Sec24b (NM_207209) Mouse Tagged ORF Clone

CAT#: MR211855

Sec24b (Myc-DDK-tagged) - Mouse Sec24 related gene family, member B (S. cerevisiae) (Sec24b)

Product Images

Circular map for MR211855

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sec24b
• Synonyms: AI605202; SEC24
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211855 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCCCCCGCCGGGTCCCCTCACCCGGCCGCCGGCGCCCGGATGCCGCCCAAGCTCGGCGGAGCTG
  TCTCCGGCCTGGCACCGCCGCAGCAGAACGGTCCAGCACAGAGTCCAATGCAAGTTCCATCTGGGTATGG
  GCTGCCTCATCAAAACTATATGGTCCCTTCAGGACATTACTCTCAAGGACCTGGGAAAATGACCTCCTTG
  CCATTGGATAGCCAGTGTGATAGTTACTACTCTCGTCCATATACAGTACCAACACAAAATTCGGGGACTC
  CCAGCTCAGCAAACCAACCAGGAGCACAGCAGATGTATGGCAGAGGTCCTTCCGCTCCTCACATGGGGGG
  CTCCATGCCCGGATCTTTCCAAGGAATTCCAGCATCAGCATCCCATTCCTATTCGAGTGCCTCCCAGCCC
  TACTCCTCTCTTGGGAATCGCTACAGTAGTCCTACCACGTACTCTGCCAATGCTTCTGTTGCTTCTCAGG
  GATATCCCTCTACCTGTAGTCATTATCCCATCTCAACTGTGTCTAATGTGGTGTATCCTAATGTTTCCTA
  TCCCTCGCTGCCTGCCAGTGAGCCATATGGGCAGATGTTTACCTCACAGAGTGCTCCTCCACCTGCCAGG
  CCAGTTAAAGAGTCATACTCTGGCCCCAGCACAGCTCTTACCTACCCCTCGAGACCTCCACCTCCACCTT
  CTCAGCACCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGAGTCATTCAGGATATAG
  TTCTCTACCATGGTCAGGTCCAGCCCTTCCACCAGCCCAAGACAGCCTCATCCGAAACCAAATGGGATCC
  TTGGCTACAGCGAACAGCCACCCAACTAATAATGAAAATGTCCAACCTCCCAAGTCAAGCTCCGTAGTAT
  CCACAGTTTTGCCAGGACCCTCATCAACAAGAATGCCACCTGCTCCAAGTCACCCAGTTGGGCCTGTGCC
  CTCCGCTCCACCCCCCCCAGAGCAGATGCAGACTAAAGGCATGCAGTATGGTGACTATGGTAATAATCAA
  GCTAGCTCCACAGCGACTCCCTTGTCATCAGCTTCCGATGATGAGGAGGAGCAGGAGGAGGATGAGGAAG
  CAGGTGTTGACAGCTCTTCCACCACCAGCAGTGCTTCGCCCTTGCCCAACAGTTACGATGCCTTGGAAGG
  AGGCAGCTACCCAGATATGCATTCTTCTTCAGCAAGCAGCCCTGTTCCTGACCATGCCCTGGAACCCAGT
  CCCACCCTCGCCCAGGCTCTGTCTGCAGCCCCCACTCCCCCTGCAGCTCAGCCAGCCAAGGTGGCAAAGC
  CTTTTGGCTATGGCTACCCAGCTCTGCAGCCTGCCTATCAGAACGCAGCACCACCACCCATGCCCGCAGC
  ACATCCCAGCGGCCCAGCGTACACTGGATATCCTCAGCATTACCCAGGTGTGAACCAGCTGTCCTCAGGT
  CTCGGAGGACTAAGCCTGCAGAGTTCTCCACAGCCAGAAAGCCTAAGACCTGTCAACCTCACCCAGGAGA
