGAAGGTGCTGGTCTTGGATTCAGC
  TTGGCAGGAGGGGCAGATCTAGAAAATAAGGTGATCACGGTTCACAGAGTGTTTCCAAATGGGCTGGCTT
  CCCAGGAAGGCACAATTCAGAAAGGCAACGAGGTTCTTTCCATCAATGGCAAATCTCTCAAGGGCGCTAC
  CCACAATGATGCCCTAGCTATCCTTCGCCAAGCTCGGGACCCAAGGCAAGCTGTGATTGTCACCAGGAGG
  ACAACTGTGGAGGCCACTCATGACCTGAACTCCTCTACTGACTCCGCAGCCTCAGCTTCAGCAGCCAGTG
  ACATTTCTGTAGAATCTAAGGAGGCCACTGTCTGCACAGTGACACTGGAGAAGACCTCTGCAGGGCTGGG
  CTTCAGCCTGGAGGGAGGAAAGGGCTCCCTGCATGGTGACAAGCCTCTTACCATAAACAGGATTTTCAAA
  GGGACAGAACAGGGTGAGATGGTGCAGCCAGGGGATGAGATCTTGCAGCTCGCTGGCACTGCTGTGCAAG
  GTCTTACCCGGTTTGAAGCCTGGAATGTCATCAAGGCATTGCCTGATGGACCTGTCACTATAGTCATCAG
  AAGGACCAGCCTCCAGTGCAAGCAGACAACAGCTTCTGCAGACTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211924 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPHGHSGKSRKSTKFRSISRSLILCNAKTSDDGSSPDEKYPDPFETSLCQGKEGFFHSSMQLADTFEAG
  LSNIPDLALASDSAQLAAAGSDRGKHCRKMFFMKESSSTSSKEKSGKPEAQSSSFLFPKACHQRTRSNST
  SVNPYSAGEIDFPMTKKSAAPTDRQPYSLCSNRKSLSQQLDYPILGTARPTRSLSTAQLGQLSGGLQASV
  ISNIVLMKGQAKGLGFSIVGGKDSIYGPIGIYVKSIFAGGAAAADGRLQEGDEILELNGESMAGLTHQDA
  LQKFKQAKKGLLTLTVRTRLTTPPSLCSHLSPPLCRSLSSSTCGAQDSSPFSLESPASPASTAKPNYRIM
  VEVSLKKEAGVGLGIGLCSIPYFQCISGIFVHTLSPGSVAHLDGRLRCGDEIVEINDSPVHCLTLNEVYT
  ILSHCDPGPVPIIVSRHPDPQVSEQQLKEAVAQAVEGVKFGKDRHQWSLEGVKRLESSWHGRPTLEKERE
  KHSAPPHRRAQKIMVRSSSDSSYMSGSPGGSPCSAGAEPQPSEREGSTHSPSLSPGEEQEPCPGVPSRPQ
  QESPPLPESLERESHPPLRLKKSFEILVRKPTSSKPKPPPRKYFKNDSEPQKKLEEKEKVTDPSGHTLPT
  CSQETRELLPLLLQEDTAGRAPCTAACCPGPAASTQTSSSTEGESRRSASPETPASPGKHPLLKRQARMD
  YSFDITAEDPWVRISDCIKNLFSPIMSENHSHTPLQPNTSLGEEDGTQGCPEGGLSKMDAANGAPRVYKS
  ADGSTVKKGPPVAPKPAWFRQSLKGLRNRAPDPRRPPEVASAIQPTPVSRDPPGPQPQASSSIRQRISSF
  ENFGSSQLPDRGVQRLSLQPSSGETTKFPGKQDGGRFSGLLGQGATVTAKHRQTEVESMSTTFPNSSEVR
  DPGLPESPPPGQRPSTKALSPDPLLRLLTTQSEDTQGPGLKMPSQRARSFPLTRTQSCETKLLDEKASKL
  YSISSQLSSAVMKSLLCLPSSVSCGQITCIPKERVSPKSPCNNSSAAEGFGEAMASDTGFSLNLSELREY
  SEGLTEPGETEDRNHCSSQAGQSVISLLSAEELEKLIEEVRVLDEATLKQLDSIHVTILHKEEGAGLGFS
  LAGGADLENKVITVHRVFPNGLASQEGTIQKGNEVLSINGKSLKGATHNDALAILRQARDPRQAVIVTRR
  TTVEATHDLNSSTDSAASASAASDISVESKEATVCTVTLEKTSAGLGFSLEGGKGSLHGDKPLTINRIFK
  GTEQGEMVQPGDEILQLAGTAVQGLTRFEAWNVIKALPDGPVTIVIRRTSLQCKQTTASADS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010551
• ORF Size: 3969 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010551.2, NM_010551.3, NP_034681.2
• RefSeq Size: 4989 bp
• RefSeq ORF: 3969 bp
• Locus ID: 16170
• UniProt ID: O54824
• Cytogenetics: 7 47.43 cM
• MW: 141.4 kDa
• Gene Summary: Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.[UniProtKB/Swiss-Prot Function]