Brip1 (NM_178309) Mouse Tagged ORF Clone

CAT#: MR211750

Brip1 (Myc-DDK-tagged) - Mouse BRCA1 interacting protein C-terminal helicase 1 (Brip1)

Product Images

Circular map for MR211750

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Brip1
• Synonyms: 3110009N10Rik; 8030460J03Rik; BACH; Bach1; FACJ; Fancj; OF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211750 representing NM_178309
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCAGTGTTGTCTGACTACACCATCGGTGGGGTGAAGATCCACTTTCCCTGTAGAGCGTATCCAG
  CACAGCTTGCGATGATGAATTCTATTGTCAGAGGACTAAACAGCAGCCAACACTGTTTGCTGGAGAGTCC
  CACAGGAAGTGGAAAAAGTTTAGCCTTACTTTGTTCTGCATTAGCATGGCAGCAATCTCTTAGTGAGAAG
  CCAGTGGATGAGGGCCTGAATAAAAAGCCTGAGGCACCACCTTCATGTTCTTGTGCATGCCATTCAAAGA
  ATTTTACCTACAGCGACACAAACCTGGACACTTCACCTCATTTCAATAGTCCAAGCAAACCATCTTCTGG
  AAGAAATGGAGTCTCAACTCCCTGTCAAGACTCTCCTGAAAAAAATACTCTGGCTGCAAAGTTATCTGCC
  AAGAAACAGGCATCTATACACAGAGACGAAGATGATGATTTTCAGGTAGAGAAGAAAAGAATCCGACCCT
  TAGAAACTACACAGCAGATAAGAAAACGTCATTGTTTGGAAAAGGACGTCCACCATGTAGATGCACGACT
  AGCTTCGGAAAAGAGAGTAAAGCCTGAGTCTCCGATAGGAAAGTCTTTCTCCGACAGGAAAGACTCTTTT
  CAAAATGTGGATGGCCTTTGTTCCAGGTGCTGCTGTTCTGCTAAACAAGGAAACAACCAAGAGCCTGCAA
  ATACTGTTAAGAAAGATCACGGGGGACAATGCAAGAGACCTAAAATTTATTTTGGGACGCGGACACACAA
  GCAGATTGCTCAGATTACCAGGGAGCTCCGTAAGACAGCTTACTCAGGGGTCCCGATGACTATTCTTTCC
  AGCAGGGACCACAGCTGTGTCCATCCTGAAGTGGTGGGGAATTTCAACAGAAAGGAGAAGTGCATGGAGC
  TGCTGGATGGCAAGCACGGGAAGTCCTGCTATTTCTATCATGGTGTTCATAAAATTAGTAATCAGCAGAC
  ACTGCAGCACCTTCAGGGCATGTCCAGAGCCTGGGACATAGAAGAACTAGTCAGCCTCGGGAGGAAACTG
  AAAGCTTGTCCCTACTATACAGCTCGAGAACTGATAGAGGATGCTGACATAGTGTTTTGTCCCTACAACT
  ACCTTTTAGACTCACAAATAAGGGAAACTATGGATATAAAGCTGAAAGGACAAGTTGTCATTTTAGATGA
  AGCTCACAACATTGAAGACTGTGCTCGGGAATCAGCAAGCTACAGTGTAACCGAAGTTCAGCTTAGGTTT
  GCTCGGGATGAGCTAGATAGTTTGATCAACGGTAACATAAGGAAGAAAAGCCACGAGCCCCTACGAGATG
  TGTGCTATAACCTCATCAATTGGTTAGAAACAAATTCTAAGCATCTTGTAGAAAGGGGTTATGAGTCGTC
  TTGTAAAATATGGAGTGGAAATGAGATGCTCTTAAACTTATACAGGATGGGCATCACCACGGCTACTTTT
  CCAGTTTTGCAGAGGCATCTCTCAGCTGTTCTTCAAAAAGAAGAGAAAGTCACACCAATCCATGGTAAAG
  AGGAGGCAATACAAATCCCTATTATCAGTGCATCAACTCAAGTCGTGCTCAAAGGACTCTTTATGGTGCT