  AAAATATTTTACCTCCAACTCCTATTTGGGCTCCTGTACCTAACCTGAGTGCAGAACTCAGCAAGTTAAA
  CTGTAGTCCAGACTCGTTTCGATGTACCTTGACCAGTATCCCCCAGACACAGGCTTTACTGAATAAAGCT
  AAGCTTCCTTTAGGATTGCTGTTGCATCCCTTCAGAGACCTGACGCAATTGCCAGTGATAACCTCAAACA
  CCATCGTGAGGTGCCGGTCCTGTCGGACGTACATCAACCCTTTTGTGTCCTTCATTGATCAGCGAAGATG
  GAAATGCAACCTGTGTTATAGAGTTAATGATGTTCCAGAAGAGTTTTTGTACAACCCCCTGACTCGGTCT
  TACGGAGAGCCTCATAAACGGCCAGAAGTCCAGAATTCTACTGTGGAGTTCATCGCATCCTCAGACTACA
  TGCTCCGGCCTCCTCAGCCCGCGGTCTACTTGTTTGTTTTAGATGTGTCTCATAACGCAGTGGAAGCTGG
  GTATTTGACAGTTTTATGCCAGTCCTTATTAGAAAACTTAGACAAGCTTCCTGGAGATTCACGAACAAGA
  ATAGGATTCATGACTTTTGACAGCACCATTCACTTCTACAATCTACAAGAAGGGTTATCCCAGCCGCAGA
  TGTTGATTGTATCTGATATAGACGATGTTTTTCTGCCCACGCCGGATAGTCTGCTTGTGAATCTGTATGA
  AAGCAAAGAGCTTATAAAAGACTTACTGAATGCATTACCAAGTATGTTCATCAATACCAGAGAAACACAC
  AGTGCCCTTGGACCTGCACTTCAGGCTGCCTTCAAACTCATGTCTCCCACGGGCGGCCGCGTGTCTGTAT
  TTCAGACACAGTTACCTTCCTTGGGGGCAGGACTTCTGCAGTCCAGAGAAGATCCGAATCAGAGGTCGAG
  TACCAAGGTGGTGCACCATCTTGGCCCTGCAACCGACTTTTATAAGAAGCTCGCTTTAGATTGCTCAGGG
  CAGCAAACTGCAGTGGATTTGTTCCTTCTAAGTTCACAGTATTCTGACCTTGCTTCTCTGGCTTGCATGT
  CCAAGTACTCTGCAGGGTGCATATTCTACTATCCATCTTTCCACTCTACCCACAATCCTTCGCAAGCAGA
  AAAGTTACAAAAAGACCTAAAACGGTACCTCACGAGGAAGATTGGATTTGAAGCTGTTATGAGAATAAGA
  TGCACTAAAGGGCTCTCAATGCACACTTTCCACGGGAACTTCTTTGTCCGCTCCACCGACTTACTGTCTC
  TTGCCAACATCAATCCTGATGCTGGGTTTGCAGTACAGTTGTCAATCGAAGAGAGCCTGACAGATACTGC
  CTTAGTGTGCTTTCAGACAGCCCTGCTGTACACATCTAGCAAAGGCGAGCGGAGAATTCGAGTGCATACA
  CTGTGCTTGCCAGTCGTAAGTTCCCTCGCTGACGTTTATGCAGGAGTGGATGTGCAAGCCGCTGTCTGCC
  TTCTCGCCAACATGGCTGTGGACCGCTCAGTGTCATCGAGCCTGTCGGATGCCAGGGATGCCCTGGTGAA
  CGCTGTGGTGGATCCCCTGTCTGCCTACAGCTCCGCCGTGGCAAGTGTGCCCCGCTCCACCCTGACAGCA
  CCAAGCTCACTTAAGCTGCTCCCACTCTATGTTTTGGCACTTCTTAAACAGAAAGCATTTAGAACAGGGA
  CAAGCACACGCCTGGATGACCGTGTCTATGCCATGTGCCAGATGAAGTCGCAGCCGCTTGTGCATCTCAT
  GAAAATGATTCATCCCAACTTATACAGGATAGACAGGCTGACAGACGAGGGCGCCATCCACGTGAATGAC
  AGGGTAGTACCTCAGCCACCGCTTCAGAAGCTGTCTGCAGAGAAGCTGACCCGAGAAGGCGCTTTCCTCA