  TGACTATCTCTTTAGAGAAAATAGCAGATTTGCAGATGATTATAAAGTTGCTATTCAACAGACTTACTCC
  TGGACAAATCAGATTGCTATTTTTGATAAAACTGGAGTCTTGGCTGTACCAAAAAATAAGAAACATTCAC
  GACAGAAAATTGGAGTTAATGCGCTAAACTTTTGGTGTTTAAATCCAGCTGTGGCCTTTTCAGATATTAA
  TGACAAAGTACGGACCATTGTTCTAACATCAGGAACACTCTCACCCCTGAAATCCTTTTCATCTGAGCTT
  GGTGTCACATTTAGCATCCAACTGGAGGCTAACCATGTCATCAGTAACTCACAGGTTTGGGTTGGTACTG
  TTGGCTCAGGCCCTAAGGGTCGGAATCTCTGCGCTACCTTTCAGCATACTGAAACATTTGAGTTCCAGGA
  TGAAGTGGGAATGCTTCTGTTATCTGTGTGCCAGACTGTGAGCCAAGGAATTCTGTGCTTTTTGCCATCT
  TACAAGCTATTAGAGAAATTAAGAGAACGTTGGATTTTCACTGGCCTATGGCATAGTTTGGAGTCAGTGA
  AGACAGTCATTGCAGAACCCCAAGGAGGAGAAAAAACTGATTTTGATGAATTGCTGCAAGTGTACTATGA
  CGCCATCAAGTTCAAAGGAGAAAAGGATGGAGCCCTTCTCATAGCAGTCTGTCGTGGCAAGGTGAGCGAG
  GGTCTGGATTTCTCAGATGATAACGCCCGTGCTGTCATAACCGTAGGGATTCCTTTCCCAAATGTGAAGG
  ATCTTCAGGTGGAGCTAAAACGACAATACAATGACCACCACTCAAAATCGAGAGGTCTTCTGCCTGGCCG
  TCAGTGGTATGAAATTCAAGCATACCGAGCCTTGAATCAGGCTCTAGGCAGATGTATTCGGCACAAAAAT
  GATTGGGGAGCTCTTATTCTAGTGGACGATCGCTTCAATAACAATCCAAACCGCTATATATCAGGACTTT
  CTAAATGGGTGAGGCAGCAGATTCAGCACCACTCAAGCTTTGCAAGTGCCCTGGAGTCGCTGACCGAGTT
  CTCCAGAAGACACCAGAAAGTTACTAATAGATCCAAAAAAGACGAAAAATGTACAAAAGACAATGAGCCT
  ACTCTGGAAGTGGCCTGTTTGGAGGATAGCACTTTTACAAGTGTGTCAGAATCAAGTCATCAGTCACCAG
  AAAACTCGACAGAAGAAGCAGAAGTGTGTGTCCAGGAGCTTCAGTGTCCCCAGGTGGCTACTAAAAGCCC
  CTCAGTAGCAAGCCACGGTGTTTCCAGAAGGAAGAAAAGTGATCCAGGACTAAGAGGAGAGTCTCTTCAG
  ACAATGAAAACAGAAAAAAATGAGATTTCCCGATCTTCAAGCCCAACTTTTGGAAAGCAAACAGAGCCAG
  TCAACTGGCCTATCTTTAATTCTCTAAGACGGCATTTCAATAGTAAAGTAAAAAATTGCACACCAGTACT
  CAAGTCATCAAAGAACCGTGCCCCTGGTTCTTCCACTTTCAATAAAACTGCTTTGCCACTCACAGGTAAC
  TGTGTGCCCTCAAACGAAACAGCAGACACATCACTGGGACCTTGCCTTCAGTCAGAAGTCATAATTTCAC
  CAGTGAAGATTGAAGCTACTCCTGCTACGAATTATTCCAAACAGGTGTTCTGCTGTGAGAAAGACCTACT
  CCCGGATACTGAATTGTCGCCAGGAACTGAAGAAGCCAAGTGTCCCAGCTCAAACAAAGCTGCTGAAACA
  GAAGTGGATGATGACTCTGAGTGTTTTACACCTGAACTTTTTGATCCTGTCGATACCAACGAAGAAAACG
  GTGAACTGGTTGAAACTGATAGATCAAGTCATAGTTCAGATTGTTTTTCAGCGGAAGAGCTTTTTGAAAC
  TGCAACTGGATTTGGGCAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211750 representing NM_178309
  Red=Cloning site Green=Tags(s)
  