  TGGACTGCGGCTCAGTTTTCTACATTTGGGTTGGCAAAGGCTGTGACAGTAACTTCATAGAGAACGTGCT
  TGGATACCCTGACTTTGCATCAATACCACAGAAAATGACACATCTTCCAGAGCTAGACACACTTCCATCA
  GAAAGAACTAGATCCTTTGTGACTTGGCTCAGGGACAGCCGACCGTTAAGCCCTGTCCTTCACTTGGTAA
  AAGATGAGAGCCCTGCCAAAACAGACTTTTTCCAGCATTTGCTTGAAGACCGCACGGAAGCCGCACTCTC
  CTACTATGAGTTTCTGATTCACATCCAACAGCAGGTTTGTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211855 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSAPAGSPHPAAGARMPPKLGGAVSGLAPPQQNGPAQSPMQVPSGYGLPHQNYMVPSGHYSQGPGKMTSL
  PLDSQCDSYYSRPYTVPTQNSGTPSSANQPGAQQMYGRGPSAPHMGGSMPGSFQGIPASASHSYSSASQP
  YSSLGNRYSSPTTYSANASVASQGYPSTCSHYPISTVSNVVYPNVSYPSLPASEPYGQMFTSQSAPPPAR
  PVKESYSGPSTALTYPSRPPPPPSQHQQQQQQQQQQQQQQQSHSGYSSLPWSGPALPPAQDSLIRNQMGS
  LATANSHPTNNENVQPPKSSSVVSTVLPGPSSTRMPPAPSHPVGPVPSAPPPPEQMQTKGMQYGDYGNNQ
  ASSTATPLSSASDDEEEQEEDEEAGVDSSSTTSSASPLPNSYDALEGGSYPDMHSSSASSPVPDHALEPS
  PTLAQALSAAPTPPAAQPAKVAKPFGYGYPALQPAYQNAAPPPMPAAHPSGPAYTGYPQHYPGVNQLSSG
  LGGLSLQSSPQPESLRPVNLTQEKNILPPTPIWAPVPNLSAELSKLNCSPDSFRCTLTSIPQTQALLNKA
  KLPLGLLLHPFRDLTQLPVITSNTIVRCRSCRTYINPFVSFIDQRRWKCNLCYRVNDVPEEFLYNPLTRS
  YGEPHKRPEVQNSTVEFIASSDYMLRPPQPAVYLFVLDVSHNAVEAGYLTVLCQSLLENLDKLPGDSRTR
  IGFMTFDSTIHFYNLQEGLSQPQMLIVSDIDDVFLPTPDSLLVNLYESKELIKDLLNALPSMFINTRETH
  SALGPALQAAFKLMSPTGGRVSVFQTQLPSLGAGLLQSREDPNQRSSTKVVHHLGPATDFYKKLALDCSG
  QQTAVDLFLLSSQYSDLASLACMSKYSAGCIFYYPSFHSTHNPSQAEKLQKDLKRYLTRKIGFEAVMRIR
  CTKGLSMHTFHGNFFVRSTDLLSLANINPDAGFAVQLSIEESLTDTALVCFQTALLYTSSKGERRIRVHT
  LCLPVVSSLADVYAGVDVQAAVCLLANMAVDRSVSSSLSDARDALVNAVVDPLSAYSSAVASVPRSTLTA
  PSSLKLLPLYVLALLKQKAFRTGTSTRLDDRVYAMCQMKSQPLVHLMKMIHPNLYRIDRLTDEGAIHVND
  RVVPQPPLQKLSAEKLTREGAFLMDCGSVFYIWVGKGCDSNFIENVLGYPDFASIPQKMTHLPELDTLPS
  ERTRSFVTWLRDSRPLSPVLHLVKDESPAKTDFFQHLLEDRTEAALSYYEFLIHIQQQVCK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_207209
• ORF Size: 3756 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207209.1
• RefSeq Size: 4702 bp
• RefSeq ORF: 3756 bp
• Locus ID: 99683
• Cytogenetics: 3 G3
• MW: 135.6 kDa