  MSSVLSDYTIGGVKIHFPCRAYPAQLAMMNSIVRGLNSSQHCLLESPTGSGKSLALLCSALAWQQSLSEK
  PVDEGLNKKPEAPPSCSCACHSKNFTYSDTNLDTSPHFNSPSKPSSGRNGVSTPCQDSPEKNTLAAKLSA
  KKQASIHRDEDDDFQVEKKRIRPLETTQQIRKRHCLEKDVHHVDARLASEKRVKPESPIGKSFSDRKDSF
  QNVDGLCSRCCCSAKQGNNQEPANTVKKDHGGQCKRPKIYFGTRTHKQIAQITRELRKTAYSGVPMTILS
  SRDHSCVHPEVVGNFNRKEKCMELLDGKHGKSCYFYHGVHKISNQQTLQHLQGMSRAWDIEELVSLGRKL
  KACPYYTARELIEDADIVFCPYNYLLDSQIRETMDIKLKGQVVILDEAHNIEDCARESASYSVTEVQLRF
  ARDELDSLINGNIRKKSHEPLRDVCYNLINWLETNSKHLVERGYESSCKIWSGNEMLLNLYRMGITTATF
  PVLQRHLSAVLQKEEKVTPIHGKEEAIQIPIISASTQVVLKGLFMVLDYLFRENSRFADDYKVAIQQTYS
  WTNQIAIFDKTGVLAVPKNKKHSRQKIGVNALNFWCLNPAVAFSDINDKVRTIVLTSGTLSPLKSFSSEL
  GVTFSIQLEANHVISNSQVWVGTVGSGPKGRNLCATFQHTETFEFQDEVGMLLLSVCQTVSQGILCFLPS
  YKLLEKLRERWIFTGLWHSLESVKTVIAEPQGGEKTDFDELLQVYYDAIKFKGEKDGALLIAVCRGKVSE
  GLDFSDDNARAVITVGIPFPNVKDLQVELKRQYNDHHSKSRGLLPGRQWYEIQAYRALNQALGRCIRHKN
  DWGALILVDDRFNNNPNRYISGLSKWVRQQIQHHSSFASALESLTEFSRRHQKVTNRSKKDEKCTKDNEP
  TLEVACLEDSTFTSVSESSHQSPENSTEEAEVCVQELQCPQVATKSPSVASHGVSRRKKSDPGLRGESLQ
  TMKTEKNEISRSSSPTFGKQTEPVNWPIFNSLRRHFNSKVKNCTPVLKSSKNRAPGSSTFNKTALPLTGN
  CVPSNETADTSLGPCLQSEVIISPVKIEATPATNYSKQVFCCEKDLLPDTELSPGTEEAKCPSSNKAAET
  EVDDDSECFTPELFDPVDTNEENGELVETDRSSHSSDCFSAEELFETATGFGQK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178309
• ORF Size: 3522 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178309.2, NP_840094.1
• RefSeq Size: 6933 bp
• RefSeq ORF: 3525 bp
• Locus ID: 237911
• UniProt ID: Q5SXJ3
• Cytogenetics: 11 C
• MW: 131.8 kDa
• Gene Summary: This gene encodes a member of the DEAH subfamily of DEAD box helicases. A similar protein in humans is both a DNA-dependent ATPase and a 5-prime-to-3-prime DNA helicase, and plays a role in the repair of DNA double stranded breaks through interaction with the breast cancer-associated tumor suppressor BRCA1. [provided by RefSeq, Feb 2011]